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The map label for this gene is proS [H]

Identifier: 50365293

GI number: 50365293

Start: 560510

End: 561931

Strand: Reverse

Name: proS [H]

Synonym: Mfl476

Alternate gene names: 50365293

Gene position: 561931-560510 (Counterclockwise)

Preceding gene: 50365294

Following gene: 50365292

Centisome position: 70.84

GC content: 29.32

Gene sequence:

>1422_bases
ATGAAACAATTAGAGAAAATAACACCAAGAGACGTTGATTTTTCACAATGATATACTGATACAGTTATTAATGCTAAATT
AGCAAGCTATGGTCCAGTTAAAGGAACAATTATCTTTAGACCTTATGGATATGCAATTTGAGAATTAATCCAAAAATATT
TGGATGCAAAATTCAAAGAACTTGAAGTACAAAATGTTTACTTCCCATTATTAATTCCACAATCATTATTTCAAAAAGAA
AAAGACCACATTGAAGGTTTTTCACCTGAGATAGCAACTGTAACAAGAGTTGGAGATACTCCATTACCTGAGCCACTATT
TATTAGACCAACTAGTGAAGTGTTGATGGCAAACTTTTTCAAAAATGAAGTTAAATCATATCGTGACTTACCTTTAATCT
TTAACCAATGAACTAATGTTATGCGTTGAGAAAAAACAACTAGACCATTTTTAAGAACAAGTGAATTCTTATGACAAGAA
GGACACACTGTACATTCAGAAAGAAAAGAAGCTTCTGATTTAACTTTAAAAATATTAGACACATATACTGAGTTTGCTCA
AAATGCATTATTGTTACCTGTAATCCCAGGTAAGAAAACTGAAAAGGAAAAATTTGCGGGAGCTGATTCAACATATACTA
TTGAATCATTAATGCATGATGGTCAAGCATTACAATGTGGTACATCACATTACTTTGCAGATAACTTCTCTAAACCATAT
GAAATTAAATTCCAAAATAAAGAAGGTAAATTAGAGCATGCTTATTCAACAAGTTGAGGAGTTTCAACAAGATTAATTGG
TGCTATTATTATGACTCACTCTGATGATAATGGTTTAGTACTTCCATCAATGGTTTCACCAGTTCAAGTTAGATTAATTC
AAATAAAAGAAACTGATGAAGTTATTAAAGTAACAGAAGATATTAAAGAAGCATTAAAAAATAAATATCGTGTTGACATT
GATAAAACAGATAAATCATTTGGATTTAAAATAAGTGAAGCTGAAATCAAAGGGATTCCTTTAAGAATTGAAATAGGACC
AAGAGATTTAGAAAATAATCAAGTAACCATTTCAAGAAGAGATACTAGAGAAAAAATTCAAGTTAATGTAAATGAAGTTA
CAAATATAGTAGATCAAATGATCAAAGAATATGATGCTAATTTATATGCTAAGGCTTTAAAAAACCGTGAAGAAAGAACT
TCAAGGGCTAACTCAATTGAAGAATACAAAAACATACTAGCTCAAAACCAAGGTTTTGTATTAGTTCCATTCTGTGGTGA
AATTGAATGTGAAGATGATGTTAAAAAACAAACTTCTACAAATTCACGTTGTATTCCATTTGATCAAGATAACAAAACTG
AAAAATGTTTTAACTGTAACAAAGACACAACATTAAAAGTTTATTTTGCAAGAGCTTATTAA

Upstream 100 bases:

>100_bases
CAATACTATAACTATGCTAATAACATTATCAAGGAATTAGGATTAGAAATAAAATTATATTTAAGTCATGATGGCTTAGT
AAAAATAGGAGAGTAATAAA

Downstream 100 bases:

>100_bases
TTAATAAAAGTAAGGAGGTTTAGAAATGAAATATTACGCTGTTAAACAAGGAAGAAATATTGGTGTATATGAAACTTGAG
AAGAATGTAAAATTCAAGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKQLEKITPRDVDFSQWYTDTVINAKLASYGPVKGTIIFRPYGYAIWELIQKYLDAKFKELEVQNVYFPLLIPQSLFQKE
KDHIEGFSPEIATVTRVGDTPLPEPLFIRPTSEVLMANFFKNEVKSYRDLPLIFNQWTNVMRWEKTTRPFLRTSEFLWQE
GHTVHSERKEASDLTLKILDTYTEFAQNALLLPVIPGKKTEKEKFAGADSTYTIESLMHDGQALQCGTSHYFADNFSKPY
EIKFQNKEGKLEHAYSTSWGVSTRLIGAIIMTHSDDNGLVLPSMVSPVQVRLIQIKETDEVIKVTEDIKEALKNKYRVDI
DKTDKSFGFKISEAEIKGIPLRIEIGPRDLENNQVTISRRDTREKIQVNVNEVTNIVDQMIKEYDANLYAKALKNREERT
SRANSIEEYKNILAQNQGFVLVPFCGEIECEDDVKKQTSTNSRCIPFDQDNKTEKCFNCNKDTTLKVYFARAY

Sequences:

>Translated_473_residues
MKQLEKITPRDVDFSQ*YTDTVINAKLASYGPVKGTIIFRPYGYAI*ELIQKYLDAKFKELEVQNVYFPLLIPQSLFQKE
KDHIEGFSPEIATVTRVGDTPLPEPLFIRPTSEVLMANFFKNEVKSYRDLPLIFNQ*TNVMR*EKTTRPFLRTSEFL*QE
GHTVHSERKEASDLTLKILDTYTEFAQNALLLPVIPGKKTEKEKFAGADSTYTIESLMHDGQALQCGTSHYFADNFSKPY
EIKFQNKEGKLEHAYSTS*GVSTRLIGAIIMTHSDDNGLVLPSMVSPVQVRLIQIKETDEVIKVTEDIKEALKNKYRVDI
DKTDKSFGFKISEAEIKGIPLRIEIGPRDLENNQVTISRRDTREKIQVNVNEVTNIVDQMIKEYDANLYAKALKNREERT
SRANSIEEYKNILAQNQGFVLVPFCGEIECEDDVKKQTSTNSRCIPFDQDNKTEKCFNCNKDTTLKVYFARAY
>Mature_473_residues
MKQLEKITPRDVDFSQ*YTDTVINAKLASYGPVKGTIIFRPYGYAI*ELIQKYLDAKFKELEVQNVYFPLLIPQSLFQKE
KDHIEGFSPEIATVTRVGDTPLPEPLFIRPTSEVLMANFFKNEVKSYRDLPLIFNQ*TNVMR*EKTTRPFLRTSEFL*QE
GHTVHSERKEASDLTLKILDTYTEFAQNALLLPVIPGKKTEKEKFAGADSTYTIESLMHDGQALQCGTSHYFADNFSKPY
EIKFQNKEGKLEHAYSTS*GVSTRLIGAIIMTHSDDNGLVLPSMVSPVQVRLIQIKETDEVIKVTEDIKEALKNKYRVDI
DKTDKSFGFKISEAEIKGIPLRIEIGPRDLENNQVTISRRDTREKIQVNVNEVTNIVDQMIKEYDANLYAKALKNREERT
SRANSIEEYKNILAQNQGFVLVPFCGEIECEDDVKKQTSTNSRCIPFDQDNKTEKCFNCNKDTTLKVYFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=491, Percent_Identity=40.3258655804481, Blast_Score=333, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI71991342, Length=493, Percent_Identity=37.525354969574, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI71991346, Length=364, Percent_Identity=40.9340659340659, Blast_Score=271, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6321808, Length=502, Percent_Identity=38.8446215139442, Blast_Score=326, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24649468, Length=488, Percent_Identity=36.8852459016393, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24649466, Length=488, Percent_Identity=36.8852459016393, Blast_Score=309, Evalue=2e-84,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 53410; Mature: 53410

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLEKITPRDVDFSQYTDTVINAKLASYGPVKGTIIFRPYGYAIELIQKYLDAKFKELE
CCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHEE
VQNVYFPLLIPQSLFQKEKDHIEGFSPEIATVTRVGDTPLPEPLFIRPTSEVLMANFFKN
CCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHHH
EVKSYRDLPLIFNQTNVMREKTTRPFLRTSEFLQEGHTVHSERKEASDLTLKILDTYTEF
HHHHHCCCCEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCEEHHHHHHHHH
AQNALLLPVIPGKKTEKEKFAGADSTYTIESLMHDGQALQCGTSHYFADNFSKPYEIKFQ
HCCCEEEEECCCCCCCHHHHCCCCCCEEHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEC
NKEGKLEHAYSTSGVSTRLIGAIIMTHSDDNGLVLPSMVSPVQVRLIQIKETDEVIKVTE
CCCCCEEEEECCCCCHHHEEEEEEEEECCCCCEECCCCCCCEEEEEEEEECCCHHHHHHH
DIKEALKNKYRVDIDKTDKSFGFKISEAEIKGIPLRIEIGPRDLENNQVTISRRDTREKI
HHHHHHHCCEEEEECCCCCCCCEEEECCEECCCEEEEEECCCCCCCCEEEEEECCCCCCE
QVNVNEVTNIVDQMIKEYDANLYAKALKNREERTSRANSIEEYKNILAQNQGFVLVPFCG
EEEHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
EIECEDDVKKQTSTNSRCIPFDQDNKTEKCFNCNKDTTLKVYFARAY
CCCCCHHHHHHCCCCCEECCCCCCCCCHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKQLEKITPRDVDFSQYTDTVINAKLASYGPVKGTIIFRPYGYAIELIQKYLDAKFKELE
CCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHEE
VQNVYFPLLIPQSLFQKEKDHIEGFSPEIATVTRVGDTPLPEPLFIRPTSEVLMANFFKN
CCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHHH
EVKSYRDLPLIFNQTNVMREKTTRPFLRTSEFLQEGHTVHSERKEASDLTLKILDTYTEF
HHHHHCCCCEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCEEHHHHHHHHH
AQNALLLPVIPGKKTEKEKFAGADSTYTIESLMHDGQALQCGTSHYFADNFSKPYEIKFQ
HCCCEEEEECCCCCCCHHHHCCCCCCEEHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEC
NKEGKLEHAYSTSGVSTRLIGAIIMTHSDDNGLVLPSMVSPVQVRLIQIKETDEVIKVTE
CCCCCEEEEECCCCCHHHEEEEEEEEECCCCCEECCCCCCCEEEEEEEEECCCHHHHHHH
DIKEALKNKYRVDIDKTDKSFGFKISEAEIKGIPLRIEIGPRDLENNQVTISRRDTREKI
HHHHHHHCCEEEEECCCCCCCCEEEECCEECCCEEEEEECCCCCCCCEEEEEECCCCCCE
QVNVNEVTNIVDQMIKEYDANLYAKALKNREERTSRANSIEEYKNILAQNQGFVLVPFCG
EEEHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
EIECEDDVKKQTSTNSRCIPFDQDNKTEKCFNCNKDTTLKVYFARAY
CCCCCHHHHHHCCCCCEECCCCCCCCCHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA