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The map label for this gene is pfk [H]

Identifier: 51598979

GI number: 51598979

Start: 767273

End: 768616

Strand: Direct

Name: pfk [H]

Synonym: BG0749

Alternate gene names: 51598979

Gene position: 767273-768616 (Clockwise)

Preceding gene: 51598978

Following gene: 51598985

Centisome position: 84.85

GC content: 34.15

Gene sequence:

>1344_bases
GTGTATAGAATTAAAAATGAAAATTTAGATTTTAAAATAGATAGTTTAGGGGAATGCAAGCAAAACAATCCTTTGATTGA
TTTTTATGCTGGTGAGGGCTCTTCACATTTTGTTAATGAAAAAAATAAAATTAAATTTAGTGTATATAGAAATGAGGACG
AGGGAGATAAGTATGAAGATATTCTTTTAGAAAAAGCTGGACCTAGAGAAAAAATTTATTTTGTGCCCAGGCACGTTAAA
GCTGCTATTACTACTTGCGGTGGACTTTGTCCTGGCTTTAACGATGTTATTCGTTCTATTGTGCGAACTTTATGGAAAAT
TTATGGGGTTCGTAATATTTATGGGGTAAAATTTGGATATCAAGGTCTTCTTCCTGAGTCAAATTCACCTTTTATTAATC
TTAATCCAGATGTTGGTGATGATATTAATAAATTTGGAGGCACTATTCTTGGCTCTTCAAGGGGTGGTATTAAACCCGTT
GAAATAGTTGACACTTTGGAGAGAATGAACATTAATATGATTTTTAATATTGGTGGAGATGGTACTCAAAAGGGGTCTCT
TCTTATTGCTGAGGAGATAGAAAAAAGAAATTTAAAAATAGCGGTTGTAGGCATTCCTAAAACTGTAGACAATGATTTTA
TGTTTGTTCAAAAATCTTTTGGATTTGAGACCGCTGTAGAACAAGCGGTTGCAGCTGTTGCTGGTGCTCATTTTGAAGCT
AATAGTGCTTATAATGGTATTGGACTTGTTAAAGTTATGGGGCGGGATTCTGGGTTTATTGCTGCTCATACTGCGCTTTC
TTCTAATGATGTTAATTTTTGTTTAATTCCAGAGCTTGATTTTGATATAGAAGGTCCTAATGGATTTCTTGTTCATCTTG
AAAGACGACTTTTAGAGAAAGAGAGCTTAGAGGAAATTCCTCATGCAGTAATATTGATAGCAGAAGGAGCAGGGCAAAAG
TATTTTAATCAGTTTCCTCGCAAAAAAGACGATTCTGGGAATTTGCTTTATGAGGATATTGGGCTTTATATTAAAGATAA
AATTACAGAATATTTTAAAGCAAAAAATATACAATTTACTCTTAAATACATTGATCCCAGCTATATTATTAGAAGTTCGC
CTGCTAATGCTAGTGATTCACTTTATTGTGCTAGGCTTGGTTCAAATGCTGTGCATGCTGCAATGTCTGGCAAGACAAAA
ATGTTGATTAGTTTGTGGAGTACAAAGTTTGTGCACATACCGATTAAGATGGCAGTAATTGACAGAAATAAGGTTAATCC
CAATGGTTCTTTTTGGAGAGATGTTCTTTCAAGCACAGGACAACCGATTAGCATGAAGAATTAA

Upstream 100 bases:

>100_bases
AGGGTCTCCTTATATTTACGACGACATATACGATATGATTAAAGATCAAAGTCAATTTATTAGAAAATAATTGGGTTATA
ATTCTATAAAGGAGTATTTA

Downstream 100 bases:

>100_bases
ATTAATTAAATTCTTTCTACTTAGCAGCATTTCCAGCAATATTTAATATATCCCAGGTTCTTGAGAATGGTGGAGAATAT
GAGAAGTCCATCATTCCTAG

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MYRIKNENLDFKIDSLGECKQNNPLIDFYAGEGSSHFVNEKNKIKFSVYRNEDEGDKYEDILLEKAGPREKIYFVPRHVK
AAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGYQGLLPESNSPFINLNPDVGDDINKFGGTILGSSRGGIKPV
EIVDTLERMNINMIFNIGGDGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFEA
NSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEKESLEEIPHAVILIAEGAGQK
YFNQFPRKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFTLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMSGKTK
MLISLWSTKFVHIPIKMAVIDRNKVNPNGSFWRDVLSSTGQPISMKN

Sequences:

>Translated_447_residues
MYRIKNENLDFKIDSLGECKQNNPLIDFYAGEGSSHFVNEKNKIKFSVYRNEDEGDKYEDILLEKAGPREKIYFVPRHVK
AAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGYQGLLPESNSPFINLNPDVGDDINKFGGTILGSSRGGIKPV
EIVDTLERMNINMIFNIGGDGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFEA
NSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEKESLEEIPHAVILIAEGAGQK
YFNQFPRKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFTLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMSGKTK
MLISLWSTKFVHIPIKMAVIDRNKVNPNGSFWRDVLSSTGQPISMKN
>Mature_447_residues
MYRIKNENLDFKIDSLGECKQNNPLIDFYAGEGSSHFVNEKNKIKFSVYRNEDEGDKYEDILLEKAGPREKIYFVPRHVK
AAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGYQGLLPESNSPFINLNPDVGDDINKFGGTILGSSRGGIKPV
EIVDTLERMNINMIFNIGGDGTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFEA
NSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEKESLEEIPHAVILIAEGAGQK
YFNQFPRKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFTLKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMSGKTK
MLISLWSTKFVHIPIKMAVIDRNKVNPNGSFWRDVLSSTGQPISMKN

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=377, Percent_Identity=25.1989389920424, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI266453768, Length=377, Percent_Identity=25.1989389920424, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI266453748, Length=377, Percent_Identity=25.1989389920424, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4505749, Length=377, Percent_Identity=25.1989389920424, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI11321601, Length=260, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790350, Length=212, Percent_Identity=31.6037735849057, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25147584, Length=372, Percent_Identity=25.2688172043011, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558788, Length=348, Percent_Identity=22.9885057471264, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17647809, Length=393, Percent_Identity=23.4096692111959, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652337, Length=393, Percent_Identity=23.4096692111959, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28573326, Length=393, Percent_Identity=23.6641221374046, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 49737; Mature: 49737

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIKNENLDFKIDSLGECKQNNPLIDFYAGEGSSHFVNEKNKIKFSVYRNEDEGDKYED
CCEECCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHH
ILLEKAGPREKIYFVPRHVKAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGY
HHCCCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
QGLLPESNSPFINLNPDVGDDINKFGGTILGSSRGGIKPVEIVDTLERMNINMIFNIGGD
CCCCCCCCCCEEEECCCCCCCHHHHCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCC
GTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFEA
CCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEC
NSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEK
CCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCEEECCCCEEEEEHHHHHHH
ESLEEIPHAVILIAEGAGQKYFNQFPRKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFT
HHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHHHHCCCEEEE
LKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMSGKTKMLISLWSTKFVHIPIKMAVI
EEEECCCEEEECCCCCCCCCEEEEECCCCCEEEHHCCCCEEEEEEECCEEEEEEEEEEEE
DRNKVNPNGSFWRDVLSSTGQPISMKN
ECCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYRIKNENLDFKIDSLGECKQNNPLIDFYAGEGSSHFVNEKNKIKFSVYRNEDEGDKYED
CCEECCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHH
ILLEKAGPREKIYFVPRHVKAAITTCGGLCPGFNDVIRSIVRTLWKIYGVRNIYGVKFGY
HHCCCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
QGLLPESNSPFINLNPDVGDDINKFGGTILGSSRGGIKPVEIVDTLERMNINMIFNIGGD
CCCCCCCCCCEEEECCCCCCCHHHHCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCC
GTQKGSLLIAEEIEKRNLKIAVVGIPKTVDNDFMFVQKSFGFETAVEQAVAAVAGAHFEA
CCCCCCEEEEEHHHHCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEC
NSAYNGIGLVKVMGRDSGFIAAHTALSSNDVNFCLIPELDFDIEGPNGFLVHLERRLLEK
CCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCEEECCCCEEEEEHHHHHHH
ESLEEIPHAVILIAEGAGQKYFNQFPRKKDDSGNLLYEDIGLYIKDKITEYFKAKNIQFT
HHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHHHHHCCCEEEE
LKYIDPSYIIRSSPANASDSLYCARLGSNAVHAAMSGKTKMLISLWSTKFVHIPIKMAVI
EEEECCCEEEECCCCCCCCCEEEEECCCCCEEEHHCCCCEEEEEEECCEEEEEEEEEEEE
DRNKVNPNGSFWRDVLSSTGQPISMKN
ECCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]