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The map label for this gene is glnA [H]

Identifier: 52141784

GI number: 52141784

Start: 3586289

End: 3587623

Strand: Reverse

Name: glnA [H]

Synonym: BCZK3462

Alternate gene names: 52141784

Gene position: 3587623-3586289 (Counterclockwise)

Preceding gene: 52141782

Following gene: 52141787

Centisome position: 67.68

GC content: 38.95

Gene sequence:

>1335_bases
ATGGCTAGGTACACAAAAGAAGATATTTTCCGTTTGGCGAAAGAAGAGAATGTAAAGTATATCCGATTACAATTTACGGA
CCTTTTAGGAGTAATTAAAAACGTAGAAATTCCAGTGAGCCAATTAACAAAAGCTCTTGATAACAAAATGATGTTTGATG
GATCTTCTATTGAAGGTTTCGTACGTATTGAAGAATCTGATATGTACTTATATCCAGATTTAGATACTTGGGTAATTTTC
CCTTGGACAGCTGAAAAAGGTAAAGTAGCTCGATTAATCTGTGATATTTACAATGCAGATGGCACTCCATTTGAAGGAGA
TCCACGTAACAATTTAAAACGTGTATTAAAAGAAATGGAAGCTTTAGGATTCTCAGATTTCAATCTTGGACCAGAGCCAG
AATTCTTCCTATTCAAAGTAGATGAAAAAGGAAATCCGACATTAGAATTAAACGATAACGGTGGATACTTCGACCTTGCG
CCGATGGATCTAGGGGAAAACTGTCGTCGTGATATCGTTCTTGAACTTGAAGAAATGGGCTTTGAAATTGAAGCGTCTCA
CCATGAGGTTGCTCCAGGGCAACACGAAATTGACTTCAAATATGCAAACGCAATTCGCTCATGTGATGACATTCAAACAT
TCAAACTTGTTGTAAAAACAATTGCTCGTAAACACGGTTTACACGCAACATTTATGCCAAAACCATTATACGGTGTGAAC
GGTTCAGGTATGCACTGTAACTTATCACTATTCAAAAATGGTGAGAACGTATTCTTCGATCAAAACGGTGACTTACAATT
AAGTGATGATGCTCGTCACTTCATCGCAGGTATTTTAAAACACGCACCAGCATTTACAGCGGTAGCAAACCCAACTGTAA
ACTCTTACAAGCGTTTAGTACCTGGATACGAAGCTCCTTGTTACGTAGCATGGTCTGCACAAAACCGTAGCCCATTAGTA
CGTATCCCTGCATCTCGTGGTATTAGTACACGCGTAGAAGTACGTAGTGTTGACCCAGCTGCAAACCCATATTTAGTAAT
GGCTACATTATTAGCTGCAGGTCTTGATGGAATTAAAAACAAATTAACTCCACCAGCTGCAGTAGACCGTAACATCTACG
TAATGACAAAAGAAGAGCGCGAAGAAGCAGGTATCGTTGACTTACCAGCAACATTAGCGCAAGCTTTAGTTACATTACAA
TCTAACGAAGTAATTAGTAACGCATTAGGAGATCACTTACTTGAGCACTTCATTGAAGCGAAGGAAATTGAGTGGGATAT
CTTCCGTACACAAGTTCACCAATGGGAACGCGATCAATATATGTCTCTGTACTAA

Upstream 100 bases:

>100_bases
ATTTAATCGAACTTCATTGCGACAAGGTGATATTTCAAGGTTTTTTCACTAAAGAGAACTGATTCTGAAGGTGTTTAGAG
GGACTAAGGAGGAATTTATA

Downstream 100 bases:

>100_bases
GAGGCTTGAACCCTTGATACGACTGGTATCAAGGGTTTTTTTATTTTGTGGGTAAAACACTTTTATTTGAAAGAAGAGTA
ATTCACCCACAAAACACCCA

Product: glutamate--ammonia ligase (glutamine synthetase, type I)

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MARYTKEDIFRLAKEENVKYIRLQFTDLLGVIKNVEIPVSQLTKALDNKMMFDGSSIEGFVRIEESDMYLYPDLDTWVIF
PWTAEKGKVARLICDIYNADGTPFEGDPRNNLKRVLKEMEALGFSDFNLGPEPEFFLFKVDEKGNPTLELNDNGGYFDLA
PMDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYANAIRSCDDIQTFKLVVKTIARKHGLHATFMPKPLYGVN
GSGMHCNLSLFKNGENVFFDQNGDLQLSDDARHFIAGILKHAPAFTAVANPTVNSYKRLVPGYEAPCYVAWSAQNRSPLV
RIPASRGISTRVEVRSVDPAANPYLVMATLLAAGLDGIKNKLTPPAAVDRNIYVMTKEEREEAGIVDLPATLAQALVTLQ
SNEVISNALGDHLLEHFIEAKEIEWDIFRTQVHQWERDQYMSLY

Sequences:

>Translated_444_residues
MARYTKEDIFRLAKEENVKYIRLQFTDLLGVIKNVEIPVSQLTKALDNKMMFDGSSIEGFVRIEESDMYLYPDLDTWVIF
PWTAEKGKVARLICDIYNADGTPFEGDPRNNLKRVLKEMEALGFSDFNLGPEPEFFLFKVDEKGNPTLELNDNGGYFDLA
PMDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYANAIRSCDDIQTFKLVVKTIARKHGLHATFMPKPLYGVN
GSGMHCNLSLFKNGENVFFDQNGDLQLSDDARHFIAGILKHAPAFTAVANPTVNSYKRLVPGYEAPCYVAWSAQNRSPLV
RIPASRGISTRVEVRSVDPAANPYLVMATLLAAGLDGIKNKLTPPAAVDRNIYVMTKEEREEAGIVDLPATLAQALVTLQ
SNEVISNALGDHLLEHFIEAKEIEWDIFRTQVHQWERDQYMSLY
>Mature_443_residues
ARYTKEDIFRLAKEENVKYIRLQFTDLLGVIKNVEIPVSQLTKALDNKMMFDGSSIEGFVRIEESDMYLYPDLDTWVIFP
WTAEKGKVARLICDIYNADGTPFEGDPRNNLKRVLKEMEALGFSDFNLGPEPEFFLFKVDEKGNPTLELNDNGGYFDLAP
MDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYANAIRSCDDIQTFKLVVKTIARKHGLHATFMPKPLYGVNG
SGMHCNLSLFKNGENVFFDQNGDLQLSDDARHFIAGILKHAPAFTAVANPTVNSYKRLVPGYEAPCYVAWSAQNRSPLVR
IPASRGISTRVEVRSVDPAANPYLVMATLLAAGLDGIKNKLTPPAAVDRNIYVMTKEEREEAGIVDLPATLAQALVTLQS
NEVISNALGDHLLEHFIEAKEIEWDIFRTQVHQWERDQYMSLY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=432, Percent_Identity=27.0833333333333, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1790301, Length=476, Percent_Identity=41.8067226890756, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI87081870, Length=460, Percent_Identity=30, Blast_Score=161, Evalue=7e-41,
Organism=Drosophila melanogaster, GI281360453, Length=324, Percent_Identity=26.5432098765432, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136208, Length=324, Percent_Identity=26.5432098765432, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24580558, Length=324, Percent_Identity=26.5432098765432, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50146; Mature: 50014

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYTKEDIFRLAKEENVKYIRLQFTDLLGVIKNVEIPVSQLTKALDNKMMFDGSSIEGF
CCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLYPDLDTWVIFPWTAEKGKVARLICDIYNADGTPFEGDPRNNLKRVLKEME
EEEECCCEEEECCCCCEEEEEEECCCCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALGFSDFNLGPEPEFFLFKVDEKGNPTLELNDNGGYFDLAPMDLGENCRRDIVLELEEMG
HCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYANAIRSCDDIQTFKLVVKTIARKHGLHATFMPKPLYGVN
EEEECCCCCCCCCCCEECHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHCNLSLFKNGENVFFDQNGDLQLSDDARHFIAGILKHAPAFTAVANPTVNSYKRLV
CCCCEEEEEEEECCCEEEEECCCCEEECCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHC
PGYEAPCYVAWSAQNRSPLVRIPASRGISTRVEVRSVDPAANPYLVMATLLAAGLDGIKN
CCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
KLTPPAAVDRNIYVMTKEEREEAGIVDLPATLAQALVTLQSNEVISNALGDHLLEHFIEA
CCCCCCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KEIEWDIFRTQVHQWERDQYMSLY
HHCHHHHHHHHHHHHCCCHHEECC
>Mature Secondary Structure 
ARYTKEDIFRLAKEENVKYIRLQFTDLLGVIKNVEIPVSQLTKALDNKMMFDGSSIEGF
CCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLYPDLDTWVIFPWTAEKGKVARLICDIYNADGTPFEGDPRNNLKRVLKEME
EEEECCCEEEECCCCCEEEEEEECCCCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALGFSDFNLGPEPEFFLFKVDEKGNPTLELNDNGGYFDLAPMDLGENCRRDIVLELEEMG
HCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYANAIRSCDDIQTFKLVVKTIARKHGLHATFMPKPLYGVN
EEEECCCCCCCCCCCEECHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHCNLSLFKNGENVFFDQNGDLQLSDDARHFIAGILKHAPAFTAVANPTVNSYKRLV
CCCCEEEEEEEECCCEEEEECCCCEEECCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHC
PGYEAPCYVAWSAQNRSPLVRIPASRGISTRVEVRSVDPAANPYLVMATLLAAGLDGIKN
CCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
KLTPPAAVDRNIYVMTKEEREEAGIVDLPATLAQALVTLQSNEVISNALGDHLLEHFIEA
CCCCCCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KEIEWDIFRTQVHQWERDQYMSLY
HHCHHHHHHHHHHHHCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]