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The map label for this gene is liaS [H]

Identifier: 51893867

GI number: 51893867

Start: 2930697

End: 2931803

Strand: Reverse

Name: liaS [H]

Synonym: STH2729

Alternate gene names: 51893867

Gene position: 2931803-2930697 (Counterclockwise)

Preceding gene: 51893868

Following gene: 51893866

Centisome position: 82.21

GC content: 73.62

Gene sequence:

>1107_bases
ATGGAAGGGCGCATGGAAGAACGACGCACGCTGGGGCTGCAGTGGCAGCTGGTGGCGGTTACCGCCGCGGCCGGCCTGCT
GGCCAGCGGTCTGGCGGTCTTCGGCGTCTGGGTCGCCCTCCAGCAGGGCTTTGGATGGAACGAGGCGCTGGTGGCTGGCG
TCGCCGCCGGCGTCGCGGGCGGGCTCGCCGCCGCCGCATGGAGCTTCCAGCTGGCCAGGGGCATCAAGCGTCGGCTGTGG
AACGCCGGGGACCTCGCCGCACGCATCCGCCGGGGCGATCTCTCGGCCCGGCTGCCGGTGAGTGACGCCGACGAGATCGG
GGAGCTGGAGATGCAGCTCAACGAGATGGCCGCCTACCTGGAGCAAGCGGTGGGCCAGCTCTCCCGGCTGGCGGAGCAGA
ACCGGCTGCTGGCCGAGGAGGCGGGGCGGGGCGCCGCCCTGGAGGAACGGGCCCGCATCGCACGGGACCTGCACGACACG
GTGAACCAGCAGCTCTTCGTGCTCTCGCTCCGGGCCGCGGCGGTCCGGAAGCGGGTCGGCGCCGCGAGCGGTGCCGCAGC
CGGCGGGGATGGCGCCGGGGTTGAACCGCAGCTCGTGACGGAGCTGGCCAACCTGGAGGAATTGGCCCGCACGGCCCACA
GCCAGATCCGGGAGCTGATCCTGCAGCTCCGTCCGACGACGCTGGAGCAGCACGGCCTGGGGCCGGCGCTGGCGGAGTAC
GTGAAGACGGTGGCGGAGCGGGAGAACTGGGCCGTCGAGAACGAGATCGACCAGGCCCTGCGGCTCCGGGGCGCTGAGGG
CGAGGCGCTCTTCCGGGTGGCGCAGGAGGCCCTGAACAACGTCGCCAAGCACGCCCGGGCGAGTCGGATCCGGGTCGTGC
TGGAACGGGCGGGCGAGGGCGAGATCCGGTTGCTCGTGGCCGACGACGGCGTCGGATTCGACCGCCGGGCCGGCATCCGG
CCAACGGCCGTGGGCCTGGCGGGGATGCACGAGCGCATGGCCGCCCTCGGCGGGAGCATTCAGGTGCGCAGCGCCCCCGG
GGAAGGCACCGAGGTGCTGGCGGTCCTGCCGCCTTCGCCGCCGAGGGACGAGGGCGGCGGTCCATAG

Upstream 100 bases:

>100_bases
GTGCCGGGCGCGGATGCGGAACTGATCATCGACGTGCACGTCGGGGTCGGCGACATCACCATCGAGCGGGTGCCGACCAC
CCAGTTCCGGGTGTTCTGAC

Downstream 100 bases:

>100_bases
GGTGGCGGCGGTGCGGACAGGCAGCCTACCCGGCGCAGGGGGCCGGGCGGGCGTCGAAGAGGCGCAGGGTGAGCGGGCGC
CGCATGCGCAGCGGGGCGGC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEGRMEERRTLGLQWQLVAVTAAAGLLASGLAVFGVWVALQQGFGWNEALVAGVAAGVAGGLAAAAWSFQLARGIKRRLW
NAGDLAARIRRGDLSARLPVSDADEIGELEMQLNEMAAYLEQAVGQLSRLAEQNRLLAEEAGRGAALEERARIARDLHDT
VNQQLFVLSLRAAAVRKRVGAASGAAAGGDGAGVEPQLVTELANLEELARTAHSQIRELILQLRPTTLEQHGLGPALAEY
VKTVAERENWAVENEIDQALRLRGAEGEALFRVAQEALNNVAKHARASRIRVVLERAGEGEIRLLVADDGVGFDRRAGIR
PTAVGLAGMHERMAALGGSIQVRSAPGEGTEVLAVLPPSPPRDEGGGP

Sequences:

>Translated_368_residues
MEGRMEERRTLGLQWQLVAVTAAAGLLASGLAVFGVWVALQQGFGWNEALVAGVAAGVAGGLAAAAWSFQLARGIKRRLW
NAGDLAARIRRGDLSARLPVSDADEIGELEMQLNEMAAYLEQAVGQLSRLAEQNRLLAEEAGRGAALEERARIARDLHDT
VNQQLFVLSLRAAAVRKRVGAASGAAAGGDGAGVEPQLVTELANLEELARTAHSQIRELILQLRPTTLEQHGLGPALAEY
VKTVAERENWAVENEIDQALRLRGAEGEALFRVAQEALNNVAKHARASRIRVVLERAGEGEIRLLVADDGVGFDRRAGIR
PTAVGLAGMHERMAALGGSIQVRSAPGEGTEVLAVLPPSPPRDEGGGP
>Mature_368_residues
MEGRMEERRTLGLQWQLVAVTAAAGLLASGLAVFGVWVALQQGFGWNEALVAGVAAGVAGGLAAAAWSFQLARGIKRRLW
NAGDLAARIRRGDLSARLPVSDADEIGELEMQLNEMAAYLEQAVGQLSRLAEQNRLLAEEAGRGAALEERARIARDLHDT
VNQQLFVLSLRAAAVRKRVGAASGAAAGGDGAGVEPQLVTELANLEELARTAHSQIRELILQLRPTTLEQHGLGPALAEY
VKTVAERENWAVENEIDQALRLRGAEGEALFRVAQEALNNVAKHARASRIRVVLERAGEGEIRLLVADDGVGFDRRAGIR
PTAVGLAGMHERMAALGGSIQVRSAPGEGTEVLAVLPPSPPRDEGGGP

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=213, Percent_Identity=29.1079812206573, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788812, Length=208, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082311, Length=258, Percent_Identity=29.4573643410853, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39036; Mature: 39036

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGRMEERRTLGLQWQLVAVTAAAGLLASGLAVFGVWVALQQGFGWNEALVAGVAAGVAG
CCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLAAAAWSFQLARGIKRRLWNAGDLAARIRRGDLSARLPVSDADEIGELEMQLNEMAAYL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EQAVGQLSRLAEQNRLLAEEAGRGAALEERARIARDLHDTVNQQLFVLSLRAAAVRKRVG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AASGAAAGGDGAGVEPQLVTELANLEELARTAHSQIRELILQLRPTTLEQHGLGPALAEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHH
VKTVAERENWAVENEIDQALRLRGAEGEALFRVAQEALNNVAKHARASRIRVVLERAGEG
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EIRLLVADDGVGFDRRAGIRPTAVGLAGMHERMAALGGSIQVRSAPGEGTEVLAVLPPSP
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCC
PRDEGGGP
CCCCCCCC
>Mature Secondary Structure
MEGRMEERRTLGLQWQLVAVTAAAGLLASGLAVFGVWVALQQGFGWNEALVAGVAAGVAG
CCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLAAAAWSFQLARGIKRRLWNAGDLAARIRRGDLSARLPVSDADEIGELEMQLNEMAAYL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EQAVGQLSRLAEQNRLLAEEAGRGAALEERARIARDLHDTVNQQLFVLSLRAAAVRKRVG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AASGAAAGGDGAGVEPQLVTELANLEELARTAHSQIRELILQLRPTTLEQHGLGPALAEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHH
VKTVAERENWAVENEIDQALRLRGAEGEALFRVAQEALNNVAKHARASRIRVVLERAGEG
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EIRLLVADDGVGFDRRAGIRPTAVGLAGMHERMAALGGSIQVRSAPGEGTEVLAVLPPSP
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCC
PRDEGGGP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]