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The map label for this gene is ptsI [H]

Identifier: 51598809

GI number: 51598809

Start: 571535

End: 573256

Strand: Direct

Name: ptsI [H]

Synonym: BG0568

Alternate gene names: 51598809

Gene position: 571535-573256 (Clockwise)

Preceding gene: 51598808

Following gene: 51598810

Centisome position: 63.21

GC content: 32.64

Gene sequence:

>1722_bases
ATGACTTTATCGGGTAAAAGAATATCCAAAGGAATAGGCATTGGAGAAGTTCTTTGTATTAGAAAAAATTTTGATAAAAT
TGTAAGTAGAGAAAAAATCGAATTTTCTCAAGTTGATAGCGAGATATCAAAATTCAATAAAGCTAAGTTAAAAGCAATTG
AAGCGCTTAAAGACCTTGATAGAAAAGCTGTGCTTCAATTTGGAGATGACAAGAAAGGTATTTTTGAAGGTCAAGTGTTG
ATCGTTGAAGACGATGAGCTTTCCGAGCTTGTTATTGAGCTTATTACAAAGGAAAATTATGGCGCTGCTTATTCTATTTA
TTTAGCGTTTGAAAATTTGGTTAAAAGCGTGGAAGACTATAAAGATCCTTATTTAAAAGAAAGAGCGTCTGATTATAAGG
ACATTAGGAATAGATTAATTTCTATCATTTTAGGTCAAGTAACCGATTTTTCTGAAATTAATAAAGATATTATTCTTGTT
ACCGAGGAATTAACTCCATCTGATACCATGCAATTTGACTTAAATTATGTTAAAGGGTTTTTAACTGCAGTTGGAGGAGA
AACTTCTCATGCTGCTATTTTGGCAAGAACAATGGGGCTTCCAGCGCTTGTTATGACTTTGTTAGATATTGATGCGTTAA
AGGATGGTGATAAAATAGTCATTGATGCAATATCTTCTATTGTTATTAAAAATCCTTCTTCTGATGAGCTTGATCTTTAT
AAGGGTAAGATTTTGCGCCAAGTAGAGATGGAAAAAGAGCTTTTTTCTTTAAAAGATAAAGATGCTGAAACAAAAGATGG
TGTAAAAGTATTTTTAAAGGCAAATATTGGAACACCTGTTGATATTGCCTATGTTAATAAATATGGCGTTGAGGGAATAG
GTCTTTTTAGAACAGAATTCTTATATATGAAATCTTTACAACCTCCAACAGAAGATGAACAGTTTGAAACTTATAAGAGA
GTTTTAGATACAATGGAAAAAAAGGGGATTGTTACGATTCGTACTCTTGATGTTGGTGGGGATAAGGAAATTCCCTATCT
TAATTTTGAGAAAGAGGAGAATCCCTTTTTAGGCTTTAGAGCACTTAGGATGTATAAGAAATATGAGGAATTGATCCAAG
CACAGTTTAATGCTATTTTCAGGGCCAGTCATTATGGGAAAATAAGGGTAATGGTGCCTATGCTTACTAGATATGAAGAG
ATTGAAACGATCGAATATTTTGTGAATAATGCAAAAATTAGCTTAAAGTCTAGAGGCTTGCCTTTTGATGAAAACTTAGA
AGTGGGTTGTATGATAGAAGTTCCTTCTGCAGCTTTAATTTCCTCTAAACTTGCCAATAAATTGAAATTTTTTAGCATAG
GGACTAATGATTTAACCCAATATGTTTTAGCTGTTGATCGTGGCAATCAAAAAATATCAAATTTATATGACAAGTATAAT
CCTGCTGTGTTGAAATTAATCAAAAAGGTTCTTGACGATGGAATTAGCTCTGGAATTGATGTGTCTGTTTGTGGCGAGCT
TGGGGGAGATGATGCTGGAGCTTTACTTCTTGTAGGTCTTGGATTTAGGTCTTTAAGCATGATTCCTAGCGCTACACTTA
GAATTAAATATTTGCTTAGAAAGTATACAATAATGGAATTAGAAGAATTGGCAAATAAGGTTTTAAATAGTAATTCTGAG
CAAGAGACTTTAAGTTATTTTGATAAATTTATAGGAGATTAG

Upstream 100 bases:

>100_bases
TTTTGATATGTGCTGAGGGTGAGGATGAGGAGATTGCTGCTTCAGAGCTTGCGGAGCTTATCGAATCTTTTAAAGAATGA
ATTTTAGAAGGATTTAAGTT

Downstream 100 bases:

>100_bases
TTATGGGGTTTTTAGATTTTTTTAAAAAAACCGCTACATTGGATTTGATTGCGCCGATTAGTGGAAAAGTTATGTCAATT
GATAAGGTTCCCGATGAAGC

Product: phosphoenolpyruvate-protein phosphatase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTLSGKRISKGIGIGEVLCIRKNFDKIVSREKIEFSQVDSEISKFNKAKLKAIEALKDLDRKAVLQFGDDKKGIFEGQVL
IVEDDELSELVIELITKENYGAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVTDFSEINKDIILV
TEELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLLDIDALKDGDKIVIDAISSIVIKNPSSDELDLY
KGKILRQVEMEKELFSLKDKDAETKDGVKVFLKANIGTPVDIAYVNKYGVEGIGLFRTEFLYMKSLQPPTEDEQFETYKR
VLDTMEKKGIVTIRTLDVGGDKEIPYLNFEKEENPFLGFRALRMYKKYEELIQAQFNAIFRASHYGKIRVMVPMLTRYEE
IETIEYFVNNAKISLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
PAVLKLIKKVLDDGISSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLRKYTIMELEELANKVLNSNSE
QETLSYFDKFIGD

Sequences:

>Translated_573_residues
MTLSGKRISKGIGIGEVLCIRKNFDKIVSREKIEFSQVDSEISKFNKAKLKAIEALKDLDRKAVLQFGDDKKGIFEGQVL
IVEDDELSELVIELITKENYGAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVTDFSEINKDIILV
TEELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLLDIDALKDGDKIVIDAISSIVIKNPSSDELDLY
KGKILRQVEMEKELFSLKDKDAETKDGVKVFLKANIGTPVDIAYVNKYGVEGIGLFRTEFLYMKSLQPPTEDEQFETYKR
VLDTMEKKGIVTIRTLDVGGDKEIPYLNFEKEENPFLGFRALRMYKKYEELIQAQFNAIFRASHYGKIRVMVPMLTRYEE
IETIEYFVNNAKISLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
PAVLKLIKKVLDDGISSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLRKYTIMELEELANKVLNSNSE
QETLSYFDKFIGD
>Mature_572_residues
TLSGKRISKGIGIGEVLCIRKNFDKIVSREKIEFSQVDSEISKFNKAKLKAIEALKDLDRKAVLQFGDDKKGIFEGQVLI
VEDDELSELVIELITKENYGAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVTDFSEINKDIILVT
EELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLLDIDALKDGDKIVIDAISSIVIKNPSSDELDLYK
GKILRQVEMEKELFSLKDKDAETKDGVKVFLKANIGTPVDIAYVNKYGVEGIGLFRTEFLYMKSLQPPTEDEQFETYKRV
LDTMEKKGIVTIRTLDVGGDKEIPYLNFEKEENPFLGFRALRMYKKYEELIQAQFNAIFRASHYGKIRVMVPMLTRYEEI
ETIEYFVNNAKISLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYNP
AVLKLIKKVLDDGISSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLRKYTIMELEELANKVLNSNSEQ
ETLSYFDKFIGD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=42.031523642732, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1788726, Length=570, Percent_Identity=33.5087719298246, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=33.9920948616601, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI1789193, Length=484, Percent_Identity=31.404958677686, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1787994, Length=440, Percent_Identity=26.1363636363636, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI226510935, Length=145, Percent_Identity=29.6551724137931, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64530; Mature: 64399

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSGKRISKGIGIGEVLCIRKNFDKIVSREKIEFSQVDSEISKFNKAKLKAIEALKDLD
CCCCCCHHHCCCCCCCEEEEHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RKAVLQFGDDKKGIFEGQVLIVEDDELSELVIELITKENYGAAYSIYLAFENLVKSVEDY
HHHHHHCCCCCCCEECCEEEEEECCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
KDPYLKERASDYKDIRNRLISIILGQVTDFSEINKDIILVTEELTPSDTMQFDLNYVKGF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCEEEHHHHHHH
LTAVGGETSHAAILARTMGLPALVMTLLDIDALKDGDKIVIDAISSIVIKNPSSDELDLY
HHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHEECCCCCCCHHHH
KGKILRQVEMEKELFSLKDKDAETKDGVKVFLKANIGTPVDIAYVNKYGVEGIGLFRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHH
LYMKSLQPPTEDEQFETYKRVLDTMEKKGIVTIRTLDVGGDKEIPYLNFEKEENPFLGFR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHH
ALRMYKKYEELIQAQFNAIFRASHYGKIRVMVPMLTRYEEIETIEYFVNNAKISLKSRGL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCC
PFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
CCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCC
PAVLKLIKKVLDDGISSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLR
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHH
KYTIMELEELANKVLNSNSEQETLSYFDKFIGD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLSGKRISKGIGIGEVLCIRKNFDKIVSREKIEFSQVDSEISKFNKAKLKAIEALKDLD
CCCCCHHHCCCCCCCEEEEHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RKAVLQFGDDKKGIFEGQVLIVEDDELSELVIELITKENYGAAYSIYLAFENLVKSVEDY
HHHHHHCCCCCCCEECCEEEEEECCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
KDPYLKERASDYKDIRNRLISIILGQVTDFSEINKDIILVTEELTPSDTMQFDLNYVKGF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCEEEHHHHHHH
LTAVGGETSHAAILARTMGLPALVMTLLDIDALKDGDKIVIDAISSIVIKNPSSDELDLY
HHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHEECCCCCCCHHHH
KGKILRQVEMEKELFSLKDKDAETKDGVKVFLKANIGTPVDIAYVNKYGVEGIGLFRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHH
LYMKSLQPPTEDEQFETYKRVLDTMEKKGIVTIRTLDVGGDKEIPYLNFEKEENPFLGFR
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHH
ALRMYKKYEELIQAQFNAIFRASHYGKIRVMVPMLTRYEEIETIEYFVNNAKISLKSRGL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCC
PFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYN
CCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCC
PAVLKLIKKVLDDGISSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLR
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHCCCHHHHHHHHHH
KYTIMELEELANKVLNSNSEQETLSYFDKFIGD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]