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The map label for this gene is dnaG [H]

Identifier: 51598963

GI number: 51598963

Start: 748236

End: 750017

Strand: Direct

Name: dnaG [H]

Synonym: BG0733

Alternate gene names: 51598963

Gene position: 748236-750017 (Clockwise)

Preceding gene: 51598962

Following gene: 51598964

Centisome position: 82.75

GC content: 26.94

Gene sequence:

>1782_bases
ATGGAGTATTTACAAACTGCAGCTTCAATTAAAGCTAAATTTGATATTGTAGCTATTGTGGAGCAATATATTAAGCTTGC
TAAATCGGGATCTACTTATAAAGGGCTGTGTCCTTTTCATGCCGAGAAAACCCCTTCTTTTTTTGTAAATTCTTTGCAAG
GATATTTTTATTGTTTTGGATGTAAAAAGGGTGGGGATGTTATTGGATTTTTAATGGATATGGAAAAAATCAATTACAAT
GATGCTCTTAAGATTTTATGTGAAAAATTTGGTATTTATTATAATGATTTAAAAATAAATCGAAAAAGTGAAAAAAAAAG
TGAAAATAAAGACATGGTTTCAAAAATTTACTCTTTAAACTCTAGATTAATCAATACTATTAAATTTTTTTTGAATAAAA
ATAAAAAAGCATTAGATTACGTTTTAAAGAGTAGAGCAATATCTAAGGAAGTCGTTGATTTATTTGAACTTGGTTATTTG
CCGTTTAGTATTAAAGATGGCTTAGAACTTCATGATTTTTTAGTTTCAAAAGGATACTCTTCTGAAATACTAAGGAAAAG
TGGTTTATTCTCAAAAACAAATCCCAAAGCTTCTATTTTATCTCAAAGATTAATTTTTCCAATTAAAGATTTTAAAGGAA
ATGTTGTTGGCTTTGGGGGCCGAGATTTAGATGGGAAAGGCTCTAAGTATATTAATTTAAGTGAAACTGAAGTTTTTAAA
AAAAAGGAGCTTCTTTATGGATTTTATGAGGGTCTTAATGAGATTAAATCTACAAAGTCAGTTATATTAGTAGAAGGATA
TATAGATGTTCTTGCCTTTTTTACATCTGGGATTAAGAGAGCTGTATCTACCCTTGGCACTTCTTTTTCAAAAGAACATC
TGGCTTTGATTCAAAGATATGCCAATGAGGTAATATTTTCTTTTGACGGGGATGATGCTGGACTTTCTGCAACTTTAAAG
GCTTATCAAATTTGTTTGCCATTTAATATTAATGTTAGTGTTGTTAAAATGGATTTTGGCCTTGATCCTGCGGATCTTCT
TAAAGATAAAGGTGAGGGATCTTTACAAAAAATTTTAAATAATAAATGCGATGCTTTTGAATATCTCTTAGATGCTTATT
CTAATAAATATGATTTAAATAAAACTGTAGATTTAAATGCTATGATTAATTTATTTTTAAATTTGATAAATTTATCTAAA
ATAGATACTCAAAAAAAAATTTTTTTAGATAAGCTAAGCAATAAGCTTGGTGTTGGTGTAACAACTTTATTGAAAGATTA
TTATAGAATAAAAGAAAGATTTGTAGTTGACAATAATAAAAGAAATTTGTATGCTCATAATGATGATTCTTATGAGAGGT
ATCTAATAGTAGCTTTGTTGAAAAATTTTAGTTATTTTGGTATAATAAGGCGCAATATTATTGATAGTGATTTAAGTGAT
ATCAATGCCAGAAAAATTTTTATGTGTTTTGAGGATTTATTTGAAAATAATAAAGATTTTTCATTAATGGATTTAAAAAG
AAATTTAAAAGATAGCTATAAAGTTAGTGAATTTTTTTTTGAAGAAATTTTAAATTCTGGATTTGAAATAGATGATGAGG
CGCTCATTCATATTTTAATTGCAATCAAGAGAAGAAAATTAGATTCTCGTGTTTTGCTTTGCAAGAAAAAAATTGATGGG
GACTCTTTGGTAAATGCTAAGATTCAAATAAATGAATTAATGTTTTTAAATATGCAGAGAAAAAATTTAAAAATCTACAC
AGACGATGTTCCAGGGAGTTAA

Upstream 100 bases:

>100_bases
ACTCTAAGTTGGGCACACATCAATTTTCATCAGATTATTCTTCACATCTTTTAGGAGCAAAAGATTATATTAAAAATTTT
ATTACTAAGGATTAAGAAGT

Downstream 100 bases:

>100_bases
GTTTTGTCCGATTTGGAAAAGAAATATTCGAAGCTTATAGAGGGTATTGTTACTCATTTGGGAGATAGAAAATCTCTTAG
TTTTAGTGAATTGTCAAATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MEYLQTAASIKAKFDIVAIVEQYIKLAKSGSTYKGLCPFHAEKTPSFFVNSLQGYFYCFGCKKGGDVIGFLMDMEKINYN
DALKILCEKFGIYYNDLKINRKSEKKSENKDMVSKIYSLNSRLINTIKFFLNKNKKALDYVLKSRAISKEVVDLFELGYL
PFSIKDGLELHDFLVSKGYSSEILRKSGLFSKTNPKASILSQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLSETEVFK
KKELLYGFYEGLNEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTSFSKEHLALIQRYANEVIFSFDGDDAGLSATLK
AYQICLPFNINVSVVKMDFGLDPADLLKDKGEGSLQKILNNKCDAFEYLLDAYSNKYDLNKTVDLNAMINLFLNLINLSK
IDTQKKIFLDKLSNKLGVGVTTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFGIIRRNIIDSDLSD
INARKIFMCFEDLFENNKDFSLMDLKRNLKDSYKVSEFFFEEILNSGFEIDDEALIHILIAIKRRKLDSRVLLCKKKIDG
DSLVNAKIQINELMFLNMQRKNLKIYTDDVPGS

Sequences:

>Translated_593_residues
MEYLQTAASIKAKFDIVAIVEQYIKLAKSGSTYKGLCPFHAEKTPSFFVNSLQGYFYCFGCKKGGDVIGFLMDMEKINYN
DALKILCEKFGIYYNDLKINRKSEKKSENKDMVSKIYSLNSRLINTIKFFLNKNKKALDYVLKSRAISKEVVDLFELGYL
PFSIKDGLELHDFLVSKGYSSEILRKSGLFSKTNPKASILSQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLSETEVFK
KKELLYGFYEGLNEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTSFSKEHLALIQRYANEVIFSFDGDDAGLSATLK
AYQICLPFNINVSVVKMDFGLDPADLLKDKGEGSLQKILNNKCDAFEYLLDAYSNKYDLNKTVDLNAMINLFLNLINLSK
IDTQKKIFLDKLSNKLGVGVTTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFGIIRRNIIDSDLSD
INARKIFMCFEDLFENNKDFSLMDLKRNLKDSYKVSEFFFEEILNSGFEIDDEALIHILIAIKRRKLDSRVLLCKKKIDG
DSLVNAKIQINELMFLNMQRKNLKIYTDDVPGS
>Mature_593_residues
MEYLQTAASIKAKFDIVAIVEQYIKLAKSGSTYKGLCPFHAEKTPSFFVNSLQGYFYCFGCKKGGDVIGFLMDMEKINYN
DALKILCEKFGIYYNDLKINRKSEKKSENKDMVSKIYSLNSRLINTIKFFLNKNKKALDYVLKSRAISKEVVDLFELGYL
PFSIKDGLELHDFLVSKGYSSEILRKSGLFSKTNPKASILSQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLSETEVFK
KKELLYGFYEGLNEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTSFSKEHLALIQRYANEVIFSFDGDDAGLSATLK
AYQICLPFNINVSVVKMDFGLDPADLLKDKGEGSLQKILNNKCDAFEYLLDAYSNKYDLNKTVDLNAMINLFLNLINLSK
IDTQKKIFLDKLSNKLGVGVTTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFGIIRRNIIDSDLSD
INARKIFMCFEDLFENNKDFSLMDLKRNLKDSYKVSEFFFEEILNSGFEIDDEALIHILIAIKRRKLDSRVLLCKKKIDG
DSLVNAKIQINELMFLNMQRKNLKIYTDDVPGS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=415, Percent_Identity=30.1204819277108, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 67868; Mature: 67868

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYLQTAASIKAKFDIVAIVEQYIKLAKSGSTYKGLCPFHAEKTPSFFVNSLQGYFYCFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEE
CKKGGDVIGFLMDMEKINYNDALKILCEKFGIYYNDLKINRKSEKKSENKDMVSKIYSLN
CCCCCCEEEHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECCCHHHCCCHHHHHHHHHHHH
SRLINTIKFFLNKNKKALDYVLKSRAISKEVVDLFELGYLPFSIKDGLELHDFLVSKGYS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCH
SEILRKSGLFSKTNPKASILSQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLSETEVFK
HHHHHHCCCCCCCCCHHHHHHHHHCEEECCCCCCEEECCCCCCCCCCCEEEECCHHHHHH
KKELLYGFYEGLNEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTSFSKEHLALIQRY
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ANEVIFSFDGDDAGLSATLKAYQICLPFNINVSVVKMDFGLDPADLLKDKGEGSLQKILN
HHHEEEEECCCCCCHHHEEEEEEEEEEECCCEEEEEEECCCCHHHHHHCCCCCHHHHHHC
NKCDAFEYLLDAYSNKYDLNKTVDLNAMINLFLNLINLSKIDTQKKIFLDKLSNKLGVGV
CCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCH
TTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFGIIRRNIIDSDLSD
HHHHHHHHHHHHHEEEECCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
INARKIFMCFEDLFENNKDFSLMDLKRNLKDSYKVSEFFFEEILNSGFEIDDEALIHILI
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AIKRRKLDSRVLLCKKKIDGDSLVNAKIQINELMFLNMQRKNLKIYTDDVPGS
HHHHHCCCCHHEEHHHCCCCCCEEEEEEEEEEEEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure
MEYLQTAASIKAKFDIVAIVEQYIKLAKSGSTYKGLCPFHAEKTPSFFVNSLQGYFYCFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEE
CKKGGDVIGFLMDMEKINYNDALKILCEKFGIYYNDLKINRKSEKKSENKDMVSKIYSLN
CCCCCCEEEHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECCCHHHCCCHHHHHHHHHHHH
SRLINTIKFFLNKNKKALDYVLKSRAISKEVVDLFELGYLPFSIKDGLELHDFLVSKGYS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCH
SEILRKSGLFSKTNPKASILSQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLSETEVFK
HHHHHHCCCCCCCCCHHHHHHHHHCEEECCCCCCEEECCCCCCCCCCCEEEECCHHHHHH
KKELLYGFYEGLNEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTSFSKEHLALIQRY
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ANEVIFSFDGDDAGLSATLKAYQICLPFNINVSVVKMDFGLDPADLLKDKGEGSLQKILN
HHHEEEEECCCCCCHHHEEEEEEEEEEECCCEEEEEEECCCCHHHHHHCCCCCHHHHHHC
NKCDAFEYLLDAYSNKYDLNKTVDLNAMINLFLNLINLSKIDTQKKIFLDKLSNKLGVGV
CCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCH
TTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFGIIRRNIIDSDLSD
HHHHHHHHHHHHHEEEECCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
INARKIFMCFEDLFENNKDFSLMDLKRNLKDSYKVSEFFFEEILNSGFEIDDEALIHILI
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AIKRRKLDSRVLLCKKKIDGDSLVNAKIQINELMFLNMQRKNLKIYTDDVPGS
HHHHHCCCCHHEEHHHCCCCCCEEEEEEEEEEEEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]