Click here to switch to the map view.

The map label for this gene is pbpA [H]

Identifier: 50841674

GI number: 50841674

Start: 225800

End: 227227

Strand: Reverse

Name: pbpA [H]

Synonym: PPA0185

Alternate gene names: 50841674

Gene position: 227227-225800 (Counterclockwise)

Preceding gene: 50841675

Following gene: 50841673

Centisome position: 8.88

GC content: 59.1

Gene sequence:

>1428_bases
ATGAATAGACAAATTCGCGCAGTCTCTTTGCTCGCCGGGCTGATGTTCCTGGCCCTCATGGTCAACCTCACAGGTTCGGC
TATGTTCCGGCAGGCCAGTCTCAACAACGATCCCCACAACGTCCGGGTGCGTGACGCGGAGTTCTCCCAGAACCGCGGCA
ACATCCTCGTCGGTTCGAGACCGATCGCTACCACGACGTCAAGCAATGGCAAGTTCGCCTATCAGCGTGTGTACCTATCG
GGGCCGAAGTACGCACCCATCACCGGGTATTACTCCTACTACTACGGACGATCCATGCTGGAGCAGACCCAAAACGCCCA
GCTCACCGGAACCTCCGATGCCCAATGGCTCTCTCGCATCACCGGCACCTTATCTGGCCACAAACCTGAGGGCGGCTCTA
TCACCACGACGATTAACGCCAAAGCACAGGATGCCGCGTGGAACGGGCTCAAGGGAAAGAAGGGCGCTGTCGTCGCATTG
GACTACACCACTGGCGCGGTGCTGGCTATGGCGTCGTCCCCCTCCTATGATCCCAACGAGCTTGCCTCCCACCACCTCAA
CGACACCACCCGAGCATGGAAGAACCTCGTCGCGGACCCGTCGTCTCCGCTGTCGAACCGCGCGACCCGAGAAATATATC
CTCCGGGCTCAACATTCAAACTGGTGACCGCCGCGGCAGCTCTCCAGAACGGGTATCACCCGAATTCCATGGTCGACTCA
CCGGAAAATTGGATCCTGCCGGGCACTCGCACCCCGCTGACGAACGAGACCAACTGTGGGGGCAGCAGGATCACCTTGAC
CCACGCCCTTGACATCTCGTGCAATACCGCCTTCGGTAAAGTTGGCGTGAGCCTGGGCCAAGACAAGATCCGAGATCAGG
CTGAGCGATTCGGGTTTGGCAAGGTTGTCAACTCCGATGTGTCCTCAGTCGCATCAAGATTCCCCCAGAACCTCACCGAT
GCGCAGTTGGCGCAGAGCTCCATCGGCCAGTTCGACGTCGCAGCCTCTCCGCTGCAAATGGCCATGGTGACTGCCGGCAT
CGCCAATGACGGCAAACTCATGACCCCTTACCTCACCGCGCAGGTGAGAGCATCAAACCTGCAGGTAGTCTCCGAACATC
ACCCGAAGCAGATGTCTCAACCGATGACAAAGGAGTCGGCCGAGCAGCTAAAGGCGATGATGGTCTCGGTGGTCAACAAT
GGCACTGGCAAGAGGGCGCGCATTAATGGGACCACCGTCGGCGGTAAGACCGGCACCGCCCAGACGGTACAAGGTAAGGC
CCCCTATGCGTGGTTCGTCGGATGGTCGGACAACCCGCACGTCGCAGTAGCGGTCTTCATCCAATCTTTGGACACCGCCA
TCAATGAAATCTCGGGAGGCCGACTCGCGGCTCCCATCGCTAGAGATGTCATCGAGGCGATGAGATGA

Upstream 100 bases:

>100_bases
TCATCATGATCGTCTCACACCGGAACCGAAAGCCTGCCGACGACTTCGCCGCCACCGTCCCGGCACCTGATCCGCAACAG
GCCATCACCGGAGGCACGCG

Downstream 100 bases:

>100_bases
CAAGCAGACAAACAACCCACTACGTCAACACAAAGGAGATCCCTCAATGAGCGCTGAGAGTTGGCTCTCCGGGCGTTACG
AGCTGCAGAATCTCATCGGT

Product: putative penicillin-binding protein

Products: NA

Alternate protein names: PBPA [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNRQIRAVSLLAGLMFLALMVNLTGSAMFRQASLNNDPHNVRVRDAEFSQNRGNILVGSRPIATTTSSNGKFAYQRVYLS
GPKYAPITGYYSYYYGRSMLEQTQNAQLTGTSDAQWLSRITGTLSGHKPEGGSITTTINAKAQDAAWNGLKGKKGAVVAL
DYTTGAVLAMASSPSYDPNELASHHLNDTTRAWKNLVADPSSPLSNRATREIYPPGSTFKLVTAAAALQNGYHPNSMVDS
PENWILPGTRTPLTNETNCGGSRITLTHALDISCNTAFGKVGVSLGQDKIRDQAERFGFGKVVNSDVSSVASRFPQNLTD
AQLAQSSIGQFDVAASPLQMAMVTAGIANDGKLMTPYLTAQVRASNLQVVSEHHPKQMSQPMTKESAEQLKAMMVSVVNN
GTGKRARINGTTVGGKTGTAQTVQGKAPYAWFVGWSDNPHVAVAVFIQSLDTAINEISGGRLAAPIARDVIEAMR

Sequences:

>Translated_475_residues
MNRQIRAVSLLAGLMFLALMVNLTGSAMFRQASLNNDPHNVRVRDAEFSQNRGNILVGSRPIATTTSSNGKFAYQRVYLS
GPKYAPITGYYSYYYGRSMLEQTQNAQLTGTSDAQWLSRITGTLSGHKPEGGSITTTINAKAQDAAWNGLKGKKGAVVAL
DYTTGAVLAMASSPSYDPNELASHHLNDTTRAWKNLVADPSSPLSNRATREIYPPGSTFKLVTAAAALQNGYHPNSMVDS
PENWILPGTRTPLTNETNCGGSRITLTHALDISCNTAFGKVGVSLGQDKIRDQAERFGFGKVVNSDVSSVASRFPQNLTD
AQLAQSSIGQFDVAASPLQMAMVTAGIANDGKLMTPYLTAQVRASNLQVVSEHHPKQMSQPMTKESAEQLKAMMVSVVNN
GTGKRARINGTTVGGKTGTAQTVQGKAPYAWFVGWSDNPHVAVAVFIQSLDTAINEISGGRLAAPIARDVIEAMR
>Mature_475_residues
MNRQIRAVSLLAGLMFLALMVNLTGSAMFRQASLNNDPHNVRVRDAEFSQNRGNILVGSRPIATTTSSNGKFAYQRVYLS
GPKYAPITGYYSYYYGRSMLEQTQNAQLTGTSDAQWLSRITGTLSGHKPEGGSITTTINAKAQDAAWNGLKGKKGAVVAL
DYTTGAVLAMASSPSYDPNELASHHLNDTTRAWKNLVADPSSPLSNRATREIYPPGSTFKLVTAAAALQNGYHPNSMVDS
PENWILPGTRTPLTNETNCGGSRITLTHALDISCNTAFGKVGVSLGQDKIRDQAERFGFGKVVNSDVSSVASRFPQNLTD
AQLAQSSIGQFDVAASPLQMAMVTAGIANDGKLMTPYLTAQVRASNLQVVSEHHPKQMSQPMTKESAEQLKAMMVSVVNN
GTGKRARINGTTVGGKTGTAQTVQGKAPYAWFVGWSDNPHVAVAVFIQSLDTAINEISGGRLAAPIARDVIEAMR

Specific function: Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Probable). Note=Localized to the division site (septum) along with newly synthesized peptidoglycan, between segregated nucleoids (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=327, Percent_Identity=30.5810397553517, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1786272, Length=328, Percent_Identity=28.6585365853659, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 50833; Mature: 50833

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRQIRAVSLLAGLMFLALMVNLTGSAMFRQASLNNDPHNVRVRDAEFSQNRGNILVGSR
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCC
PIATTTSSNGKFAYQRVYLSGPKYAPITGYYSYYYGRSMLEQTQNAQLTGTSDAQWLSRI
CEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHH
TGTLSGHKPEGGSITTTINAKAQDAAWNGLKGKKGAVVALDYTTGAVLAMASSPSYDPNE
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHH
LASHHLNDTTRAWKNLVADPSSPLSNRATREIYPPGSTFKLVTAAAALQNGYHPNSMVDS
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
PENWILPGTRTPLTNETNCGGSRITLTHALDISCNTAFGKVGVSLGQDKIRDQAERFGFG
CCCEECCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHCCCH
KVVNSDVSSVASRFPQNLTDAQLAQSSIGQFDVAASPLQMAMVTAGIANDGKLMTPYLTA
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEECCEEEE
QVRASNLQVVSEHHPKQMSQPMTKESAEQLKAMMVSVVNNGTGKRARINGTTVGGKTGTA
EEECCCEEEHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCC
QTVQGKAPYAWFVGWSDNPHVAVAVFIQSLDTAINEISGGRLAAPIARDVIEAMR
CEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MNRQIRAVSLLAGLMFLALMVNLTGSAMFRQASLNNDPHNVRVRDAEFSQNRGNILVGSR
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCC
PIATTTSSNGKFAYQRVYLSGPKYAPITGYYSYYYGRSMLEQTQNAQLTGTSDAQWLSRI
CEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHH
TGTLSGHKPEGGSITTTINAKAQDAAWNGLKGKKGAVVALDYTTGAVLAMASSPSYDPNE
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHH
LASHHLNDTTRAWKNLVADPSSPLSNRATREIYPPGSTFKLVTAAAALQNGYHPNSMVDS
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
PENWILPGTRTPLTNETNCGGSRITLTHALDISCNTAFGKVGVSLGQDKIRDQAERFGFG
CCCEECCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHCCCH
KVVNSDVSSVASRFPQNLTDAQLAQSSIGQFDVAASPLQMAMVTAGIANDGKLMTPYLTA
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEECCEEEE
QVRASNLQVVSEHHPKQMSQPMTKESAEQLKAMMVSVVNNGTGKRARINGTTVGGKTGTA
EEECCCEEEHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCC
QTVQGKAPYAWFVGWSDNPHVAVAVFIQSLDTAINEISGGRLAAPIARDVIEAMR
CEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA