Click here to switch to the map view.

The map label for this gene is yacF [H]

Identifier: 52783937

GI number: 52783937

Start: 92263

End: 93264

Strand: Direct

Name: yacF [H]

Synonym: BLi00096

Alternate gene names: 52783937

Gene position: 92263-93264 (Clockwise)

Preceding gene: 52783936

Following gene: 52783938

Centisome position: 2.18

GC content: 47.41

Gene sequence:

>1002_bases
ATGTTTAAAATCGGTGATATAGAATTAAAAAACAGGGTTGTGCTCGCGCCGATGGCCGGTGTCTGCAACTCAGCCTTCCG
CTTGACGGTTAAGGAATTCGGAGCGGGTTTGGTCTGCGCCGAAATGGTCAGCGATAAGGCGATCCTTTACAATAATGCGA
AAACGATGGGAATGCTTTATATCGATGAACGTGAAAAACCGCTCAGCCTGCAGATCTTCGGCGGAGAAAAAGAAACGCTT
GTCGAAGCGGCGAAGTTTGTTGACCAAAACACAACTGCGGATATTATTGATATAAACATGGGATGTCCAGTGCCAAAGAT
CACAAAATGTGATGCAGGAGCCAAATGGCTGCTTGATCCGAATAAAATTTATGAAATGGTTTCAGCTGTCGTGGAGGCTG
TTGACAAGCCTGTGACCGTGAAAATGAGAATGGGTTGGGACGAAGACCATATCTTTGCTGTTGAGAATGCGAGAGCTGTA
GAACGAGCAGGCGGAAAAGCGGTCGCTCTTCACGGACGGACCCGCGTGCAAATGTACGAAGGAACGGCGAACTGGGATAT
TATCAAAGAAGTGAAGCAGTCCGTTTCAATCCCTGTAATCGGAAACGGTGATGTCAAAACTCCGCAAGATGCAAAACGCA
TGCTTGATGAAACAGGGGTGGACGGCGTCATGATCGGCCGTGCAGCGCTGGGTAACCCGTGGATGATTTACCGTACGGTT
CACTATTTGGAAACAGGCGAATTGAAAGATGAGCCGAAAGTGCGTGAGAAAATTTCTGTCTGCAAACTTCACCTTGACCG
ATTAATCGGGCTGAAAGGCGAAAATGTTGCGGTTCGCGAAATGAGAAAGCACGCAGCCTGGTATCTAAAAGGCATAAGAG
GAAACGCAAAGGTCCGGAATGAAATCAACCATTGCGAAACGAGAGATGAGCTCGTTCAGCTTTTAGACGCGTTTACGATT
GAAGCCGAGGCAAAAGAGCTTCAGGATGCAAAAGTAGGATAA

Upstream 100 bases:

>100_bases
CGTATGCCGACGGAGAGCATGCTCAGGGATGTAGCCAATACTTTAAATATAACAGTGGAGGAACTATCTCCATCAGAAGA
ATAAGAAAGGAGGACTAAAA

Downstream 100 bases:

>100_bases
ATCCGACCTCTGCTCACTGCCAGTTTTCACTGGCAGTTTTTCTGCTTTTTTCCTGAAAGACTAAAAGTAACGGAGTGATA
TATGATGAGCCATGAAGAGC

Product: YacF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFKIGDIELKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILYNNAKTMGMLYIDEREKPLSLQIFGGEKETL
VEAAKFVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDPNKIYEMVSAVVEAVDKPVTVKMRMGWDEDHIFAVENARAV
ERAGGKAVALHGRTRVQMYEGTANWDIIKEVKQSVSIPVIGNGDVKTPQDAKRMLDETGVDGVMIGRAALGNPWMIYRTV
HYLETGELKDEPKVREKISVCKLHLDRLIGLKGENVAVREMRKHAAWYLKGIRGNAKVRNEINHCETRDELVQLLDAFTI
EAEAKELQDAKVG

Sequences:

>Translated_333_residues
MFKIGDIELKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILYNNAKTMGMLYIDEREKPLSLQIFGGEKETL
VEAAKFVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDPNKIYEMVSAVVEAVDKPVTVKMRMGWDEDHIFAVENARAV
ERAGGKAVALHGRTRVQMYEGTANWDIIKEVKQSVSIPVIGNGDVKTPQDAKRMLDETGVDGVMIGRAALGNPWMIYRTV
HYLETGELKDEPKVREKISVCKLHLDRLIGLKGENVAVREMRKHAAWYLKGIRGNAKVRNEINHCETRDELVQLLDAFTI
EAEAKELQDAKVG
>Mature_333_residues
MFKIGDIELKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILYNNAKTMGMLYIDEREKPLSLQIFGGEKETL
VEAAKFVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDPNKIYEMVSAVVEAVDKPVTVKMRMGWDEDHIFAVENARAV
ERAGGKAVALHGRTRVQMYEGTANWDIIKEVKQSVSIPVIGNGDVKTPQDAKRMLDETGVDGVMIGRAALGNPWMIYRTV
HYLETGELKDEPKVREKISVCKLHLDRLIGLKGENVAVREMRKHAAWYLKGIRGNAKVRNEINHCETRDELVQLLDAFTI
EAEAKELQDAKVG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=232, Percent_Identity=31.8965517241379, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=32.034632034632, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=31.8777292576419, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI8923374, Length=248, Percent_Identity=29.8387096774194, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=30.0884955752212, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=39.3081761006289, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788462, Length=252, Percent_Identity=34.5238095238095, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17543114, Length=234, Percent_Identity=31.1965811965812, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=30.2631578947368, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=248, Percent_Identity=29.4354838709677, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=29.3023255813954, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=27.3913043478261, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=257, Percent_Identity=25.6809338521401, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=26.7543859649123, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585320, Length=233, Percent_Identity=33.9055793991416, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24580595, Length=255, Percent_Identity=31.3725490196078, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19920448, Length=255, Percent_Identity=31.3725490196078, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45549423, Length=262, Percent_Identity=29.7709923664122, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=30.3964757709251, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37014; Mature: 37014

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIGDIELKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILYNNAKTMGMLY
CCEECCEEECCEEEEECCHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEEECCCEEEEEE
IDEREKPLSLQIFGGEKETLVEAAKFVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDP
EECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCEEEECH
NKIYEMVSAVVEAVDKPVTVKMRMGWDEDHIFAVENARAVERAGGKAVALHGRTRVQMYE
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GTANWDIIKEVKQSVSIPVIGNGDVKTPQDAKRMLDETGVDGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHCCCCEEEECCHHCCCCEEEEEEE
HYLETGELKDEPKVREKISVCKLHLDRLIGLKGENVAVREMRKHAAWYLKGIRGNAKVRN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
EINHCETRDELVQLLDAFTIEAEAKELQDAKVG
HHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFKIGDIELKNRVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILYNNAKTMGMLY
CCEECCEEECCEEEEECCHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEEECCCEEEEEE
IDEREKPLSLQIFGGEKETLVEAAKFVDQNTTADIIDINMGCPVPKITKCDAGAKWLLDP
EECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCEEEECH
NKIYEMVSAVVEAVDKPVTVKMRMGWDEDHIFAVENARAVERAGGKAVALHGRTRVQMYE
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GTANWDIIKEVKQSVSIPVIGNGDVKTPQDAKRMLDETGVDGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHCCCCEEEECCHHCCCCEEEEEEE
HYLETGELKDEPKVREKISVCKLHLDRLIGLKGENVAVREMRKHAAWYLKGIRGNAKVRN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
EINHCETRDELVQLLDAFTIEAEAKELQDAKVG
HHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]