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The map label for this gene is scrB [H]

Identifier: 50365332

GI number: 50365332

Start: 606417

End: 607868

Strand: Reverse

Name: scrB [H]

Synonym: Mfl515

Alternate gene names: 50365332

Gene position: 607868-606417 (Counterclockwise)

Preceding gene: 50365333

Following gene: 50365331

Centisome position: 76.63

GC content: 24.24

Gene sequence:

>1452_bases
ATGCAAAAAGAAAAATATAGCTTAATAAGCAATAATGATCTTGAAGATATTCAAAAATGACATGATAAAAAACAAAGCGA
TTGATACAATAATCAGTTTCATTTAGCTGGATATTCAGGTTCTACTAATGACCCTAATGGATTAACATACCATAAAGGAA
AATATTATATCTTTATGCAAAGCTGCCCATTTAGTATTCAACATTTTAATAAATCATGAGCATTATATACAACAACTGAT
TTTATTAATTACAACTATGAAGGTTTAACTTTAATACCTTCAACAAAATATGATATTAATGGAGTTTTTTCAGGAAGCGC
TAGAATAAATGAAAATGATGAAATTGAAATATACTATACAGGTAATATAAAGTTTAATGATGTTGATAGAACAGCATATA
CATTAAAAGCATTTATAGATTTAAAAGAAAAATTAGTAACAAAAGAATTACTTTTTGAAGCAGATTTAACTAAATATACA
GGTCACTATAGAGATCCAATTGTTTTTGAAAAAAATAATCAATTGTATATGCTTAACGGAGCTCAAACAAAAGATTTAAA
AGCAATGCTAAATGTTTATAGATTTAATGGATCAAATTGAGAAAATTTCAAAGATATTAAATTTGATGAAGCTGATGAAC
AAAATGCTTACATGCTAGAATGTCCTAATTATTTTAAATTGGATGGAAGAGAATATGTTTTCGCATGTTTAGAACAAGAT
GCACCATTAAAAGATGGAAGTCACTATGTTAAATATAGAGAAGTTGAAATTGATAATGATATAAATTTCACTTATAAAAC
TAGTTTATTAAAAATTGATTTAGGTTTTGACTTTTATGCCCCACAAGTATTTAGTAATACTGGAGAAAGAGTAATTATGT
TAGGATGATTAGGTAACTCAAAATCCAATCCATTCCCACCAGAGCTTACAACATGAAGTAATCAACTAACAGTTCCAAGA
GATTTATTTGTTAAAAATAATAAATTATATCAACTACCAATAAAAGAATTGGATGAACTAAGAGTAAATGAAATTAAAGG
TTTTGAAAATGTTTATGCATATGAAAATGGTTTAACTGAAATAAGTGCAACCGATATTAATAATGAATTTGAAATATTAA
TAAAAAATGAAAAAAACGAAAATATAAAAATATCTAATATTAACAATAAATTTATAATAGATAGATCTGAAACAACTTTC
AAAGATGATGAAAATTTACCTCCTTTAATTAACTTAGATGATTTAAATGTTCAAAGTATCAGAATTTTAGTAGATAGAAG
TGTTATGGAAATCTTTATTAATGAAGGTGAACATGCTGTTTCAGTAAGATTCTATATAAATGAACATAACAAAATTCTAA
CAAATTTAAAAAATGTAAAAATTAATCAATTAAAAGGGTATTCATATAATTGAAATAATATAATATTCAAGAATGAGATA
AAAGGACAATAA

Upstream 100 bases:

>100_bases
GAAGCTAAAAAAACCAAATTAGGTTTAAAATTTGATGAGTTAAAAGCAAAAGTAAAATTTGACAAAATCAAATTTAGAAA
AAACAAAGCGGAGTAATGAC

Downstream 100 bases:

>100_bases
TATGAAAAATATAGTTTCAATTGGAGAAGTGTTAATGGATGTTTATTCAGAAAATAACACAATTAAAAGTGAAGTTGGTG
GAGCATCATTTAATGTTGCT

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MQKEKYSLISNNDLEDIQKWHDKKQSDWYNNQFHLAGYSGSTNDPNGLTYHKGKYYIFMQSCPFSIQHFNKSWALYTTTD
FINYNYEGLTLIPSTKYDINGVFSGSARINENDEIEIYYTGNIKFNDVDRTAYTLKAFIDLKEKLVTKELLFEADLTKYT
GHYRDPIVFEKNNQLYMLNGAQTKDLKAMLNVYRFNGSNWENFKDIKFDEADEQNAYMLECPNYFKLDGREYVFACLEQD
APLKDGSHYVKYREVEIDNDINFTYKTSLLKIDLGFDFYAPQVFSNTGERVIMLGWLGNSKSNPFPPELTTWSNQLTVPR
DLFVKNNKLYQLPIKELDELRVNEIKGFENVYAYENGLTEISATDINNEFEILIKNEKNENIKISNINNKFIIDRSETTF
KDDENLPPLINLDDLNVQSIRILVDRSVMEIFINEGEHAVSVRFYINEHNKILTNLKNVKINQLKGYSYNWNNIIFKNEI
KGQ

Sequences:

>Translated_483_residues
MQKEKYSLISNNDLEDIQK*HDKKQSD*YNNQFHLAGYSGSTNDPNGLTYHKGKYYIFMQSCPFSIQHFNKS*ALYTTTD
FINYNYEGLTLIPSTKYDINGVFSGSARINENDEIEIYYTGNIKFNDVDRTAYTLKAFIDLKEKLVTKELLFEADLTKYT
GHYRDPIVFEKNNQLYMLNGAQTKDLKAMLNVYRFNGSN*ENFKDIKFDEADEQNAYMLECPNYFKLDGREYVFACLEQD
APLKDGSHYVKYREVEIDNDINFTYKTSLLKIDLGFDFYAPQVFSNTGERVIMLG*LGNSKSNPFPPELTT*SNQLTVPR
DLFVKNNKLYQLPIKELDELRVNEIKGFENVYAYENGLTEISATDINNEFEILIKNEKNENIKISNINNKFIIDRSETTF
KDDENLPPLINLDDLNVQSIRILVDRSVMEIFINEGEHAVSVRFYINEHNKILTNLKNVKINQLKGYSYN*NNIIFKNEI
KGQ
>Mature_483_residues
MQKEKYSLISNNDLEDIQK*HDKKQSD*YNNQFHLAGYSGSTNDPNGLTYHKGKYYIFMQSCPFSIQHFNKS*ALYTTTD
FINYNYEGLTLIPSTKYDINGVFSGSARINENDEIEIYYTGNIKFNDVDRTAYTLKAFIDLKEKLVTKELLFEADLTKYT
GHYRDPIVFEKNNQLYMLNGAQTKDLKAMLNVYRFNGSN*ENFKDIKFDEADEQNAYMLECPNYFKLDGREYVFACLEQD
APLKDGSHYVKYREVEIDNDINFTYKTSLLKIDLGFDFYAPQVFSNTGERVIMLG*LGNSKSNPFPPELTT*SNQLTVPR
DLFVKNNKLYQLPIKELDELRVNEIKGFENVYAYENGLTEISATDINNEFEILIKNEKNENIKISNINNKFIIDRSETTF
KDDENLPPLINLDDLNVQSIRILVDRSVMEIFINEGEHAVSVRFYINEHNKILTNLKNVKINQLKGYSYN*NNIIFKNEI
KGQ

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 55252; Mature: 55252

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKEKYSLISNNDLEDIQKHDKKQSDYNNQFHLAGYSGSTNDPNGLTYHKGKYYIFMQSC
CCCCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEECCEEEEEEECC
PFSIQHFNKSALYTTTDFINYNYEGLTLIPSTKYDINGVFSGSARINENDEIEIYYTGNI
CCEEECCCCCEEEEEEEEEEECCCCEEEECCCCCCCCEEEECCEEECCCCCEEEEEECCE
KFNDVDRTAYTLKAFIDLKEKLVTKELLFEADLTKYTGHYRDPIVFEKNNQLYMLNGAQT
EEECCCCHHEEHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEECCCEEEEEECCCC
KDLKAMLNVYRFNGSNENFKDIKFDEADEQNAYMLECPNYFKLDGREYVFACLEQDAPLK
HHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCEEEEEEECCCCCCC
DGSHYVKYREVEIDNDINFTYKTSLLKIDLGFDFYAPQVFSNTGERVIMLGLGNSKSNPF
CCCCEEEEEEEEECCCCCEEEEEEEEEEEECCCEECCHHHCCCCCEEEEEECCCCCCCCC
PPELTTSNQLTVPRDLFVKNNKLYQLPIKELDELRVNEIKGFENVYAYENGLTEISATDI
CCCCCCCCCEECCHHHEEECCEEEECCHHHHHHHHHHHHCCCHHEEEECCCCCEEEEECC
NNEFEILIKNEKNENIKISNINNKFIIDRSETTFKDDENLPPLINLDDLNVQSIRILVDR
CCEEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEEEEC
SVMEIFINEGEHAVSVRFYINEHNKILTNLKNVKINQLKGYSYNNNIIFKNEIKGQ
CEEEEEECCCCEEEEEEEEEECCCEEEHHHCCEEEEEEECEEECCCEEEECCCCCC
>Mature Secondary Structure
MQKEKYSLISNNDLEDIQKHDKKQSDYNNQFHLAGYSGSTNDPNGLTYHKGKYYIFMQSC
CCCCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEECCEEEEEEECC
PFSIQHFNKSALYTTTDFINYNYEGLTLIPSTKYDINGVFSGSARINENDEIEIYYTGNI
CCEEECCCCCEEEEEEEEEEECCCCEEEECCCCCCCCEEEECCEEECCCCCEEEEEECCE
KFNDVDRTAYTLKAFIDLKEKLVTKELLFEADLTKYTGHYRDPIVFEKNNQLYMLNGAQT
EEECCCCHHEEHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEECCCEEEEEECCCC
KDLKAMLNVYRFNGSNENFKDIKFDEADEQNAYMLECPNYFKLDGREYVFACLEQDAPLK
HHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCEEEEEEECCCCCCC
DGSHYVKYREVEIDNDINFTYKTSLLKIDLGFDFYAPQVFSNTGERVIMLGLGNSKSNPF
CCCCEEEEEEEEECCCCCEEEEEEEEEEEECCCEECCHHHCCCCCEEEEEECCCCCCCCC
PPELTTSNQLTVPRDLFVKNNKLYQLPIKELDELRVNEIKGFENVYAYENGLTEISATDI
CCCCCCCCCEECCHHHEEECCEEEECCHHHHHHHHHHHHCCCHHEEEECCCCCEEEEECC
NNEFEILIKNEKNENIKISNINNKFIIDRSETTFKDDENLPPLINLDDLNVQSIRILVDR
CCEEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEEEEC
SVMEIFINEGEHAVSVRFYINEHNKILTNLKNVKINQLKGYSYNNNIIFKNEIKGQ
CEEEEEECCCCEEEEEEEEEECCCEEEHHHCCEEEEEEECEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2551785 [H]