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The map label for this gene is mtnN [H]

Identifier: 50365190

GI number: 50365190

Start: 432735

End: 433385

Strand: Reverse

Name: mtnN [H]

Synonym: Mfl372

Alternate gene names: 50365190

Gene position: 433385-432735 (Counterclockwise)

Preceding gene: 50365191

Following gene: 50365189

Centisome position: 54.64

GC content: 24.12

Gene sequence:

>651_bases
ATGAAATTAATTATAGGAGCTATGCATGAAGAATTGCAGGATTCAATTGCTTTTTACAAATTAAATAAAGTTGAAAATGA
AAAGTTTACAATTTATAAAAATGAAGAAATTATGTTTTGTATAACTGGAATTGGTTTAGTTAATGCAGCTGCTCAATTGT
CATATATTTTAAGTAAATACGATATAGATTCAATAATTAATATTGGAACTAGTGGTGGTTGTGATAAAGAATTGAAACAA
GGTGATATTTTAATTATTGATAAAATTTATAACAGCGTTGCTGATGCAACTGCATTTGGTTATGCTTATGGTCAAGTACC
AAGAATGCCAAAATATTATGAAACAAGTAATAAAGATATTATTAAGACTATCAGTAAAGCAAAAATAAAAAATATTGCTT
CATCAGATATATTTATCCACTCAACTGAACAAGTTAAAAATTTTATTAATAAAATTGAAGATAAAATTAGTGTTTTAGAT
ATGGAATGTTTTGCATATGCTCAAACAGCCTATTTGTTTGAAAAAGAGTTTAGTGTAATAAAAATTATTAGTGATGTAAT
TGGAGAAAAAAACACAAACAATGTTCAATTTAACGATTTTATTAAAATAGCTGGAAAAGAAATTTTGGAAATCTTGAAAA
AAATATTATAA

Upstream 100 bases:

>100_bases
TAATAAACCAATATGAAGTTGCAATTAAAACTCATGGAATAGATTCTATTGGCGATAAACTTTTAAAAACTTATAACTTT
TATGTAAAAGGAGAAAAATA

Downstream 100 bases:

>100_bases
TAATAGTATAGAACTCGAGGTGGATTTATGGCAAATCACAAAATTAAAGGTAGAAAAGAAGCTACAATTTTAAGAGAACT
TACAATTATTATTGAAAGAG

Product: 5'-methylthioadenosine nucleosidase/S-adenosylhomocysteine nucleosidase (purine nucleosidase)

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKLIIGAMHEELQDSIAFYKLNKVENEKFTIYKNEEIMFCITGIGLVNAAAQLSYILSKYDIDSIINIGTSGGCDKELKQ
GDILIIDKIYNSVADATAFGYAYGQVPRMPKYYETSNKDIIKTISKAKIKNIASSDIFIHSTEQVKNFINKIEDKISVLD
MECFAYAQTAYLFEKEFSVIKIISDVIGEKNTNNVQFNDFIKIAGKEILEILKKIL

Sequences:

>Translated_216_residues
MKLIIGAMHEELQDSIAFYKLNKVENEKFTIYKNEEIMFCITGIGLVNAAAQLSYILSKYDIDSIINIGTSGGCDKELKQ
GDILIIDKIYNSVADATAFGYAYGQVPRMPKYYETSNKDIIKTISKAKIKNIASSDIFIHSTEQVKNFINKIEDKISVLD
MECFAYAQTAYLFEKEFSVIKIISDVIGEKNTNNVQFNDFIKIAGKEILEILKKIL
>Mature_216_residues
MKLIIGAMHEELQDSIAFYKLNKVENEKFTIYKNEEIMFCITGIGLVNAAAQLSYILSKYDIDSIINIGTSGGCDKELKQ
GDILIIDKIYNSVADATAFGYAYGQVPRMPKYYETSNKDIIKTISKAKIKNIASSDIFIHSTEQVKNFINKIEDKISVLD
MECFAYAQTAYLFEKEFSVIKIISDVIGEKNTNNVQFNDFIKIAGKEILEILKKIL

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=225, Percent_Identity=32, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 24430; Mature: 24430

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIGAMHEELQDSIAFYKLNKVENEKFTIYKNEEIMFCITGIGLVNAAAQLSYILSKY
CCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHH
DIDSIINIGTSGGCDKELKQGDILIIDKIYNSVADATAFGYAYGQVPRMPKYYETSNKDI
CHHHHHCCCCCCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHH
IKTISKAKIKNIASSDIFIHSTEQVKNFINKIEDKISVLDMECFAYAQTAYLFEKEFSVI
HHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIISDVIGEKNTNNVQFNDFIKIAGKEILEILKKIL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKLIIGAMHEELQDSIAFYKLNKVENEKFTIYKNEEIMFCITGIGLVNAAAQLSYILSKY
CCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHH
DIDSIINIGTSGGCDKELKQGDILIIDKIYNSVADATAFGYAYGQVPRMPKYYETSNKDI
CHHHHHCCCCCCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHH
IKTISKAKIKNIASSDIFIHSTEQVKNFINKIEDKISVLDMECFAYAQTAYLFEKEFSVI
HHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIISDVIGEKNTNNVQFNDFIKIAGKEILEILKKIL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA