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The map label for this gene is ispF

Identifier: 50841840

GI number: 50841840

Start: 405058

End: 405564

Strand: Direct

Name: ispF

Synonym: PPA0354

Alternate gene names: 50841840

Gene position: 405058-405564 (Clockwise)

Preceding gene: 50841839

Following gene: 50841842

Centisome position: 15.82

GC content: 61.14

Gene sequence:

>507_bases
GTGATAGAAATCCGAACGGGGATCGGGACGGATGTCCATCAGTTAAGCGCGGGCGTGCCGATGCATGTTGCCGGGCTTTC
TTTCCCTGATGAGACGGTTGGGCTAGTCGGTCATTCTGACGGTGACGTTGCCTGCCACGCCATATGCGATGCGCTGTTAT
CGGCAACCGGACTGGGTGACATTGGCTCAATATTTGGCACCGCGGATCCGCAGTGGGCTGGGGCGGCTGGTGTCGCCTTA
CTTGGTCATGTGGTAGCTCTCGTCACCGGGGAAGGATGGACAATCCAGAACGTCGCGGTGCAGGTTGTTGGTCAGAGACC
GCGTATGGCCGCTCGCAGGGCCGAGGCGGAGGCCGCCCTGGCACAAACCGTCGGGGCTCCGGTTTCGGTGTCGGCGACGA
CGACCGATCACTTGGGGTTTACCGGACGTGGTGAGGGAGTAGCGGCGATTGCATCAGCCCTTGTCACTCGAGGGCTCAAA
GGTGAGGATAGCTCCCTGATCCGGTAG

Upstream 100 bases:

>100_bases
CTGCCGGTGCCGGGCGTCATTCGGGACGTCGTATTGGCAGGATGCTGCATGCTGTCAAGCCGTCTACCGTTGCTAGGAAA
TTGGGTCGCGGGAGTCGGCG

Downstream 100 bases:

>100_bases
CTGCGGTGGTGGCCCGGACATCTTGATAGCTCCAGCAAGTAACGATTGGTCGGGGGAGTTGAAATACAACACGACGTGGC
CGAGGTCGCCCAGGTTTGAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIEIRTGIGTDVHQLSAGVPMHVAGLSFPDETVGLVGHSDGDVACHAICDALLSATGLGDIGSIFGTADPQWAGAAGVAL
LGHVVALVTGEGWTIQNVAVQVVGQRPRMAARRAEAEAALAQTVGAPVSVSATTTDHLGFTGRGEGVAAIASALVTRGLK
GEDSSLIR

Sequences:

>Translated_168_residues
MIEIRTGIGTDVHQLSAGVPMHVAGLSFPDETVGLVGHSDGDVACHAICDALLSATGLGDIGSIFGTADPQWAGAAGVAL
LGHVVALVTGEGWTIQNVAVQVVGQRPRMAARRAEAEAALAQTVGAPVSVSATTTDHLGFTGRGEGVAAIASALVTRGLK
GEDSSLIR
>Mature_168_residues
MIEIRTGIGTDVHQLSAGVPMHVAGLSFPDETVGLVGHSDGDVACHAICDALLSATGLGDIGSIFGTADPQWAGAAGVAL
LGHVVALVTGEGWTIQNVAVQVVGQRPRMAARRAEAEAALAQTVGAPVSVSATTTDHLGFTGRGEGVAAIASALVTRGLK
GEDSSLIR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=160, Percent_Identity=42.5, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PROAC (Q6AAV7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055067.1
- ProteinModelPortal:   Q6AAV7
- SMR:   Q6AAV7
- GeneID:   2932069
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0354
- NMPDR:   fig|267747.1.peg.344
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   PACN267747:PPA0354-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16829; Mature: 16829

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIRTGIGTDVHQLSAGVPMHVAGLSFPDETVGLVGHSDGDVACHAICDALLSATGLGD
CEEECCCCCCCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
IGSIFGTADPQWAGAAGVALLGHVVALVTGEGWTIQNVAVQVVGQRPRMAARRAEAEAAL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
AQTVGAPVSVSATTTDHLGFTGRGEGVAAIASALVTRGLKGEDSSLIR
HHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIEIRTGIGTDVHQLSAGVPMHVAGLSFPDETVGLVGHSDGDVACHAICDALLSATGLGD
CEEECCCCCCCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
IGSIFGTADPQWAGAAGVALLGHVVALVTGEGWTIQNVAVQVVGQRPRMAARRAEAEAAL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
AQTVGAPVSVSATTTDHLGFTGRGEGVAAIASALVTRGLKGEDSSLIR
HHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA