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The map label for this gene is treP [H]

Identifier: 50365244

GI number: 50365244

Start: 499598

End: 501229

Strand: Direct

Name: treP [H]

Synonym: Mfl426

Alternate gene names: 50365244

Gene position: 499598-501229 (Clockwise)

Preceding gene: 50365243

Following gene: 50365252

Centisome position: 62.98

GC content: 30.02

Gene sequence:

>1632_bases
ATGGCTACAAAAAGTCAAATATACAAAAATGTTGAAAAATTATTTTTAAGTCTTGGTGGACATGAAAATATTCAATATTT
CACTCACTGTATGACTAGAATGCGTTTTCATTTGCATGATTGAGATAAAGCAAATGAAAATGAAATTAAAGATGGTGGTT
ATGCTGTTGGTGTTAACAAAAATAAAAGCAACGGTGAATTCCAAGTTATTATAGGTCCTGAAGTTGAAAGTTTTTACAAT
GCTTTTTGCGAAGTTAATGGATATGATGAAGATGGAAAAACATTAATTGTTAAAAATGATAAACCAAATTTAACAGAGAA
ACAAAATAAAGAAATTGTTGATATGAAAAATAGATTTAGAGTTAAAGGTTCATTTAACAAAGCCCTTTCTTTTATCTCAA
AAGTTTTTGCCCCAATAGTTATACCTCTTGTAGGTTATGGATTGATTTTGACAATCGCATCATTGATAACTGTTGAATGA
AGTGGATCAGATAGTAGTTTAGCTGCTAATTCACATTTTTTTAAAGAGTTTTCTGGAATACTTTCAATTCTTGTTAATTC
ATTTTCATTGTTTGTTACAGTTGCTGTAGGTTATACAACAGCTAAAGCATTAAGATGTAATGGAATTTATGGAATTATAA
TTGCACTTGTTTTAACATCACCAGGATTAATAAATATGGGAGATGTTAAACCAGCTGATGGTCAAAGTATTTTAGGAGCT
TATGACGGATGAACATTATTTGGAGATGGAGTTAAATATCCATGAAAAATAAACTTCAATGGATTAATTATTCCAATGAT
AGTTGTTGTTTCATTTGGTGCATTCTTAGAAATACAAACTAATAAAATTAAACAAAGTACATTAAAAATGATTATTCAAC
CAATTGCTATTATTGGTGGAGGTTTCTTATTTGGAGTATTCATAGTAGCACCTGTTGGGTTACTATTCACAAACTACTTA
TCAATTGGTATAAATTGATTAAGTACAAATAGTATTGCTAAATATATTGCTATACCTGTTGTTGGAGGATTATATGGCCC
TTTAGTTATAACTGGTTTACACCACTCATTAACTCCAATTATTTTACAAGGTCAAGCAATTTATGGAGCCACATTGATTC
AAGGTTTCTGTACTATTTCAAACGTTTCACAAGGGGTTGCTTCAATTGCATTCGTTGTGTTACACAGAAGAGTTTCAAAA
ATGAAAGATATTGGAGTTTCAAACGGGGTTTCTGCCATTGTTGGAGGAATCACAGAACCATCATTATATACAATAAACTT
AAAACATTTATTCCCACTAATCGGATGTTCAATAGGTACATTCTTTGGAACAATGATTATGGTTGCTTCTAATTCATATG
CATTGCAAGGGGCTTCTTCAATTTTCGGAATACTTATGTTCTTACAACAAGCACCTGAAAAAACTGGAGCTACAACTTGA
ATTGGTGGAGGATACTTATGAGGTACAATCTCAGTATTAACTTCATGTATAATAACTTTTGTTGCAACTTATTCTTTAGG
TAAAACTAAATACTTCTGAAATAGATCAAGAGAAATCTTATTAAATGATTTCCATGAAGATATTAATGAATTAAAACTTT
TACCAAAAACTAAAAAAGCGAAAAGAGCTTAA

Upstream 100 bases:

>100_bases
TAGATATGGTTTAATTGAAGTTGATTTAAAAGACCAATCTAGAAAGTTTAAAAAATCAGCTTACTGATATAAAGAACTAA
TAGAAAATAAGGAGTAAACT

Downstream 100 bases:

>100_bases
TATAAAAAATTCACATTATTTGTGAATTTTTTATATTATAAATATTCTGCTTTCATTTCTCCAAGTATACTTGATAATCC
TTTTTGTACTTTATTAAAGA

Product: trehalose/sucrose/beta-glucoside PTS system IIBC component

Products: NA

Alternate protein names: EIIBC-Tre; EII-Tre; Trehalose-specific phosphotransferase enzyme IIB component; PTS system trehalose-specific EIIB component; Trehalose permease IIC component; PTS system trehalose-specific EIIC component [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MATKSQIYKNVEKLFLSLGGHENIQYFTHCMTRMRFHLHDWDKANENEIKDGGYAVGVNKNKSNGEFQVIIGPEVESFYN
AFCEVNGYDEDGKTLIVKNDKPNLTEKQNKEIVDMKNRFRVKGSFNKALSFISKVFAPIVIPLVGYGLILTIASLITVEW
SGSDSSLAANSHFFKEFSGILSILVNSFSLFVTVAVGYTTAKALRCNGIYGIIIALVLTSPGLINMGDVKPADGQSILGA
YDGWTLFGDGVKYPWKINFNGLIIPMIVVVSFGAFLEIQTNKIKQSTLKMIIQPIAIIGGGFLFGVFIVAPVGLLFTNYL
SIGINWLSTNSIAKYIAIPVVGGLYGPLVITGLHHSLTPIILQGQAIYGATLIQGFCTISNVSQGVASIAFVVLHRRVSK
MKDIGVSNGVSAIVGGITEPSLYTINLKHLFPLIGCSIGTFFGTMIMVASNSYALQGASSIFGILMFLQQAPEKTGATTW
IGGGYLWGTISVLTSCIITFVATYSLGKTKYFWNRSREILLNDFHEDINELKLLPKTKKAKRA

Sequences:

>Translated_543_residues
MATKSQIYKNVEKLFLSLGGHENIQYFTHCMTRMRFHLHD*DKANENEIKDGGYAVGVNKNKSNGEFQVIIGPEVESFYN
AFCEVNGYDEDGKTLIVKNDKPNLTEKQNKEIVDMKNRFRVKGSFNKALSFISKVFAPIVIPLVGYGLILTIASLITVE*
SGSDSSLAANSHFFKEFSGILSILVNSFSLFVTVAVGYTTAKALRCNGIYGIIIALVLTSPGLINMGDVKPADGQSILGA
YDG*TLFGDGVKYP*KINFNGLIIPMIVVVSFGAFLEIQTNKIKQSTLKMIIQPIAIIGGGFLFGVFIVAPVGLLFTNYL
SIGIN*LSTNSIAKYIAIPVVGGLYGPLVITGLHHSLTPIILQGQAIYGATLIQGFCTISNVSQGVASIAFVVLHRRVSK
MKDIGVSNGVSAIVGGITEPSLYTINLKHLFPLIGCSIGTFFGTMIMVASNSYALQGASSIFGILMFLQQAPEKTGATT*
IGGGYL*GTISVLTSCIITFVATYSLGKTKYF*NRSREILLNDFHEDINELKLLPKTKKAKRA
>Mature_542_residues
ATKSQIYKNVEKLFLSLGGHENIQYFTHCMTRMRFHLHD*DKANENEIKDGGYAVGVNKNKSNGEFQVIIGPEVESFYNA
FCEVNGYDEDGKTLIVKNDKPNLTEKQNKEIVDMKNRFRVKGSFNKALSFISKVFAPIVIPLVGYGLILTIASLITVE*S
GSDSSLAANSHFFKEFSGILSILVNSFSLFVTVAVGYTTAKALRCNGIYGIIIALVLTSPGLINMGDVKPADGQSILGAY
DG*TLFGDGVKYP*KINFNGLIIPMIVVVSFGAFLEIQTNKIKQSTLKMIIQPIAIIGGGFLFGVFIVAPVGLLFTNYLS
IGIN*LSTNSIAKYIAIPVVGGLYGPLVITGLHHSLTPIILQGQAIYGATLIQGFCTISNVSQGVASIAFVVLHRRVSKM
KDIGVSNGVSAIVGGITEPSLYTINLKHLFPLIGCSIGTFFGTMIMVASNSYALQGASSIFGILMFLQQAPEKTGATT*I
GGGYL*GTISVLTSCIITFVATYSLGKTKYF*NRSREILLNDFHEDINELKLLPKTKKAKRA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=429, Percent_Identity=25.6410256410256, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790159, Length=499, Percent_Identity=25.4509018036072, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI48994906, Length=445, Percent_Identity=25.3932584269663, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788769, Length=450, Percent_Identity=23.5555555555556, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011296 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 57647; Mature: 57516

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKSQIYKNVEKLFLSLGGHENIQYFTHCMTRMRFHLHDDKANENEIKDGGYAVGVNKN
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCC
KSNGEFQVIIGPEVESFYNAFCEVNGYDEDGKTLIVKNDKPNLTEKQNKEIVDMKNRFRV
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCHHEEE
KGSFNKALSFISKVFAPIVIPLVGYGLILTIASLITVESGSDSSLAANSHFFKEFSGILS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHH
ILVNSFSLFVTVAVGYTTAKALRCNGIYGIIIALVLTSPGLINMGDVKPADGQSILGAYD
HHHHHHHHHHHEEHHHHHHHHHEECCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCC
GTLFGDGVKYPKINFNGLIIPMIVVVSFGAFLEIQTNKIKQSTLKMIIQPIAIIGGGFLF
CCEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHH
GVFIVAPVGLLFTNYLSIGINLSTNSIAKYIAIPVVGGLYGPLVITGLHHSLTPIILQGQ
HHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEECC
AIYGATLIQGFCTISNVSQGVASIAFVVLHRRVSKMKDIGVSNGVSAIVGGITEPSLYTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEE
NLKHLFPLIGCSIGTFFGTMIMVASNSYALQGASSIFGILMFLQQAPEKTGATTIGGGYL
EHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCEEECCCHH
GTISVLTSCIITFVATYSLGKTKYFNRSREILLNDFHEDINELKLLPKTKKAKRA
HHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
ATKSQIYKNVEKLFLSLGGHENIQYFTHCMTRMRFHLHDDKANENEIKDGGYAVGVNKN
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCC
KSNGEFQVIIGPEVESFYNAFCEVNGYDEDGKTLIVKNDKPNLTEKQNKEIVDMKNRFRV
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCHHEEE
KGSFNKALSFISKVFAPIVIPLVGYGLILTIASLITVESGSDSSLAANSHFFKEFSGILS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHH
ILVNSFSLFVTVAVGYTTAKALRCNGIYGIIIALVLTSPGLINMGDVKPADGQSILGAYD
HHHHHHHHHHHEEHHHHHHHHHEECCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCC
GTLFGDGVKYPKINFNGLIIPMIVVVSFGAFLEIQTNKIKQSTLKMIIQPIAIIGGGFLF
CCEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHH
GVFIVAPVGLLFTNYLSIGINLSTNSIAKYIAIPVVGGLYGPLVITGLHHSLTPIILQGQ
HHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEECC
AIYGATLIQGFCTISNVSQGVASIAFVVLHRRVSKMKDIGVSNGVSAIVGGITEPSLYTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEE
NLKHLFPLIGCSIGTFFGTMIMVASNSYALQGASSIFGILMFLQQAPEKTGATTIGGGYL
EHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCEEECCCHH
GTISVLTSCIITFVATYSLGKTKYFNRSREILLNDFHEDINELKLLPKTKKAKRA
HHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917076; 8969503; 9272861; 9384377; 7651129 [H]