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The map label for this gene is guaA [H]

Identifier: 50365158

GI number: 50365158

Start: 397160

End: 398701

Strand: Direct

Name: guaA [H]

Synonym: Mfl342

Alternate gene names: 50365158

Gene position: 397160-398701 (Clockwise)

Preceding gene: 50365157

Following gene: 50365159

Centisome position: 50.07

GC content: 28.73

Gene sequence:

>1542_bases
ATGAAAAATACTCAAATCTTGATTTTAGATTTTGGAAGTCAATATACACAGTTATTAGCTAGAAGAGTTAGAGAAGCTAA
TATTTATACAGAAGTTTTACCTTTTGATACATCAATTGAAAAAATTAAAGAATATCCACTTTTAAAAGGTATCATTCTTT
CTGGTGGGCCTTCAAGTGTATATTTACAAAATGCTTATAAAATAGATTCTGAAATCTTAAATTTAGATATTGCTATTTTA
GGTGTATGTTATGGTATGCAATTATTAACTCAATATTTTCAAGGTGCTGTTGAATTGGCTGATGAACAAGAATTTGGTAA
AGCAATCATAACTATTGATGATAAAAAAAATCCACTTTTTAAAAATGTAACTACTGAGTCTCAAGTTTGAATGAGTCATG
CTGATCATGTTACAGAGTTACCAGTAGATTTTAAACAGATTGCACATTCTAACGCAAGTATTGCAGCTATTGCTCATAAT
ACTAAACCAATTTATGGTATTCAATTCCATGCTGAAGTGACTCATTCTTTACAAGGTAAAGAAATGTTAGAAAACTTCTT
ATATGATATTGCTAAATGTAATAAAGACTGAAATCTTGATGACTTTATTGATCAACAAATCAAAGAAATCAAAGAAACTG
TTAAAGATAAGCAAGTTATTTTGGGTTTAAGTGGTGGAGTTGATTCATCAGTTGCTGCTGCTATTATTGGTAAAGCTATT
GGTAAACAACTTACTTGTATCTTTGTTGATACTGGTTTATTAAGAAAAAATGAAACAATTGAAGTTATGAAAGCTTATGA
AACTAATTTTGATATTAATATTAAGTTAGTTGAAGCAAGTGATCTTTTCTTTTCTGAATTAAAAGGAATTAAAGAGCCTG
AAGCTAAAAGAAAAATTATTGGTAAATGTTTTATAGACGTTTTTACAGATGCGGCTAGACAACATAAGGATGCTGATTTC
TTAGCACAAGGAACTATTTATCCAGATGTTATTGAATCTTCTTCTCATGGAGCTAGTTCTAAAACAATTAAATCTCATCA
CAATGTTGGTGGATTACCAGAAGATTTAAAATTTAGATTAATTGAACCTTTAAGAAACTTATTTAAAGATGAAGTAAGAC
AAGTTGGTTTTAAATTGGGATTACCTGAAGAAATGATTAATCGTCATCCTTTCCCTGGACCTGGGCTAGGAATTAGAGTT
ATTGAAGAAGTAACTAAAGATAAAGTTGAAATTTTACAAGAAGCAGATGCTATATTTATTAAAAAATTAAGGGAATGAAA
TTTATATAATACAGTTTCACAAGCCTTTGTTACATTATTACCAGTTAAAACAGTTGGTGTAATGGGTGATAATCGTACTT
ATGATTATGTTGTTGCTTTAAGAAGTGTTAATACAATTGATTTTATGACAGCAACTAGCACACATCTTCCTTGAGAATTT
TTAGATGAAGTAGTAAATGAAATAATAAATAAAGTAGATAATGTTAACCGAGTAGTTTATGATGTTACATCTAAACCTCC
AGGAACAATAGAATGAGAATAA

Upstream 100 bases:

>100_bases
CATATTGGGTTGTCGACCTGCCTCTGGACCTATCCTTAGACTATAAGCGTGAGGTTTTTTTACACAAAAAAGCCTCTTTT
TTATTTAGAAGGAGATTAAA

Downstream 100 bases:

>100_bases
ATATGAATAAAGATTTAAATGGAAAAATTATAAATGAAGCAATTACTTTTGATGATGTACTATTAGTACCTAATTACTCA
GAAGTTTTACCTCATGAAGT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKNTQILILDFGSQYTQLLARRVREANIYTEVLPFDTSIEKIKEYPLLKGIILSGGPSSVYLQNAYKIDSEILNLDIAIL
GVCYGMQLLTQYFQGAVELADEQEFGKAIITIDDKKNPLFKNVTTESQVWMSHADHVTELPVDFKQIAHSNASIAAIAHN
TKPIYGIQFHAEVTHSLQGKEMLENFLYDIAKCNKDWNLDDFIDQQIKEIKETVKDKQVILGLSGGVDSSVAAAIIGKAI
GKQLTCIFVDTGLLRKNETIEVMKAYETNFDINIKLVEASDLFFSELKGIKEPEAKRKIIGKCFIDVFTDAARQHKDADF
LAQGTIYPDVIESSSHGASSKTIKSHHNVGGLPEDLKFRLIEPLRNLFKDEVRQVGFKLGLPEEMINRHPFPGPGLGIRV
IEEVTKDKVEILQEADAIFIKKLREWNLYNTVSQAFVTLLPVKTVGVMGDNRTYDYVVALRSVNTIDFMTATSTHLPWEF
LDEVVNEIINKVDNVNRVVYDVTSKPPGTIEWE

Sequences:

>Translated_513_residues
MKNTQILILDFGSQYTQLLARRVREANIYTEVLPFDTSIEKIKEYPLLKGIILSGGPSSVYLQNAYKIDSEILNLDIAIL
GVCYGMQLLTQYFQGAVELADEQEFGKAIITIDDKKNPLFKNVTTESQV*MSHADHVTELPVDFKQIAHSNASIAAIAHN
TKPIYGIQFHAEVTHSLQGKEMLENFLYDIAKCNKD*NLDDFIDQQIKEIKETVKDKQVILGLSGGVDSSVAAAIIGKAI
GKQLTCIFVDTGLLRKNETIEVMKAYETNFDINIKLVEASDLFFSELKGIKEPEAKRKIIGKCFIDVFTDAARQHKDADF
LAQGTIYPDVIESSSHGASSKTIKSHHNVGGLPEDLKFRLIEPLRNLFKDEVRQVGFKLGLPEEMINRHPFPGPGLGIRV
IEEVTKDKVEILQEADAIFIKKLRE*NLYNTVSQAFVTLLPVKTVGVMGDNRTYDYVVALRSVNTIDFMTATSTHLP*EF
LDEVVNEIINKVDNVNRVVYDVTSKPPGTIE*E
>Mature_513_residues
MKNTQILILDFGSQYTQLLARRVREANIYTEVLPFDTSIEKIKEYPLLKGIILSGGPSSVYLQNAYKIDSEILNLDIAIL
GVCYGMQLLTQYFQGAVELADEQEFGKAIITIDDKKNPLFKNVTTESQV*MSHADHVTELPVDFKQIAHSNASIAAIAHN
TKPIYGIQFHAEVTHSLQGKEMLENFLYDIAKCNKD*NLDDFIDQQIKEIKETVKDKQVILGLSGGVDSSVAAAIIGKAI
GKQLTCIFVDTGLLRKNETIEVMKAYETNFDINIKLVEASDLFFSELKGIKEPEAKRKIIGKCFIDVFTDAARQHKDADF
LAQGTIYPDVIESSSHGASSKTIKSHHNVGGLPEDLKFRLIEPLRNLFKDEVRQVGFKLGLPEEMINRHPFPGPGLGIRV
IEEVTKDKVEILQEADAIFIKKLRE*NLYNTVSQAFVTLLPVKTVGVMGDNRTYDYVVALRSVNTIDFMTATSTHLP*EF
LDEVVNEIINKVDNVNRVVYDVTSKPPGTIE*E

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=549, Percent_Identity=37.1584699453552, Blast_Score=305, Evalue=7e-83,
Organism=Escherichia coli, GI1788854, Length=518, Percent_Identity=50.7722007722008, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71992717, Length=568, Percent_Identity=35.7394366197183, Blast_Score=294, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI71992710, Length=568, Percent_Identity=35.7394366197183, Blast_Score=294, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI133901714, Length=568, Percent_Identity=35.7394366197183, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6323873, Length=512, Percent_Identity=50.9765625, Blast_Score=505, Evalue=1e-144,
Organism=Drosophila melanogaster, GI281365319, Length=553, Percent_Identity=36.5280289330922, Blast_Score=298, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 56724; Mature: 56724

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTQILILDFGSQYTQLLARRVREANIYTEVLPFDTSIEKIKEYPLLKGIILSGGPSSV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCHHEEEEECCCCCCE
YLQNAYKIDSEILNLDIAILGVCYGMQLLTQYFQGAVELADEQEFGKAIITIDDKKNPLF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCCH
KNVTTESQVMSHADHVTELPVDFKQIAHSNASIAAIAHNTKPIYGIQFHAEVTHSLQGKE
HCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEEHHHHHCCCHHH
MLENFLYDIAKCNKDNLDDFIDQQIKEIKETVKDKQVILGLSGGVDSSVAAAIIGKAIGK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCC
QLTCIFVDTGLLRKNETIEVMKAYETNFDINIKLVEASDLFFSELKGIKEPEAKRKIIGK
CEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECHHHHHHHHCCCCCCHHHHHHHHH
CFIDVFTDAARQHKDADFLAQGTIYPDVIESSSHGASSKTIKSHHNVGGLPEDLKFRLIE
HHHHHHHHHHHHCCCCCHHCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
PLRNLFKDEVRQVGFKLGLPEEMINRHPFPGPGLGIRVIEEVTKDKVEILQEADAIFIKK
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRENLYNTVSQAFVTLLPVKTVGVMGDNRTYDYVVALRSVNTIDFMTATSTHLPEFLDEV
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHH
VNEIINKVDNVNRVVYDVTSKPPGTIEE
HHHHHHHHHCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKNTQILILDFGSQYTQLLARRVREANIYTEVLPFDTSIEKIKEYPLLKGIILSGGPSSV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCHHEEEEECCCCCCE
YLQNAYKIDSEILNLDIAILGVCYGMQLLTQYFQGAVELADEQEFGKAIITIDDKKNPLF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCCH
KNVTTESQVMSHADHVTELPVDFKQIAHSNASIAAIAHNTKPIYGIQFHAEVTHSLQGKE
HCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEEHHHHHCCCHHH
MLENFLYDIAKCNKDNLDDFIDQQIKEIKETVKDKQVILGLSGGVDSSVAAAIIGKAIGK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCC
QLTCIFVDTGLLRKNETIEVMKAYETNFDINIKLVEASDLFFSELKGIKEPEAKRKIIGK
CEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECHHHHHHHHCCCCCCHHHHHHHHH
CFIDVFTDAARQHKDADFLAQGTIYPDVIESSSHGASSKTIKSHHNVGGLPEDLKFRLIE
HHHHHHHHHHHHCCCCCHHCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
PLRNLFKDEVRQVGFKLGLPEEMINRHPFPGPGLGIRVIEEVTKDKVEILQEADAIFIKK
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRENLYNTVSQAFVTLLPVKTVGVMGDNRTYDYVVALRSVNTIDFMTATSTHLPEFLDEV
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHH
VNEIINKVDNVNRVVYDVTSKPPGTIEE
HHHHHHHHHCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA