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The map label for this gene is ptsI [H]

Identifier: 50841837

GI number: 50841837

Start: 402218

End: 403936

Strand: Reverse

Name: ptsI [H]

Synonym: PPA0351

Alternate gene names: 50841837

Gene position: 403936-402218 (Counterclockwise)

Preceding gene: 50841838

Following gene: 50841836

Centisome position: 15.78

GC content: 64.05

Gene sequence:

>1719_bases
ATGCCCTCGTTGCGAACCTGCAAACCGACCCGGAGGCCTGATCCCATGCGGACCCTCTCGGGGCTCGGGGTCTCGGCAGG
AGTCGGACAGGCCCACGCGCTTGTCGTCTCCCCCGCCCCCGGACTGCCCGCTTCAGATCCCAGCCGAGGCGCAGAGGCAG
ACCTGGCTAAGGTTGAGGCTGCCCTCGGCAAGGTTGCTGATCGTCTCGATGAGCGTTCCCTTCACGCCGATCCGTCGGCT
CTTGCAGTACTGCAGGCCACTGCCATGATGGCTCGCGATCCCGGGCTGCTTACCAGCATCAAGTCCCACCTATCCGACGG
GCATGGTCCGGCCCACGCGTTGTGGGCAGCCTTTGAGGATTTCTGCGCTCAGCTGAGCGCAGCTGGCGGCTATCTCGCCG
AACGCGTCACCGACTTGCGCAACGTCCGCGATCGCGCGGTCGCCGTCATGCAAGGACTGCCTGAGCCCGGTGTTCCCTCC
TTTGATTCGCCAGTGATTCTCGTCGCGGAAGATCTCGCCCCGGCTGACACTGCGACCCTCAACCCAGAGCTGGTCCGTGG
TCTCATCACTGCGGCTGGCGGCCCGACAAGCCATACCGCTATTCTGGCCTCCCAGATCGGTATTCCCGCTGTGGTGAGGT
GTAGCGAGGCTCGTGACATTGAAGATGGCACTCCGCTAGCCCTTGACGGTGTCACCGGCACGGTCCTTGTCGAGCCCGAC
GAGGCATCCGTCTCCGAGCTCACCGAGCGTGCCAATCGCCGTGCCGAGGTGTTGGCATCAGCCCCTGACGGCGACGCCAC
ACTGTCTGACGGCGAACGCATCCTCGTACTCGCCAACATCGGCAACCCTTCCGACGCCCCCACGGCGAAGAAGAAAGGCG
CCGAGGGAGTCGGCCTGTTCCGCACCGAGTTCCTCTTCTTGGACCGGCAAGACGCACCCACTATTGACGAGCAAACTGAG
GCGTACGCCACCGTACTTAAGACCTTTGATGAGGGCGCCAAGGTTGTCTTTCGTACCCTAGACGTCGGAGCCGACAAGCC
GTTGGCGTTTGCCGACCTGGGCGCTGAGGAAAACCCGGCCCTAGGACGTCGCGGGCTGCGTCTGTCCCAGGTGCGCACCG
ACCTCATCGACGCCCAGCTGGAGGCACTCGCCAAGGCTGCTGAGCAGACAGGTCGTGACCCGTACGTGATGGCCCCGATG
GTCGCCACGAAGGCAGAAGCCGAGTGGTTCTCCTCTCGTGCCCGTGCCGCCGGTATCAAGACCGTAGGCATCATGGTCGA
GGTGCCCTCGACGGCTCTACGGTCGTCCCAGGTACTGGAGTCGGTAGATTTTGCCTCCATTGGCACTAACGACCTCACCC
AGTACGCAATGGCCGCTGACCGCCTGCAAGGCGAGTTGGCCGAACTCTTGACGCCGTGGCAGCCCGCCGTCATTCAGCTG
GTCAAAGCCACTTGTGATGGAGCTGGCGAGCGGCTTATTGGCGTGTGCGGAGAGGCTGCTGGTGATCCTCTGTTGGCCTT
GGTCCTCGTGGGCGTCGGAGTCCGTTCGCTCTCGATGGCCGCCGGCAAGATCACCGCCGTCAAGGCGGCCCTATCTCGTC
ATAACCTAGAGCAGTGCCGCCGAATTGCCGACGCCGCTCTGGCCGCTTGCAGCCCAGAAGAGGCCCGCACGGCCGCCCTG
AAGCTAGCTGACCCGAGTGTCGAGGTTCTTGTCAGCTGA

Upstream 100 bases:

>100_bases
GTGGATGCCCGCTCAATTTTGTCGGTGATGGGGCTTGGCGCCAAGCACGGCGAGACCGTCGAATTGACCGCAGAGGGTGA
TAACGCCGACGAGGTGCTTG

Downstream 100 bases:

>100_bases
TACACGATCCTCAATCCGCTAAAGCCCCCGCAGAGGGGGCTTTAGCATGCTCCGCAGGGGGAGGACAATAGGGCCATGAC
TAAAACTGCAGTGGTCACAG

Product: putative phosphoenolpyruvate-protein kinase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MPSLRTCKPTRRPDPMRTLSGLGVSAGVGQAHALVVSPAPGLPASDPSRGAEADLAKVEAALGKVADRLDERSLHADPSA
LAVLQATAMMARDPGLLTSIKSHLSDGHGPAHALWAAFEDFCAQLSAAGGYLAERVTDLRNVRDRAVAVMQGLPEPGVPS
FDSPVILVAEDLAPADTATLNPELVRGLITAAGGPTSHTAILASQIGIPAVVRCSEARDIEDGTPLALDGVTGTVLVEPD
EASVSELTERANRRAEVLASAPDGDATLSDGERILVLANIGNPSDAPTAKKKGAEGVGLFRTEFLFLDRQDAPTIDEQTE
AYATVLKTFDEGAKVVFRTLDVGADKPLAFADLGAEENPALGRRGLRLSQVRTDLIDAQLEALAKAAEQTGRDPYVMAPM
VATKAEAEWFSSRARAAGIKTVGIMVEVPSTALRSSQVLESVDFASIGTNDLTQYAMAADRLQGELAELLTPWQPAVIQL
VKATCDGAGERLIGVCGEAAGDPLLALVLVGVGVRSLSMAAGKITAVKAALSRHNLEQCRRIADAALAACSPEEARTAAL
KLADPSVEVLVS

Sequences:

>Translated_572_residues
MPSLRTCKPTRRPDPMRTLSGLGVSAGVGQAHALVVSPAPGLPASDPSRGAEADLAKVEAALGKVADRLDERSLHADPSA
LAVLQATAMMARDPGLLTSIKSHLSDGHGPAHALWAAFEDFCAQLSAAGGYLAERVTDLRNVRDRAVAVMQGLPEPGVPS
FDSPVILVAEDLAPADTATLNPELVRGLITAAGGPTSHTAILASQIGIPAVVRCSEARDIEDGTPLALDGVTGTVLVEPD
EASVSELTERANRRAEVLASAPDGDATLSDGERILVLANIGNPSDAPTAKKKGAEGVGLFRTEFLFLDRQDAPTIDEQTE
AYATVLKTFDEGAKVVFRTLDVGADKPLAFADLGAEENPALGRRGLRLSQVRTDLIDAQLEALAKAAEQTGRDPYVMAPM
VATKAEAEWFSSRARAAGIKTVGIMVEVPSTALRSSQVLESVDFASIGTNDLTQYAMAADRLQGELAELLTPWQPAVIQL
VKATCDGAGERLIGVCGEAAGDPLLALVLVGVGVRSLSMAAGKITAVKAALSRHNLEQCRRIADAALAACSPEEARTAAL
KLADPSVEVLVS
>Mature_571_residues
PSLRTCKPTRRPDPMRTLSGLGVSAGVGQAHALVVSPAPGLPASDPSRGAEADLAKVEAALGKVADRLDERSLHADPSAL
AVLQATAMMARDPGLLTSIKSHLSDGHGPAHALWAAFEDFCAQLSAAGGYLAERVTDLRNVRDRAVAVMQGLPEPGVPSF
DSPVILVAEDLAPADTATLNPELVRGLITAAGGPTSHTAILASQIGIPAVVRCSEARDIEDGTPLALDGVTGTVLVEPDE
ASVSELTERANRRAEVLASAPDGDATLSDGERILVLANIGNPSDAPTAKKKGAEGVGLFRTEFLFLDRQDAPTIDEQTEA
YATVLKTFDEGAKVVFRTLDVGADKPLAFADLGAEENPALGRRGLRLSQVRTDLIDAQLEALAKAAEQTGRDPYVMAPMV
ATKAEAEWFSSRARAAGIKTVGIMVEVPSTALRSSQVLESVDFASIGTNDLTQYAMAADRLQGELAELLTPWQPAVIQLV
KATCDGAGERLIGVCGEAAGDPLLALVLVGVGVRSLSMAAGKITAVKAALSRHNLEQCRRIADAALAACSPEEARTAALK
LADPSVEVLVS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=506, Percent_Identity=36.3636363636364, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1788726, Length=543, Percent_Identity=33.5174953959484, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI48994992, Length=536, Percent_Identity=31.9029850746269, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1789193, Length=496, Percent_Identity=30.241935483871, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787994, Length=408, Percent_Identity=29.1666666666667, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59485; Mature: 59354

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLRTCKPTRRPDPMRTLSGLGVSAGVGQAHALVVSPAPGLPASDPSRGAEADLAKVEA
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHH
ALGKVADRLDERSLHADPSALAVLQATAMMARDPGLLTSIKSHLSDGHGPAHALWAAFED
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
FCAQLSAAGGYLAERVTDLRNVRDRAVAVMQGLPEPGVPSFDSPVILVAEDLAPADTATL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCC
NPELVRGLITAAGGPTSHTAILASQIGIPAVVRCSEARDIEDGTPLALDGVTGTVLVEPD
CHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCCEEEEECCC
EASVSELTERANRRAEVLASAPDGDATLSDGERILVLANIGNPSDAPTAKKKGAEGVGLF
CCHHHHHHHHHCCHHHHEEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCEE
RTEFLFLDRQDAPTIDEQTEAYATVLKTFDEGAKVVFRTLDVGADKPLAFADLGAEENPA
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCCHHHCCCCCCCC
LGRRGLRLSQVRTDLIDAQLEALAKAAEQTGRDPYVMAPMVATKAEAEWFSSRARAAGIK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCE
TVGIMVEVPSTALRSSQVLESVDFASIGTNDLTQYAMAADRLQGELAELLTPWQPAVIQL
EEEEEEECCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VKATCDGAGERLIGVCGEAAGDPLLALVLVGVGVRSLSMAAGKITAVKAALSRHNLEQCR
HHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
RIADAALAACSPEEARTAALKLADPSVEVLVS
HHHHHHHHHCCCHHHHHHEEEECCCCEEEEEC
>Mature Secondary Structure 
PSLRTCKPTRRPDPMRTLSGLGVSAGVGQAHALVVSPAPGLPASDPSRGAEADLAKVEA
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHH
ALGKVADRLDERSLHADPSALAVLQATAMMARDPGLLTSIKSHLSDGHGPAHALWAAFED
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
FCAQLSAAGGYLAERVTDLRNVRDRAVAVMQGLPEPGVPSFDSPVILVAEDLAPADTATL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCC
NPELVRGLITAAGGPTSHTAILASQIGIPAVVRCSEARDIEDGTPLALDGVTGTVLVEPD
CHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCCEEEEECCC
EASVSELTERANRRAEVLASAPDGDATLSDGERILVLANIGNPSDAPTAKKKGAEGVGLF
CCHHHHHHHHHCCHHHHEEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCEE
RTEFLFLDRQDAPTIDEQTEAYATVLKTFDEGAKVVFRTLDVGADKPLAFADLGAEENPA
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCCHHHCCCCCCCC
LGRRGLRLSQVRTDLIDAQLEALAKAAEQTGRDPYVMAPMVATKAEAEWFSSRARAAGIK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCE
TVGIMVEVPSTALRSSQVLESVDFASIGTNDLTQYAMAADRLQGELAELLTPWQPAVIQL
EEEEEEECCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VKATCDGAGERLIGVCGEAAGDPLLALVLVGVGVRSLSMAAGKITAVKAALSRHNLEQCR
HHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
RIADAALAACSPEEARTAALKLADPSVEVLVS
HHHHHHHHHCCCHHHHHHEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]