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The map label for this gene is ligA [H]

Identifier: 51598803

GI number: 51598803

Start: 564160

End: 566142

Strand: Direct

Name: ligA [H]

Synonym: BG0562

Alternate gene names: 51598803

Gene position: 564160-566142 (Clockwise)

Preceding gene: 51598796

Following gene: 51598805

Centisome position: 62.39

GC content: 28.95

Gene sequence:

>1983_bases
ATGAGCATTAAAATACAGCAAGAAATTGAAGACTTAAAGAAGCTGATCAGAAAGTGGGATAAAGAGTACTATGTCGATTC
TTTGCCTAGTGTTGAAGATTTTGTATATGATAATCATATTTTAAGGCTTAAAGAGTTGGAAAGTAGGCACCCCGAATATA
AGACCTTGGATTCCCCTACTCTTAAATTTGGTAGCGATCTTTTAAATAACTTTGAAGAGGTTGAACATTCTGTTCCTATA
TTAAGTCTTGATAAGGTTTATGATCTTGATTTACTAAAATCATGGATAGATAAGATTGATTTTAATAATTCTTTTAATAT
TTCTGTTGAACCAAAGATTGATGGATGTTCTATTGTTCTTTATTATAAGGATGGTATTCTTGAAAAAGCTCTTACTAGGG
GTAATGGGAAATTTGGCAATGATGTTACTAAAAACATTAGAACCATTAGGCATATTCCCCTATTTCTTGATGAAAAGGTT
GATTTAGTATTAAGGGGTGAAGTTTATATTACTAAAGAAAATTTTTTTAAAATAAATAAATTTTTGGAAAAGCCTTATAC
TAGTTCTAGAAATTTAGCCTCGGGAATACTTAGAAGGGTTGATAGCAGAGAGGTTGCTAATTTCCCTTTAAATATTTTCA
TTTATGATTTTTTGAATGCTGAACTAGAGCTTAAAACCAATGATTTGGCTATTGCAAAACTTAAAAAATTGGGCTTTAAG
GTCGATCCTTTGATTAGGTTTTTTGATCAAAAAAGTTCAATTATAGAAGTTTTAAATTACATAGCAGATATAACAAAAAA
AAGAAATTCTTTTGAGTATGAAATAGATGGAGTTGTTCTTAAGGTTAGCGAGTTTGTTTTAAGAGAAAAATTAGGGTATA
CCTCGCACCATCCTAAATGGGCAATGGCTTATAAATTTGAAGCGCTTTCGGGTTTTAGTAGGGTAAATAGCATTGTTGTT
CAGGTTGGGCGTAGTGGTAAAATTACTCCGGTTGCTAATATTGATAAAGTTTTTGTTTCAGGGGCTTTTATTACTAACGC
AACATTGCATAATCAAGATTATATAATATCTATTGGTTTAAATGTTGGGGATGTTGTTAAAGTTTCAAGAAGGGGAGATG
TAATTCCTGCTGTTGAAATGGTGATAAATAAATGTTCAAAAGGAGTTTTTAAGGTTCCTGACAATTGTCCAGCTTGTAAA
ACGGTTTTAGTAAAAGAAGGGGCACATTTTTTTTGTACAAATAATAGTTGTCCTTCAGTAGCAGTTGAGAGAATAAAATA
TTTTTGTAGTAAAAATTGTATGGATATTGAAGGGTTTTCCGATAAGACTATTTCTTTTCTTTTTGAGAAAGAATTTATTT
CTTCAGAAATTGATCTTTATACATTTAATTTTTATAAACTTCTTAAATTTAAAGGCTTTAAAGATAGGAAGGTATATAAT
TTGATAAATTCAATTGAAGCTAGCAAAAAAAAACCATTTAGCAAATTACTTCTTAGCATAGGAATTAAAGAGTTGGGAGA
AAACGCCATAAGGTTATTATTTCTTAATAATTTAAATTCATTTTCAAAGCTTTTTAGGCTTTGTCAAGACAGGAATTTTG
CTTTTTCAACATTGTTGAAAATTAAAGGAATAGGGGAGAAAATTGCTTTAAATATTATTGAAGCTTTTAATGATTCAACA
ATGCTTAATAAGTTTAAATTTTTTGAAAATTTGGGATTTAAAATGGAAGAGTCTATTTTGATTGATGATGAAAACAGGTT
ATTAGCTGGTAAAAAGTTTTGTATTACTGGAACTTTTAACGGTTATTCTAGGTCTATTATTATTGACAAACTAAAAAACA
AAGGAGCAATTTTTAAAACTTGTGTTACTGAGGGTTTAGATTTTCTTATTGTAGGAGAAAAAGCTGGATCAAAGCTTGAA
AAAGCTTTAAATTTGAATGTAAAAATTATGTCTTTTGAAGACATAAAGAGTTATTTAAATTGA

Upstream 100 bases:

>100_bases
TTGTGGTACTCTTTTACAGTTTTTATAATAAAAAGGTGGTTTTTGCTTGTGAGTTTAGATTATTTGATAAAATAATAAAG
AATATGTTAAATTTTACCCT

Downstream 100 bases:

>100_bases
TTTAGATTTAAAAAGATTTGATTTTTATTTAATTATTTTTATTTGCTTAAATTTAGGCTTTAGGCTTTTATACATAATTC
CTAGTTCATGGATATTTTTA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSIKIQQEIEDLKKLIRKWDKEYYVDSLPSVEDFVYDNHILRLKELESRHPEYKTLDSPTLKFGSDLLNNFEEVEHSVPI
LSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVLYYKDGILEKALTRGNGKFGNDVTKNIRTIRHIPLFLDEKV
DLVLRGEVYITKENFFKINKFLEKPYTSSRNLASGILRRVDSREVANFPLNIFIYDFLNAELELKTNDLAIAKLKKLGFK
VDPLIRFFDQKSSIIEVLNYIADITKKRNSFEYEIDGVVLKVSEFVLREKLGYTSHHPKWAMAYKFEALSGFSRVNSIVV
QVGRSGKITPVANIDKVFVSGAFITNATLHNQDYIISIGLNVGDVVKVSRRGDVIPAVEMVINKCSKGVFKVPDNCPACK
TVLVKEGAHFFCTNNSCPSVAVERIKYFCSKNCMDIEGFSDKTISFLFEKEFISSEIDLYTFNFYKLLKFKGFKDRKVYN
LINSIEASKKKPFSKLLLSIGIKELGENAIRLLFLNNLNSFSKLFRLCQDRNFAFSTLLKIKGIGEKIALNIIEAFNDST
MLNKFKFFENLGFKMEESILIDDENRLLAGKKFCITGTFNGYSRSIIIDKLKNKGAIFKTCVTEGLDFLIVGEKAGSKLE
KALNLNVKIMSFEDIKSYLN

Sequences:

>Translated_660_residues
MSIKIQQEIEDLKKLIRKWDKEYYVDSLPSVEDFVYDNHILRLKELESRHPEYKTLDSPTLKFGSDLLNNFEEVEHSVPI
LSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVLYYKDGILEKALTRGNGKFGNDVTKNIRTIRHIPLFLDEKV
DLVLRGEVYITKENFFKINKFLEKPYTSSRNLASGILRRVDSREVANFPLNIFIYDFLNAELELKTNDLAIAKLKKLGFK
VDPLIRFFDQKSSIIEVLNYIADITKKRNSFEYEIDGVVLKVSEFVLREKLGYTSHHPKWAMAYKFEALSGFSRVNSIVV
QVGRSGKITPVANIDKVFVSGAFITNATLHNQDYIISIGLNVGDVVKVSRRGDVIPAVEMVINKCSKGVFKVPDNCPACK
TVLVKEGAHFFCTNNSCPSVAVERIKYFCSKNCMDIEGFSDKTISFLFEKEFISSEIDLYTFNFYKLLKFKGFKDRKVYN
LINSIEASKKKPFSKLLLSIGIKELGENAIRLLFLNNLNSFSKLFRLCQDRNFAFSTLLKIKGIGEKIALNIIEAFNDST
MLNKFKFFENLGFKMEESILIDDENRLLAGKKFCITGTFNGYSRSIIIDKLKNKGAIFKTCVTEGLDFLIVGEKAGSKLE
KALNLNVKIMSFEDIKSYLN
>Mature_659_residues
SIKIQQEIEDLKKLIRKWDKEYYVDSLPSVEDFVYDNHILRLKELESRHPEYKTLDSPTLKFGSDLLNNFEEVEHSVPIL
SLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVLYYKDGILEKALTRGNGKFGNDVTKNIRTIRHIPLFLDEKVD
LVLRGEVYITKENFFKINKFLEKPYTSSRNLASGILRRVDSREVANFPLNIFIYDFLNAELELKTNDLAIAKLKKLGFKV
DPLIRFFDQKSSIIEVLNYIADITKKRNSFEYEIDGVVLKVSEFVLREKLGYTSHHPKWAMAYKFEALSGFSRVNSIVVQ
VGRSGKITPVANIDKVFVSGAFITNATLHNQDYIISIGLNVGDVVKVSRRGDVIPAVEMVINKCSKGVFKVPDNCPACKT
VLVKEGAHFFCTNNSCPSVAVERIKYFCSKNCMDIEGFSDKTISFLFEKEFISSEIDLYTFNFYKLLKFKGFKDRKVYNL
INSIEASKKKPFSKLLLSIGIKELGENAIRLLFLNNLNSFSKLFRLCQDRNFAFSTLLKIKGIGEKIALNIIEAFNDSTM
LNKFKFFENLGFKMEESILIDDENRLLAGKKFCITGTFNGYSRSIIIDKLKNKGAIFKTCVTEGLDFLIVGEKAGSKLEK
ALNLNVKIMSFEDIKSYLN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=33.6322869955157, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI87082305, Length=564, Percent_Identity=21.8085106382979, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75526; Mature: 75394

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50172 BRCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKIQQEIEDLKKLIRKWDKEYYVDSLPSVEDFVYDNHILRLKELESRHPEYKTLDSPT
CCEEHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHCCCCEEEHHHHHHCCCCCCCCCCCC
LKFGSDLLNNFEEVEHSVPILSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVL
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
YYKDGILEKALTRGNGKFGNDVTKNIRTIRHIPLFLDEKVDLVLRGEVYITKENFFKINK
EECCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEECEEEEEHHHHHHHHH
FLEKPYTSSRNLASGILRRVDSREVANFPLNIFIYDFLNAELELKTNDLAIAKLKKLGFK
HHHCCCCCCCHHHHHHHHHCCCCHHHCCCEEEEEEEECCCEEEEECCCHHHHHHHHCCCC
VDPLIRFFDQKSSIIEVLNYIADITKKRNSFEYEIDGVVLKVSEFVLREKLGYTSHHPKW
CCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHHCCCCCCCCCE
AMAYKFEALSGFSRVNSIVVQVGRSGKITPVANIDKVFVSGAFITNATLHNQDYIISIGL
EEEEEEHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHEECEEEEEEEECCCCEEEEECC
NVGDVVKVSRRGDVIPAVEMVINKCSKGVFKVPDNCPACKTVLVKEGAHFFCTNNSCPSV
CCCCEEEECCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHEECCCEEEEECCCCHHH
AVERIKYFCSKNCMDIEGFSDKTISFLFEKEFISSEIDLYTFNFYKLLKFKGFKDRKVYN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCHHHHH
LINSIEASKKKPFSKLLLSIGIKELGENAIRLLFLNNLNSFSKLFRLCQDRNFAFSTLLK
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHH
IKGIGEKIALNIIEAFNDSTMLNKFKFFENLGFKMEESILIDDENRLLAGKKFCITGTFN
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCEEEECCCCEEECCEEEEEECCC
GYSRSIIIDKLKNKGAIFKTCVTEGLDFLIVGEKAGSKLEKALNLNVKIMSFEDIKSYLN
CCCCEEEEEHHCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHCCCEEEEEHHHHHHHCC
>Mature Secondary Structure 
SIKIQQEIEDLKKLIRKWDKEYYVDSLPSVEDFVYDNHILRLKELESRHPEYKTLDSPT
CEEHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHCCCCEEEHHHHHHCCCCCCCCCCCC
LKFGSDLLNNFEEVEHSVPILSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVL
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
YYKDGILEKALTRGNGKFGNDVTKNIRTIRHIPLFLDEKVDLVLRGEVYITKENFFKINK
EECCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEECEEEEEHHHHHHHHH
FLEKPYTSSRNLASGILRRVDSREVANFPLNIFIYDFLNAELELKTNDLAIAKLKKLGFK
HHHCCCCCCCHHHHHHHHHCCCCHHHCCCEEEEEEEECCCEEEEECCCHHHHHHHHCCCC
VDPLIRFFDQKSSIIEVLNYIADITKKRNSFEYEIDGVVLKVSEFVLREKLGYTSHHPKW
CCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHHCCCCCCCCCE
AMAYKFEALSGFSRVNSIVVQVGRSGKITPVANIDKVFVSGAFITNATLHNQDYIISIGL
EEEEEEHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHEECEEEEEEEECCCCEEEEECC
NVGDVVKVSRRGDVIPAVEMVINKCSKGVFKVPDNCPACKTVLVKEGAHFFCTNNSCPSV
CCCCEEEECCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHEECCCEEEEECCCCHHH
AVERIKYFCSKNCMDIEGFSDKTISFLFEKEFISSEIDLYTFNFYKLLKFKGFKDRKVYN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCHHHHH
LINSIEASKKKPFSKLLLSIGIKELGENAIRLLFLNNLNSFSKLFRLCQDRNFAFSTLLK
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHH
IKGIGEKIALNIIEAFNDSTMLNKFKFFENLGFKMEESILIDDENRLLAGKKFCITGTFN
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCEEEECCCCEEECCEEEEEECCC
GYSRSIIIDKLKNKGAIFKTCVTEGLDFLIVGEKAGSKLEKALNLNVKIMSFEDIKSYLN
CCCCEEEEEHHCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHCCCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA