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The map label for this gene is crr [H]

Identifier: 51598810

GI number: 51598810

Start: 573259

End: 573828

Strand: Direct

Name: crr [H]

Synonym: BG0569

Alternate gene names: 51598810

Gene position: 573259-573828 (Clockwise)

Preceding gene: 51598809

Following gene: 51598812

Centisome position: 63.4

GC content: 34.56

Gene sequence:

>570_bases
ATGGGGTTTTTAGATTTTTTTAAAAAAACCGCTACATTGGATTTGATTGCGCCGATTAGTGGAAAAGTTATGTCAATTGA
TAAGGTTCCCGATGAAGCGTTTGCTGAAAAGATAGTTGGTGATGGAATTGCAATTCTTCCAACAAGTAATGAGTTGCTAG
CGCCTTGTGATGGGAAAATAGGCAAAATTTTTAAAACTAATCATGCCTTTAGCCTTGAAACCAAAGAGGGCGTTGAAATT
TTTGTTCATTTTGGAATTAATACTCTTAATTTGAATGGCAGGGGTTTTACAAGAGTTGCCGAGGAGGGCATTAATGTTAA
ACAAGGCGAAGTTATTATTAGGCTTGATCTTGAATATTTAAAAGAGCATTCAGAATCCGTTATTACTCCAGTTGTTATTG
CAAATTCTGATGAAGTTTCAAGCATAGAATATTCTTTTGGAAGACTTGAAAATGAGTCCGAATATATTTTGTCGTCGTCA
ACTGTTTTAACAGAAGAAATTAGACATAAAATATCTCAAACAAAGCCTGTTACAGCAGGCAAAGATTTAGTATTGCGAGT
TAGAAAGTGA

Upstream 100 bases:

>100_bases
ATACAATAATGGAATTAGAAGAATTGGCAAATAAGGTTTTAAATAGTAATTCTGAGCAAGAGACTTTAAGTTATTTTGAT
AAATTTATAGGAGATTAGTT

Downstream 100 bases:

>100_bases
AAAAGAGAGAGTTTTAAACCCTCTCTTTTATTTTTAATCATAACAACTCTTTTTCTAGGAATTCGTTTATTATGTTTATG
AATTTTCCTGGATTTTTGCT

Product: PTS system glucose-specific transporter subunit

Products: Protein Histidine; Sugar Phosphate.; glucose-6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MGFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKIGKIFKTNHAFSLETKEGVEI
FVHFGINTLNLNGRGFTRVAEEGINVKQGEVIIRLDLEYLKEHSESVITPVVIANSDEVSSIEYSFGRLENESEYILSSS
TVLTEEIRHKISQTKPVTAGKDLVLRVRK

Sequences:

>Translated_189_residues
MGFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKIGKIFKTNHAFSLETKEGVEI
FVHFGINTLNLNGRGFTRVAEEGINVKQGEVIIRLDLEYLKEHSESVITPVVIANSDEVSSIEYSFGRLENESEYILSSS
TVLTEEIRHKISQTKPVTAGKDLVLRVRK
>Mature_188_residues
GFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKIGKIFKTNHAFSLETKEGVEIF
VHFGINTLNLNGRGFTRVAEEGINVKQGEVIIRLDLEYLKEHSESVITPVVIANSDEVSSIEYSFGRLENESEYILSSST
VLTEEIRHKISQTKPVTAGKDLVLRVRK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=135, Percent_Identity=58.5185185185185, Blast_Score=170, Evalue=6e-44,
Organism=Escherichia coli, GI1786894, Length=129, Percent_Identity=51.1627906976744, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790159, Length=128, Percent_Identity=35.9375, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 20839; Mature: 20708

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKI
CCHHHHHHHHCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCEEEEECCCCEEECCCCCC
GKIFKTNHAFSLETKEGVEIFVHFGINTLNLNGRGFTRVAEEGINVKQGEVIIRLDLEYL
CCEEECCCEEEEECCCCEEEEEEECCEEEEECCCCHHHHHHCCCCEECCCEEEEEEHHHH
KEHSESVITPVVIANSDEVSSIEYSFGRLENESEYILSSSTVLTEEIRHKISQTKPVTAG
HCCCCCCEEEEEEECCCCCCEEEEHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCC
KDLVLRVRK
CEEEEEECC
>Mature Secondary Structure 
GFLDFFKKTATLDLIAPISGKVMSIDKVPDEAFAEKIVGDGIAILPTSNELLAPCDGKI
CHHHHHHHHCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCEEEEECCCCEEECCCCCC
GKIFKTNHAFSLETKEGVEIFVHFGINTLNLNGRGFTRVAEEGINVKQGEVIIRLDLEYL
CCEEECCCEEEEECCCCEEEEEEECCEEEEECCCCHHHHHHCCCCEECCCEEEEEEHHHH
KEHSESVITPVVIANSDEVSSIEYSFGRLENESEYILSSSTVLTEEIRHKISQTKPVTAG
HCCCCCCEEEEEEECCCCCCEEEEHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCC
KDLVLRVRK
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; beta-D-glucose [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytop

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]