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The map label for this gene is pnp [C]

Identifier: 50365157

GI number: 50365157

Start: 396680

End: 396916

Strand: Direct

Name: pnp [C]

Synonym: Mfl341

Alternate gene names: 50365157

Gene position: 396680-396916 (Clockwise)

Preceding gene: 50365156

Following gene: 50365158

Centisome position: 50.01

GC content: 28.27

Gene sequence:

>237_bases
ATGAACAAAGTTATCTCAGTTAAAATTTCTGATATTGCTCCTTTTGGTGCATTTGCTATTTTTGAATTAGACGGTAAAGA
ATACAAAGGTTTAATCCATATTAGTGAAATTGCTAACACTTTTGTAAACGACATTAACGATTTCGTTAAAGTTGGTCAAG
ACGTTGAAGTTCTAATTTTAGAATTAAACGATGAAAAAGCTCAAGCAAAATTATCAATTAAAAAAGTTAACGCTTAA

Upstream 100 bases:

>100_bases
TATAATAAAGTTAGTATTTGAACAAATCGAATCTTAAAATTTTAACTTTTAGTTCATAATTTTATCCATTTGATCAGTAT
TATAGAAAGTAGACGACAAA

Downstream 100 bases:

>100_bases
TTAATAATTAAACGAACTAAAAAAGAGAACTTACAGTTCTCTTTTTTTCTTTGCAAATATTTTAACATTTATTGTTGAAT
ATAAATACAACATTAATATA

Product: fused polyribonucleotide nucleotidyltransferase/ribosomal protein S1 domain-containing protein

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 78; Mature: 78

Protein sequence:

>78_residues
MNKVISVKISDIAPFGAFAIFELDGKEYKGLIHISEIANTFVNDINDFVKVGQDVEVLILELNDEKAQAKLSIKKVNA

Sequences:

>Translated_78_residues
MNKVISVKISDIAPFGAFAIFELDGKEYKGLIHISEIANTFVNDINDFVKVGQDVEVLILELNDEKAQAKLSIKKVNA
>Mature_78_residues
MNKVISVKISDIAPFGAFAIFELDGKEYKGLIHISEIANTFVNDINDFVKVGQDVEVLILELNDEKAQAKLSIKKVNA

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 8621; Mature: 8621

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVISVKISDIAPFGAFAIFELDGKEYKGLIHISEIANTFVNDINDFVKVGQDVEVLIL
CCCEEEEEECCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEE
ELNDEKAQAKLSIKKVNA
EECCCCCEEEEEEEECCC
>Mature Secondary Structure
MNKVISVKISDIAPFGAFAIFELDGKEYKGLIHISEIANTFVNDINDFVKVGQDVEVLIL
CCCEEEEEECCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEE
ELNDEKAQAKLSIKKVNA
EECCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA