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The map label for this gene is spoVC

Identifier: 52783910

GI number: 52783910

Start: 64059

End: 64625

Strand: Direct

Name: spoVC

Synonym: BLi00066

Alternate gene names: 52783910

Gene position: 64059-64625 (Clockwise)

Preceding gene: 52783909

Following gene: 52783911

Centisome position: 1.52

GC content: 43.74

Gene sequence:

>567_bases
ATGCTTGTGTTTGCAGGATTGGGCAATCCGGGTAAAACATATGAAAATACGAGACACAATGTAGGTTTTATGACGATTGA
CGAGCTCTCGAAAGAGTGGAACATTCCGCTTGATAAAACAAAGTTCAACGGACAATACGGAATCGGGTTTGTTTCCGGCA
AAAAGGTTCTACTTGTTAAGCCGCTTACATATATGAATTTATCGGGAGAATGTTTGAGGCCGCTTTTGGACTATTACGAG
ATCCCTGTTGACAATTTGAAAGTGATTTACGATGATTTGGATCTTCCGACCGGAAGAATCCGTCTGAGGACGAAAGGAAG
CGCAGGAGGCCATAACGGCATCAAATCGACGATTCAGCATCTGGGAACAAGTGAGTTTAACCGGATCAGAATCGGAATAG
GCCGTCCGGTAAACGGCATGAAAGTCGTCGATTATGTGCTTGGCGCTTTTACAGATGAAGAAGAGCCGGCGATAAAAGAG
GCCGTCAGACAATCGGCCAAGGCCTGTGAAGCTTCTTTGGAAAAACCTTTTTTAGAAGTCATGAATGAATTTAACGCAAA
GGTATAA

Upstream 100 bases:

>100_bases
CCGCCCGCGGTTACGTCTTTTGTGCTAGAATGAGGGGAAATTAGGATGCTCCCCCTCAGTATGCTGAGGCAGGGTTTTGC
TTAAGGGAGGATATAGAATA

Downstream 100 bases:

>100_bases
GGCAAAAGGGAACGGAACATACTAGTCTTAAAAAGACTCGGTATGGAGGTTCCTTTATGGCTTTACATTATTATTGCCGG
CATTGCGGCGTTAAAGTCGG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MLVFAGLGNPGKTYENTRHNVGFMTIDELSKEWNIPLDKTKFNGQYGIGFVSGKKVLLVKPLTYMNLSGECLRPLLDYYE
IPVDNLKVIYDDLDLPTGRIRLRTKGSAGGHNGIKSTIQHLGTSEFNRIRIGIGRPVNGMKVVDYVLGAFTDEEEPAIKE
AVRQSAKACEASLEKPFLEVMNEFNAKV

Sequences:

>Translated_188_residues
MLVFAGLGNPGKTYENTRHNVGFMTIDELSKEWNIPLDKTKFNGQYGIGFVSGKKVLLVKPLTYMNLSGECLRPLLDYYE
IPVDNLKVIYDDLDLPTGRIRLRTKGSAGGHNGIKSTIQHLGTSEFNRIRIGIGRPVNGMKVVDYVLGAFTDEEEPAIKE
AVRQSAKACEASLEKPFLEVMNEFNAKV
>Mature_188_residues
MLVFAGLGNPGKTYENTRHNVGFMTIDELSKEWNIPLDKTKFNGQYGIGFVSGKKVLLVKPLTYMNLSGECLRPLLDYYE
IPVDNLKVIYDDLDLPTGRIRLRTKGSAGGHNGIKSTIQHLGTSEFNRIRIGIGRPVNGMKVVDYVLGAFTDEEEPAIKE
AVRQSAKACEASLEKPFLEVMNEFNAKV

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=156, Percent_Identity=31.4102564102564, Blast_Score=97, Evalue=9e-21,
Organism=Escherichia coli, GI1787455, Length=186, Percent_Identity=37.0967741935484, Blast_Score=107, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321983, Length=184, Percent_Identity=31.5217391304348, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_BACLD (Q65PG8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077339.1
- RefSeq:   YP_089739.1
- ProteinModelPortal:   Q65PG8
- SMR:   Q65PG8
- STRING:   Q65PG8
- EnsemblBacteria:   EBBACT00000057806
- EnsemblBacteria:   EBBACT00000058628
- GeneID:   3027753
- GeneID:   3101218
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00066
- KEGG:   bli:BL00517
- NMPDR:   fig|279010.5.peg.1347
- eggNOG:   COG0193
- GeneTree:   EBGT00050000001189
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   BLIC279010-1:BLI00066-MONOMER
- BioCyc:   BLIC279010:BL00517-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20924; Mature: 20924

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVFAGLGNPGKTYENTRHNVGFMTIDELSKEWNIPLDKTKFNGQYGIGFVSGKKVLLVK
CEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEEC
PLTYMNLSGECLRPLLDYYEIPVDNLKVIYDDLDLPTGRIRLRTKGSAGGHNGIKSTIQH
CHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHH
LGTSEFNRIRIGIGRPVNGMKVVDYVLGAFTDEEEPAIKEAVRQSAKACEASLEKPFLEV
CCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
MNEFNAKV
HHHHCCCC
>Mature Secondary Structure
MLVFAGLGNPGKTYENTRHNVGFMTIDELSKEWNIPLDKTKFNGQYGIGFVSGKKVLLVK
CEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCEEEECCCEEEEEC
PLTYMNLSGECLRPLLDYYEIPVDNLKVIYDDLDLPTGRIRLRTKGSAGGHNGIKSTIQH
CHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHH
LGTSEFNRIRIGIGRPVNGMKVVDYVLGAFTDEEEPAIKEAVRQSAKACEASLEKPFLEV
CCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
MNEFNAKV
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA