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The map label for this gene is fruK [H]

Identifier: 51598884

GI number: 51598884

Start: 663536

End: 664459

Strand: Direct

Name: fruK [H]

Synonym: BG0651

Alternate gene names: 51598884

Gene position: 663536-664459 (Clockwise)

Preceding gene: 51598882

Following gene: 51598890

Centisome position: 73.38

GC content: 29.98

Gene sequence:

>924_bases
TTGATATATACTCTAACACTTAATCCTTCTGTGGATTATAAAATAGTTTTAAAAGAATTTCAGGAAGAAAGTCTTAATTA
TGCTTTGAATAACAATTTCTTTGCTGGTGGTAAGGGGATAAATGTAAGCACTGTTCTTAAAAATTTAGGAAAGTCCAGTA
CGGCGCTTGGATTTTTAGGGGGTTTTACAGGTGATTATATTAAATCTTCTCTTGATTCTAAGGGTATAAAAAACGATTTT
ATTAAAATAAAATATGATACAAGATTAAATATTAAAATGATAGCAAATGGCAGAGAAACAGAAATTAATGCCAATTCTCC
AGATATTTTTGAAAATGAATTTAAACTTTTGAAAGATAAGCTTAAAAGTTTAACAAATAATAGTATATTGGTGATGTCGG
GAAGCGTTCCTGCAGCTCTTGGTGAGAATGCATACAATGAAATATCTAACACCATTTCTAATGATGTTAAGCTTATAATT
GATACTAGTGGTAAACCTTTAAAAAAAATTCTTAGATTAAATCCCTTTTTAATAAAACCCAATATTTATGAACTTGAGGA
TCTTTTTAATGCCAAATTAAATTCTACAAAAGAACTAATTAAAATTGGGAAAAATATTGTAGAAAGTGGAGTTCAAAATA
TTATAATTTCCATGGGAAGTGACGGAGCTATTTTTATTGGTAGTAAAAATGTTGCTTTTAGAGCCTTTGTTCCAAAGATC
AGCTCTGCTAGCACTATTGGAGCAGGAGACTCTGTGATTGCAGGATTTGTGTATGCTTTTGATAATAGAAATACTTTGAA
AGACTCATTTAAATTTGGTGTTGCGGCTGGTACAGCTACGGCATTAAAAGGTAGTCTTTGTGAATTTCAAGATGTTGAAA
AAATGCTTTGTGAGATTGGGATTGAGGATATTTGTATTTCTTAA

Upstream 100 bases:

>100_bases
AAATTTTGCATAATAATCACCTTTATATATTATATAGTGTATATATATTTATTAAATTTTACATTTATTTAATTAATTTT
ATCAATTAAAGGAGATTATC

Downstream 100 bases:

>100_bases
TCTTTTAAATCGAGCTGAAGATTTGATTTTGAAACTTTACTGTAGCTTTTTAAAAGCTACAGTATTTAGTTTGCTTAAAT
TTTTAATTTTGACTTATATA

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKSSTALGFLGGFTGDYIKSSLDSKGIKNDF
IKIKYDTRLNIKMIANGRETEINANSPDIFENEFKLLKDKLKSLTNNSILVMSGSVPAALGENAYNEISNTISNDVKLII
DTSGKPLKKILRLNPFLIKPNIYELEDLFNAKLNSTKELIKIGKNIVESGVQNIIISMGSDGAIFIGSKNVAFRAFVPKI
SSASTIGAGDSVIAGFVYAFDNRNTLKDSFKFGVAAGTATALKGSLCEFQDVEKMLCEIGIEDICIS

Sequences:

>Translated_307_residues
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKSSTALGFLGGFTGDYIKSSLDSKGIKNDF
IKIKYDTRLNIKMIANGRETEINANSPDIFENEFKLLKDKLKSLTNNSILVMSGSVPAALGENAYNEISNTISNDVKLII
DTSGKPLKKILRLNPFLIKPNIYELEDLFNAKLNSTKELIKIGKNIVESGVQNIIISMGSDGAIFIGSKNVAFRAFVPKI
SSASTIGAGDSVIAGFVYAFDNRNTLKDSFKFGVAAGTATALKGSLCEFQDVEKMLCEIGIEDICIS
>Mature_307_residues
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKSSTALGFLGGFTGDYIKSSLDSKGIKNDF
IKIKYDTRLNIKMIANGRETEINANSPDIFENEFKLLKDKLKSLTNNSILVMSGSVPAALGENAYNEISNTISNDVKLII
DTSGKPLKKILRLNPFLIKPNIYELEDLFNAKLNSTKELIKIGKNIVESGVQNIIISMGSDGAIFIGSKNVAFRAFVPKI
SSASTIGAGDSVIAGFVYAFDNRNTLKDSFKFGVAAGTATALKGSLCEFQDVEKMLCEIGIEDICIS

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=301, Percent_Identity=32.5581395348837, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788493, Length=273, Percent_Identity=30.03663003663, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33392; Mature: 33392

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKSSTALGFLG
CEEEEEECCCCCHHHHHHHHHHHHCCEEECCCEEECCCCCCHHHHHHHCCCCCCHHHHHC
GFTGDYIKSSLDSKGIKNDFIKIKYDTRLNIKMIANGRETEINANSPDIFENEFKLLKDK
CCCHHHHHHHHHCCCCCCCEEEEEECCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHH
LKSLTNNSILVMSGSVPAALGENAYNEISNTISNDVKLIIDTSGKPLKKILRLNPFLIKP
HHHHCCCEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEECC
NIYELEDLFNAKLNSTKELIKIGKNIVESGVQNIIISMGSDGAIFIGSKNVAFRAFVPKI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCEEEEECCCC
SSASTIGAGDSVIAGFVYAFDNRNTLKDSFKFGVAAGTATALKGSLCEFQDVEKMLCEIG
CCCCCCCCCCHHHEEEEEEECCCCHHHHHHHHCEECCHHHHHHCCCCCHHHHHHHHHHCC
IEDICIS
HHHHCCC
>Mature Secondary Structure
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKSSTALGFLG
CEEEEEECCCCCHHHHHHHHHHHHCCEEECCCEEECCCCCCHHHHHHHCCCCCCHHHHHC
GFTGDYIKSSLDSKGIKNDFIKIKYDTRLNIKMIANGRETEINANSPDIFENEFKLLKDK
CCCHHHHHHHHHCCCCCCCEEEEEECCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHH
LKSLTNNSILVMSGSVPAALGENAYNEISNTISNDVKLIIDTSGKPLKKILRLNPFLIKP
HHHHCCCEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEECC
NIYELEDLFNAKLNSTKELIKIGKNIVESGVQNIIISMGSDGAIFIGSKNVAFRAFVPKI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCEEEEECCCC
SSASTIGAGDSVIAGFVYAFDNRNTLKDSFKFGVAAGTATALKGSLCEFQDVEKMLCEIG
CCCCCCCCCCHHHEEEEEEECCCCHHHHHHHHCEECCHHHHHHCCCCCHHHHHHHHHHCC
IEDICIS
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]