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The map label for this gene is dus [H]

Identifier: 51598988

GI number: 51598988

Start: 779639

End: 780628

Strand: Direct

Name: dus [H]

Synonym: BG0758

Alternate gene names: 51598988

Gene position: 779639-780628 (Clockwise)

Preceding gene: 51598987

Following gene: 51598989

Centisome position: 86.22

GC content: 28.28

Gene sequence:

>990_bases
ATGAAGTTTTTATTTGATATTCCTCTTCCAATTATGATTTTGGCTCCAATGGAAGACGTTACTGATACTGTTTTTAGAAA
TTTAATTCATTTAATAGGATCTAAAGAAGGAGAACCCCATATTTATTTTACTGAATTTATTTCTGTAAAGGGAATTTTAA
ATGAGTCAAAACAGTCTATTCAACATGTTTTTTTAAAACCCAATGAACTTAATAGGCCTTTAATTGCTCAAATTTGGGGT
AATGATCCTGAGCAATTTTATAGAGCAATAGAAATATTAGGGGGCATGGGATTTTGGGGAATTGATATTAATATGGGTTG
TCCTAAGAATAAAATAATTAAAAAAGGGGTTTGCTCGGCTTTAATTAATAATAAATCTTTAGCCAAAGAAATAATTCTTG
CAAGCAAAGAAGCTTGTGCGAGATTTAATTTACCTCTTAGCGTTAAGACCAGGCATGGATTTTCATATCCAGAAGTTAAT
GATTGGCTGGGATTTTTATTGGGCTTTGGAATTGATATGTTAACGGTTTATCCAAGACTTGCTGTTAATCAGAGCGAAGG
CCCTGTTAATTTTGATATTTTTTATGAACTTGTTAAATTGCGGAATAATCTTAGTCCTTCTACTCTGATTATTGGCAATG
GAGATGTTTTAAGTCTTAAGGATGCTAGATATTACGTTGATAAATATTTAATTGATGGGATTATGTTTGGTCGTGGAATT
TTTAAGAATTTAAATTTATTTAAATTATCTTCAAAACATTTTTTGGATAGTAATTTAAATTTTAGATTAAATATATTAAA
ATTCCATATAAAGGATTTTCATGCTACTTGGGGACTTGAAAAAGATTTTAATAAACTTAAAAAATATTTTAAGATATATT
TTACTGAGAAGGAAAGACAGAGTAAATATTTCTCAAATCTTATAAATTCAAAAACTTACGAAGAGCTTTTTGAAAATTTA
AACGTTATTGACATGGTAGGAGATTTTTAA

Upstream 100 bases:

>100_bases
TTATTCTAAAAATTATAATGTTATTTTTAAAAATTTGAATCTATTAAGCAAAGCAACTGTTTTGATTAATAGATATGTTT
TATTGTTATATAGTTAATTG

Downstream 100 bases:

>100_bases
AACAATGAATTTTAGACTTCTTGAAAAATATGATCCCAAGGCATTTGAAGATGAAATTTACAACAAATGGCTTAAAAATA
ATGTTTTTTTGCCAAATAAT

Product: histidine phosphokinase/phophatase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSKEGEPHIYFTEFISVKGILNESKQSIQHVFLKPNELNRPLIAQIWG
NDPEQFYRAIEILGGMGFWGIDINMGCPKNKIIKKGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVN
DWLGFLLGFGIDMLTVYPRLAVNQSEGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKDARYYVDKYLIDGIMFGRGI
FKNLNLFKLSSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQSKYFSNLINSKTYEELFENL
NVIDMVGDF

Sequences:

>Translated_329_residues
MKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSKEGEPHIYFTEFISVKGILNESKQSIQHVFLKPNELNRPLIAQIWG
NDPEQFYRAIEILGGMGFWGIDINMGCPKNKIIKKGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVN
DWLGFLLGFGIDMLTVYPRLAVNQSEGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKDARYYVDKYLIDGIMFGRGI
FKNLNLFKLSSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQSKYFSNLINSKTYEELFENL
NVIDMVGDF
>Mature_329_residues
MKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSKEGEPHIYFTEFISVKGILNESKQSIQHVFLKPNELNRPLIAQIWG
NDPEQFYRAIEILGGMGFWGIDINMGCPKNKIIKKGVCSALINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVN
DWLGFLLGFGIDMLTVYPRLAVNQSEGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKDARYYVDKYLIDGIMFGRGI
FKNLNLFKLSSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQSKYFSNLINSKTYEELFENL
NVIDMVGDF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=262, Percent_Identity=24.4274809160305, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789660, Length=297, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI145693211, Length=258, Percent_Identity=23.2558139534884, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=207, Percent_Identity=27.0531400966184, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=239, Percent_Identity=26.3598326359833, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=250, Percent_Identity=26.8, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37980; Mature: 37980

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSKEGEPHIYFTEFISVKGILNESKQSI
CCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHH
QHVFLKPNELNRPLIAQIWGNDPEQFYRAIEILGGMGFWGIDINMGCPKNKIIKKGVCSA
HHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
LINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVNDWLGFLLGFGIDMLTVYPRL
HHCCHHHHHHHHHHCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
AVNQSEGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKDARYYVDKYLIDGIMFGRGI
EECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHH
FKNLNLFKLSSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQ
HCCCCEEEECCCHHCCCCCCEEEEEEEEEEHHHHHHCCCHHHHHHHHHHHHEEECCHHHH
SKYFSNLINSKTYEELFENLNVIDMVGDF
HHHHHHHHCCHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MKFLFDIPLPIMILAPMEDVTDTVFRNLIHLIGSKEGEPHIYFTEFISVKGILNESKQSI
CCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHH
QHVFLKPNELNRPLIAQIWGNDPEQFYRAIEILGGMGFWGIDINMGCPKNKIIKKGVCSA
HHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
LINNKSLAKEIILASKEACARFNLPLSVKTRHGFSYPEVNDWLGFLLGFGIDMLTVYPRL
HHCCHHHHHHHHHHCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
AVNQSEGPVNFDIFYELVKLRNNLSPSTLIIGNGDVLSLKDARYYVDKYLIDGIMFGRGI
EECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHH
FKNLNLFKLSSKHFLDSNLNFRLNILKFHIKDFHATWGLEKDFNKLKKYFKIYFTEKERQ
HCCCCEEEECCCHHCCCCCCEEEEEEEEEEHHHHHHCCCHHHHHHHHHHHHEEECCHHHH
SKYFSNLINSKTYEELFENLNVIDMVGDF
HHHHHHHHCCHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA