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The map label for this gene is pstS3 [H]

Identifier: 50841827

GI number: 50841827

Start: 391709

End: 392860

Strand: Reverse

Name: pstS3 [H]

Synonym: PPA0341

Alternate gene names: 50841827

Gene position: 392860-391709 (Counterclockwise)

Preceding gene: 50841828

Following gene: 50841826

Centisome position: 15.34

GC content: 60.68

Gene sequence:

>1152_bases
GTGAATCGTTTCGCCAAAATCACTGCCCTGGCTGCTATCAGCGCCATCTCCCTCACCGCTTGTGGTGCCAATGGTGACAA
GGGTTCCAGTTCTGCCTCGTCCTCCAAGACCACCTCGACTGCCAGCTCCGCCGCCCAGCCCAGCGATTCCTCGGCTGACG
CTGTCTCGTTCAAGCCCGCCTGCCCTGGTGGCAGCATCTCCGGTGCTGGTTCCTCGGCTCAGCTGAACGCCATCAACCAA
GTTATTTCCGACTACAAGGCTGCCTGTTCCTCAGCAACCGTCAACTACTCCCCCACCGGCTCCGGCGCTGGCGTGAAGTC
CTTCATCGGTAGGCAAACCGACTGGGCCGGTTCTGACTCCGTCCTCAACGCTGACAAGGGGGAGCCTGATAAGGCCAAGC
AGCGCTGCAACGCCGACCCATGGCACCTCCCGATGGCTGCTGGCCCGATTGCCGTGGTCTACAACGTCGACGGCGTCAAA
GATCTAACCCTCTCCACCGCGACCCTCGCCAAGATTTTCTCCGGCGCCATCACAAACTGGAACGACGACGCCATCAAGAA
GGAGAACCCGAAAGCCGCGTTGCCGTCGGCCCAGATCGAGGTGTTTTACCGCAAGGACGAGTCGGGCACCACCGACAACT
TCACCAAGTTCCTCAACAAGGCGGCCGGTGACATCTGGACTGAGAAGCACTCCAAGACATGGAAAGGTGCGGGCAAGGGC
GCCGATAAGTCGGCCGGCATCGCCCAGGCTGTCAAGAGCACCAAGAACTCGATCTCGTACGACGAGTGGTCCTACGCTAC
AAAGAACAGCCTCGACATGGCCGCCATCGATAACGGAAACGGTCCGGTCAAGCTGACCGGTGAGTCTGCCGGTAAGGCCG
TCTCGGCCGCTAAGGTCGTCGGACAGGGCCATGACCTGCAGCTCGAGCTCCAGTACAAGAACACCCCGGCGGGCGTGTAT
CCGGCCGTTCTCGTAACCTACGAGATCGCTTGTTCCAAGGGCCAGGACTCCGGCAAGGCCGCGCTCTTGAAGGACTTCCT
GTCCTTCTACGCTTCTGAGAGGGAGCAGAAGAAGATCCAGGACCTTGGTTACGCTCCGCTACCCTCCGACGTTGCTTCGA
AGGTTCTGGGTTCCGCCCAGGCCCTCTCCTGA

Upstream 100 bases:

>100_bases
ACCTTGACGCGATCCCTGCGACATTTCCAAACCCTAACGTCATCCCTGTACCAAGGACCGCAACGGCGGCCCCAGTACAT
CACTCGAAGAAGGATGTGCC

Downstream 100 bases:

>100_bases
TTGACTCTCTGCCCGGGGACAAGCGCCCCGGCGCGCCGGGGCGGGAGGACGCCAGTCGTCTTCCCGCCCCTCACCACAAC
ACGACCATGATCGCGACGAA

Product: phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 3; PstS-3 [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNRFAKITALAAISAISLTACGANGDKGSSSASSSKTTSTASSAAQPSDSSADAVSFKPACPGGSISGAGSSAQLNAINQ
VISDYKAACSSATVNYSPTGSGAGVKSFIGRQTDWAGSDSVLNADKGEPDKAKQRCNADPWHLPMAAGPIAVVYNVDGVK
DLTLSTATLAKIFSGAITNWNDDAIKKENPKAALPSAQIEVFYRKDESGTTDNFTKFLNKAAGDIWTEKHSKTWKGAGKG
ADKSAGIAQAVKSTKNSISYDEWSYATKNSLDMAAIDNGNGPVKLTGESAGKAVSAAKVVGQGHDLQLELQYKNTPAGVY
PAVLVTYEIACSKGQDSGKAALLKDFLSFYASEREQKKIQDLGYAPLPSDVASKVLGSAQALS

Sequences:

>Translated_383_residues
MNRFAKITALAAISAISLTACGANGDKGSSSASSSKTTSTASSAAQPSDSSADAVSFKPACPGGSISGAGSSAQLNAINQ
VISDYKAACSSATVNYSPTGSGAGVKSFIGRQTDWAGSDSVLNADKGEPDKAKQRCNADPWHLPMAAGPIAVVYNVDGVK
DLTLSTATLAKIFSGAITNWNDDAIKKENPKAALPSAQIEVFYRKDESGTTDNFTKFLNKAAGDIWTEKHSKTWKGAGKG
ADKSAGIAQAVKSTKNSISYDEWSYATKNSLDMAAIDNGNGPVKLTGESAGKAVSAAKVVGQGHDLQLELQYKNTPAGVY
PAVLVTYEIACSKGQDSGKAALLKDFLSFYASEREQKKIQDLGYAPLPSDVASKVLGSAQALS
>Mature_383_residues
MNRFAKITALAAISAISLTACGANGDKGSSSASSSKTTSTASSAAQPSDSSADAVSFKPACPGGSISGAGSSAQLNAINQ
VISDYKAACSSATVNYSPTGSGAGVKSFIGRQTDWAGSDSVLNADKGEPDKAKQRCNADPWHLPMAAGPIAVVYNVDGVK
DLTLSTATLAKIFSGAITNWNDDAIKKENPKAALPSAQIEVFYRKDESGTTDNFTKFLNKAAGDIWTEKHSKTWKGAGKG
ADKSAGIAQAVKSTKNSISYDEWSYATKNSLDMAAIDNGNGPVKLTGESAGKAVSAAKVVGQGHDLQLELQYKNTPAGVY
PAVLVTYEIACSKGQDSGKAALLKDFLSFYASEREQKKIQDLGYAPLPSDVASKVLGSAQALS

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=323, Percent_Identity=29.7213622291022, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39547; Mature: 39547

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFAKITALAAISAISLTACGANGDKGSSSASSSKTTSTASSAAQPSDSSADAVSFKPA
CCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCC
CPGGSISGAGSSAQLNAINQVISDYKAACSSATVNYSPTGSGAGVKSFIGRQTDWAGSDS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCCCCCCCCCC
VLNADKGEPDKAKQRCNADPWHLPMAAGPIAVVYNVDGVKDLTLSTATLAKIFSGAITNW
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
NDDAIKKENPKAALPSAQIEVFYRKDESGTTDNFTKFLNKAAGDIWTEKHSKTWKGAGKG
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADKSAGIAQAVKSTKNSISYDEWSYATKNSLDMAAIDNGNGPVKLTGESAGKAVSAAKVV
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHH
GQGHDLQLELQYKNTPAGVYPAVLVTYEIACSKGQDSGKAALLKDFLSFYASEREQKKIQ
CCCCCEEEEEEECCCCCCHHHHHHHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DLGYAPLPSDVASKVLGSAQALS
HCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRFAKITALAAISAISLTACGANGDKGSSSASSSKTTSTASSAAQPSDSSADAVSFKPA
CCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCC
CPGGSISGAGSSAQLNAINQVISDYKAACSSATVNYSPTGSGAGVKSFIGRQTDWAGSDS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCCCCCCCCCC
VLNADKGEPDKAKQRCNADPWHLPMAAGPIAVVYNVDGVKDLTLSTATLAKIFSGAITNW
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
NDDAIKKENPKAALPSAQIEVFYRKDESGTTDNFTKFLNKAAGDIWTEKHSKTWKGAGKG
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADKSAGIAQAVKSTKNSISYDEWSYATKNSLDMAAIDNGNGPVKLTGESAGKAVSAAKVV
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHH
GQGHDLQLELQYKNTPAGVYPAVLVTYEIACSKGQDSGKAALLKDFLSFYASEREQKKIQ
CCCCCEEEEEEECCCCCCHHHHHHHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DLGYAPLPSDVASKVLGSAQALS
HCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA