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The map label for this gene is rpoD [H]

Identifier: 51598964

GI number: 51598964

Start: 750021

End: 751916

Strand: Direct

Name: rpoD [H]

Synonym: BG0734

Alternate gene names: 51598964

Gene position: 750021-751916 (Clockwise)

Preceding gene: 51598963

Following gene: 51598965

Centisome position: 82.94

GC content: 31.12

Gene sequence:

>1896_bases
TTGTCCGATTTGGAAAAGAAATATTCGAAGCTTATAGAGGGTATTGTTACTCATTTGGGAGATAGAAAATCTCTTAGTTT
TAGTGAATTGTCAAATTTACTTCCCGATGATATATTAGAGCCAGAGATACTTGATTTTATTTGTTCGATACTTGAGGATA
GGGGAATAATATTAGTTAATAAAATTTCAGAATTGGATCTGGTCGGCACCGAAGATGGAAATGATGAAGAGGAAGAGTCA
GAGATTGAATCTGATAGAAATTTTATGATTTTAGATGATGGTTTTCAAAGTGATGAGGAAGATATTGATATTGATGGAAA
GCTGGATGATTGTGATGAAGAAGATATTTCTGTAAAGGATGATTTAGGTTCAGGGTATATTAAAAGTAATGCCTTAAAAG
ATAGTCACTCAGAAGATCCAATAAAGCTTTATTTAAAGGAAATAGGAAAAGAGTTTTTATTAACAGGAAATCAGGAAGTT
GAACTTGCAAAGCAAATGGATTCTGGAGAGAGTATAATTGAGAATATTCTTAAGAATGAGGGACTTGTTATAGAAAATTA
TTATAATCTTATTAATACTATTTATTCAAGAATGGAAAGGGAAGAGTTTTTTAAAAGAGAAAAAGATAAGGATAAAGAGA
GTAACCCTGATTATTATAATAAAAAAAAAAGAATTGCCTCTTTTTATAAAATTCCTTTAAAACCAATTCAAGATCGTTTA
ATAAGTTATGTAGATAATAAGCATAGGGTATATGAGCTTGGGGGGGATATTTTTGAAAAGAATTTAAAAAGTGAGAGATT
GGCCCTAAAAGAGCTTTTAAGAGATGTTCCTTTATATCAAGATGAACTAAGGATTTTTTCAGATGATTATATTGACTCTG
CTAACAAAATAAAGGATTTGCAAAGACAGCAAAGAATAATTTTAAGCAGGTTGAAAATTGAAAAAATAAGAGACTTAAGG
GTACTTGGAAGAGATTTGACTATTGCTGAAAAAAGAATAGAGATAGAAAAATCTCTTAAACTTAAAGAAGATGCTATCAA
AGAGCAGATTACAGAGGCTCAACTTGCTCAGAAAGAACTTGAGAGAATTGAAATGTATTATGAATATCCAATGGATAAAA
TAATAAGCATGTCAGAAGAAATTGCTAAAGGAAAACAAATGATGCAGCATGCTAAAGATCAGTTGATTAAGGCCAATTTA
AGGCTTGTTGTAAGCATTGCTAAAAAATATGCAAATAGAGGTCTTCATTTTTTTGATCTTGTTCAAGAAGGCAATATTGG
ATTAATTAAAGCTGTTGAAAAGTTTGAATATAAGAGAGGTTTTAAATTTTCAACTTATGCTACCTGGTGGATTAGACAGG
CGATAACAAGATCTATTTCTGATCAAGCTCGCACAATTAGAGTTCCTGTCCATATGATTGAACAAATAAATAGGCTTAAT
AGAGAAACTAGATATTTAGTTCAAGTTTTAGGGAAAGATCCTACAGATGAAGAGCTCTCAGACAGACTTGGATGGGAGCT
TAAAAAGGTTAAAACTGTAAAAAGTGTTTCAAGGGAGCCTGTTTCTCTTGAAACGCCAATTGGAGAAGAAGAAGATTCTG
TTCTTAGTGATTTTATTGAGGATAAGGCAATAAAAAATCCTGCGAATCATACATCCTTTGTGGTTTTGCAAGATCAAATA
AGATCAATTCTTGGGACTCTTCCCGAAAGAGAGCAAGAAGTTGTAAAAATGAGATTTGGACTTGAAGATGGTTATTCTTT
AACTCTTGAAGAGGTTGGACTTCATTTTAATGTTACAAGAGAAAGAATTAGGCAAATTGAATCTAAGGCATTAAGGCGAC
TTAAAAATCCTAAAAAAACTCAAAAATTAAAAGATTATCTTGAAGATTTAAATTGA

Upstream 100 bases:

>100_bases
TTTGGTAAATGCTAAGATTCAAATAAATGAATTAATGTTTTTAAATATGCAGAGAAAAAATTTAAAAATCTACACAGACG
ATGTTCCAGGGAGTTAAGTT

Downstream 100 bases:

>100_bases
ACATGGAGGATTAATGGAGAATAATATTGATACATTAAAGAAACTTGAAATTATATATAAATCTAAGTTTGAGCTTGAGG
AAAGGCGAAAAAGTATTCCT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSDLEKKYSKLIEGIVTHLGDRKSLSFSELSNLLPDDILEPEILDFICSILEDRGIILVNKISELDLVGTEDGNDEEEES
EIESDRNFMILDDGFQSDEEDIDIDGKLDDCDEEDISVKDDLGSGYIKSNALKDSHSEDPIKLYLKEIGKEFLLTGNQEV
ELAKQMDSGESIIENILKNEGLVIENYYNLINTIYSRMEREEFFKREKDKDKESNPDYYNKKKRIASFYKIPLKPIQDRL
ISYVDNKHRVYELGGDIFEKNLKSERLALKELLRDVPLYQDELRIFSDDYIDSANKIKDLQRQQRIILSRLKIEKIRDLR
VLGRDLTIAEKRIEIEKSLKLKEDAIKEQITEAQLAQKELERIEMYYEYPMDKIISMSEEIAKGKQMMQHAKDQLIKANL
RLVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
RETRYLVQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPANHTSFVVLQDQI
RSILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN

Sequences:

>Translated_631_residues
MSDLEKKYSKLIEGIVTHLGDRKSLSFSELSNLLPDDILEPEILDFICSILEDRGIILVNKISELDLVGTEDGNDEEEES
EIESDRNFMILDDGFQSDEEDIDIDGKLDDCDEEDISVKDDLGSGYIKSNALKDSHSEDPIKLYLKEIGKEFLLTGNQEV
ELAKQMDSGESIIENILKNEGLVIENYYNLINTIYSRMEREEFFKREKDKDKESNPDYYNKKKRIASFYKIPLKPIQDRL
ISYVDNKHRVYELGGDIFEKNLKSERLALKELLRDVPLYQDELRIFSDDYIDSANKIKDLQRQQRIILSRLKIEKIRDLR
VLGRDLTIAEKRIEIEKSLKLKEDAIKEQITEAQLAQKELERIEMYYEYPMDKIISMSEEIAKGKQMMQHAKDQLIKANL
RLVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
RETRYLVQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPANHTSFVVLQDQI
RSILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN
>Mature_630_residues
SDLEKKYSKLIEGIVTHLGDRKSLSFSELSNLLPDDILEPEILDFICSILEDRGIILVNKISELDLVGTEDGNDEEEESE
IESDRNFMILDDGFQSDEEDIDIDGKLDDCDEEDISVKDDLGSGYIKSNALKDSHSEDPIKLYLKEIGKEFLLTGNQEVE
LAKQMDSGESIIENILKNEGLVIENYYNLINTIYSRMEREEFFKREKDKDKESNPDYYNKKKRIASFYKIPLKPIQDRLI
SYVDNKHRVYELGGDIFEKNLKSERLALKELLRDVPLYQDELRIFSDDYIDSANKIKDLQRQQRIILSRLKIEKIRDLRV
LGRDLTIAEKRIEIEKSLKLKEDAIKEQITEAQLAQKELERIEMYYEYPMDKIISMSEEIAKGKQMMQHAKDQLIKANLR
LVVSIAKKYANRGLHFFDLVQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLNR
ETRYLVQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIEDKAIKNPANHTSFVVLQDQIR
SILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTRERIRQIESKALRRLKNPKKTQKLKDYLEDLN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=278, Percent_Identity=51.7985611510791, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789098, Length=284, Percent_Identity=37.3239436619718, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789871, Length=267, Percent_Identity=28.4644194756554, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788231, Length=210, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 73611; Mature: 73480

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLEKKYSKLIEGIVTHLGDRKSLSFSELSNLLPDDILEPEILDFICSILEDRGIILVN
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEE
KISELDLVGTEDGNDEEEESEIESDRNFMILDDGFQSDEEDIDIDGKLDDCDEEDISVKD
CCCCCEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
DLGSGYIKSNALKDSHSEDPIKLYLKEIGKEFLLTGNQEVELAKQMDSGESIIENILKNE
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCC
GLVIENYYNLINTIYSRMEREEFFKREKDKDKESNPDYYNKKKRIASFYKIPLKPIQDRL
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
ISYVDNKHRVYELGGDIFEKNLKSERLALKELLRDVPLYQDELRIFSDDYIDSANKIKDL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
QRQQRIILSRLKIEKIRDLRVLGRDLTIAEKRIEIEKSLKLKEDAIKEQITEAQLAQKEL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIEMYYEYPMDKIISMSEEIAKGKQMMQHAKDQLIKANLRLVVSIAKKYANRGLHFFDL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
HHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RETRYLVQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIE
HHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
DKAIKNPANHTSFVVLQDQIRSILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTR
HHHHCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHH
ERIRQIESKALRRLKNPKKTQKLKDYLEDLN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLEKKYSKLIEGIVTHLGDRKSLSFSELSNLLPDDILEPEILDFICSILEDRGIILVN
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEE
KISELDLVGTEDGNDEEEESEIESDRNFMILDDGFQSDEEDIDIDGKLDDCDEEDISVKD
CCCCCEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
DLGSGYIKSNALKDSHSEDPIKLYLKEIGKEFLLTGNQEVELAKQMDSGESIIENILKNE
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCC
GLVIENYYNLINTIYSRMEREEFFKREKDKDKESNPDYYNKKKRIASFYKIPLKPIQDRL
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
ISYVDNKHRVYELGGDIFEKNLKSERLALKELLRDVPLYQDELRIFSDDYIDSANKIKDL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHH
QRQQRIILSRLKIEKIRDLRVLGRDLTIAEKRIEIEKSLKLKEDAIKEQITEAQLAQKEL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERIEMYYEYPMDKIISMSEEIAKGKQMMQHAKDQLIKANLRLVVSIAKKYANRGLHFFDL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VQEGNIGLIKAVEKFEYKRGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINRLN
HHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RETRYLVQVLGKDPTDEELSDRLGWELKKVKTVKSVSREPVSLETPIGEEEDSVLSDFIE
HHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
DKAIKNPANHTSFVVLQDQIRSILGTLPEREQEVVKMRFGLEDGYSLTLEEVGLHFNVTR
HHHHCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHH
ERIRQIESKALRRLKNPKKTQKLKDYLEDLN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 9084142 [H]