Click here to switch to the map view.

The map label for this gene is tpiA [H]

Identifier: 50365321

GI number: 50365321

Start: 591600

End: 592427

Strand: Reverse

Name: tpiA [H]

Synonym: Mfl504

Alternate gene names: 50365321

Gene position: 592427-591600 (Counterclockwise)

Preceding gene: 50365322

Following gene: 50365320

Centisome position: 74.69

GC content: 29.83

Gene sequence:

>828_bases
ATGTTGTTATCTTTTTTATTTCATTGATTTTCTTATTGTTATATAATTAAAAATGTACAAAAAAACAAGAGGAGAAATAT
CATGAGACAAAAAGTAATTTTCGGAAATTGAAAAATGAACGGAACTAATGCTGAACTTGTTACTTTCTTAAAAAAAGTTG
ACAAGGCTGCTAAAAAATCAGATGTAGTTGCAGGATTAGGATTACCATTTACATTATTATCAAAAGGAATTGAAAAAGCA
AAAAATGTTAAAGTTGCTGCACAAAATGTTCACTTTGCTGAAAAAGGAGCATTTACTGGAGAAGTTTCAATTTCAATGTT
ACAAGAAGTAGGAGTACAATACGTTATTATTGGTCACTCAGAAAGAAGAGAAATGTTTGCTGAAACTGATGAAACAGTAA
ACAAAAAAGCTACTGCATTATTAGCTGCAGGAATCACACCAATTATTTGTTGTGGTGAAACTTTAGAAACTAAAGAAGCT
AAAAAAACTGTTACATTTGTAAACGCACAAATTAAAAAAGCATATAAAGGAATTAGTGCAGAGGATGCATTAAAAACAAT
TATTGCTTATGAACCAATCTGAGCTATTGGAACAGGTAAAACTGCAACAAGTGAAGATGCTGAAAAAGTGTGTGAAGCTA
TTCGTAAAAATTTAACAAAAATTTATGACGAAAAAACTGCACAACAAATTACTATTCAATATGGTGGAAGTGTTAACCCA
TCAAACATTGCTGAATTAATGTCACAACCAAACATTGATGGAGCACTTGTTGGTGGAGCTTCATTAAAAGCTGATGATTT
TATTGCATTAATCAATTACAAAAAATAA

Upstream 100 bases:

>100_bases
TCCAGTTTATGAAAAATTTTCAATTTGAATTAACGGAAAAAACGAATTAATGACAACCACTCAAGTTAAAAAATAAAAAT
ATTTTTTGATAACAACTTAA

Downstream 100 bases:

>100_bases
TTAATGAATGAAATTAAGCTTTTAGTTTTAGATATGGATGGTACTTCTTATCACAAGATGGGAAATATTATCGAATCTAA
TATTAAGCCATTACAAGATG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLLSFLFHWFSYCYIIKNVQKNKRRNIMRQKVIFGNWKMNGTNAELVTFLKKVDKAAKKSDVVAGLGLPFTLLSKGIEKA
KNVKVAAQNVHFAEKGAFTGEVSISMLQEVGVQYVIIGHSERREMFAETDETVNKKATALLAAGITPIICCGETLETKEA
KKTVTFVNAQIKKAYKGISAEDALKTIIAYEPIWAIGTGKTATSEDAEKVCEAIRKNLTKIYDEKTAQQITIQYGGSVNP
SNIAELMSQPNIDGALVGGASLKADDFIALINYKK

Sequences:

>Translated_275_residues
MLLSFLFH*FSYCYIIKNVQKNKRRNIMRQKVIFGN*KMNGTNAELVTFLKKVDKAAKKSDVVAGLGLPFTLLSKGIEKA
KNVKVAAQNVHFAEKGAFTGEVSISMLQEVGVQYVIIGHSERREMFAETDETVNKKATALLAAGITPIICCGETLETKEA
KKTVTFVNAQIKKAYKGISAEDALKTIIAYEPI*AIGTGKTATSEDAEKVCEAIRKNLTKIYDEKTAQQITIQYGGSVNP
SNIAELMSQPNIDGALVGGASLKADDFIALINYKK
>Mature_275_residues
MLLSFLFH*FSYCYIIKNVQKNKRRNIMRQKVIFGN*KMNGTNAELVTFLKKVDKAAKKSDVVAGLGLPFTLLSKGIEKA
KNVKVAAQNVHFAEKGAFTGEVSISMLQEVGVQYVIIGHSERREMFAETDETVNKKATALLAAGITPIICCGETLETKEA
KKTVTFVNAQIKKAYKGISAEDALKTIIAYEPI*AIGTGKTATSEDAEKVCEAIRKNLTKIYDEKTAQQITIQYGGSVNP
SNIAELMSQPNIDGALVGGASLKADDFIALINYKK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=280, Percent_Identity=37.1428571428571, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=39.9193548387097, Blast_Score=159, Evalue=2e-39,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=44.578313253012, Blast_Score=187, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17536593, Length=255, Percent_Identity=45.0980392156863, Blast_Score=170, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=250, Percent_Identity=38, Blast_Score=160, Evalue=3e-40,
Organism=Drosophila melanogaster, GI28572004, Length=252, Percent_Identity=39.6825396825397, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=39.7590361445783, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=39.7590361445783, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 29638; Mature: 29638

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSFLFHFSYCYIIKNVQKNKRRNIMRQKVIFGNKMNGTNAELVTFLKKVDKAAKKSDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
VAGLGLPFTLLSKGIEKAKNVKVAAQNVHFAEKGAFTGEVSISMLQEVGVQYVIIGHSER
EECCCCCHHHHHHHHHHHHCCEEEHHCCCHHHCCCCCCHHHHHHHHHCCCEEEEECCCHH
REMFAETDETVNKKATALLAAGITPIICCGETLETKEAKKTVTFVNAQIKKAYKGISAED
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ALKTIIAYEPIAIGTGKTATSEDAEKVCEAIRKNLTKIYDEKTAQQITIQYGGSVNPSNI
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHH
AELMSQPNIDGALVGGASLKADDFIALINYKK
HHHHHCCCCCEEEECCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MLLSFLFHFSYCYIIKNVQKNKRRNIMRQKVIFGNKMNGTNAELVTFLKKVDKAAKKSDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
VAGLGLPFTLLSKGIEKAKNVKVAAQNVHFAEKGAFTGEVSISMLQEVGVQYVIIGHSER
EECCCCCHHHHHHHHHHHHCCEEEHHCCCHHHCCCCCCHHHHHHHHHCCCEEEEECCCHH
REMFAETDETVNKKATALLAAGITPIICCGETLETKEAKKTVTFVNAQIKKAYKGISAED
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ALKTIIAYEPIAIGTGKTATSEDAEKVCEAIRKNLTKIYDEKTAQQITIQYGGSVNPSNI
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHH
AELMSQPNIDGALVGGASLKADDFIALINYKK
HHHHHCCCCCEEEECCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA