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The map label for this gene is nth [H]

Identifier: 51893925

GI number: 51893925

Start: 3003443

End: 3004150

Strand: Reverse

Name: nth [H]

Synonym: STH2787

Alternate gene names: 51893925

Gene position: 3004150-3003443 (Counterclockwise)

Preceding gene: 51893927

Following gene: 51893924

Centisome position: 84.24

GC content: 68.08

Gene sequence:

>708_bases
ATGGCACGCAAGTCCGTGGACACCGAGGCGATTCTGAGGAAATTGGAGGAGATGTACCCGGACGCCAAGTGCGCGCTCAA
CCACCGGAATGCGTTTGAACTGCTGGTGGCGACCGTCCTCTCGGCCCAGTGCACCGACGCCCGGGTCAACATCGTCACCG
CCCGCATCTTCCCCCGCTACAACCGTCCCGAACACTTCGCGGCGCTGTCGGTGGACGAGATCGGCGAGATGATCCGCGAC
TGCGGGCTGTGGAAGAGCAAGGCGAAGAACATCCAGGGGCTCAGCCAGATGCTGCTGGAGAAGCACGGCGGCGAGGTGCC
GTCGACGATGGAGGAGCTGATTCAGTTGCCCGGCGTCGGCCGGAAGACCGCCAACGTGGTGCTCTCCAACGCCTTCGGGA
TTCCGGCGATCGCGGTGGATACCCACGTGTTCCGCGTGGCCAACCGGCTGGGGCTGGCGGAGGCGAAGACGCCCGAGGAG
ACCGAGCGGCAGCTGATGGAGCGGATCCCTCGGGAGTACTGGTCGCAGGCGCACCACTGGCTGATCTACCACGGCCGCCA
GGTGTGCCACGCCCGGAACCCGCAGTGCAGTCAGTGCCCGCTCCTGCCCCACTGCCGGTTCGGCCGGCAGGCCGGGAAGG
GGTCGGTGCAGCGGGCGAGGGTCTCATCGGGTCGTGCGGCCCGGCGGGCGGGGGGACCGGCGCGGTAA

Upstream 100 bases:

>100_bases
CGGGCGTGCGGCGGAACGCGCCGCCCACCCGAAAAGCGGCAGCGGTTCTCCGCGGGATCCCCGCGGGTTCCGGCCGGCGA
ACCGAGACAGGAGTGACAGC

Downstream 100 bases:

>100_bases
CCCCGGCAGCGGCCGCCGAAACGGCTGGAATCCAGCATTCCCCGCCGCAGGAAGACAGGAGATGCCGGTCTGGTTCGCCA
AGTCCTGAAGACCGGCGCAG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MARKSVDTEAILRKLEEMYPDAKCALNHRNAFELLVATVLSAQCTDARVNIVTARIFPRYNRPEHFAALSVDEIGEMIRD
CGLWKSKAKNIQGLSQMLLEKHGGEVPSTMEELIQLPGVGRKTANVVLSNAFGIPAIAVDTHVFRVANRLGLAEAKTPEE
TERQLMERIPREYWSQAHHWLIYHGRQVCHARNPQCSQCPLLPHCRFGRQAGKGSVQRARVSSGRAARRAGGPAR

Sequences:

>Translated_235_residues
MARKSVDTEAILRKLEEMYPDAKCALNHRNAFELLVATVLSAQCTDARVNIVTARIFPRYNRPEHFAALSVDEIGEMIRD
CGLWKSKAKNIQGLSQMLLEKHGGEVPSTMEELIQLPGVGRKTANVVLSNAFGIPAIAVDTHVFRVANRLGLAEAKTPEE
TERQLMERIPREYWSQAHHWLIYHGRQVCHARNPQCSQCPLLPHCRFGRQAGKGSVQRARVSSGRAARRAGGPAR
>Mature_234_residues
ARKSVDTEAILRKLEEMYPDAKCALNHRNAFELLVATVLSAQCTDARVNIVTARIFPRYNRPEHFAALSVDEIGEMIRDC
GLWKSKAKNIQGLSQMLLEKHGGEVPSTMEELIQLPGVGRKTANVVLSNAFGIPAIAVDTHVFRVANRLGLAEAKTPEET
ERQLMERIPREYWSQAHHWLIYHGRQVCHARNPQCSQCPLLPHCRFGRQAGKGSVQRARVSSGRAARRAGGPAR

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=35.672514619883, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI115298650, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI6912520, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI115298654, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI115298652, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI190358497, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI115298648, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=46.7661691542289, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1789331, Length=185, Percent_Identity=28.6486486486486, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=33.7016574585635, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=192, Percent_Identity=29.6875, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=181, Percent_Identity=28.7292817679558, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550361, Length=171, Percent_Identity=32.1637426900585, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26255; Mature: 26123

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKSVDTEAILRKLEEMYPDAKCALNHRNAFELLVATVLSAQCTDARVNIVTARIFPRY
CCCCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
NRPEHFAALSVDEIGEMIRDCGLWKSKAKNIQGLSQMLLEKHGGEVPSTMEELIQLPGVG
CCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
RKTANVVLSNAFGIPAIAVDTHVFRVANRLGLAEAKTPEETERQLMERIPREYWSQAHHW
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
LIYHGRQVCHARNPQCSQCPLLPHCRFGRQAGKGSVQRARVSSGRAARRAGGPAR
EHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure 
ARKSVDTEAILRKLEEMYPDAKCALNHRNAFELLVATVLSAQCTDARVNIVTARIFPRY
CCCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
NRPEHFAALSVDEIGEMIRDCGLWKSKAKNIQGLSQMLLEKHGGEVPSTMEELIQLPGVG
CCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
RKTANVVLSNAFGIPAIAVDTHVFRVANRLGLAEAKTPEETERQLMERIPREYWSQAHHW
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
LIYHGRQVCHARNPQCSQCPLLPHCRFGRQAGKGSVQRARVSSGRAARRAGGPAR
EHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]