Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 51599055

GI number: 51599055

Start: 849418

End: 850266

Strand: Direct

Name: truB [H]

Synonym: BG0829

Alternate gene names: 51599055

Gene position: 849418-850266 (Clockwise)

Preceding gene: 51599054

Following gene: 51599056

Centisome position: 93.94

GC content: 27.8

Gene sequence:

>849_bases
TTGGAAAATGGATTCCTTTTAATTAACAAAGAACAAGGCAAAACTTCTTTTGAAACTCTTTTTCCTATAAAAAAATATTT
TAATACAAATCATGTTGGGCATGCTGGTACACTTGATAAATTTGCGAGTGGAATTTTAATTGCTCTTGTAGGGAAATATA
CGAAGCTTGCAGGCTATTTTATTTCTTTGGATAAAGAGTATGTAGCAGAATTTAGATTTGGATTAGAAACAGATACTCTT
GATTCAAATGGAAGAATAGTTAATAAAGCGGATTATATCCCTAGTGTAGAGGATATAGATTTAAAGCTTAAAGACTTTGT
GGGTGAGATTTATCAAAGCCCTCCTAGATTTTCTTCTATTCATATTGATGGTATCAGGGCTTATAAACTTGCTTTAAATG
GAAAGTTTTTTGAAATTAAAAAACGAAGAGTAAATGTCTATGATATCCAAAGATTAAGCTACGATTTTAGTTCTTCTTCG
CTTAGTTTGAAAATTACTTGTTCAAAAGGTACTTATATTAGGAGTATTGCAAGAGATTTGGCTTATTCTTTAAATTCTTG
CGCATATGTTAGTAATTTAAAAAGAACCAAAATAGGTATGTTTAGATTAAAAGATTCTACATTGTGTAAAAATTTGAGTA
AATCTTCCTTAATTAGTTTAGAATCTTTAAGCAGTTTTAAAAAGGTTTATATTGATTCTAGTAAAGTAAATCTTGTTAAA
AATGGGGCTTACGTTGAAGTTCAAATCAATATTAATGAATTTAAGATTTTAAAATCCAGAGAAGGGGAAATATTAGCGGT
CATTGAAGGAATAGGTTTTAATAAATATAAATATGTTATTATATTTTAA

Upstream 100 bases:

>100_bases
GTACTCCTAAATTATTATTTGTTAAGGATGATTCTCTTTCTAAATCATTTTATGTTAATAAAATCATTGAAGGATTAAAT
ACTACAAGAGAGAATTAAAT

Downstream 100 bases:

>100_bases
TTTTATACTTTTTTTAAAAAATTTAATAATTTATTATTAAAAATAACCTAGAAAGGTAAATTGTTTTTGTGATTTTTAAG
AAAAAATATTATAATCTATC

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MENGFLLINKEQGKTSFETLFPIKKYFNTNHVGHAGTLDKFASGILIALVGKYTKLAGYFISLDKEYVAEFRFGLETDTL
DSNGRIVNKADYIPSVEDIDLKLKDFVGEIYQSPPRFSSIHIDGIRAYKLALNGKFFEIKKRRVNVYDIQRLSYDFSSSS
LSLKITCSKGTYIRSIARDLAYSLNSCAYVSNLKRTKIGMFRLKDSTLCKNLSKSSLISLESLSSFKKVYIDSSKVNLVK
NGAYVEVQININEFKILKSREGEILAVIEGIGFNKYKYVIIF

Sequences:

>Translated_282_residues
MENGFLLINKEQGKTSFETLFPIKKYFNTNHVGHAGTLDKFASGILIALVGKYTKLAGYFISLDKEYVAEFRFGLETDTL
DSNGRIVNKADYIPSVEDIDLKLKDFVGEIYQSPPRFSSIHIDGIRAYKLALNGKFFEIKKRRVNVYDIQRLSYDFSSSS
LSLKITCSKGTYIRSIARDLAYSLNSCAYVSNLKRTKIGMFRLKDSTLCKNLSKSSLISLESLSSFKKVYIDSSKVNLVK
NGAYVEVQININEFKILKSREGEILAVIEGIGFNKYKYVIIF
>Mature_282_residues
MENGFLLINKEQGKTSFETLFPIKKYFNTNHVGHAGTLDKFASGILIALVGKYTKLAGYFISLDKEYVAEFRFGLETDTL
DSNGRIVNKADYIPSVEDIDLKLKDFVGEIYQSPPRFSSIHIDGIRAYKLALNGKFFEIKKRRVNVYDIQRLSYDFSSSS
LSLKITCSKGTYIRSIARDLAYSLNSCAYVSNLKRTKIGMFRLKDSTLCKNLSKSSLISLESLSSFKKVYIDSSKVNLVK
NGAYVEVQININEFKILKSREGEILAVIEGIGFNKYKYVIIF

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=229, Percent_Identity=35.8078602620087, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI2367200, Length=212, Percent_Identity=33.9622641509434, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17553978, Length=203, Percent_Identity=25.1231527093596, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=34.6456692913386, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323204, Length=293, Percent_Identity=22.8668941979522, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281364189, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281364187, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281364185, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281364183, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI62471759, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17975520, Length=293, Percent_Identity=24.5733788395904, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 31967; Mature: 31967

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENGFLLINKEQGKTSFETLFPIKKYFNTNHVGHAGTLDKFASGILIALVGKYTKLAGYF
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEHHHHHHHHHEEE
ISLDKEYVAEFRFGLETDTLDSNGRIVNKADYIPSVEDIDLKLKDFVGEIYQSPPRFSSI
EEECHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCHHCCCHHHHHHHHHHCCCCCCCEE
HIDGIRAYKLALNGKFFEIKKRRVNVYDIQRLSYDFSSSSLSLKITCSKGTYIRSIARDL
EECCEEEEEEEECCEEEEEEECCCCEEEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHH
AYSLNSCAYVSNLKRTKIGMFRLKDSTLCKNLSKSSLISLESLSSFKKVYIDSSKVNLVK
HHHHCCHHHHHCCCHHEEEEEEECCHHHHHCCCHHHEEEHHHHHHHHHEEECCCCEEEEE
NGAYVEVQININEFKILKSREGEILAVIEGIGFNKYKYVIIF
CCEEEEEEEECCCEEEEECCCCCEEEEEECCCCCCEEEEEEC
>Mature Secondary Structure
MENGFLLINKEQGKTSFETLFPIKKYFNTNHVGHAGTLDKFASGILIALVGKYTKLAGYF
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEHHHHHHHHHEEE
ISLDKEYVAEFRFGLETDTLDSNGRIVNKADYIPSVEDIDLKLKDFVGEIYQSPPRFSSI
EEECHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCHHCCCHHHHHHHHHHCCCCCCCEE
HIDGIRAYKLALNGKFFEIKKRRVNVYDIQRLSYDFSSSSLSLKITCSKGTYIRSIARDL
EECCEEEEEEEECCEEEEEEECCCCEEEEEEEEECCCCCCEEEEEEECCCHHHHHHHHHH
AYSLNSCAYVSNLKRTKIGMFRLKDSTLCKNLSKSSLISLESLSSFKKVYIDSSKVNLVK
HHHHCCHHHHHCCCHHEEEEEEECCHHHHHCCCHHHEEEHHHHHHHHHEEECCCCEEEEE
NGAYVEVQININEFKILKSREGEILAVIEGIGFNKYKYVIIF
CCEEEEEEEECCCEEEEECCCCCEEEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA