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The map label for this gene is nhaA

Identifier: 50841720

GI number: 50841720

Start: 280063

End: 281325

Strand: Reverse

Name: nhaA

Synonym: PPA0227

Alternate gene names: 50841720

Gene position: 281325-280063 (Counterclockwise)

Preceding gene: 50841721

Following gene: 50841719

Centisome position: 10.99

GC content: 60.1

Gene sequence:

>1263_bases
ATGACGGATTCCCGCAAGAACAAGCGGCCTCGCAAGAGCATCCTTCGACCGGTGACAGGCCCGAACGCCCTTCACCTGTC
TGACATCTTCCGAAACGAAACCACTGGCGGCATGCTGATGCTGGCAGCCACGGTCGCAGCCCTCTTGTGGGCCAATCTTG
GTCATCACTCGTACCATTTCTTCCGTGAATTGGCTCTTGGTCCGCTCACAATCGAGCAGTGGGCGGCTGACGGCCTTCTA
ACGGTCTTCTTCTTCATCGCCGGCCTCGAACTCAAGCGAGAATTCGTTGAGGGATCGCTATCGCGACCTGCTGACGCATT
GGTCCCCATCGTTGCCGCAGTATGCGGAATGGTGTTCCCGGCTGGCATCTACACGCTATTCAACGTGTTAGCCAGCGACG
GACACCCGGCAGGTTGGGCCATCCCGATGGCAACCGACATCGCCTTCGCCTTGGCGGTACTAGCCATCGTCGGAGCAGGC
TTGCCACAGGCCGTACGAGCCTTCCTGCTCACCTTGGCCATTGCCGATGACCTCGGTTCGATCATCGTGATCGCAGTCTT
TTTCTCCACGGGCCTGGACATCTGGTGGCTCGCCGGTGCGATCGCCTGCATCGGCCTCTGGGGAGTGATGCAACACTTCC
ACGTCGATAACGGCTGGTGGTACGTGCCGATCTTCATCGTCGGGTGGTGGTGCATGCTGCGCTCCGGCGTCCACGCCACC
ATCGCCGGCGTCGCTTTTGGCCTCCTGACCCGCACTGAGGAAGACGTCCTGGACGACCCCGTTGATCGCTGGCAGCACAA
AGTGGAGCCATGGTCGGCCGGTGTCGTCGTCCCCTTTTTCGCTCTCATGAGCGCCGGTGTCCACGTCAGCGGGAAAACCT
TCCTGGCCCTATGGACTCACCCAATCTCGCTGGGTATCGTCTGCGGGCTCATTCTTGGAAAGGTCATCGGCATCACCCTC
GGATCCTGTCTCACCGCCCGATTCACCTCGGCTGAGCTGGGTCGCGGTGTTGTATGGCGCGACATCATCGCGGTAGCTGT
GTTGGCGGGCATCGGATTTACCGTCTCAATGCTCATGACCGATCTATCCTTCCCAAAGAACCACGAATTCGCGGACGAGG
CCAAGGCATCCGTGCTGATGGCATCCTTCTTGGCGGCCATCCTTGGTGGTGCCATGCTGCACCATCGCGGCAAAAGTCAC
GCCATGCGCCAGACCAAGCATTCCCACGAGGTTCCGGTTGCAGCCGGTACCTGCAACGACTAA

Upstream 100 bases:

>100_bases
ATTTGGTATGCTGCTACACAGGGTGTGCCGGGAAGTCTGGTCGGCGTGACTCTGTCCCCTATCCCGCTGAGCCCGCGCCG
CGGGTTCCCGAGAGGCATTT

Downstream 100 bases:

>100_bases
AGCCAGTTCCTGCGACGACAACTCAACCCGAACACAGCAGATCGAACCTATTCCCGTTAGGCTGAGATCATTACATTCAT
CACGCGGAGGCATCCCATGG

Product: putative sodium:proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTDSRKNKRPRKSILRPVTGPNALHLSDIFRNETTGGMLMLAATVAALLWANLGHHSYHFFRELALGPLTIEQWAADGLL
TVFFFIAGLELKREFVEGSLSRPADALVPIVAAVCGMVFPAGIYTLFNVLASDGHPAGWAIPMATDIAFALAVLAIVGAG
LPQAVRAFLLTLAIADDLGSIIVIAVFFSTGLDIWWLAGAIACIGLWGVMQHFHVDNGWWYVPIFIVGWWCMLRSGVHAT
IAGVAFGLLTRTEEDVLDDPVDRWQHKVEPWSAGVVVPFFALMSAGVHVSGKTFLALWTHPISLGIVCGLILGKVIGITL
GSCLTARFTSAELGRGVVWRDIIAVAVLAGIGFTVSMLMTDLSFPKNHEFADEAKASVLMASFLAAILGGAMLHHRGKSH
AMRQTKHSHEVPVAAGTCND

Sequences:

>Translated_420_residues
MTDSRKNKRPRKSILRPVTGPNALHLSDIFRNETTGGMLMLAATVAALLWANLGHHSYHFFRELALGPLTIEQWAADGLL
TVFFFIAGLELKREFVEGSLSRPADALVPIVAAVCGMVFPAGIYTLFNVLASDGHPAGWAIPMATDIAFALAVLAIVGAG
LPQAVRAFLLTLAIADDLGSIIVIAVFFSTGLDIWWLAGAIACIGLWGVMQHFHVDNGWWYVPIFIVGWWCMLRSGVHAT
IAGVAFGLLTRTEEDVLDDPVDRWQHKVEPWSAGVVVPFFALMSAGVHVSGKTFLALWTHPISLGIVCGLILGKVIGITL
GSCLTARFTSAELGRGVVWRDIIAVAVLAGIGFTVSMLMTDLSFPKNHEFADEAKASVLMASFLAAILGGAMLHHRGKSH
AMRQTKHSHEVPVAAGTCND
>Mature_419_residues
TDSRKNKRPRKSILRPVTGPNALHLSDIFRNETTGGMLMLAATVAALLWANLGHHSYHFFRELALGPLTIEQWAADGLLT
VFFFIAGLELKREFVEGSLSRPADALVPIVAAVCGMVFPAGIYTLFNVLASDGHPAGWAIPMATDIAFALAVLAIVGAGL
PQAVRAFLLTLAIADDLGSIIVIAVFFSTGLDIWWLAGAIACIGLWGVMQHFHVDNGWWYVPIFIVGWWCMLRSGVHATI
AGVAFGLLTRTEEDVLDDPVDRWQHKVEPWSAGVVVPFFALMSAGVHVSGKTFLALWTHPISLGIVCGLILGKVIGITLG
SCLTARFTSAELGRGVVWRDIIAVAVLAGIGFTVSMLMTDLSFPKNHEFADEAKASVLMASFLAAILGGAMLHHRGKSHA
MRQTKHSHEVPVAAGTCND

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=383, Percent_Identity=39.1644908616188, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_PROAC (Q6AB77)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_054947.1
- HSSP:   P13738
- ProteinModelPortal:   Q6AB77
- SMR:   Q6AB77
- GeneID:   2931694
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0227
- NMPDR:   fig|267747.1.peg.224
- HOGENOM:   HBG665619
- OMA:   YFALRDL
- ProtClustDB:   CLSK2763142
- BioCyc:   PACN267747:PPA0227-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 45164; Mature: 45033

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x114df8f4)-; HASH(0x10821070)-; HASH(0x10da8db4)-; HASH(0x10310278)-; HASH(0x118f47f0)-; HASH(0x1103ae68)-; HASH(0x118f234c)-; HASH(0x1157ffb4)-; HASH(0x11381e28)-; HASH(0x10b250bc)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSRKNKRPRKSILRPVTGPNALHLSDIFRNETTGGMLMLAATVAALLWANLGHHSYHF
CCCCCCCCCCHHHHHCCCCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
FRELALGPLTIEQWAADGLLTVFFFIAGLELKREFVEGSLSRPADALVPIVAAVCGMVFP
HHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AGIYTLFNVLASDGHPAGWAIPMATDIAFALAVLAIVGAGLPQAVRAFLLTLAIADDLGS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IIVIAVFFSTGLDIWWLAGAIACIGLWGVMQHFHVDNGWWYVPIFIVGWWCMLRSGVHAT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHH
IAGVAFGLLTRTEEDVLDDPVDRWQHKVEPWSAGVVVPFFALMSAGVHVSGKTFLALWTH
HHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCCEEEEEHHH
PISLGIVCGLILGKVIGITLGSCLTARFTSAELGRGVVWRDIIAVAVLAGIGFTVSMLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLSFPKNHEFADEAKASVLMASFLAAILGGAMLHHRGKSHAMRQTKHSHEVPVAAGTCND
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure 
TDSRKNKRPRKSILRPVTGPNALHLSDIFRNETTGGMLMLAATVAALLWANLGHHSYHF
CCCCCCCCCHHHHHCCCCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
FRELALGPLTIEQWAADGLLTVFFFIAGLELKREFVEGSLSRPADALVPIVAAVCGMVFP
HHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AGIYTLFNVLASDGHPAGWAIPMATDIAFALAVLAIVGAGLPQAVRAFLLTLAIADDLGS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IIVIAVFFSTGLDIWWLAGAIACIGLWGVMQHFHVDNGWWYVPIFIVGWWCMLRSGVHAT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHH
IAGVAFGLLTRTEEDVLDDPVDRWQHKVEPWSAGVVVPFFALMSAGVHVSGKTFLALWTH
HHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCCEEEEEHHH
PISLGIVCGLILGKVIGITLGSCLTARFTSAELGRGVVWRDIIAVAVLAGIGFTVSMLMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DLSFPKNHEFADEAKASVLMASFLAAILGGAMLHHRGKSHAMRQTKHSHEVPVAAGTCND
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA