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The map label for this gene is sacP [H]

Identifier: 50365333

GI number: 50365333

Start: 607873

End: 609591

Strand: Reverse

Name: sacP [H]

Synonym: Mfl516

Alternate gene names: 50365333

Gene position: 609591-607873 (Counterclockwise)

Preceding gene: 50365334

Following gene: 50365332

Centisome position: 76.85

GC content: 30.54

Gene sequence:

>1719_bases
ATGAAAAAGAAAGATTATGCGTTTGAAGCCAAGCGTTTTATCAAAGCAGTTGGAGGTAAGTCAAACATTGAGTCTTACCT
TCACTGTGTTACAAGATTAAGATTAAATCTTATTGATAAAACAAAAGTTAATGAAGCTGAAATAAAAGCCTCTCCATTAA
CAAAAGGAATAAACTATTCACAAAATCAACTACAAATTATTTTAGGTAGTGGTGTTGTTGAAGCTGTTTATGAAGAAGTT
CAAAAAGAAATGGAAGTAAGTAATTTATCACTAGATGAAAAATTGAGCAATGATAAATTAGAAGACTTTAAATTACAAGC
TAAAGCCAATAAAGAAGCTTTAAGAAATAAATCAGGATTTTTTGGACAAATCCAAAAAGGTATGAGAGTTTTAGGAGACA
TATTTGTTCCTATCATTCCTGCTATTGTAGCGGCTGGATTAGCAATGGGTGTGGCTGCATTATTAAAAAGAATTTTAAAC
CCATCAGGAGCTGAACTTAATTCATTATTATGAATTATTGTTGATATTATTACTAAAACAGCATTTGACTCATTAGCAGT
TTTAGTTTGTTGATCAACTGTTAAAAGATTTGGTGGAAATCCTGTATTAGGTATTATTATTGGATTAATGTTAGTTAGTC
CGTTATTGCCAAACAAAGGATCAATTGCAGCATACAATGCACAATTAGATTTCTTTAATGATCAATCAAGTAAAGGTTTA
AATATAAATCAAATATATGATTATTGAAATAATGCAGGTTTAAAATGAATGGGAACTGCTCCAATAATTGATGATGAGTC
AAAAGAAATTTTAGTTAATGCAACAATGGGTGCGCCAATAACAGCTATAAAATTTTGAATAATACCTATTACAGGTTACC
AAGGTTCAGTTTTACCAGCTTTAATAATAGGTATTGTAGTAGCATATTTAGAAAAATGAATTAAATCATGAATGCCTAAA
TCAGTAAATATGATTTTTACTCCATTCTTAACCATTTCATTTGCTTTATTAATTGCGTTATTTTTCTTTGGACCAATTTT
ATTAATGGTTGAACAAGGAATATTAATTGCAACAAAAGCAATATTAGGAATTAAATACGGATTTGGAACAGCTATTATTG
CAGGTCTTTTACAAGGTATTGTTATTACTGGTTGTCACCAAGTATTACAAGGACTTGAAATGCAACTTGTTATTGATGGA
AGTTTACCAGGAGCTAACGGGGCAGACCCTGCGGGTTCAATATTTAATGCAATATGAACAGCATCAATTATTTCACAGGG
TGGAGCTGCAATGGCGATAGCTATAAAAGCTAAAGACAAATCAGATAAAGAATTAGGTTTTTCTGCAGCACTTTCAACAT
TCTTTGGAATTACAGAACCTGCTATATTTGGTACAAACTTGCCTAAAGTAAAACCATTTATATATGCATCTATTGGTGCA
TTCTTTGGTGGTCTATTTGCAGGAGCATTGAATGTTACATGTCCAGGAATGGGAGTAACTGTAGTACCTGGATTATTACT
TTATACAGGAGATTGAGTTAAATTATTGGGAATTATAGGTGTTAACATTATAGCCTTTAGTTCAGCATTCTTATTAACAT
TCTTCTTATTTAAAGAAGGAGTAGAAGCTAAAAAAACCAAATTAGGTTTAAAATTTGATGAGTTAAAAGCAAAAGTAAAA
TTTGACAAAATCAAATTTAGAAAAAACAAAGCGGAGTAA

Upstream 100 bases:

>100_bases
TTTGGAGAACTACTTGAATATAGATTAAGTAGGTATAAGCCTAAATTTTTCAAATGAGATTTTATAGAGTGAAGAAAATA
AAAATATAAAAGAGGAAAAA

Downstream 100 bases:

>100_bases
TGACATGCAAAAAGAAAAATATAGCTTAATAAGCAATAATGATCTTGAAGATATTCAAAAATGACATGATAAAAAACAAA
GCGATTGATACAATAATCAG

Product: sucrose PTS system IIBC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKKKDYAFEAKRFIKAVGGKSNIESYLHCVTRLRLNLIDKTKVNEAEIKASPLTKGINYSQNQLQIILGSGVVEAVYEEV
QKEMEVSNLSLDEKLSNDKLEDFKLQAKANKEALRNKSGFFGQIQKGMRVLGDIFVPIIPAIVAAGLAMGVAALLKRILN
PSGAELNSLLWIIVDIITKTAFDSLAVLVCWSTVKRFGGNPVLGIIIGLMLVSPLLPNKGSIAAYNAQLDFFNDQSSKGL
NINQIYDYWNNAGLKWMGTAPIIDDESKEILVNATMGAPITAIKFWIIPITGYQGSVLPALIIGIVVAYLEKWIKSWMPK
SVNMIFTPFLTISFALLIALFFFGPILLMVEQGILIATKAILGIKYGFGTAIIAGLLQGIVITGCHQVLQGLEMQLVIDG
SLPGANGADPAGSIFNAIWTASIISQGGAAMAIAIKAKDKSDKELGFSAALSTFFGITEPAIFGTNLPKVKPFIYASIGA
FFGGLFAGALNVTCPGMGVTVVPGLLLYTGDWVKLLGIIGVNIIAFSSAFLLTFFLFKEGVEAKKTKLGLKFDELKAKVK
FDKIKFRKNKAE

Sequences:

>Translated_572_residues
MKKKDYAFEAKRFIKAVGGKSNIESYLHCVTRLRLNLIDKTKVNEAEIKASPLTKGINYSQNQLQIILGSGVVEAVYEEV
QKEMEVSNLSLDEKLSNDKLEDFKLQAKANKEALRNKSGFFGQIQKGMRVLGDIFVPIIPAIVAAGLAMGVAALLKRILN
PSGAELNSLL*IIVDIITKTAFDSLAVLVC*STVKRFGGNPVLGIIIGLMLVSPLLPNKGSIAAYNAQLDFFNDQSSKGL
NINQIYDY*NNAGLK*MGTAPIIDDESKEILVNATMGAPITAIKF*IIPITGYQGSVLPALIIGIVVAYLEK*IKS*MPK
SVNMIFTPFLTISFALLIALFFFGPILLMVEQGILIATKAILGIKYGFGTAIIAGLLQGIVITGCHQVLQGLEMQLVIDG
SLPGANGADPAGSIFNAI*TASIISQGGAAMAIAIKAKDKSDKELGFSAALSTFFGITEPAIFGTNLPKVKPFIYASIGA
FFGGLFAGALNVTCPGMGVTVVPGLLLYTGD*VKLLGIIGVNIIAFSSAFLLTFFLFKEGVEAKKTKLGLKFDELKAKVK
FDKIKFRKNKAE
>Mature_572_residues
MKKKDYAFEAKRFIKAVGGKSNIESYLHCVTRLRLNLIDKTKVNEAEIKASPLTKGINYSQNQLQIILGSGVVEAVYEEV
QKEMEVSNLSLDEKLSNDKLEDFKLQAKANKEALRNKSGFFGQIQKGMRVLGDIFVPIIPAIVAAGLAMGVAALLKRILN
PSGAELNSLL*IIVDIITKTAFDSLAVLVC*STVKRFGGNPVLGIIIGLMLVSPLLPNKGSIAAYNAQLDFFNDQSSKGL
NINQIYDY*NNAGLK*MGTAPIIDDESKEILVNATMGAPITAIKF*IIPITGYQGSVLPALIIGIVVAYLEK*IKS*MPK
SVNMIFTPFLTISFALLIALFFFGPILLMVEQGILIATKAILGIKYGFGTAIIAGLLQGIVITGCHQVLQGLEMQLVIDG
SLPGANGADPAGSIFNAI*TASIISQGGAAMAIAIKAKDKSDKELGFSAALSTFFGITEPAIFGTNLPKVKPFIYASIGA
FFGGLFAGALNVTCPGMGVTVVPGLLLYTGD*VKLLGIIGVNIIAFSSAFLLTFFLFKEGVEAKKTKLGLKFDELKAKVK
FDKIKFRKNKAE

Specific function: Acts as both a kinase and a phosphatase on sacT [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=509, Percent_Identity=27.3084479371316, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1790159, Length=481, Percent_Identity=25.987525987526, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI48994906, Length=545, Percent_Identity=24.954128440367, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788769, Length=221, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60043; Mature: 60043

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKDYAFEAKRFIKAVGGKSNIESYLHCVTRLRLNLIDKTKVNEAEIKASPLTKGINYS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCC
QNQLQIILGSGVVEAVYEEVQKEMEVSNLSLDEKLSNDKLEDFKLQAKANKEALRNKSGF
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHEEHHHCCHHHHHCCCCC
FGQIQKGMRVLGDIFVPIIPAIVAAGLAMGVAALLKRILNPSGAELNSLLIIVDIITKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FDSLAVLVCSTVKRFGGNPVLGIIIGLMLVSPLLPNKGSIAAYNAQLDFFNDQSSKGLNI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCC
NQIYDYNNAGLKMGTAPIIDDESKEILVNATMGAPITAIKFIIPITGYQGSVLPALIIGI
CEEEECCCCCCEECCCCCCCCCCCEEEEEECCCCCHHHEEEEEEECCCCCCHHHHHHHHH
VVAYLEKIKSMPKSVNMIFTPFLTISFALLIALFFFGPILLMVEQGILIATKAILGIKYG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
FGTAIIAGLLQGIVITGCHQVLQGLEMQLVIDGSLPGANGADPAGSIFNAITASIISQGG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
AAMAIAIKAKDKSDKELGFSAALSTFFGITEPAIFGTNLPKVKPFIYASIGAFFGGLFAG
CEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALNVTCPGMGVTVVPGLLLYTGDVKLLGIIGVNIIAFSSAFLLTFFLFKEGVEAKKTKLG
HHHEECCCCCHHHHCCHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
LKFDELKAKVKFDKIKFRKNKAE
CCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKKDYAFEAKRFIKAVGGKSNIESYLHCVTRLRLNLIDKTKVNEAEIKASPLTKGINYS
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCC
QNQLQIILGSGVVEAVYEEVQKEMEVSNLSLDEKLSNDKLEDFKLQAKANKEALRNKSGF
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHEEHHHCCHHHHHCCCCC
FGQIQKGMRVLGDIFVPIIPAIVAAGLAMGVAALLKRILNPSGAELNSLLIIVDIITKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FDSLAVLVCSTVKRFGGNPVLGIIIGLMLVSPLLPNKGSIAAYNAQLDFFNDQSSKGLNI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCC
NQIYDYNNAGLKMGTAPIIDDESKEILVNATMGAPITAIKFIIPITGYQGSVLPALIIGI
CEEEECCCCCCEECCCCCCCCCCCEEEEEECCCCCHHHEEEEEEECCCCCCHHHHHHHHH
VVAYLEKIKSMPKSVNMIFTPFLTISFALLIALFFFGPILLMVEQGILIATKAILGIKYG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
FGTAIIAGLLQGIVITGCHQVLQGLEMQLVIDGSLPGANGADPAGSIFNAITASIISQGG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
AAMAIAIKAKDKSDKELGFSAALSTFFGITEPAIFGTNLPKVKPFIYASIGAFFGGLFAG
CEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALNVTCPGMGVTVVPGLLLYTGDVKLLGIIGVNIIAFSSAFLLTFFLFKEGVEAKKTKLG
HHHEECCCCCHHHHCCHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
LKFDELKAKVKFDKIKFRKNKAE
CCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3122206; 7934828; 9384377; 10568751 [H]