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The map label for this gene is narK [H]

Identifier: 50841995

GI number: 50841995

Start: 562714

End: 564030

Strand: Reverse

Name: narK [H]

Synonym: PPA0511

Alternate gene names: 50841995

Gene position: 564030-562714 (Counterclockwise)

Preceding gene: 50841999

Following gene: 50841994

Centisome position: 22.03

GC content: 59.0

Gene sequence:

>1317_bases
GTGAGCACAAGCCCTTCCCACCATCTCATCGAGAACTGGGATCCCGAGGAAAACTGGGATTCCGGTATCGCATGGCGAAC
TTTGACCATTTCGACGTTCTCCATGATCATCGCATTTTGCGTATGGTTCTTGGTGAGTGCGATTGCACCCAAACTCAACG
CCATCGGATTCCACCTGACGAAGTCACAGCTGTACTGGCTCACCGCCATGCCAGGGCTCTCGGGAGGATTCATCCGCCTG
GTCTACATGTTCCTACCGCCGGTGATCGGCACTCGCAGGCTCGTGGGATACAGCTCCCTGCTCTACCTCATCCCTATGCT
GGGCTGGTTTTTCGCCGTCCAGAACACGTCGACCGGGTACGGCACCCTTCTCCTCCTCGCCTTCCTATGCGGCGTTGGGG
GCGGTTCTTTCTCCGGTTACATGCCATCAACGGGCTACTTCTTCCCTAAAAGCAAGCAGGGCACCGCTTTGGGAGTACAG
GCTGGCATCGGCAACCTGGGGATGTCCATCGTTCAGTTGCTCGGCCCATGGCTCATGGGCTTCGGGCTCCTCGGCCTCAC
GTGGATCGCCCCGCAGCACACCGCCGGGAGCCCCTTGTGGGTGCACAATATCGCCATCGTCTTCGTCCCGTGGACCATCC
TGGCCACGATTTTGGCATTTACCATGCTCAAAGACGTTCCGGTAAAGGCGAACTTCCGTCGGCAGATCGACATCTTCACC
AACCCCGACACTTGGATCATGACGCTCATGTACGTCATGACGTTCGGCCTGTTCAGCGGCTTCAGCGCCCAGTTCGGGCT
ACTCATCGAGAATACGTATGGCAACTCGAGCAACTTGGCCCACGGATTTTCTAACTTGCCCGCCGGCGCCTCCTACGCCT
TCCTCGGCCCACTGATCGGCTCGTTGGTGCGTGTCGTCTGGGGGCCGCTCTGCGACCGATTCGGTGGGGCGATCTGGACC
CTTGTCTCAGGAATCGGGATGGCACTGACGTTCGGGTTCTGCGCCTTCTTCCTCAACCCCACAAGTCCAAGCCAGTTCGG
CTACTTCCTGTGGGGAATGCTCACCATGTTCTTCTTCTCCGGTATCGGCAACGCCGGGACGTTCAAGCAGATGCCCATGA
TCATGCCTGCCAGACAAGCCGGTGGAGCCATCGGGTTCACCGCCGCCATCGGCTGTTTCGGCCCATTCGTCGTCGGTGTC
GCCCTGTCATCGATGTCAGCCGTTGCCTGGTTCTGGATCTGCACCGTCTTCTGCATTGTGTGCTCGATCATCTGCTGGAT
CCGTTATGCCCGCCCAGGTGCTCCATGCCCTGGCTGA

Upstream 100 bases:

>100_bases
TCGCCACGCCGGCGTTGGGGCACAGGGCGGGGCGGCCTTCACGCCGATGATTTTTTCTGATAATATCCGTGTTAATTGCA
CGATCCCACGCGGAGACATT

Downstream 100 bases:

>100_bases
CCAGCGACCCCAGTTTCGACACACTCCACCTGGTTCGGTTTTTACAAAGGAGACCCGATGACGGAGTACGACGAAACATC
TGACGCCGAGTCCCCCAGCG

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSTSPSHHLIENWDPEENWDSGIAWRTLTISTFSMIIAFCVWFLVSAIAPKLNAIGFHLTKSQLYWLTAMPGLSGGFIRL
VYMFLPPVIGTRRLVGYSSLLYLIPMLGWFFAVQNTSTGYGTLLLLAFLCGVGGGSFSGYMPSTGYFFPKSKQGTALGVQ
AGIGNLGMSIVQLLGPWLMGFGLLGLTWIAPQHTAGSPLWVHNIAIVFVPWTILATILAFTMLKDVPVKANFRRQIDIFT
NPDTWIMTLMYVMTFGLFSGFSAQFGLLIENTYGNSSNLAHGFSNLPAGASYAFLGPLIGSLVRVVWGPLCDRFGGAIWT
LVSGIGMALTFGFCAFFLNPTSPSQFGYFLWGMLTMFFFSGIGNAGTFKQMPMIMPARQAGGAIGFTAAIGCFGPFVVGV
ALSSMSAVAWFWICTVFCIVCSIICWIRYARPGAPCPG

Sequences:

>Translated_438_residues
MSTSPSHHLIENWDPEENWDSGIAWRTLTISTFSMIIAFCVWFLVSAIAPKLNAIGFHLTKSQLYWLTAMPGLSGGFIRL
VYMFLPPVIGTRRLVGYSSLLYLIPMLGWFFAVQNTSTGYGTLLLLAFLCGVGGGSFSGYMPSTGYFFPKSKQGTALGVQ
AGIGNLGMSIVQLLGPWLMGFGLLGLTWIAPQHTAGSPLWVHNIAIVFVPWTILATILAFTMLKDVPVKANFRRQIDIFT
NPDTWIMTLMYVMTFGLFSGFSAQFGLLIENTYGNSSNLAHGFSNLPAGASYAFLGPLIGSLVRVVWGPLCDRFGGAIWT
LVSGIGMALTFGFCAFFLNPTSPSQFGYFLWGMLTMFFFSGIGNAGTFKQMPMIMPARQAGGAIGFTAAIGCFGPFVVGV
ALSSMSAVAWFWICTVFCIVCSIICWIRYARPGAPCPG
>Mature_437_residues
STSPSHHLIENWDPEENWDSGIAWRTLTISTFSMIIAFCVWFLVSAIAPKLNAIGFHLTKSQLYWLTAMPGLSGGFIRLV
YMFLPPVIGTRRLVGYSSLLYLIPMLGWFFAVQNTSTGYGTLLLLAFLCGVGGGSFSGYMPSTGYFFPKSKQGTALGVQA
GIGNLGMSIVQLLGPWLMGFGLLGLTWIAPQHTAGSPLWVHNIAIVFVPWTILATILAFTMLKDVPVKANFRRQIDIFTN
PDTWIMTLMYVMTFGLFSGFSAQFGLLIENTYGNSSNLAHGFSNLPAGASYAFLGPLIGSLVRVVWGPLCDRFGGAIWTL
VSGIGMALTFGFCAFFLNPTSPSQFGYFLWGMLTMFFFSGIGNAGTFKQMPMIMPARQAGGAIGFTAAIGCFGPFVVGVA
LSSMSAVAWFWICTVFCIVCSIICWIRYARPGAPCPG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=470, Percent_Identity=33.8297872340426, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787743, Length=465, Percent_Identity=33.5483870967742, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47545; Mature: 47413

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSPSHHLIENWDPEENWDSGIAWRTLTISTFSMIIAFCVWFLVSAIAPKLNAIGFHLT
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEE
KSQLYWLTAMPGLSGGFIRLVYMFLPPVIGTRRLVGYSSLLYLIPMLGWFFAVQNTSTGY
CCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
GTLLLLAFLCGVGGGSFSGYMPSTGYFFPKSKQGTALGVQAGIGNLGMSIVQLLGPWLMG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHH
FGLLGLTWIAPQHTAGSPLWVHNIAIVFVPWTILATILAFTMLKDVPVKANFRRQIDIFT
HHHHHHHHCCCCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
NPDTWIMTLMYVMTFGLFSGFSAQFGLLIENTYGNSSNLAHGFSNLPAGASYAFLGPLIG
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SLVRVVWGPLCDRFGGAIWTLVSGIGMALTFGFCAFFLNPTSPSQFGYFLWGMLTMFFFS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
GIGNAGTFKQMPMIMPARQAGGAIGFTAAIGCFGPFVVGVALSSMSAVAWFWICTVFCIV
CCCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSIICWIRYARPGAPCPG
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STSPSHHLIENWDPEENWDSGIAWRTLTISTFSMIIAFCVWFLVSAIAPKLNAIGFHLT
CCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEE
KSQLYWLTAMPGLSGGFIRLVYMFLPPVIGTRRLVGYSSLLYLIPMLGWFFAVQNTSTGY
CCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
GTLLLLAFLCGVGGGSFSGYMPSTGYFFPKSKQGTALGVQAGIGNLGMSIVQLLGPWLMG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHH
FGLLGLTWIAPQHTAGSPLWVHNIAIVFVPWTILATILAFTMLKDVPVKANFRRQIDIFT
HHHHHHHHCCCCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
NPDTWIMTLMYVMTFGLFSGFSAQFGLLIENTYGNSSNLAHGFSNLPAGASYAFLGPLIG
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SLVRVVWGPLCDRFGGAIWTLVSGIGMALTFGFCAFFLNPTSPSQFGYFLWGMLTMFFFS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
GIGNAGTFKQMPMIMPARQAGGAIGFTAAIGCFGPFVVGVALSSMSAVAWFWICTVFCIV
CCCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSIICWIRYARPGAPCPG
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]