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The map label for this gene is trmE

Identifier: 51598233

GI number: 51598233

Start: 4713888

End: 4715252

Strand: Direct

Name: trmE

Synonym: YPTB3949

Alternate gene names: 51598233

Gene position: 4713888-4715252 (Clockwise)

Preceding gene: 51598232

Following gene: 51598234

Centisome position: 99.35

GC content: 51.14

Gene sequence:

>1365_bases
ATGAGCACCACTGATACTATCGTGGCCCAAGCAACGCCCCCTGGTCGTGGCGGTGTCGGCATTCTACGTGTTTCAGGTCG
TGCTGCCTCAGAGGTTGCACACGCCGTTCTGGGTAAATTACCTAAGCCACGTTATGCCGATTATCTGCCGTTTAAAGACG
TGGATGGCAGCACGCTGGATCAAGGTATCGCTCTCTATTTCCCCGGTCCAAATTCTTTCACTGGCGAAGATGTATTAGAG
CTGCAAGGCCATGGTGGGCCGGTGATACTGGATTTACTGTTAAAACGCATTCTAGCCCTGCCAGGGCTGCGTATTGCCCG
CCCTGGGGAGTTTTCCGAGCGTGCCTTCCTCAACGACAAGCTGGACTTAGCACAGGCTGAAGCCATTGCCGACCTGATCG
ACGCCAGTTCAGAGCAAGCAGCACGTTCGGCAGTAAACTCATTGCAAGGCGCATTTTCAGCGCGTATCCATCAATTGGTG
GAAGCACTTACTCACCTGAGAATCTACGTTGAAGCTGCCATTGACTTTCCAGATGAGGAAATTGACTTCCTTTCTGATGG
CAAAATCGAAGGCCAACTGAATGGTGTCATGGCCGATCTGGAACAGGTACGTACTGAGGCTAGACAAGGCAGCTTACTAC
GTGAAGGGATGAAAGTGGTGATTGCTGGCCGGCCAAACGCAGGGAAATCCAGTTTACTCAATGCGTTAGCTGGCCGTGAA
GCGGCGATTGTAACGGATATCGCGGGCACCACCCGTGATGTTTTACGGGAACACATTCATATTGATGGGATGCCTTTACA
TATCATTGATACCGCAGGGTTGCGGGAAGCCAATGACGAAGTTGAGCGCATTGGCATTGAACGCGCGTGGAACGAGATAG
AACAGGCTGATCGTGTATTGTTTATGGTGGATGGCACCACCACCGATGCCACCGAACCTGCGGCTATCTGGCCAGAGTTT
ATGGCACGCTTACCGGCAACACTGCCCATCACTGTGGTACGCAATAAAGCGGATATAACAGGTGAAACGCTGGGGCTGAC
CAAAGTGAATGGTCACTCACTTATTCGTCTGTCGGCACGCACTGGAGAAGGTATTGACCTGCTGCGTGACCATTTAAAAC
AAAGTATGGGCTTTACCAGCAACACTGAAGGCGGTTTCCTGGCTCGACGTCGCCATTTACAGGCACTGGAGACTGCCGCC
AGGCATTTGGTACAAGGCCATGAGCAGTTAGTGAGCGCTTACGCTGGTGAATTACTGGCAGAAGAACTGCGTTTAGCACA
ACAATCATTGAGTGAAATTACCGGTGAATTTAGTTCTGATGACTTACTGGGGCGGATTTTTTCCAGTTTCTGTATCGGAA
AATGA

Upstream 100 bases:

>100_bases
CGCCTTATTCTTTTTACAGAAGCCTGATTTTTTACCTTCAGATTTGTGTAAAAATAATACAGACTTGTTACAAAAATAAT
CGATAAAGAGAGAAATCACC

Downstream 100 bases:

>100_bases
GTGTAATACCATAAAGCTAGCACCAGTTATCGCTAATTTTGATAACCTTACTCTATGGGTTTGGCAGTGGCTCGAACACT
TAACGTTGTATACCCTAAAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSTTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLDQGIALYFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVLFMVDGTTTDATEPAAIWPEF
MARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNTEGGFLARRRHLQALETAA
RHLVQGHEQLVSAYAGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSTTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLDQGIALYFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVLFMVDGTTTDATEPAAIWPEF
MARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNTEGGFLARRRHLQALETAA
RHLVQGHEQLVSAYAGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
>Mature_453_residues
STTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLDQGIALYFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVLFMVDGTTTDATEPAAIWPEFM
ARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSARTGEGIDLLRDHLKQSMGFTSNTEGGFLARRRHLQALETAAR
HLVQGHEQLVSAYAGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=35.7758620689655, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=34.4064386317907, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=34.640522875817, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=88.9867841409692, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=39.5604395604396, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=458, Percent_Identity=28.82096069869, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=31.4401622718053, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI45550813, Length=490, Percent_Identity=32.0408163265306, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49023; Mature: 48892

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLD
CCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QGIALYFPGPNSFTGEDVLELQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDATEPAAIWPEFMARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSAR
EEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCEEEEEECC
TGEGIDLLRDHLKQSMGFTSNTEGGFLARRRHLQALETAARHLVQGHEQLVSAYAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTDTIVAQATPPGRGGVGILRVSGRAASEVAHAVLGKLPKPRYADYLPFKDVDGSTLD
CCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QGIALYFPGPNSFTGEDVLELQGHGGPVILDLLLKRILALPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSAVNSLQGAFSARIHQLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
IDFLSDGKIEGQLNGVMADLEQVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREANDEVERIGIERAWNEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDATEPAAIWPEFMARLPATLPITVVRNKADITGETLGLTKVNGHSLIRLSAR
EEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCEEEEEECC
TGEGIDLLRDHLKQSMGFTSNTEGGFLARRRHLQALETAARHLVQGHEQLVSAYAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA