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The map label for this gene is yaaE

Identifier: 52783867

GI number: 52783867

Start: 19923

End: 20513

Strand: Direct

Name: yaaE

Synonym: BLi00017

Alternate gene names: 52783867

Gene position: 19923-20513 (Clockwise)

Preceding gene: 52783866

Following gene: 52783868

Centisome position: 0.47

GC content: 49.24

Gene sequence:

>591_bases
ATGCTGACAATTGGTGTACTTGGACTTCAGGGAGCAGTAAGAGAACACATTCGCTCAATTGAAGCATGCGGAGCGGCCGG
AAAAGTCATCAAGTGGCCGGAAGAGTTAAAGGAAATCGACGGTTTGATTTTGCCAGGAGGCGAAAGCACGACGATGAGGC
GCCTCATCGATACGTATCAGTTTATGAAGCCTCTTCAGGAATTTGCCGCATCCGGAAAGCCGGTATTTGGAACTTGTGCT
GGGCTGATTATCCTGGCGAAGAACATTGCCGGAACTAACGATGCCCATCTCGGGGTTCTGGATGTAACGGTAGAGCGGAA
TTCCTTTGGAAGACAGGTGGACAGCTTTGAAGCAGACTTAACCGTTAAAGGACTTGAGGGACCGTTTACAGGCGTATTTA
TCCGCGCTCCGCACATTTTGGAAGCGGGCGCAGATGTAGAAGTGCTTTCGGAACACAACGGAAGAATCGTCGCTGCCAAA
CAAGGAAATCTTCTCGGCTGTTCTTTCCACCCTGAGCTGACGGATGATCACAGAATGACGAAATTATTTGTCGAGATGGT
GGAAAAGCATAAACGGGAAGCCGTCGTATAA

Upstream 100 bases:

>100_bases
AGAGCTTGGAACTGCGATGAAAGGGATTGAAATCTCAAATCTGCTGCCAGAACAGCGTATGCAAGAGCGCGGCTGGTAAA
CACGAAGGAGACGGATTGAC

Downstream 100 bases:

>100_bases
AACGGTTGAAAGCTGTTAAAACTTGTAGTACATTTATAAGACAATATAGATCGAAAAGCGTTGATAGGAAGCAGTAGGGA
GCCTCTCTTTCCAAGAGAGC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MLTIGVLGLQGAVREHIRSIEACGAAGKVIKWPEELKEIDGLILPGGESTTMRRLIDTYQFMKPLQEFAASGKPVFGTCA
GLIILAKNIAGTNDAHLGVLDVTVERNSFGRQVDSFEADLTVKGLEGPFTGVFIRAPHILEAGADVEVLSEHNGRIVAAK
QGNLLGCSFHPELTDDHRMTKLFVEMVEKHKREAVV

Sequences:

>Translated_196_residues
MLTIGVLGLQGAVREHIRSIEACGAAGKVIKWPEELKEIDGLILPGGESTTMRRLIDTYQFMKPLQEFAASGKPVFGTCA
GLIILAKNIAGTNDAHLGVLDVTVERNSFGRQVDSFEADLTVKGLEGPFTGVFIRAPHILEAGADVEVLSEHNGRIVAAK
QGNLLGCSFHPELTDDHRMTKLFVEMVEKHKREAVV
>Mature_196_residues
MLTIGVLGLQGAVREHIRSIEACGAAGKVIKWPEELKEIDGLILPGGESTTMRRLIDTYQFMKPLQEFAASGKPVFGTCA
GLIILAKNIAGTNDAHLGVLDVTVERNSFGRQVDSFEADLTVKGLEGPFTGVFIRAPHILEAGADVEVLSEHNGRIVAAK
QGNLLGCSFHPELTDDHRMTKLFVEMVEKHKREAVV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=207, Percent_Identity=40.5797101449275, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=36.7924528301887, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_BACLD (Q65PL1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077294.1
- RefSeq:   YP_089696.1
- ProteinModelPortal:   Q65PL1
- SMR:   Q65PL1
- STRING:   Q65PL1
- EnsemblBacteria:   EBBACT00000055292
- EnsemblBacteria:   EBBACT00000059853
- GeneID:   3029321
- GeneID:   3101004
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00017
- KEGG:   bli:BL02354
- NMPDR:   fig|279010.5.peg.1202
- eggNOG:   COG0311
- GeneTree:   EBGT00050000000582
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   BLIC279010-1:BLI00017-MONOMER
- BioCyc:   BLIC279010:BL02354-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21259; Mature: 21259

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 79-79 ACT_SITE 170-170 ACT_SITE 172-172 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIGVLGLQGAVREHIRSIEACGAAGKVIKWPEELKEIDGLILPGGESTTMRRLIDTYQ
CEEEEEECHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHCCEEECCCCCHHHHHHHHHHH
FMKPLQEFAASGKPVFGTCAGLIILAKNIAGTNDAHLGVLDVTVERNSFGRQVDSFEADL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHCCCCCCE
TVKGLEGPFTGVFIRAPHILEAGADVEVLSEHNGRIVAAKQGNLLGCSFHPELTDDHRMT
EEECCCCCCCEEEEECCHHHCCCCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHH
KLFVEMVEKHKREAVV
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTIGVLGLQGAVREHIRSIEACGAAGKVIKWPEELKEIDGLILPGGESTTMRRLIDTYQ
CEEEEEECHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHCCEEECCCCCHHHHHHHHHHH
FMKPLQEFAASGKPVFGTCAGLIILAKNIAGTNDAHLGVLDVTVERNSFGRQVDSFEADL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHCCCCCCE
TVKGLEGPFTGVFIRAPHILEAGADVEVLSEHNGRIVAAKQGNLLGCSFHPELTDDHRMT
EEECCCCCCCEEEEECCHHHCCCCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHHH
KLFVEMVEKHKREAVV
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA