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The map label for this gene is gmuD [H]

Identifier: 50365243

GI number: 50365243

Start: 498236

End: 499594

Strand: Direct

Name: gmuD [H]

Synonym: Mfl425

Alternate gene names: 50365243

Gene position: 498236-499594 (Clockwise)

Preceding gene: 50365199

Following gene: 50365244

Centisome position: 62.81

GC content: 28.84

Gene sequence:

>1359_bases
ATGTTAAAATTTCCTAAAAATTTTCACATCGGTGCTTCAATGAGTGCTATGCAAACAGAAGGAAAAGGAATTACTGAAAT
AGGTGATTTAACTTTTGATGCATATTTCAAAGAAAATCCGGAATTGTTTTACCATGGTGTTGGGCCAGATCTGACAAGTG
ATATTACAAGACACTATAAAGATGATATTGAAAAATTTAAATACATCGGATTAGATTCAGTTAGAACAGGTTTTTCTTGA
GCTAGATTATTTCCAGATGGTATTAATCTAAACAAAGAAGCAGTAAAGTTCTATCATGACTATATCGATGAGTATTTAAA
AAATGATATTGAAATTATTATGACTTTATTTCACTTTGACATGCCTTTATGAGCACATGAATTAGGTGGTTGAGAGAGCA
GAGAAGTTATTGAAAAATTTATAAGTTATTGTGAATTTGTATTCAAGGAATATGGATCAAAAATAAATTATTTTGTTACC
TTCAATGAACCACTTGTTCCTGTATTTGAAGGATATGTAGGTAAAATGCACTATCCCGCAAAGGATAGTCCCAAAGAAGC
TGTAGCTCAAGCATATGGAATTTTCCTAGCTCATGCTAAAGCAGTAAAGTTATTTAAAGAATTAAAAATTGATTCAAAAA
TAGGAGTTGTTTATAACTGAAACTTTACATTCCCATTTTCAGATTCAGCAGAAGATAAAATTTCAGCTGAAATCTATGAT
GCTTATGTAAATAGAGGACCATTAAACATTATGTATAATGGAAATATTAACCCAATTATTATAAAAACCTTAGAAGAATA
TAACATAACTCCATTTCACACAAGCGAAGAAATTGAAATAATTAAACAAACTGAAATTGATTTTTTAGGAGTTAATTATT
ATTTCCCTTGTAGAGTTAAAACAAATGAAAATGTAAAAAATAGATGAGCTTTAGATCAAATGCATATTGAAATTCCTGCA
GATGCAAAAATTAATCCTTTTAGAGGGTGAGAAATTTATCCTGAAGGGCTATATGATATATCTATAGCAATTAAAAAAGA
GTTAAATAACATTCCATGATACATTGCTGAAAATGGTATGGGTGTTGAAAATGAAGATAGATTTAGAAATGAAAATGGAC
AAATAGATGATGATTACAGAATTGAGTTTTTAGAAACTCATATGTCTGAATTAAAAAGAGGTTTAGATGCTGGATCAAAT
TGTTTTGGTTACCACATTTGAGCTGCCATTGACTGCTGAAGCTTTAGAAATGCTTATAAAAATAGATATGGTTTAATTGA
AGTTGATTTAAAAGACCAATCTAGAAAGTTTAAAAAATCAGCTTACTGATATAAAGAACTAATAGAAAATAAGGAGTAA

Upstream 100 bases:

>100_bases
CTTCTCATAAATTTAAGTACTTCTTTATAAATATACTACATGTAAAATTAATAGCAAAATTTTTATATAAAATAAGTGTA
GAAAAAAGAGGATTATAAAA

Downstream 100 bases:

>100_bases
ACTATGGCTACAAAAAGTCAAATATACAAAAATGTTGAAAAATTATTTTTAAGTCTTGGTGGACATGAAAATATTCAATA
TTTCACTCACTGTATGACTA

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLKFPKNFHIGASMSAMQTEGKGITEIGDLTFDAYFKENPELFYHGVGPDLTSDITRHYKDDIEKFKYIGLDSVRTGFSW
ARLFPDGINLNKEAVKFYHDYIDEYLKNDIEIIMTLFHFDMPLWAHELGGWESREVIEKFISYCEFVFKEYGSKINYFVT
FNEPLVPVFEGYVGKMHYPAKDSPKEAVAQAYGIFLAHAKAVKLFKELKIDSKIGVVYNWNFTFPFSDSAEDKISAEIYD
AYVNRGPLNIMYNGNINPIIIKTLEEYNITPFHTSEEIEIIKQTEIDFLGVNYYFPCRVKTNENVKNRWALDQMHIEIPA
DAKINPFRGWEIYPEGLYDISIAIKKELNNIPWYIAENGMGVENEDRFRNENGQIDDDYRIEFLETHMSELKRGLDAGSN
CFGYHIWAAIDCWSFRNAYKNRYGLIEVDLKDQSRKFKKSAYWYKELIENKE

Sequences:

>Translated_452_residues
MLKFPKNFHIGASMSAMQTEGKGITEIGDLTFDAYFKENPELFYHGVGPDLTSDITRHYKDDIEKFKYIGLDSVRTGFS*
ARLFPDGINLNKEAVKFYHDYIDEYLKNDIEIIMTLFHFDMPL*AHELGG*ESREVIEKFISYCEFVFKEYGSKINYFVT
FNEPLVPVFEGYVGKMHYPAKDSPKEAVAQAYGIFLAHAKAVKLFKELKIDSKIGVVYN*NFTFPFSDSAEDKISAEIYD
AYVNRGPLNIMYNGNINPIIIKTLEEYNITPFHTSEEIEIIKQTEIDFLGVNYYFPCRVKTNENVKNR*ALDQMHIEIPA
DAKINPFRG*EIYPEGLYDISIAIKKELNNIP*YIAENGMGVENEDRFRNENGQIDDDYRIEFLETHMSELKRGLDAGSN
CFGYHI*AAIDC*SFRNAYKNRYGLIEVDLKDQSRKFKKSAY*YKELIENKE
>Mature_452_residues
MLKFPKNFHIGASMSAMQTEGKGITEIGDLTFDAYFKENPELFYHGVGPDLTSDITRHYKDDIEKFKYIGLDSVRTGFS*
ARLFPDGINLNKEAVKFYHDYIDEYLKNDIEIIMTLFHFDMPL*AHELGG*ESREVIEKFISYCEFVFKEYGSKINYFVT
FNEPLVPVFEGYVGKMHYPAKDSPKEAVAQAYGIFLAHAKAVKLFKELKIDSKIGVVYN*NFTFPFSDSAEDKISAEIYD
AYVNRGPLNIMYNGNINPIIIKTLEEYNITPFHTSEEIEIIKQTEIDFLGVNYYFPCRVKTNENVKNR*ALDQMHIEIPA
DAKINPFRG*EIYPEGLYDISIAIKKELNNIP*YIAENGMGVENEDRFRNENGQIDDDYRIEFLETHMSELKRGLDAGSN
CFGYHI*AAIDC*SFRNAYKNRYGLIEVDLKDQSRKFKKSAY*YKELIENKE

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=473, Percent_Identity=25.1585623678647, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI110681710, Length=470, Percent_Identity=24.6808510638298, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI32481206, Length=455, Percent_Identity=23.956043956044, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI28376633, Length=486, Percent_Identity=23.2510288065844, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI24497614, Length=222, Percent_Identity=26.5765765765766, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367174, Length=486, Percent_Identity=29.4238683127572, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI2367270, Length=466, Percent_Identity=27.6824034334764, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1789070, Length=484, Percent_Identity=26.4462809917355, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17552856, Length=479, Percent_Identity=30.6889352818372, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17539390, Length=475, Percent_Identity=29.2631578947368, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21356577, Length=464, Percent_Identity=24.1379310344828, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50910; Mature: 50910

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFPKNFHIGASMSAMQTEGKGITEIGDLTFDAYFKENPELFYHGVGPDLTSDITRHYK
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHH
DDIEKFKYIGLDSVRTGFSARLFPDGINLNKEAVKFYHDYIDEYLKNDIEIIMTLFHFDM
HHHHHHHHCCCHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLAHELGGESREVIEKFISYCEFVFKEYGSKINYFVTFNEPLVPVFEGYVGKMHYPAKDS
CHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHCCCCCCCCCCCCC
PKEAVAQAYGIFLAHAKAVKLFKELKIDSKIGVVYNNFTFPFSDSAEDKISAEIYDAYVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEECCCCCCCCHHHHHHHHHHHHC
RGPLNIMYNGNINPIIIKTLEEYNITPFHTSEEIEIIKQTEIDFLGVNYYFPCRVKTNEN
CCCEEEEECCCCCEEEEEEHHHCCCCCCCCCCHHHEEEECCCCEEEECEEEEEEEECCCC
VKNRALDQMHIEIPADAKINPFRGEIYPEGLYDISIAIKKELNNIPYIAENGMGVENEDR
HHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCEEECCCCCCCCHHH
FRNENGQIDDDYRIEFLETHMSELKRGLDAGSNCFGYHIAAIDCSFRNAYKNRYGLIEVD
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHCCCCEEEEE
LKDQSRKFKKSAYYKELIENKE
CCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKFPKNFHIGASMSAMQTEGKGITEIGDLTFDAYFKENPELFYHGVGPDLTSDITRHYK
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHH
DDIEKFKYIGLDSVRTGFSARLFPDGINLNKEAVKFYHDYIDEYLKNDIEIIMTLFHFDM
HHHHHHHHCCCHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLAHELGGESREVIEKFISYCEFVFKEYGSKINYFVTFNEPLVPVFEGYVGKMHYPAKDS
CHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHCCCCCCCCCCCCC
PKEAVAQAYGIFLAHAKAVKLFKELKIDSKIGVVYNNFTFPFSDSAEDKISAEIYDAYVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEECCCCCCCCHHHHHHHHHHHHC
RGPLNIMYNGNINPIIIKTLEEYNITPFHTSEEIEIIKQTEIDFLGVNYYFPCRVKTNEN
CCCEEEEECCCCCEEEEEEHHHCCCCCCCCCCHHHEEEECCCCEEEECEEEEEEEECCCC
VKNRALDQMHIEIPADAKINPFRGEIYPEGLYDISIAIKKELNNIPYIAENGMGVENEDR
HHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCEEECCCCCCCCHHH
FRNENGQIDDDYRIEFLETHMSELKRGLDAGSNCFGYHIAAIDCSFRNAYKNRYGLIEVD
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHCCCCEEEEE
LKDQSRKFKKSAYYKELIENKE
CCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]