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The map label for this gene is hisS [H]

Identifier: 50365183

GI number: 50365183

Start: 425652

End: 427025

Strand: Reverse

Name: hisS [H]

Synonym: Mfl366

Alternate gene names: 50365183

Gene position: 427025-425652 (Counterclockwise)

Preceding gene: 50365184

Following gene: 50365182

Centisome position: 53.83

GC content: 25.33

Gene sequence:

>1374_bases
ATGAAGACGAACAAGAACAAAACTAATATCAAAATGATAGGTTTCTATCATTTTTTTTATTTTGTAAAACCTTATTATTT
GTTAATAAATGAAAATGTTATAATATTTAAAGCAAAAGGAGATTTTATGATTCAAAAACCTAGAGGAACTCAAGATTTAT
TTTTAAATAATTCTAAAGAATGAAATGCAGTAGAAGAAAAATTTAGAAAAGTTCTAAATCTTTTTAACTATGGTGAAATC
ATTACTCCTATGTTTGAATCTAAAGAATTATTTGTTAGAGGTGTTGGAGACTCAAGTGATATTGTAAGCAAAGAAATGTA
TGAGTTTACAGACAGAAAAGGTCGTGAATATGTTTTAAGACCAGAAGGAACAGCTCCAACTGTAAGAGCTTTAATTGAAA
ATAAATTATATATTCAAGAGAATCTACCATACAAAACATTTTATATAGGACCTATTTTTAGATATGAAAGACCACAAGCT
GGAAGATATAGACAATTTAATCAATTAGGGGTAGAAACATTTGGTATAGATTCAATTAGTCATGATGTTGAATTAATTTC
TTTAGGTCAGTCATTCTTAAAAGAATTAAAAATTAATAAAGACGTAATTGTTGAAATGAATTATTTAATAAGTGGAAATG
AAAGAAAAGAATACGAATTAGAACTTAAAAAATATTTGAATTTATTTGATGATTTATGTTCAGATTGTGAAATAAGAATT
AATAAAAATGTTTTACGTGTATTAGATTGTAAAATTGATGGTAATAAATTTGCAGAAGCACCAAAAATGACTTTATTTGC
AAGTCAAGAAAACAAAGAAAGATTGAATAAAACATTTGATCAATTAAAGCAACTAGGAATTGAAGCAAAAATAAACTTTA
ACTTAGTAAGAGGATTAGATTATTACACAGGCTTAGTTTTTGAATTTAAAAATATTAAAACAGACCAAGCTATTATCGCT
GGTGGTTCATACAATAATTTAGTTGAAGAACTAGGTGGACCAAATTTACCTGCTAGTGGTTTTGCAATAGGCATTGAAAG
AATAATGATGATTCTTAGTGATCAAGAAATAAAAGTTGCTGATGAAGATGAATTAGATTTATTTATAATACCGTTGTCTG
ATGATGCTCAATTTTTAACAAATAAACTTTTATTAGAAGCTAGAACAGCAAACCTAAAAGTTGATACAAATTGAAATATT
AAAAATTTAAAAGCTGGATTTAAATCAGCAGAAAGATTGAAATCTAAAAATATCGTTATTATTGGTGAAAATTCAATTAA
TAGTGATATTTATGCAATAAAAGATCAAAAATCTGGTGAAACTAAAGAATTGAAATTTAAAGATATTGTTAAATACTTAA
AAGGGGAAAAATAG

Upstream 100 bases:

>100_bases
AAAGATGTACAAAAAATTATGAAAGCAAGAACTGAACAAGCAAATGCAACAATGGCTGCTATGCAAGGAAAACATTATGA
AATAGATCCTTCATTATTTG

Downstream 100 bases:

>100_bases
AAATGAAAAGAACACATAATTGTAATCAATTAAACATTTCAAATGTAAATCAAGAAGTAACATTAAAGGGATGAATTAAA
AAAATTCGTAAAATGGGACA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKTNKNKTNIKMIGFYHFFYFVKPYYLLINENVIIFKAKGDFMIQKPRGTQDLFLNNSKEWNAVEEKFRKVLNLFNYGEI
ITPMFESKELFVRGVGDSSDIVSKEMYEFTDRKGREYVLRPEGTAPTVRALIENKLYIQENLPYKTFYIGPIFRYERPQA
GRYRQFNQLGVETFGIDSISHDVELISLGQSFLKELKINKDVIVEMNYLISGNERKEYELELKKYLNLFDDLCSDCEIRI
NKNVLRVLDCKIDGNKFAEAPKMTLFASQENKERLNKTFDQLKQLGIEAKINFNLVRGLDYYTGLVFEFKNIKTDQAIIA
GGSYNNLVEELGGPNLPASGFAIGIERIMMILSDQEIKVADEDELDLFIIPLSDDAQFLTNKLLLEARTANLKVDTNWNI
KNLKAGFKSAERLKSKNIVIIGENSINSDIYAIKDQKSGETKELKFKDIVKYLKGEK

Sequences:

>Translated_457_residues
MKTNKNKTNIKMIGFYHFFYFVKPYYLLINENVIIFKAKGDFMIQKPRGTQDLFLNNSKE*NAVEEKFRKVLNLFNYGEI
ITPMFESKELFVRGVGDSSDIVSKEMYEFTDRKGREYVLRPEGTAPTVRALIENKLYIQENLPYKTFYIGPIFRYERPQA
GRYRQFNQLGVETFGIDSISHDVELISLGQSFLKELKINKDVIVEMNYLISGNERKEYELELKKYLNLFDDLCSDCEIRI
NKNVLRVLDCKIDGNKFAEAPKMTLFASQENKERLNKTFDQLKQLGIEAKINFNLVRGLDYYTGLVFEFKNIKTDQAIIA
GGSYNNLVEELGGPNLPASGFAIGIERIMMILSDQEIKVADEDELDLFIIPLSDDAQFLTNKLLLEARTANLKVDTN*NI
KNLKAGFKSAERLKSKNIVIIGENSINSDIYAIKDQKSGETKELKFKDIVKYLKGEK
>Mature_457_residues
MKTNKNKTNIKMIGFYHFFYFVKPYYLLINENVIIFKAKGDFMIQKPRGTQDLFLNNSKE*NAVEEKFRKVLNLFNYGEI
ITPMFESKELFVRGVGDSSDIVSKEMYEFTDRKGREYVLRPEGTAPTVRALIENKLYIQENLPYKTFYIGPIFRYERPQA
GRYRQFNQLGVETFGIDSISHDVELISLGQSFLKELKINKDVIVEMNYLISGNERKEYELELKKYLNLFDDLCSDCEIRI
NKNVLRVLDCKIDGNKFAEAPKMTLFASQENKERLNKTFDQLKQLGIEAKINFNLVRGLDYYTGLVFEFKNIKTDQAIIA
GGSYNNLVEELGGPNLPASGFAIGIERIMMILSDQEIKVADEDELDLFIIPLSDDAQFLTNKLLLEARTANLKVDTN*NI
KNLKAGFKSAERLKSKNIVIIGENSINSDIYAIKDQKSGETKELKFKDIVKYLKGEK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=471, Percent_Identity=26.1146496815287, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1788861, Length=419, Percent_Identity=35.5608591885441, Blast_Score=246, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71993686, Length=491, Percent_Identity=22.6069246435845, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71993693, Length=490, Percent_Identity=22.6530612244898, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 52441; Mature: 52441

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNKNKTNIKMIGFYHFFYFVKPYYLLINENVIIFKAKGDFMIQKPRGTQDLFLNNSKE
CCCCCCCCEEEEEHHHHHHHHHCCEEEEEECCEEEEEECCCEEEECCCCCHHEEECCCCC
NAVEEKFRKVLNLFNYGEIITPMFESKELFVRGVGDSSDIVSKEMYEFTDRKGREYVLRP
HHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECC
EGTAPTVRALIENKLYIQENLPYKTFYIGPIFRYERPQAGRYRQFNQLGVETFGIDSISH
CCCCHHHHHHHCCCEEEECCCCEEEEEECCEEEECCCCCCCCHHHHHCCCEEECCCCCCC
DVELISLGQSFLKELKINKDVIVEMNYLISGNERKEYELELKKYLNLFDDLCSDCEIRIN
CHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
KNVLRVLDCKIDGNKFAEAPKMTLFASQENKERLNKTFDQLKQLGIEAKINFNLVRGLDY
CCEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCHH
YTGLVFEFKNIKTDQAIIAGGSYNNLVEELGGPNLPASGFAIGIERIMMILSDQEIKVAD
HHEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECC
EDELDLFIIPLSDDAQFLTNKLLLEARTANLKVDTNNIKNLKAGFKSAERLKSKNIVIIG
CCCCEEEEEECCCCHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEE
ENSINSDIYAIKDQKSGETKELKFKDIVKYLKGEK
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKTNKNKTNIKMIGFYHFFYFVKPYYLLINENVIIFKAKGDFMIQKPRGTQDLFLNNSKE
CCCCCCCCEEEEEHHHHHHHHHCCEEEEEECCEEEEEECCCEEEECCCCCHHEEECCCCC
NAVEEKFRKVLNLFNYGEIITPMFESKELFVRGVGDSSDIVSKEMYEFTDRKGREYVLRP
HHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECC
EGTAPTVRALIENKLYIQENLPYKTFYIGPIFRYERPQAGRYRQFNQLGVETFGIDSISH
CCCCHHHHHHHCCCEEEECCCCEEEEEECCEEEECCCCCCCCHHHHHCCCEEECCCCCCC
DVELISLGQSFLKELKINKDVIVEMNYLISGNERKEYELELKKYLNLFDDLCSDCEIRIN
CHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
KNVLRVLDCKIDGNKFAEAPKMTLFASQENKERLNKTFDQLKQLGIEAKINFNLVRGLDY
CCEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCHH
YTGLVFEFKNIKTDQAIIAGGSYNNLVEELGGPNLPASGFAIGIERIMMILSDQEIKVAD
HHEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEECC
EDELDLFIIPLSDDAQFLTNKLLLEARTANLKVDTNNIKNLKAGFKSAERLKSKNIVIIG
CCCCEEEEEECCCCHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEE
ENSINSDIYAIKDQKSGETKELKFKDIVKYLKGEK
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA