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The map label for this gene is gyrB [H]

Identifier: 52783861

GI number: 52783861

Start: 4796

End: 6709

Strand: Direct

Name: gyrB [H]

Synonym: BLi00006

Alternate gene names: 52783861

Gene position: 4796-6709 (Clockwise)

Preceding gene: 52783860

Following gene: 52783862

Centisome position: 0.11

GC content: 47.18

Gene sequence:

>1914_bases
ATGGAACAGCAAAATAATTACGATGAAAATCAGATACAGGTTCTAGAGGGATTGGAAGCCGTTCGGAAAAGACCCGGAAT
GTACATCGGGTCGACAAGCGGCAAAGGTCTGCACCATTTAGTATGGGAAATTGTCGATAACAGCATCGATGAAGCATTGG
CCGGTTACTGCACTGAAATCAATGTCGCAATTGAAAAAGACAACAGCATCACAGTAAAAGACAACGGACGGGGTATCCCG
GTCGGTATTCATGAGAAGATGGGTCGTCCCGCTGTGGAAGTCATCATGACTGTCCTGCACGCCGGAGGAAAGTTTGACGG
AAGCGGATATAAAGTTTCGGGCGGTTTGCACGGCGTCGGTGCTTCTGTTGTTAACGCCCTTTCAACCGAGCTGGATGTAA
CGGTTTACAGAGATGGAAAAATCCATTATCAGGAATTTGAACGTGGCGTTCCGAAAGCTGATTTGAAAGTCATTGGAGAT
ACGGAAGTGACGGGAACGACCACACACTTCAAGCCTGATCCGGAAATATTCACGGAAACGACTGAATACGACTATGATAC
GCTCGCCACTCGTGTCCGCGAACTCGCTTTCTTGACAAAAGGCGTCAAAATCACGATCGAAGACAAGCGAGAAGGAAAAG
AACGCAAGAATGAATACTGCTATGAAGGCGGTATTAAAAGCTATGTTGAACACTTGAACCGTTCGCGGGAAGTTATTCAT
GAAGAGCCGGTCTATATTGAAGGATCCAAAGACGGCATTACAGTCGAGGTGGCTCTTCAATACAATGACAGCTATACAAG
CAACATTTATTCATTTGCTAACAACATTCATACGTATGAAGGCGGAACCCATGAAGCCGGCTTTAAGACCGGTTTGACGA
GGGTCATCAATGATTACGCGAGAAGAAACGGCGTATTCAAAGAAAGCGATCCGAACTTAAGCGGAGAAGACGTCCGGGAA
GGTTTGACAGCGATTATTTCAATCAAGCACCCGGATCCTCAATTTGAAGGGCAGACGAAAACAAAGCTCGGCAACTCAGA
AGCGCGTACGATAACAGATGCGCTATTTTCAGAAGCGCTTGAAAAGTTTCTGCTAGAAAACCCGGATTCAGCGAAAAAAA
TCGTTGAAAAAGGGGTTATGGCCGCCAGAGCACGGATGGCTGCAAAGAAAGCACGCGAATTGACGCGCAGAAAAAGCGCC
CTTGAAGTGTCCAATCTGCCGGGGAAACTTGCTGACTGTTCTTCTAAAGACCCGACGATTTCCGAACTTTACATCGTTGA
GGGTGACTCTGCGGGCGGATCGGCAAAACAGGGCCGCGACCGTCATTTCCAAGCAATTTTGCCTTTGAGAGGGAAAATTT
TGAACGTCGAAAAAGCCCGCCTGGACAAAATATTGTCCAACAATGAGGTTCGTTCTATGATCACCGCCCTTGGCACCGGA
ATCGGGGAAGATTTCAACCTTGAAAAAGCCCGCTACCACAAAGTCGTGATTATGACGGATGCCGACGTCGATGGTGCACA
CATCAGAACATTGCTGCTGACCTTTTTCTACCGCTACATGAGACAGATTATCGAACAAGGATATGTCTACATCGCTCAGC
CGCCTCTTTACAAAGTACAGCAGGGGAAACGCGTTGAGTATGCGTACAATGACCAGCAGCTCGAAGAAATTTTAAAAGAG
CTTCCGCAAAATCCTAAGCCAGGCCTGCAGCGCTACAAAGGTTTGGGCGAGATGAATGCGACACAGCTTTGGGAAACGAC
GATGGATCCAGAAACGCGGACATTGCTTCAAGTAACGCTCAAAGATGCGATTGATGCGGATGAAACCTTTGAAATGCTGA
TGGGCGACAAGGTTGAACCGCGCCGCAACTTCATCGAAGAGAATGCCCGATATGTAAAGAATTTGGATATTTAA

Upstream 100 bases:

>100_bases
TGAAAAAACGGGCGCAATCCAAGCCGGAAATCGATTCATAAAGAATCTTAACTATAAATTTATTATGTTTGAAGAAAAGT
GTAGGTGAATGTGCGTGGCA

Downstream 100 bases:

>100_bases
TGATTGTAAGACCTTATTTTCGCTTAATTGAAATAAGGTCTTTTCTAGAATGAGCCTTTGGCGCTCTAGTCATTTGATGT
GTATAAAATCAAGATTATTG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MEQQNNYDENQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCTEINVAIEKDNSITVKDNGRGIP
VGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVGASVVNALSTELDVTVYRDGKIHYQEFERGVPKADLKVIGD
TEVTGTTTHFKPDPEIFTETTEYDYDTLATRVRELAFLTKGVKITIEDKREGKERKNEYCYEGGIKSYVEHLNRSREVIH
EEPVYIEGSKDGITVEVALQYNDSYTSNIYSFANNIHTYEGGTHEAGFKTGLTRVINDYARRNGVFKESDPNLSGEDVRE
GLTAIISIKHPDPQFEGQTKTKLGNSEARTITDALFSEALEKFLLENPDSAKKIVEKGVMAARARMAAKKARELTRRKSA
LEVSNLPGKLADCSSKDPTISELYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTG
IGEDFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIEQGYVYIAQPPLYKVQQGKRVEYAYNDQQLEEILKE
LPQNPKPGLQRYKGLGEMNATQLWETTMDPETRTLLQVTLKDAIDADETFEMLMGDKVEPRRNFIEENARYVKNLDI

Sequences:

>Translated_637_residues
MEQQNNYDENQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCTEINVAIEKDNSITVKDNGRGIP
VGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVGASVVNALSTELDVTVYRDGKIHYQEFERGVPKADLKVIGD
TEVTGTTTHFKPDPEIFTETTEYDYDTLATRVRELAFLTKGVKITIEDKREGKERKNEYCYEGGIKSYVEHLNRSREVIH
EEPVYIEGSKDGITVEVALQYNDSYTSNIYSFANNIHTYEGGTHEAGFKTGLTRVINDYARRNGVFKESDPNLSGEDVRE
GLTAIISIKHPDPQFEGQTKTKLGNSEARTITDALFSEALEKFLLENPDSAKKIVEKGVMAARARMAAKKARELTRRKSA
LEVSNLPGKLADCSSKDPTISELYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTG
IGEDFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIEQGYVYIAQPPLYKVQQGKRVEYAYNDQQLEEILKE
LPQNPKPGLQRYKGLGEMNATQLWETTMDPETRTLLQVTLKDAIDADETFEMLMGDKVEPRRNFIEENARYVKNLDI
>Mature_637_residues
MEQQNNYDENQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCTEINVAIEKDNSITVKDNGRGIP
VGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVGASVVNALSTELDVTVYRDGKIHYQEFERGVPKADLKVIGD
TEVTGTTTHFKPDPEIFTETTEYDYDTLATRVRELAFLTKGVKITIEDKREGKERKNEYCYEGGIKSYVEHLNRSREVIH
EEPVYIEGSKDGITVEVALQYNDSYTSNIYSFANNIHTYEGGTHEAGFKTGLTRVINDYARRNGVFKESDPNLSGEDVRE
GLTAIISIKHPDPQFEGQTKTKLGNSEARTITDALFSEALEKFLLENPDSAKKIVEKGVMAARARMAAKKARELTRRKSA
LEVSNLPGKLADCSSKDPTISELYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTG
IGEDFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIEQGYVYIAQPPLYKVQQGKRVEYAYNDQQLEEILKE
LPQNPKPGLQRYKGLGEMNATQLWETTMDPETRTLLQVTLKDAIDADETFEMLMGDKVEPRRNFIEENARYVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=615, Percent_Identity=27.6422764227642, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI19913408, Length=639, Percent_Identity=27.0735524256651, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI48994957, Length=554, Percent_Identity=59.7472924187726, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=40.7643312101911, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=668, Percent_Identity=25.1497005988024, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=598, Percent_Identity=24.9163879598662, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=26.7942583732057, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=661, Percent_Identity=26.7776096822995, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136538, Length=670, Percent_Identity=25.3731343283582, Blast_Score=132, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71437; Mature: 71437

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQNNYDENQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCTEI
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
NVAIEKDNSITVKDNGRGIPVGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVG
EEEEECCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
ASVVNALSTELDVTVYRDGKIHYQEFERGVPKADLKVIGDTEVTGTTTHFKPDPEIFTET
HHHHHHHHCCEEEEEEECCCEEHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCC
TEYDYDTLATRVRELAFLTKGVKITIEDKREGKERKNEYCYEGGIKSYVEHLNRSREVIH
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
EEPVYIEGSKDGITVEVALQYNDSYTSNIYSFANNIHTYEGGTHEAGFKTGLTRVINDYA
CCCEEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHH
RRNGVFKESDPNLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARTITDALFSEAL
HHCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
EKFLLENPDSAKKIVEKGVMAARARMAAKKARELTRRKSALEVSNLPGKLADCSSKDPTI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SELYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTG
EEEEEEECCCCCCCCCCCCCCHHEEEECCCCEEECHHHHHHHHHHCCHHHHHHHHHHHCC
IGEDFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIEQGYVYIAQPPLYKVQ
CCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHC
QGKRVEYAYNDQQLEEILKELPQNPKPGLQRYKGLGEMNATQLWETTMDPETRTLLQVTL
CCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHH
KDAIDADETFEMLMGDKVEPRRNFIEENARYVKNLDI
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQQNNYDENQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCTEI
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
NVAIEKDNSITVKDNGRGIPVGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVG
EEEEECCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
ASVVNALSTELDVTVYRDGKIHYQEFERGVPKADLKVIGDTEVTGTTTHFKPDPEIFTET
HHHHHHHHCCEEEEEEECCCEEHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCC
TEYDYDTLATRVRELAFLTKGVKITIEDKREGKERKNEYCYEGGIKSYVEHLNRSREVIH
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHC
EEPVYIEGSKDGITVEVALQYNDSYTSNIYSFANNIHTYEGGTHEAGFKTGLTRVINDYA
CCCEEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHH
RRNGVFKESDPNLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARTITDALFSEAL
HHCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
EKFLLENPDSAKKIVEKGVMAARARMAAKKARELTRRKSALEVSNLPGKLADCSSKDPTI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SELYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTG
EEEEEEECCCCCCCCCCCCCCHHEEEECCCCEEECHHHHHHHHHHCCHHHHHHHHHHHCC
IGEDFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIEQGYVYIAQPPLYKVQ
CCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHC
QGKRVEYAYNDQQLEEILKELPQNPKPGLQRYKGLGEMNATQLWETTMDPETRTLLQVTL
CCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHH
KDAIDADETFEMLMGDKVEPRRNFIEENARYVKNLDI
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]