| Definition | Mesoplasma florum L1, complete genome. |
|---|---|
| Accession | NC_006055 |
| Length | 793,224 |
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The map label for this gene is ribF/mnmA [H]
Identifier: 50365150
GI number: 50365150
Start: 390800
End: 391624
Strand: Reverse
Name: ribF/mnmA [H]
Synonym: Mfl334
Alternate gene names: 50365150
Gene position: 391624-390800 (Counterclockwise)
Preceding gene: 50365152
Following gene: 50365149
Centisome position: 49.37
GC content: 23.52
Gene sequence:
>825_bases ATGACTATACACTATAATGAAATGCTAATGATGCTATGAAATTTGCCTCAAACAGTAGCTGTATTTGCAGAATTTGATAA ATGAGATGAAAATGAAACTAATTTAATTAAAAAAGCGAAAGAGAACAATTTAAAAGTTATTTTGATTCAAAAAGAAATTA GTAACTTTAAAAATATAATTCCAAATAAAGGAATTGAGCAAAAAGCCAAAGATTTAGATTGTGAACTTGTTTTATGACAT TCATTTAATCAAGAATATAATTATGAAAGAATTAAACAAGATTTAAATATTCAAAAAGTTTATTTATTTGATAACAATTC AGATCTAGCCAAAATTGAAACAGCTTTTGAAGATAAAACAATTATTAAATATGATGAAAAACTTAATAATGATATTAAAA AGAGTGAAGAGTTTTTAAAATTAGGAAAAATTCAAGAATATAAAAATTTAAATGGTTTTAATTTTGGTTTTAAAGGTTTT GTTGAGCATGGTAACCACATTGGGAGAACAATTAATTATCCAACTGCAAATATCATTATTAATGATGAGTTGATTTTGAA AGAAGCTGTTTATTTAACAACTACTAAATTACCAAATGACGAAAAAGCATATCCAAGTATTAGCGCTTATTGAACTAATA AAAATGGAGTTAATGTATTTGAGAGTCATATTTTAGATTTTGATCAAGATATTTATGGATGAGGAATCGATGTAGAAATT ATTGATTTTATACGTGAAAGTATTAAAGTAAATAACTTAGACGAACTGAAAGATTTATTAAATCAAGATAAAGCATATGC AATAAAACATTTTAAAGGAGAATAA
Upstream 100 bases:
>100_bases TATAATGTAAAATAGAAATAAATTAATAATTTAGTACAACATTAATTTGAATTTTAAAAATATAATTAAATTAAATTGTA TTAAGTGGGGGAGAAACAAA
Downstream 100 bases:
>100_bases AAAATGAAAAAACCAATTTTAGGTAGCCATGTTGGTATGAGCAAATCAAATAAACATGGAGAATATTTAATCGGAAGTGT AAAAGAAGCTTTAAGTTATG
Product: riboflavin kinase
Products: NA
Alternate protein names: FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase; Riboflavin kinase; Flavokinase; tRNA-specific 2-thiouridylase mnmA [H]
Number of amino acids: Translated: 274; Mature: 273
Protein sequence:
>274_residues MTIHYNEMLMMLWNLPQTVAVFAEFDKWDENETNLIKKAKENNLKVILIQKEISNFKNIIPNKGIEQKAKDLDCELVLWH SFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIKYDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGF VEHGNHIGRTINYPTANIIINDELILKEAVYLTTTKLPNDEKAYPSISAYWTNKNGVNVFESHILDFDQDIYGWGIDVEI IDFIRESIKVNNLDELKDLLNQDKAYAIKHFKGE
Sequences:
>Translated_274_residues MTIHYNEMLMML*NLPQTVAVFAEFDK*DENETNLIKKAKENNLKVILIQKEISNFKNIIPNKGIEQKAKDLDCELVL*H SFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIKYDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGF VEHGNHIGRTINYPTANIIINDELILKEAVYLTTTKLPNDEKAYPSISAY*TNKNGVNVFESHILDFDQDIYG*GIDVEI IDFIRESIKVNNLDELKDLLNQDKAYAIKHFKGE >Mature_273_residues TIHYNEMLMML*NLPQTVAVFAEFDK*DENETNLIKKAKENNLKVILIQKEISNFKNIIPNKGIEQKAKDLDCELVL*HS FNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIKYDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGFV EHGNHIGRTINYPTANIIINDELILKEAVYLTTTKLPNDEKAYPSISAY*TNKNGVNVFESHILDFDQDIYG*GIDVEII DFIRESIKVNNLDELKDLLNQDKAYAIKHFKGE
Specific function: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 [H]
COG id: COG0196
COG function: function code H; FAD synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the mnmA/TRMU family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR015864 - InterPro: IPR015865 - InterPro: IPR002606 - InterPro: IPR014729 - InterPro: IPR004506 [H]
Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase; PF03054 tRNA_Me_trans [H]
EC number: =2.7.7.2; =2.7.1.26 [H]
Molecular weight: Translated: 31182; Mature: 31051
Theoretical pI: Translated: 4.91; Mature: 4.91
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTIHYNEMLMMLNLPQTVAVFAEFDKDENETNLIKKAKENNLKVILIQKEISNFKNIIPN CEEEHHEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCC KGIEQKAKDLDCELVLHSFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIK CCHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCEEEE YDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGFVEHGNHIGRTINYPTANIIINDE ECHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEECCCCEEEEECCH LILKEAVYLTTTKLPNDEKAYPSISAYTNKNGVNVFESHILDFDQDIYGGIDVEIIDFIR HHHHHHEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHH ESIKVNNLDELKDLLNQDKAYAIKHFKGE HHHCCCCHHHHHHHHCCCHHEEHHCCCCC >Mature Secondary Structure TIHYNEMLMMLNLPQTVAVFAEFDKDENETNLIKKAKENNLKVILIQKEISNFKNIIPN EEEHHEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCC KGIEQKAKDLDCELVLHSFNQEYNYERIKQDLNIQKVYLFDNNSDLAKIETAFEDKTIIK CCHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCEEEE YDEKLNNDIKKSEEFLKLGKIQEYKNLNGFNFGFKGFVEHGNHIGRTINYPTANIIINDE ECHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEECCCCEEEEECCH LILKEAVYLTTTKLPNDEKAYPSISAYTNKNGVNVFESHILDFDQDIYGGIDVEIIDFIR HHHHHHEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHH ESIKVNNLDELKDLLNQDKAYAIKHFKGE HHHCCCCHHHHHHHHCCCHHEEHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 12949158 [H]