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The map label for this gene is dnaN [H]

Identifier: 52142810

GI number: 52142810

Start: 2567318

End: 2568448

Strand: Direct

Name: dnaN [H]

Synonym: BCZK2430

Alternate gene names: 52142810

Gene position: 2567318-2568448 (Clockwise)

Preceding gene: 52142811

Following gene: 52142809

Centisome position: 48.43

GC content: 32.1

Gene sequence:

>1131_bases
ATGGAGTTTATCGTTAATCACAAACATTTTACACAAGCACTTTCAGATGTAAGTAAAGCCATATCAGCGAAAGCTATAAT
TCCTATATTATCTGGCATAAAAATAACAGCAGATCAATCTGGAATTACTTTAATCGCAAGTAATTCGAATATTTTTATCG
AAAAATTTATACCTAGTGCTATAGATGATAAACAAATTACGACTATCTTACAAGCAGGAACAATTGTTGTACCTGCAAAA
TACTTCATTGAAATTATAAAGAAAATGCCAAGCGATATAGTAATAAAAAGTAAGAATGAGCAAACAATTACCATACAATC
AGGAGAAATCACATTAAACTTAAATGGCTTTCCAGCGAATGAATTTCCTAACGTACCACAAATAGACGGTCATACAGAAA
TACAAATAGAAACAAAACAATTGATTGATGCGTTTAAACAAACAGTCTTTGCAGTAGCGAAAAATGAATCTAGACCCGTT
CTTACTGGCGTACATATCGAGTTAGACCATAACAAATTAATATGCGCTGCAACTGACTCTCATAGACTAGCTATACGTGA
AACACTAATTTCTACTAATATGAAAGCGAATTGTATTGTACCAAGTGCAACCATTAATGAGCTTTTAAAATTAATGAACA
GCAATTTAGAATTCGTTTCTATTTATCTATCAGAAAGTCACATTATTTTTACATTCGGCACAACTACGTTATATTCACGA
CTAATTGAAGGGAAATATCCTAATATTTCTACTCTCATTCCAAATGAATTTCAAACCGTCATTAACATAGATAGACAAAG
AATGTTACAAGGGGTAGATCGATCAAGCTTATTAGCAAGTGAACGGGCAAATAACAATGTTAACTTAGAAATTGTAAACG
AATCTACAATTCAAATTTCTTCTAACGCTTCTCAGATTGGAAAAATATCTGAGACGCAACAGATAGATGTAATTCAAGGT
AAAAAACAATTAAATATATCTTTCGATGGACGATTTATGTTGGATGCTTTAAGGGCAATAAAAGAAGAAACGGTTACTTT
AAGTTTCAGCGGTTCTATGAGACCGATATTAATTGAAGCAGGGACACAATCTGCAGCAATCCATCTTATATCTCCAGTAA
GAGCTTATTAA

Upstream 100 bases:

>100_bases
AATATGTTAAAAAAGAAAGAAACGGTACCTTACTTTTTTAACGGAACTATCGTTCATCAATCTTCATAAAAATATTATTT
CATTTTAGGCGGGGGAAACA

Downstream 100 bases:

>100_bases
AAACGGAGGAAGGAAATACATTATGTACAAGCACACTTTATGTTTTATAAAAAGAAATGAAGAAATACTCATGATAAATA
GAGAATATGACCCTGTAAAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MEFIVNHKHFTQALSDVSKAISAKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDKQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDGHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASERANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Sequences:

>Translated_376_residues
MEFIVNHKHFTQALSDVSKAISAKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDKQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDGHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASERANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY
>Mature_376_residues
MEFIVNHKHFTQALSDVSKAISAKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDKQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDGHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASERANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=24.2105263157895, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41519; Mature: 41519

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIVNHKHFTQALSDVSKAISAKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEECCCCC
IDDKQITTILQAGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPAN
CCCHHHHHHHHCCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCCCCC
EFPNVPQIDGHTEIQIETKQLIDAFKQTVFAVAKNESRPVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHEEEECCCCCCEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
CCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASERANNNVNLEIVNESTIQIS
HHCCCCCCCEEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCEEEEE
SNASQIGKISETQQIDVIQGKKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHCCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC
>Mature Secondary Structure
MEFIVNHKHFTQALSDVSKAISAKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEECCCCC
IDDKQITTILQAGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPAN
CCCHHHHHHHHCCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCCCCC
EFPNVPQIDGHTEIQIETKQLIDAFKQTVFAVAKNESRPVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHEEEECCCCCCEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
CCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASERANNNVNLEIVNESTIQIS
HHCCCCCCCEEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCEEEEE
SNASQIGKISETQQIDVIQGKKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHCCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]