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The map label for this gene is ispD

Identifier: 50841839

GI number: 50841839

Start: 404249

End: 405061

Strand: Direct

Name: ispD

Synonym: PPA0353

Alternate gene names: 50841839

Gene position: 404249-405061 (Clockwise)

Preceding gene: 50841835

Following gene: 50841840

Centisome position: 15.79

GC content: 58.92

Gene sequence:

>813_bases
GTGCCAACCATGGTTGATGAGCACACCCCGCCGGTCGTCGCGGTTGTCGTCGCAGCCGGTCTGGGGACACGATTCGGCGG
TCCGGTTCCCAAGCAAGTTACCTCCCTGACCGGAAAGGCCGTCGTCGCTGTTGCTGTGGAATCCCTAGCTGCTGGCGGTT
GTGACGAGGCCGTTGTCGTCGTGAAGGAGGGCATGCACAACCACTTACAGCTAGCCCTAGCTGCATCTCCGATACCGGTT
CACTTCGTTACTGGGGGCAATACGCGCCAGGAATCGGTACGCAACGGGCTGAGGTTTATCGCTCAACATCATCGGTTGAG
CGACGCTACAACGGTGCTCATTCACGATGCAGTGCGTCCGCTGGTACCGGCATACGTCGTAGAGAATGTTATCGCGGCCG
TGGAGAATGGAGCTGTTGCCGTGACTCCGGTGGTCGATGTGGTCGACACCATACGTCAGGTTGACGGAGACACGTCGTCG
GTCGTTGACCGTTCGACCCTGCGTGCTGTTCAGACCCCCCAGGGATTTAAACGGGACATCATCACTGAATGTCACGAGCA
GCTATCTATCGAGGGCGGTTCGGTGACTGACGACATTTCGTGTTGTGAGCGATACGGCCACCATGCCACCTTCGTCGAAG
GCTCCAGAATGTCCCTCAAGATTACTGAGCCGGTCGATCTCGACGTCGCAGAGGTGTTTGCGAAAGCGGCTGCCGGTGCC
GGGCGTCATTCGGGACGTCGTATTGGCAGGATGCTGCATGCTGTCAAGCCGTCTACCGTTGCTAGGAAATTGGGTCGCGG
GAGTCGGCGGTGA

Upstream 100 bases:

>100_bases
GTGCGGCTCGGCATGATTGCCTCCTTGGTGGGCTGAAAATGTCAGGGACGACGATAGCCGACTGCGCGATGATACGACGT
GGTGGGTCGGGGCCGATAGA

Downstream 100 bases:

>100_bases
TAGAAATCCGAACGGGGATCGGGACGGATGTCCATCAGTTAAGCGCGGGCGTGCCGATGCATGTTGCCGGGCTTTCTTTC
CCTGATGAGACGGTTGGGCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPTMVDEHTPPVVAVVVAAGLGTRFGGPVPKQVTSLTGKAVVAVAVESLAAGGCDEAVVVVKEGMHNHLQLALAASPIPV
HFVTGGNTRQESVRNGLRFIAQHHRLSDATTVLIHDAVRPLVPAYVVENVIAAVENGAVAVTPVVDVVDTIRQVDGDTSS
VVDRSTLRAVQTPQGFKRDIITECHEQLSIEGGSVTDDISCCERYGHHATFVEGSRMSLKITEPVDLDVAEVFAKAAAGA
GRHSGRRIGRMLHAVKPSTVARKLGRGSRR

Sequences:

>Translated_270_residues
MPTMVDEHTPPVVAVVVAAGLGTRFGGPVPKQVTSLTGKAVVAVAVESLAAGGCDEAVVVVKEGMHNHLQLALAASPIPV
HFVTGGNTRQESVRNGLRFIAQHHRLSDATTVLIHDAVRPLVPAYVVENVIAAVENGAVAVTPVVDVVDTIRQVDGDTSS
VVDRSTLRAVQTPQGFKRDIITECHEQLSIEGGSVTDDISCCERYGHHATFVEGSRMSLKITEPVDLDVAEVFAKAAAGA
GRHSGRRIGRMLHAVKPSTVARKLGRGSRR
>Mature_269_residues
PTMVDEHTPPVVAVVVAAGLGTRFGGPVPKQVTSLTGKAVVAVAVESLAAGGCDEAVVVVKEGMHNHLQLALAASPIPVH
FVTGGNTRQESVRNGLRFIAQHHRLSDATTVLIHDAVRPLVPAYVVENVIAAVENGAVAVTPVVDVVDTIRQVDGDTSSV
VDRSTLRAVQTPQGFKRDIITECHEQLSIEGGSVTDDISCCERYGHHATFVEGSRMSLKITEPVDLDVAEVFAKAAAGAG
RHSGRRIGRMLHAVKPSTVARKLGRGSRR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=241, Percent_Identity=32.7800829875519, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=32.7433628318584, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PROAC (Q6AAV8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055066.1
- ProteinModelPortal:   Q6AAV8
- SMR:   Q6AAV8
- GeneID:   2932096
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0353
- NMPDR:   fig|267747.1.peg.343
- HOGENOM:   HBG672839
- OMA:   PYNEQIF
- ProtClustDB:   CLSK2763239
- BioCyc:   PACN267747:PPA0353-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 28502; Mature: 28371

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTMVDEHTPPVVAVVVAAGLGTRFGGPVPKQVTSLTGKAVVAVAVESLAAGGCDEAVVV
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHE
VKEGMHNHLQLALAASPIPVHFVTGGNTRQESVRNGLRFIAQHHRLSDATTVLIHDAVRP
EHHCCCHHEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LVPAYVVENVIAAVENGAVAVTPVVDVVDTIRQVDGDTSSVVDRSTLRAVQTPQGFKRDI
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
ITECHEQLSIEGGSVTDDISCCERYGHHATFVEGSRMSLKITEPVDLDVAEVFAKAAAGA
HHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHCCC
GRHSGRRIGRMLHAVKPSTVARKLGRGSRR
CCCHHHHHHHHHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
PTMVDEHTPPVVAVVVAAGLGTRFGGPVPKQVTSLTGKAVVAVAVESLAAGGCDEAVVV
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCEEHE
VKEGMHNHLQLALAASPIPVHFVTGGNTRQESVRNGLRFIAQHHRLSDATTVLIHDAVRP
EHHCCCHHEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LVPAYVVENVIAAVENGAVAVTPVVDVVDTIRQVDGDTSSVVDRSTLRAVQTPQGFKRDI
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
ITECHEQLSIEGGSVTDDISCCERYGHHATFVEGSRMSLKITEPVDLDVAEVFAKAAAGA
HHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHCCC
GRHSGRRIGRMLHAVKPSTVARKLGRGSRR
CCCHHHHHHHHHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA