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The map label for this gene is dnaA [H]

Identifier: 52783856

GI number: 52783856

Start: 311

End: 1651

Strand: Direct

Name: dnaA [H]

Synonym: BLi00001

Alternate gene names: 52783856

Gene position: 311-1651 (Clockwise)

Preceding gene: NA

Following gene: 52783857

Centisome position: 0.01

GC content: 43.18

Gene sequence:

>1341_bases
ATGAAAAACATATCGGATCTTTGGAATCAAGCTTTGGGGCAGATCGAAAAAAAATTGAGCAAGCCCAGCTTTGAAACATG
GATGAAATCGACAAAGGCCCATTCATTGCAGGGCGATACGCTGATCATCACCGCACCGAACGAGTTCGCCAGAGACTGGC
TTGAATCAAGATACCTGCACCTGATCGCCGATACGATCTATGATCTGACAGGAGAAGAATTGAGCATTAAATTTGTCATT
CCTCAGAATCAAAATGAAGAAGATTTTATGCCAAAGTCTCCAATCAAAAAAATGTCGAAAGAAGAACCGGCTGATTTTCC
GCAAAACATGCTGAATCCCAAATATACATTTGATACGTTCGTTATCGGTTCAGGAAACCGATTCGCCCACGCAGCGTCTT
TGGCAGTGGCTGAAGCCCCGGCGAAAGCTTACAATCCGCTGTTTATTTACGGGGGAGTCGGACTTGGAAAGACTCACTTA
ATGCATGCGATCGGGCACTATGTCATCGATCACAATCCATCTGCAAAAGTGGTTTATTTGTCATCTGAGAAATTTACAAA
TGAGTTCATTAACTCGATCCGTGACAATAAAGCTGTCGATTTTCGCAATCGCTATCGAAATGTTGACGTTCTTTTAATAG
ACGATATTCAATTTTTAGCCGGAAAAGAACAGACGCAAGAGGAATTTTTCCATACGTTTAATACGCTGCATGAAGAAACA
AAGCAGATTGTCATTTCCAGCGACCGGCCTCCAAAAGAGATCCCAACGCTTGAAGACCGTTTGCGCTCCCGTTTTGAATG
GGGATTGATCACTGACATCACGCCTCCTGATCTGGAAACAAGAATTGCGATTTTAAGAAAGAAAGCAAAAGCAGAAGGAC
TTGATATCCCGAATGAAGTCATGCTTTATATTGCCAATCAGATCGACAGCAATATCAGGGAGCTGGAAGGGGCATTAATC
AGGGTTGTCGCATATTCTTCCTTGATCAATAAAGACATTAACGCCGATCTGGCTGCTGAAGCTTTGAAAGATATCATTCC
TTCTTCAAAGCCGAAAGTCATTACGATCAAAGACATCCAAAGAATCGTCGGCCAGCAGTTTAATATCAAGCTGGAGGATT
TCAAGGCGAAGAAACGGACAAAATCGGTGGCTTTTCCGCGGCAGATCGCTATGTATCTATCAAGAGAAATGACAGATTCT
TCTCTTCCGAAGATCGGCGAAGAATTTGGCGGACGCGACCACACGACGGTCATCCATGCCCATGAGAAAATATCAAAACT
GCTGAGCGATGATGAACAGCTTCAGCAGCAGATTAAAGAAATTAAAGAGCAGCTGAGATAA

Upstream 100 bases:

>100_bases
ATTTCATTAACGAATGGACTCATCCATTTGCTCTTTTTTTGTGTTCTATAACAGGTTGGACAAGCAAATATTGCTGGTAA
AGGAGGGACGAACCGCCATT

Downstream 100 bases:

>100_bases
GTGATGAGCGGGAAAGTGTGAATAACTTGAACATGGCCTTACACAGTCTGTCCACATGTGGATAGGCTGTGTTTCCGTTC
TTTTTTACACTTATCCACAA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKNISDLWNQALGQIEKKLSKPSFETWMKSTKAHSLQGDTLIITAPNEFARDWLESRYLHLIADTIYDLTGEELSIKFVI
PQNQNEEDFMPKSPIKKMSKEEPADFPQNMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHL
MHAIGHYVIDHNPSAKVVYLSSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEET
KQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEVMLYIANQIDSNIRELEGALI
RVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKDIQRIVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDS
SLPKIGEEFGGRDHTTVIHAHEKISKLLSDDEQLQQQIKEIKEQLR

Sequences:

>Translated_446_residues
MKNISDLWNQALGQIEKKLSKPSFETWMKSTKAHSLQGDTLIITAPNEFARDWLESRYLHLIADTIYDLTGEELSIKFVI
PQNQNEEDFMPKSPIKKMSKEEPADFPQNMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHL
MHAIGHYVIDHNPSAKVVYLSSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEET
KQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEVMLYIANQIDSNIRELEGALI
RVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKDIQRIVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDS
SLPKIGEEFGGRDHTTVIHAHEKISKLLSDDEQLQQQIKEIKEQLR
>Mature_446_residues
MKNISDLWNQALGQIEKKLSKPSFETWMKSTKAHSLQGDTLIITAPNEFARDWLESRYLHLIADTIYDLTGEELSIKFVI
PQNQNEEDFMPKSPIKKMSKEEPADFPQNMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHL
MHAIGHYVIDHNPSAKVVYLSSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEET
KQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEVMLYIANQIDSNIRELEGALI
RVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKDIQRIVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDS
SLPKIGEEFGGRDHTTVIHAHEKISKLLSDDEQLQQQIKEIKEQLR

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=461, Percent_Identity=42.9501084598698, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI226510964, Length=201, Percent_Identity=24.8756218905473, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 50924; Mature: 50924

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNISDLWNQALGQIEKKLSKPSFETWMKSTKAHSLQGDTLIITAPNEFARDWLESRYLH
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
LIADTIYDLTGEELSIKFVIPQNQNEEDFMPKSPIKKMSKEEPADFPQNMLNPKYTFDTF
HHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCHHHHCCCCEEEEEE
VIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAIGHYVIDHNPSAKVVYL
EEECCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
SSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEET
CCHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHH
KQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEV
HHEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
MLYIANQIDSNIRELEGALIRVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKDIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHHH
RIVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDSSLPKIGEEFGGRDHTTVIHA
HHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEH
HEKISKLLSDDEQLQQQIKEIKEQLR
HHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNISDLWNQALGQIEKKLSKPSFETWMKSTKAHSLQGDTLIITAPNEFARDWLESRYLH
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
LIADTIYDLTGEELSIKFVIPQNQNEEDFMPKSPIKKMSKEEPADFPQNMLNPKYTFDTF
HHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCHHHHCCCCEEEEEE
VIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAIGHYVIDHNPSAKVVYL
EEECCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
SSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEET
CCHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHH
KQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEV
HHEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
MLYIANQIDSNIRELEGALIRVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKDIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHHH
RIVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDSSLPKIGEEFGGRDHTTVIHA
HHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEH
HEKISKLLSDDEQLQQQIKEIKEQLR
HHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA