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The map label for this gene is nfo [H]

Identifier: 50365149

GI number: 50365149

Start: 389915

End: 390796

Strand: Reverse

Name: nfo [H]

Synonym: Mfl333

Alternate gene names: 50365149

Gene position: 390796-389915 (Counterclockwise)

Preceding gene: 50365150

Following gene: 50365148

Centisome position: 49.27

GC content: 26.3

Gene sequence:

>882_bases
ATGAAAAAACCAATTTTAGGTAGCCATGTTGGTATGAGCAAATCAAATAAACATGGAGAATATTTAATCGGAAGTGTAAA
AGAAGCTTTAAGTTATGAAGCTAATACTTTAATGTTTTATACTGGTGCACCACAAAACTCAGTTCGTACTGCAACTGATA
AGTTGTATATTGAAGAATTTAATCAATTATTAAAAGAAAATAATATTAACAAAGATCACATTGTAGTTCATGCACCTTAT
ATTATTAATATTTCAAATCCAGTTAATCAAACAACTTGAGATTTTGGTGTTGATTTTTTAAAACAAGAAATTAAACGTTG
TGAAGATATCGGAGTTAAGATTTTAGTTTTACACCCAGGAGCAAGATTAAAAGGTGATTATACAGATGCTTTAAATGCTT
TAGTAAAAGGATTAAATGCTGTTGCGTCAAGTCAAACTAATGTAATTATTGCATTAGAAACAATGGCTGGAAAAGGTACT
GAAGTTGGTTTATCTTTAAATGATTTTAAATATGTTATTGATAATGTAAACGAACCAGAAAAAGTTGCTGTATGTTTAGA
TACATGTCATATGCATGATGCTGGTTATGATTTTAATAATTGAGAATTAATTAAAGAAGAAATTAATAAAACAATTGGTA
AAGATAAAGTTTTATGTTTCCATTTAAATGATTCAAAAAACCCGTTATTAGCTCATAAAGATAGACATGAGAATATTGGT
TATGGTTACATTGGTTTTGACAATTTATTAAAAGTACTTTGAGATGAAGAATATATAGATATTCCAAAAATTTTAGAAAC
ACCTTATGTAGAAGATAAACCTCCATATAAAGAAGAAATTGAAAACCTAATTAATAAAAATTTTTCCAAAAAAGTTAAAT
AA

Upstream 100 bases:

>100_bases
TATACGTGAAAGTATTAAAGTAAATAACTTAGACGAACTGAAAGATTTATTAAATCAAGATAAAGCATATGCAATAAAAC
ATTTTAAAGGAGAATAAAAA

Downstream 100 bases:

>100_bases
AATATGAGATAATTAATAAATCAAGGGGCAGGTGATATTATGAGTGAACAAAAATTAAGTATGACAAAAAGACGTACATA
TCTAGTTCAAATGTTTTATA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKKPILGSHVGMSKSNKHGEYLIGSVKEALSYEANTLMFYTGAPQNSVRTATDKLYIEEFNQLLKENNINKDHIVVHAPY
IINISNPVNQTTWDFGVDFLKQEIKRCEDIGVKILVLHPGARLKGDYTDALNALVKGLNAVASSQTNVIIALETMAGKGT
EVGLSLNDFKYVIDNVNEPEKVAVCLDTCHMHDAGYDFNNWELIKEEINKTIGKDKVLCFHLNDSKNPLLAHKDRHENIG
YGYIGFDNLLKVLWDEEYIDIPKILETPYVEDKPPYKEEIENLINKNFSKKVK

Sequences:

>Translated_293_residues
MKKPILGSHVGMSKSNKHGEYLIGSVKEALSYEANTLMFYTGAPQNSVRTATDKLYIEEFNQLLKENNINKDHIVVHAPY
IINISNPVNQTT*DFGVDFLKQEIKRCEDIGVKILVLHPGARLKGDYTDALNALVKGLNAVASSQTNVIIALETMAGKGT
EVGLSLNDFKYVIDNVNEPEKVAVCLDTCHMHDAGYDFNN*ELIKEEINKTIGKDKVLCFHLNDSKNPLLAHKDRHENIG
YGYIGFDNLLKVL*DEEYIDIPKILETPYVEDKPPYKEEIENLINKNFSKKVK
>Mature_293_residues
MKKPILGSHVGMSKSNKHGEYLIGSVKEALSYEANTLMFYTGAPQNSVRTATDKLYIEEFNQLLKENNINKDHIVVHAPY
IINISNPVNQTT*DFGVDFLKQEIKRCEDIGVKILVLHPGARLKGDYTDALNALVKGLNAVASSQTNVIIALETMAGKGT
EVGLSLNDFKYVIDNVNEPEKVAVCLDTCHMHDAGYDFNN*ELIKEEINKTIGKDKVLCFHLNDSKNPLLAHKDRHENIG
YGYIGFDNLLKVL*DEEYIDIPKILETPYVEDKPPYKEEIENLINKNFSKKVK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=292, Percent_Identity=27.7397260273973, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17531193, Length=215, Percent_Identity=32.093023255814, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322735, Length=254, Percent_Identity=36.2204724409449, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 32571; Mature: 32571

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00730 AP_NUCLEASE_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPILGSHVGMSKSNKHGEYLIGSVKEALSYEANTLMFYTGAPQNSVRTATDKLYIEEF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
NQLLKENNINKDHIVVHAPYIINISNPVNQTTDFGVDFLKQEIKRCEDIGVKILVLHPGA
HHHHHHCCCCCCEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCC
RLKGDYTDALNALVKGLNAVASSQTNVIIALETMAGKGTEVGLSLNDFKYVIDNVNEPEK
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCHHHHHHHHCCCCCCHH
VAVCLDTCHMHDAGYDFNNELIKEEINKTIGKDKVLCFHLNDSKNPLLAHKDRHENIGYG
EEEEHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCEE
YIGFDNLLKVLDEEYIDIPKILETPYVEDKPPYKEEIENLINKNFSKKVK
EECHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MKKPILGSHVGMSKSNKHGEYLIGSVKEALSYEANTLMFYTGAPQNSVRTATDKLYIEEF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
NQLLKENNINKDHIVVHAPYIINISNPVNQTTDFGVDFLKQEIKRCEDIGVKILVLHPGA
HHHHHHCCCCCCEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCC
RLKGDYTDALNALVKGLNAVASSQTNVIIALETMAGKGTEVGLSLNDFKYVIDNVNEPEK
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCHHHHHHHHCCCCCCHH
VAVCLDTCHMHDAGYDFNNELIKEEINKTIGKDKVLCFHLNDSKNPLLAHKDRHENIGYG
EEEEHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCEE
YIGFDNLLKVLDEEYIDIPKILETPYVEDKPPYKEEIENLINKNFSKKVK
EECHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA