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The map label for this gene is ykfA [H]

Identifier: 51893946

GI number: 51893946

Start: 3019720

End: 3020679

Strand: Reverse

Name: ykfA [H]

Synonym: STH2808

Alternate gene names: 51893946

Gene position: 3020679-3019720 (Counterclockwise)

Preceding gene: 51893947

Following gene: 51893945

Centisome position: 84.7

GC content: 73.02

Gene sequence:

>960_bases
ATGACGGGTGCCGCGGTGCGCAGACCCCGGCCCGTGCCGCTGGGCGGGACGGTGGGCGTGGTGGCCCCTGCGGGACCGGT
GCCGCCGGAGGCGCTGGAGGAAGGGCTGAGGCGGCTCCGGGCGTGGGGGTACCGAACCGTCGTCGGGGAGGCGGTGCTGG
ACCGGCGGGGTTACCTGGCCGGCGCCGACGACCGGCGGGCGGAGGACTTCAACCGGATGTGGGCCGATCCCGCGGTGGAT
GCGGTGATCTGCGCCCGGGGCGGATACGGGGCGATGCGGATCCTGCGGCGGATCGACTGGGAGCTGATTCGGCGCAACCC
CAAGTTCTTCTGCGGCTTCTCCGACATCACGGCGCTGCATCTGGCCATGGCTCGGGAGGCGGGCCTCGTCACCTTCCACG
GCCCGATGGTCGCGGCCTTTGGCGGCGCGCACGGGTACAACGGTGCTGGGCTGCGCCGGGCGCTGCAGCAGGTGGGGCCG
CTGGGGGAGATGCCATGGCCGGACCCGGCGGAGGAGGGGGCGCCCCGGCCCCTGACGATCCGGTCGGGCGTGGCCGAGGG
TCGGCTGATCGGGGGTAACCTCACCCTGATCGTCTCGCTGCTGGGCACCCCGTGGGAGCCGGACTTCACCGGCTGCATCG
TGCTGCTGGAGGACGTGGATGAGGCTCCCTACAGGGTGGACCGGATGCTGGTCCAGCTGCTGCTGGCCGGCAAACTCCAG
GGGGCGGCCGGCATCCTCTTCGGGGACTCGCCCACGTGCATGCACGGGCCGCCGGACCGGCCCTCGCTGACGCTGCCGGA
GGTGCTGACGGAACTGCTGGGCCCTTTGGGAATCCCTGTCCTCTACGGTTTTCCCTGCGGTCACGGCCCGCACCGGGCAA
CCCTGCCGCTGGGCGTGCGGACCCGGCTGGATGCGGCGGGCGGCACGCTGACGATCCTGGAACCGGCGCTGGAGCCGTAG

Upstream 100 bases:

>100_bases
CCGGTTTGCCATCGATGAGCGAAAGGCCGGGTGAGCGGCCGGCGGGCGGGAGATCGTCCGTCGGGTGTGACCCCACGTGC
AGCGGAGGAGGGGACGCGGG

Downstream 100 bases:

>100_bases
GTCCGGCAGGGCCGCATTTTGCGCGGGGCTGCCCAGGACGGGGTGCGCCGGAGGGCCGGGGAACATAATATAGGGGTAAA
CCGCGGAGAGGAGCATGGGC

Product: putative muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTGAAVRRPRPVPLGGTVGVVAPAGPVPPEALEEGLRRLRAWGYRTVVGEAVLDRRGYLAGADDRRAEDFNRMWADPAVD
AVICARGGYGAMRILRRIDWELIRRNPKFFCGFSDITALHLAMAREAGLVTFHGPMVAAFGGAHGYNGAGLRRALQQVGP
LGEMPWPDPAEEGAPRPLTIRSGVAEGRLIGGNLTLIVSLLGTPWEPDFTGCIVLLEDVDEAPYRVDRMLVQLLLAGKLQ
GAAGILFGDSPTCMHGPPDRPSLTLPEVLTELLGPLGIPVLYGFPCGHGPHRATLPLGVRTRLDAAGGTLTILEPALEP

Sequences:

>Translated_319_residues
MTGAAVRRPRPVPLGGTVGVVAPAGPVPPEALEEGLRRLRAWGYRTVVGEAVLDRRGYLAGADDRRAEDFNRMWADPAVD
AVICARGGYGAMRILRRIDWELIRRNPKFFCGFSDITALHLAMAREAGLVTFHGPMVAAFGGAHGYNGAGLRRALQQVGP
LGEMPWPDPAEEGAPRPLTIRSGVAEGRLIGGNLTLIVSLLGTPWEPDFTGCIVLLEDVDEAPYRVDRMLVQLLLAGKLQ
GAAGILFGDSPTCMHGPPDRPSLTLPEVLTELLGPLGIPVLYGFPCGHGPHRATLPLGVRTRLDAAGGTLTILEPALEP
>Mature_318_residues
TGAAVRRPRPVPLGGTVGVVAPAGPVPPEALEEGLRRLRAWGYRTVVGEAVLDRRGYLAGADDRRAEDFNRMWADPAVDA
VICARGGYGAMRILRRIDWELIRRNPKFFCGFSDITALHLAMAREAGLVTFHGPMVAAFGGAHGYNGAGLRRALQQVGPL
GEMPWPDPAEEGAPRPLTIRSGVAEGRLIGGNLTLIVSLLGTPWEPDFTGCIVLLEDVDEAPYRVDRMLVQLLLAGKLQG
AAGILFGDSPTCMHGPPDRPSLTLPEVLTELLGPLGIPVLYGFPCGHGPHRATLPLGVRTRLDAAGGTLTILEPALEP

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=309, Percent_Identity=33.9805825242718, Blast_Score=140, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34000; Mature: 33869

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAAVRRPRPVPLGGTVGVVAPAGPVPPEALEEGLRRLRAWGYRTVVGEAVLDRRGYLA
CCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
GADDRRAEDFNRMWADPAVDAVICARGGYGAMRILRRIDWELIRRNPKFFCGFSDITALH
CCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LAMAREAGLVTFHGPMVAAFGGAHGYNGAGLRRALQQVGPLGEMPWPDPAEEGAPRPLTI
HHHHHHCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCCCCEEE
RSGVAEGRLIGGNLTLIVSLLGTPWEPDFTGCIVLLEDVDEAPYRVDRMLVQLLLAGKLQ
ECCCCCCEEECCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
GAAGILFGDSPTCMHGPPDRPSLTLPEVLTELLGPLGIPVLYGFPCGHGPHRATLPLGVR
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEECCCCC
TRLDAAGGTLTILEPALEP
EEECCCCCEEEEECCCCCC
>Mature Secondary Structure 
TGAAVRRPRPVPLGGTVGVVAPAGPVPPEALEEGLRRLRAWGYRTVVGEAVLDRRGYLA
CCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
GADDRRAEDFNRMWADPAVDAVICARGGYGAMRILRRIDWELIRRNPKFFCGFSDITALH
CCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LAMAREAGLVTFHGPMVAAFGGAHGYNGAGLRRALQQVGPLGEMPWPDPAEEGAPRPLTI
HHHHHHCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCCCCEEE
RSGVAEGRLIGGNLTLIVSLLGTPWEPDFTGCIVLLEDVDEAPYRVDRMLVQLLLAGKLQ
ECCCCCCEEECCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
GAAGILFGDSPTCMHGPPDRPSLTLPEVLTELLGPLGIPVLYGFPCGHGPHRATLPLGVR
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCEECCCCC
TRLDAAGGTLTILEPALEP
EEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]