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The map label for this gene is nuoB

Identifier: 52140262

GI number: 52140262

Start: 5101316

End: 5101855

Strand: Reverse

Name: nuoB

Synonym: BCZK4999

Alternate gene names: 52140262

Gene position: 5101855-5101316 (Counterclockwise)

Preceding gene: 52140261

Following gene: 52140264

Centisome position: 96.24

GC content: 40.0

Gene sequence:

>540_bases
TTGGAAAAAGAAGGTGTTACAATGGTTATAAATTTTGAGGAATTACATCCAAATGAGCGAGCGGAATTAGAACGAAATAT
CTTTTTTTCTACATTGGAACAGTTGAAAGGATGGGCGCGAAGTAATTCTTTATGGCCGATGACATTCGGACTGGCATGCT
GTGCAATTGAAATGATGGGAGTAGGTTCATCACATTATGATTTAGATCGATTTGGGTCATTTTTTCGGACTTCACCAAGG
CAATCGGACGTTATGATTGTGTCGGGAACGGTAACGAAGAAGATGGCTCCTATTGTTCGGCGCTTATATGACCAAATGCC
TGAACCAAAATGGGTTATTGCAATGGGATCTTGTGCGACAGCAGGTGGTCCATATGTAAATTCTTACGCTGTTGTGAAAG
GTGTAGATCAAATTGTGCCAGTTGACGTGTATATCCCTGGTTGCCCACCAAATCCTGCAGCTTTAATTTATGGAATTAAT
AAATTAAAAGAAAAAATTCGTTACGAAGCGAAGACTGGAAAGCAGGTGACGAATAAATGA

Upstream 100 bases:

>100_bases
TTTTATATCCATGGGCTGTTGCATATGACAACCTCGGTTTATTTGCACTGATTGAAATGCTCATCTTTGTTGTAATGTTG
CTAGTTGGATTAGCGTATGC

Downstream 100 bases:

>100_bases
GTGATCCTAACAAAGACTTAGAGGATTTAAAAAGAGAAGCGGCTAGGCGTGCGAAAGAAGAGGCAAGAAAGCGTCTCGTT
GCGAAACATGGTGTGGAAAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MEKEGVTMVINFEELHPNERAELERNIFFSTLEQLKGWARSNSLWPMTFGLACCAIEMMGVGSSHYDLDRFGSFFRTSPR
QSDVMIVSGTVTKKMAPIVRRLYDQMPEPKWVIAMGSCATAGGPYVNSYAVVKGVDQIVPVDVYIPGCPPNPAALIYGIN
KLKEKIRYEAKTGKQVTNK

Sequences:

>Translated_179_residues
MEKEGVTMVINFEELHPNERAELERNIFFSTLEQLKGWARSNSLWPMTFGLACCAIEMMGVGSSHYDLDRFGSFFRTSPR
QSDVMIVSGTVTKKMAPIVRRLYDQMPEPKWVIAMGSCATAGGPYVNSYAVVKGVDQIVPVDVYIPGCPPNPAALIYGIN
KLKEKIRYEAKTGKQVTNK
>Mature_179_residues
MEKEGVTMVINFEELHPNERAELERNIFFSTLEQLKGWARSNSLWPMTFGLACCAIEMMGVGSSHYDLDRFGSFFRTSPR
QSDVMIVSGTVTKKMAPIVRRLYDQMPEPKWVIAMGSCATAGGPYVNSYAVVKGVDQIVPVDVYIPGCPPNPAALIYGIN
KLKEKIRYEAKTGKQVTNK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=155, Percent_Identity=60, Blast_Score=206, Evalue=1e-53,
Organism=Escherichia coli, GI1788624, Length=151, Percent_Identity=50.9933774834437, Blast_Score=173, Evalue=8e-45,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788834, Length=131, Percent_Identity=38.1679389312977, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=52.9411764705882, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=58.273381294964, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=58.273381294964, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24651058, Length=138, Percent_Identity=60.8695652173913, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BACAH (A0RL90)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897490.1
- ProteinModelPortal:   A0RL90
- SMR:   A0RL90
- STRING:   A0RL90
- EnsemblBacteria:   EBBACT00000067833
- GeneID:   4543208
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4802
- eggNOG:   COG0377
- GeneTree:   EBGT00050000003976
- HOGENOM:   HBG553221
- OMA:   NYDLERF
- ProtClustDB:   PRK06411
- BioCyc:   BTHU412694:BALH_4802-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20001; Mature: 20001

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEGVTMVINFEELHPNERAELERNIFFSTLEQLKGWARSNSLWPMTFGLACCAIEMMG
CCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
VGSSHYDLDRFGSFFRTSPRQSDVMIVSGTVTKKMAPIVRRLYDQMPEPKWVIAMGSCAT
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
AGGPYVNSYAVVKGVDQIVPVDVYIPGCPPNPAALIYGINKLKEKIRYEAKTGKQVTNK
CCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCHHEEECHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEKEGVTMVINFEELHPNERAELERNIFFSTLEQLKGWARSNSLWPMTFGLACCAIEMMG
CCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
VGSSHYDLDRFGSFFRTSPRQSDVMIVSGTVTKKMAPIVRRLYDQMPEPKWVIAMGSCAT
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
AGGPYVNSYAVVKGVDQIVPVDVYIPGCPPNPAALIYGINKLKEKIRYEAKTGKQVTNK
CCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCHHEEECHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA