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The map label for this gene is liaR [H]

Identifier: 51893866

GI number: 51893866

Start: 2929890

End: 2930522

Strand: Reverse

Name: liaR [H]

Synonym: STH2728

Alternate gene names: 51893866

Gene position: 2930522-2929890 (Counterclockwise)

Preceding gene: 51893867

Following gene: 51893864

Centisome position: 82.18

GC content: 66.51

Gene sequence:

>633_bases
GTGATTCGGCTGTTGATCGTGGATGACCACAAGATGGTGCGGGAAGGGCTGAAGATCTACCTCTCCACCGAGCCGGACAT
CGTGGTGGTGGGGGAGGCGGAGAACGGCGAAGAGGCCGCGCGGCTGACGGCGGAGCTGAAGCCGGACGTGATCCTGATGG
ACCTGATCATGCCCCGGGTGGACGGCATCGAGGGGACGAAGCGGTGTCTGGAGGCCCATCCGGGCGTGCGGGTGATCGTC
CTGACGTCGATGCCCGACGACGAGCTGGTCGTCCCGGCGATCCGGGCGGGCGCCCTCTCCTACGTGCTGAAGGACATTGC
GGCCGATGAGCTGGCGGAGACCATCCGGAAGGCCGTTTCGGGCCAGCCTACGCTGCACCCGGTCGCCGCCCAGCGGATGA
TGCAGGAGCTCACCGCACCGCCCAAGGCCTCGGCGGGGGTGGAGGAGATCACGCCCCGGGAGCTGGAGGTCCTGAAGCTG
ATCGCCCAGGGGCTCTCCAACAAGGAGATCGGGGAGCGGCTCTTCATCGGGGAGCGGACGGTGAAGACGCACGTTTCGCA
CCTGCTGGAGAAGCTGCAGCTCCAGGACCGGACGCAGCTGGCGATTTACGCGCTGCAGAACAAGTTGGTGTAG

Upstream 100 bases:

>100_bases
GGGCGCCGCATGCGCAGCGGGGCGGCGTGCGCCGGGCGCCCAGGCGTTAACAGTGGTAGTGTCGCGGCAGTAGGTGCGGG
ACAATCAGGAGGTGGCCCGG

Downstream 100 bases:

>100_bases
TCCTGGGTGGAACCGTGCGGCCGCTCCCTGTTGCCGGCCGCCATCCCCTCATCGTTCTGGCCTTGTTCTGTCATATGTGT
AGTCCCCCGGGGCCACCGGC

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIRLLIVDDHKMVREGLKIYLSTEPDIVVVGEAENGEEAARLTAELKPDVILMDLIMPRVDGIEGTKRCLEAHPGVRVIV
LTSMPDDELVVPAIRAGALSYVLKDIAADELAETIRKAVSGQPTLHPVAAQRMMQELTAPPKASAGVEEITPRELEVLKL
IAQGLSNKEIGERLFIGERTVKTHVSHLLEKLQLQDRTQLAIYALQNKLV

Sequences:

>Translated_210_residues
MIRLLIVDDHKMVREGLKIYLSTEPDIVVVGEAENGEEAARLTAELKPDVILMDLIMPRVDGIEGTKRCLEAHPGVRVIV
LTSMPDDELVVPAIRAGALSYVLKDIAADELAETIRKAVSGQPTLHPVAAQRMMQELTAPPKASAGVEEITPRELEVLKL
IAQGLSNKEIGERLFIGERTVKTHVSHLLEKLQLQDRTQLAIYALQNKLV
>Mature_210_residues
MIRLLIVDDHKMVREGLKIYLSTEPDIVVVGEAENGEEAARLTAELKPDVILMDLIMPRVDGIEGTKRCLEAHPGVRVIV
LTSMPDDELVVPAIRAGALSYVLKDIAADELAETIRKAVSGQPTLHPVAAQRMMQELTAPPKASAGVEEITPRELEVLKL
IAQGLSNKEIGERLFIGERTVKTHVSHLLEKLQLQDRTQLAIYALQNKLV

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787473, Length=208, Percent_Identity=39.9038461538462, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=32.3809523809524, Blast_Score=116, Evalue=9e-28,
Organism=Escherichia coli, GI1788521, Length=212, Percent_Identity=36.7924528301887, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1790102, Length=202, Percent_Identity=32.1782178217822, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=27.1844660194175, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=27.3170731707317, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082052, Length=115, Percent_Identity=35.6521739130435, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788550, Length=120, Percent_Identity=36.6666666666667, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1789937, Length=209, Percent_Identity=25.3588516746411, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788546, Length=210, Percent_Identity=27.6190476190476, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23133; Mature: 23133

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLLIVDDHKMVREGLKIYLSTEPDIVVVGEAENGEEAARLTAELKPDVILMDLIMPRV
CEEEEEECCHHHHHHCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHC
DGIEGTKRCLEAHPGVRVIVLTSMPDDELVVPAIRAGALSYVLKDIAADELAETIRKAVS
CCCHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQPTLHPVAAQRMMQELTAPPKASAGVEEITPRELEVLKLIAQGLSNKEIGERLFIGERT
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHH
VKTHVSHLLEKLQLQDRTQLAIYALQNKLV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIRLLIVDDHKMVREGLKIYLSTEPDIVVVGEAENGEEAARLTAELKPDVILMDLIMPRV
CEEEEEECCHHHHHHCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHC
DGIEGTKRCLEAHPGVRVIVLTSMPDDELVVPAIRAGALSYVLKDIAADELAETIRKAVS
CCCHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQPTLHPVAAQRMMQELTAPPKASAGVEEITPRELEVLKLIAQGLSNKEIGERLFIGERT
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHH
VKTHVSHLLEKLQLQDRTQLAIYALQNKLV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]