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The map label for this gene is dnaN [H]

Identifier: 52783857

GI number: 52783857

Start: 1828

End: 2964

Strand: Direct

Name: dnaN [H]

Synonym: BLi00002

Alternate gene names: 52783857

Gene position: 1828-2964 (Clockwise)

Preceding gene: 52783856

Following gene: 52783858

Centisome position: 0.04

GC content: 44.59

Gene sequence:

>1137_bases
ATGAAGTTTACAATTCAAAAAGACCGCCTGGTCGAAAGTGTCCAAGATGTGTTAAAAGCCGTTTCTTCAAGAACGACGAT
TCCGATCTTAACCGGTATTAAAATCGTGGCCTCTGATGAAGGGGTCTCTCTGACAGGCAGCGATTCCGATATTTCGATTG
AATCGTTTATCCCGAAAGAAGACGGCGATTTAGAGATCGTGACAATTGAACAGCCCGGCAGCATTGTGCTTCAAGCCCGT
TTTTTCAGTGAAATTGTCAAAAAGCTGCCGATGTCAACAGTGGAAATCGAGGTTCAAAATCAATACTTAACGATCATCCG
CTCCGGCAAAGCAGAGTTTAACTTAAACGGTTTGGATGCAAGCGAATATCCGCTTTTGCCGCAAATTGAAGAGCATCACG
CTTTTCAAATTCCGACCGATCTGCTGAAAAACCTGATCCGCCAAACCGTTTTTGCAGTGTCCACCTCAGAAACACGCCCA
ATCTTGACAGGTGTAAACTGGAATGTCACTGGCGGTGAATTAATATGCACTGCAACGGATAGTCATCGTCTTGCGCTAAG
GAAAGCTAAGCTCGACATTAACGAAGACAGTTCATACAATGTCGTCATCCCAGGAAAAAGCTTAACCGAGCTCAGCAAAA
TCCTTGATGACCATCAGGAGCTTGTTGATATTGTGATTACCGAAACACAAGTGCTGTTTAAAACAAAAAACGTTCTGTTT
TTCTCCAGGCTTCTTGACGGAAACTATCCGGATACGAACCGCCTGATTCCTCAGGAAAGCAAAACGAACTTGATTGTCAA
TACTAAGGAATTTCTCCAGGCGATCGACAGGGCTTCGCTTTTGGCGAGAGAAGGACGAAACAACGTGGTGAAACTTTCCG
CAGCCGCCAATGAGTCGATCGAAATTTCTTCAAACTCTCCGGAAATCGGAAAGGTTGTTGAAACGGTGAATGCCGAGCAG
ATCGAAGGGGAAGACTTAAAGATATCCTTTAGTCCGAAATATATGCTGGATGCCCTTAAAGTTCTTGAAGGAGAGGACAT
TCATGTAAGCTTCACAGGCGCTATGAGGCCTTTTCTGATCCGTACGCCGAATGACGATTCGATCGTCCAATTAATTCTTC
CTGTCCGGACGTATTAA

Upstream 100 bases:

>100_bases
GTTCTTTTTTACACTTATCCACAAATCCACAGCCCCTACTAGTACTTCTGCTATTTTTATAAAACATAATAATTAATAGA
TTCCCGCAAGGAGGATGATT

Downstream 100 bases:

>100_bases
TTTGTTGGAATAGAAAGTTGCCGGCTGGTCCGGCAGCTTCTCTTATTTTTTCTGGAGAAAAGCGCTCTCGCCCTGCCTTG
ATTGCTCATACCGCAAGTTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKFTIQKDRLVESVQDVLKAVSSRTTIPILTGIKIVASDEGVSLTGSDSDISIESFIPKEDGDLEIVTIEQPGSIVLQAR
FFSEIVKKLPMSTVEIEVQNQYLTIIRSGKAEFNLNGLDASEYPLLPQIEEHHAFQIPTDLLKNLIRQTVFAVSTSETRP
ILTGVNWNVTGGELICTATDSHRLALRKAKLDINEDSSYNVVIPGKSLTELSKILDDHQELVDIVITETQVLFKTKNVLF
FSRLLDGNYPDTNRLIPQESKTNLIVNTKEFLQAIDRASLLAREGRNNVVKLSAAANESIEISSNSPEIGKVVETVNAEQ
IEGEDLKISFSPKYMLDALKVLEGEDIHVSFTGAMRPFLIRTPNDDSIVQLILPVRTY

Sequences:

>Translated_378_residues
MKFTIQKDRLVESVQDVLKAVSSRTTIPILTGIKIVASDEGVSLTGSDSDISIESFIPKEDGDLEIVTIEQPGSIVLQAR
FFSEIVKKLPMSTVEIEVQNQYLTIIRSGKAEFNLNGLDASEYPLLPQIEEHHAFQIPTDLLKNLIRQTVFAVSTSETRP
ILTGVNWNVTGGELICTATDSHRLALRKAKLDINEDSSYNVVIPGKSLTELSKILDDHQELVDIVITETQVLFKTKNVLF
FSRLLDGNYPDTNRLIPQESKTNLIVNTKEFLQAIDRASLLAREGRNNVVKLSAAANESIEISSNSPEIGKVVETVNAEQ
IEGEDLKISFSPKYMLDALKVLEGEDIHVSFTGAMRPFLIRTPNDDSIVQLILPVRTY
>Mature_378_residues
MKFTIQKDRLVESVQDVLKAVSSRTTIPILTGIKIVASDEGVSLTGSDSDISIESFIPKEDGDLEIVTIEQPGSIVLQAR
FFSEIVKKLPMSTVEIEVQNQYLTIIRSGKAEFNLNGLDASEYPLLPQIEEHHAFQIPTDLLKNLIRQTVFAVSTSETRP
ILTGVNWNVTGGELICTATDSHRLALRKAKLDINEDSSYNVVIPGKSLTELSKILDDHQELVDIVITETQVLFKTKNVLF
FSRLLDGNYPDTNRLIPQESKTNLIVNTKEFLQAIDRASLLAREGRNNVVKLSAAANESIEISSNSPEIGKVVETVNAEQ
IEGEDLKISFSPKYMLDALKVLEGEDIHVSFTGAMRPFLIRTPNDDSIVQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=378, Percent_Identity=28.5714285714286, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41993; Mature: 41993

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIQKDRLVESVQDVLKAVSSRTTIPILTGIKIVASDEGVSLTGSDSDISIESFIPKE
CCEECCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEHHHCCCCC
DGDLEIVTIEQPGSIVLQARFFSEIVKKLPMSTVEIEVQNQYLTIIRSGKAEFNLNGLDA
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCEEEECCCCC
SEYPLLPQIEEHHAFQIPTDLLKNLIRQTVFAVSTSETRPILTGVNWNVTGGELICTATD
CCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEECCCCCCEEECCEEEECCCEEEEEECC
SHRLALRKAKLDINEDSSYNVVIPGKSLTELSKILDDHQELVDIVITETQVLFKTKNVLF
CCEEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCEEEEEECCHHE
FSRLLDGNYPDTNRLIPQESKTNLIVNTKEFLQAIDRASLLAREGRNNVVKLSAAANESI
EHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
EISSNSPEIGKVVETVNAEQIEGEDLKISFSPKYMLDALKVLEGEDIHVSFTGAMRPFLI
EECCCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCEEE
RTPNDDSIVQLILPVRTY
ECCCCCCEEEEEEECCCC
>Mature Secondary Structure
MKFTIQKDRLVESVQDVLKAVSSRTTIPILTGIKIVASDEGVSLTGSDSDISIESFIPKE
CCEECCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEHHHCCCCC
DGDLEIVTIEQPGSIVLQARFFSEIVKKLPMSTVEIEVQNQYLTIIRSGKAEFNLNGLDA
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCEEEECCCCC
SEYPLLPQIEEHHAFQIPTDLLKNLIRQTVFAVSTSETRPILTGVNWNVTGGELICTATD
CCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEECCCCCCEEECCEEEECCCEEEEEECC
SHRLALRKAKLDINEDSSYNVVIPGKSLTELSKILDDHQELVDIVITETQVLFKTKNVLF
CCEEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCEEEEEECCHHE
FSRLLDGNYPDTNRLIPQESKTNLIVNTKEFLQAIDRASLLAREGRNNVVKLSAAANESI
EHHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
EISSNSPEIGKVVETVNAEQIEGEDLKISFSPKYMLDALKVLEGEDIHVSFTGAMRPFLI
EECCCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCEEE
RTPNDDSIVQLILPVRTY
ECCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]