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The map label for this gene is moaE2 [H]

Identifier: 50841983

GI number: 50841983

Start: 548936

End: 550270

Strand: Reverse

Name: moaE2 [H]

Synonym: PPA0499

Alternate gene names: 50841983

Gene position: 550270-548936 (Counterclockwise)

Preceding gene: 50841984

Following gene: 50841982

Centisome position: 21.49

GC content: 65.39

Gene sequence:

>1335_bases
GTGAGCAGCTCTCGTTCCAGCGTGCCCGCCACGACGCCAAAGCCAGTGAGCATCGCACGGCACCTGCAACTGGTCTCCTC
ACTGGCTACCGCACCCAAGACAGTCGAACTACCGCTGGCCGAGTGTCTGGGGCTAGTCAGCGCCGAACCAGTTACCGCCC
GACTGACGGTACCGCCGTTCACCAACTCTGCGATGGACGGGTTCGCGGTGCGTCACGCCGACGTGTCCGAGGCCACTGTG
CACACCCCTGTCACGCTCCCGGTCACCAATGACGTACCCGCCGGATCTCCCGCCCACCGGCCTCTCGACCCCGGAACCGC
CCAGCGCATCATGACCGGTGCCCGCATGCCCGAGAACGCCGACACCGTCATCAAGGTGGAGGACACCGATCATGCTCCCG
GAACTGTCGACGCCCCCGCGACGGTGACGATTTTTCGCACCCCCTCAACCGGTGCCAATGTGCGACGCCGTGGTGAGGCT
CTGGCCACCGGTGCCACGGTGCTGCCTGCCGGGAGAAGACTGACCCCCGAGGCACTGGCCGCTGCAGCCGCAGTAGGTCA
TGGCAGTCTGCGAGTTCACCCGGCCCCTCGGGTCGGTGTCCTTGCGACAGGTTCGGAGCTTGGCGGGTCTGGTGAACCGC
TACATCGTGGCAAAATCCCCGATTCCAACGGCATCATGTTGGGTGGTCTGGTGCGCACACACGGAGGAGTCGTCGAGCAA
ACCCGCGTACCCGACGACCCGGAACGCCTGCGCGAACTCGTCCGCTCCTGGCGGGTGGACCTGATTGTCACAGCTGGAGG
CATCTCCATGGGGGCCTACGAGGTCGTGCGGCAGGGGCTGCCCGAACTCACCTTCCACCATGTGGCCCAGCAACCCGGAG
GCCCCCAAGGGGCTGGAATGGTGGCCGAGATGCCCGTGCTCGCCCTACCGGGCAACCCGGTCGGGGCCTACGTGAGTTTC
CACGTATACGTCGCCCCACTGCTGGCACGGTTACGCGGCCTGTCACCCGATCTCATTGACGCGACACCCGCCGGAGTGAC
AGCACCCATAGACCCGCTGCTGGGACGTCGCCTGGTACGTGCCCGAGCCGGTGCTCCGTGGCGCTCCCCAGCAGCCAAGA
CTCAGTTCATGCCAGTGTGTTGGAACGGCGAGAATGTCGAGCCGGTGCACGCGCTGGGGTCAAAATCCCACTTAGTGACA
AGTCTGCCTCTGGTAGAGGGCCTGGCGGTGGCGGGTCCCGGAATCGATCACGTGGACTCCGGCGACGAGTTGAGCATCCT
ACTGACGGGAGGAGTTACACATGACCTCACACCCATTCACCCACCTGCGTGGTAA

Upstream 100 bases:

>100_bases
GTGAATTCGTCGACGCCGTTAAGGAAAGCCTGCCGGTGTGGAAGTGCCAATGGCTCACTGACGGCTCCCACGAATGGGCC
GGGCTGCCCGGGGAGGAGCC

Downstream 100 bases:

>100_bases
CGGATCGGCTCACATGGTCGACGTCACGACAAAGACACCCACGGTGCGCGTGGCGACGGCGGAATGCTGCGTGGTGTGCT
CCGAGCCGATCATGACTGCC

Product: molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: MPT Mo-transferase 2 [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSSSRSSVPATTPKPVSIARHLQLVSSLATAPKTVELPLAECLGLVSAEPVTARLTVPPFTNSAMDGFAVRHADVSEATV
HTPVTLPVTNDVPAGSPAHRPLDPGTAQRIMTGARMPENADTVIKVEDTDHAPGTVDAPATVTIFRTPSTGANVRRRGEA
LATGATVLPAGRRLTPEALAAAAAVGHGSLRVHPAPRVGVLATGSELGGSGEPLHRGKIPDSNGIMLGGLVRTHGGVVEQ
TRVPDDPERLRELVRSWRVDLIVTAGGISMGAYEVVRQGLPELTFHHVAQQPGGPQGAGMVAEMPVLALPGNPVGAYVSF
HVYVAPLLARLRGLSPDLIDATPAGVTAPIDPLLGRRLVRARAGAPWRSPAAKTQFMPVCWNGENVEPVHALGSKSHLVT
SLPLVEGLAVAGPGIDHVDSGDELSILLTGGVTHDLTPIHPPAW

Sequences:

>Translated_444_residues
MSSSRSSVPATTPKPVSIARHLQLVSSLATAPKTVELPLAECLGLVSAEPVTARLTVPPFTNSAMDGFAVRHADVSEATV
HTPVTLPVTNDVPAGSPAHRPLDPGTAQRIMTGARMPENADTVIKVEDTDHAPGTVDAPATVTIFRTPSTGANVRRRGEA
LATGATVLPAGRRLTPEALAAAAAVGHGSLRVHPAPRVGVLATGSELGGSGEPLHRGKIPDSNGIMLGGLVRTHGGVVEQ
TRVPDDPERLRELVRSWRVDLIVTAGGISMGAYEVVRQGLPELTFHHVAQQPGGPQGAGMVAEMPVLALPGNPVGAYVSF
HVYVAPLLARLRGLSPDLIDATPAGVTAPIDPLLGRRLVRARAGAPWRSPAAKTQFMPVCWNGENVEPVHALGSKSHLVT
SLPLVEGLAVAGPGIDHVDSGDELSILLTGGVTHDLTPIHPPAW
>Mature_443_residues
SSSRSSVPATTPKPVSIARHLQLVSSLATAPKTVELPLAECLGLVSAEPVTARLTVPPFTNSAMDGFAVRHADVSEATVH
TPVTLPVTNDVPAGSPAHRPLDPGTAQRIMTGARMPENADTVIKVEDTDHAPGTVDAPATVTIFRTPSTGANVRRRGEAL
ATGATVLPAGRRLTPEALAAAAAVGHGSLRVHPAPRVGVLATGSELGGSGEPLHRGKIPDSNGIMLGGLVRTHGGVVEQT
RVPDDPERLRELVRSWRVDLIVTAGGISMGAYEVVRQGLPELTFHHVAQQPGGPQGAGMVAEMPVLALPGNPVGAYVSFH
VYVAPLLARLRGLSPDLIDATPAGVTAPIDPLLGRRLVRARAGAPWRSPAAKTQFMPVCWNGENVEPVHALGSKSHLVTS
LPLVEGLAVAGPGIDHVDSGDELSILLTGGVTHDLTPIHPPAW

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI66932975, Length=306, Percent_Identity=32.0261437908497, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI10880983, Length=306, Percent_Identity=32.0261437908497, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1787049, Length=310, Percent_Identity=37.4193548387097, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147659, Length=352, Percent_Identity=31.5340909090909, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17562606, Length=333, Percent_Identity=24.024024024024, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136978, Length=288, Percent_Identity=32.6388888888889, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24638841, Length=288, Percent_Identity=32.6388888888889, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 46015; Mature: 45884

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSRSSVPATTPKPVSIARHLQLVSSLATAPKTVELPLAECLGLVSAEPVTARLTVPPF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCEEEEEECCCC
TNSAMDGFAVRHADVSEATVHTPVTLPVTNDVPAGSPAHRPLDPGTAQRIMTGARMPENA
CCCCCCCEEEEECCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
DTVIKVEDTDHAPGTVDAPATVTIFRTPSTGANVRRRGEALATGATVLPAGRRLTPEALA
CCEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHCHHHHCCCEEECCCCCCCHHHHH
AAAAVGHGSLRVHPAPRVGVLATGSELGGSGEPLHRGKIPDSNGIMLGGLVRTHGGVVEQ
HHHHHCCCCEEECCCCCEEEEEECHHCCCCCCCCCCCCCCCCCCEEECCCHHHCCCEEEC
TRVPDDPERLRELVRSWRVDLIVTAGGISMGAYEVVRQGLPELTFHHVAQQPGGPQGAGM
CCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCE
VAEMPVLALPGNPVGAYVSFHVYVAPLLARLRGLSPDLIDATPAGVTAPIDPLLGRRLVR
EECCCEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ARAGAPWRSPAAKTQFMPVCWNGENVEPVHALGSKSHLVTSLPLVEGLAVAGPGIDHVDS
HHCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCC
GDELSILLTGGVTHDLTPIHPPAW
CCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSSRSSVPATTPKPVSIARHLQLVSSLATAPKTVELPLAECLGLVSAEPVTARLTVPPF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCEEEEEECCCC
TNSAMDGFAVRHADVSEATVHTPVTLPVTNDVPAGSPAHRPLDPGTAQRIMTGARMPENA
CCCCCCCEEEEECCCCCCEEECCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
DTVIKVEDTDHAPGTVDAPATVTIFRTPSTGANVRRRGEALATGATVLPAGRRLTPEALA
CCEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHCHHHHCCCEEECCCCCCCHHHHH
AAAAVGHGSLRVHPAPRVGVLATGSELGGSGEPLHRGKIPDSNGIMLGGLVRTHGGVVEQ
HHHHHCCCCEEECCCCCEEEEEECHHCCCCCCCCCCCCCCCCCCEEECCCHHHCCCEEEC
TRVPDDPERLRELVRSWRVDLIVTAGGISMGAYEVVRQGLPELTFHHVAQQPGGPQGAGM
CCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCE
VAEMPVLALPGNPVGAYVSFHVYVAPLLARLRGLSPDLIDATPAGVTAPIDPLLGRRLVR
EECCCEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ARAGAPWRSPAAKTQFMPVCWNGENVEPVHALGSKSHLVTSLPLVEGLAVAGPGIDHVDS
HHCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCC
GDELSILLTGGVTHDLTPIHPPAW
CCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]