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The map label for this gene is gpsA

Identifier: 50365016

GI number: 50365016

Start: 226738

End: 227742

Strand: Direct

Name: gpsA

Synonym: Mfl200

Alternate gene names: 50365016

Gene position: 226738-227742 (Clockwise)

Preceding gene: 50365015

Following gene: 50365017

Centisome position: 28.58

GC content: 28.36

Gene sequence:

>1005_bases
ATGAGTAAAAAAAATATAACAATTATAGGAACTGGAGCTTACGGAACAGCATTAGCGAATGTATTAGCAGATAATGATAA
CAATGTTATTATGTATGGAATTGTTGAACAACAAGTTGATGATATTAATATTTATCACCAAAACTCAGTTTTCTTTGACA
ATAAAAAAATAAACAAAACAATTAGAGCAACTAATTCTATGGCAGCGGCTTTAGAAAATACAGATATATTAATTTTAGGA
GTTCCTACAGCAGCTATTAAGCACGTGGTTAATGACATTATTAAATATGCTAAAAAACCAATGGATATAATCAATACTGC
AAAAGGTTTAGATGAAGAAAATCTAGGATTACTTTCTGATAAAATAAAAAAATATTTTGAAGGTTCAAATGTAATATCAA
CTTATTCAGCTTTATATGGTCCTTCAATTGCAATTGAAGTAGTTGATCGTCAACCTACAGCTATTATGATAGCTTCAGAA
ACAATTGAAAAAGCTAAAGAATTATGTAATGTATTTTCTAATGAATATTTCTACATGTATCCAACAACAGATATTGCAGG
ATGTGAGATTTCAGCTGCATTAAAAAATGCCATTGCTATTGGTGGTGGAATTCTTAAGGCATATAATGCTGGAGATAATG
CTCATGCAACATTATTAACACTAGGTTTAAATGAAATGTATGAATTTGGAAAACACTTTGGTGCTAAACTTGAAACATTC
TTAAACTTTGCTGGATTAGGTGATTTAATACTTACTGCATCAAGTAAAAAATCAAGAAATTTCAGATTAGGTGAAAGAAT
TGTAGAACTAAATGATGCTAAAAAAGCTTTAGAATCATTCAATTTAACTGTTGAAGGTGTGGAAACAGCCAGAATAGCCC
ATGAAATAGGGGTTAAATACCAAATTAGCATGAATTTTTTTGAAATAATATATAATATTTTATATAATAATGTTAAGCCT
ATTTCACTTTTAAATAATGTCTTTAGAGACGTGAAATTAGTTTAA

Upstream 100 bases:

>100_bases
ACTTTTCATATAAAAGGTTTTTAGAGAATCAAATAAGACTTCAATTTGGTTTTGAAGGTGTACCAATGTCAATTATTTTC
AGGGAAAGGAAATAAGCACT

Downstream 100 bases:

>100_bases
AAAATTAAAGGAGAAATTATATGACAAAAAAAGACTTAATTAACGAAATTATTGCTAATGAAAACATCTCAAAAGTTGAA
TGTGAAGCAGTAGTTAATTC

Product: NAD-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSKKNITIIGTGAYGTALANVLADNDNNVIMYGIVEQQVDDINIYHQNSVFFDNKKINKTIRATNSMAAALENTDILILG
VPTAAIKHVVNDIIKYAKKPMDIINTAKGLDEENLGLLSDKIKKYFEGSNVISTYSALYGPSIAIEVVDRQPTAIMIASE
TIEKAKELCNVFSNEYFYMYPTTDIAGCEISAALKNAIAIGGGILKAYNAGDNAHATLLTLGLNEMYEFGKHFGAKLETF
LNFAGLGDLILTASSKKSRNFRLGERIVELNDAKKALESFNLTVEGVETARIAHEIGVKYQISMNFFEIIYNILYNNVKP
ISLLNNVFRDVKLV

Sequences:

>Translated_334_residues
MSKKNITIIGTGAYGTALANVLADNDNNVIMYGIVEQQVDDINIYHQNSVFFDNKKINKTIRATNSMAAALENTDILILG
VPTAAIKHVVNDIIKYAKKPMDIINTAKGLDEENLGLLSDKIKKYFEGSNVISTYSALYGPSIAIEVVDRQPTAIMIASE
TIEKAKELCNVFSNEYFYMYPTTDIAGCEISAALKNAIAIGGGILKAYNAGDNAHATLLTLGLNEMYEFGKHFGAKLETF
LNFAGLGDLILTASSKKSRNFRLGERIVELNDAKKALESFNLTVEGVETARIAHEIGVKYQISMNFFEIIYNILYNNVKP
ISLLNNVFRDVKLV
>Mature_333_residues
SKKNITIIGTGAYGTALANVLADNDNNVIMYGIVEQQVDDINIYHQNSVFFDNKKINKTIRATNSMAAALENTDILILGV
PTAAIKHVVNDIIKYAKKPMDIINTAKGLDEENLGLLSDKIKKYFEGSNVISTYSALYGPSIAIEVVDRQPTAIMIASET
IEKAKELCNVFSNEYFYMYPTTDIAGCEISAALKNAIAIGGGILKAYNAGDNAHATLLTLGLNEMYEFGKHFGAKLETFL
NFAGLGDLILTASSKKSRNFRLGERIVELNDAKKALESFNLTVEGVETARIAHEIGVKYQISMNFFEIIYNILYNNVKPI
SLLNNVFRDVKLV

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=340, Percent_Identity=28.5294117647059, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI24307999, Length=340, Percent_Identity=26.7647058823529, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1790037, Length=318, Percent_Identity=31.1320754716981, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32564399, Length=352, Percent_Identity=25.8522727272727, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI193210136, Length=361, Percent_Identity=25.4847645429363, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32564403, Length=361, Percent_Identity=25.4847645429363, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17507425, Length=331, Percent_Identity=25.9818731117825, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193210134, Length=338, Percent_Identity=24.2603550295858, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324513, Length=345, Percent_Identity=26.9565217391304, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320181, Length=362, Percent_Identity=24.585635359116, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136204, Length=350, Percent_Identity=27.1428571428571, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136202, Length=350, Percent_Identity=27.1428571428571, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136200, Length=350, Percent_Identity=27.1428571428571, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45551945, Length=294, Percent_Identity=24.8299319727891, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281362270, Length=294, Percent_Identity=24.8299319727891, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI22026922, Length=338, Percent_Identity=24.5562130177515, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648969, Length=282, Percent_Identity=24.113475177305, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_MESFL (Q6F1R6)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053441.1
- ProteinModelPortal:   Q6F1R6
- SMR:   Q6F1R6
- GeneID:   2898285
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl200
- HOGENOM:   HBG586392
- OMA:   SQTLRGN
- ProtClustDB:   CLSK745764
- BioCyc:   MFLO265311:MFL200-MONOMER
- BRENDA:   1.1.1.94
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 36774; Mature: 36643

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 195-195 BINDING 108-108 BINDING 108-108 BINDING 144-144 BINDING 259-259 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKNITIIGTGAYGTALANVLADNDNNVIMYGIVEQQVDDINIYHQNSVFFDNKKINKT
CCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCEEECCCHHHHH
IRATNSMAAALENTDILILGVPTAAIKHVVNDIIKYAKKPMDIINTAKGLDEENLGLLSD
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHH
KIKKYFEGSNVISTYSALYGPSIAIEVVDRQPTAIMIASETIEKAKELCNVFSNEYFYMY
HHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEE
PTTDIAGCEISAALKNAIAIGGGILKAYNAGDNAHATLLTLGLNEMYEFGKHFGAKLETF
CCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
LNFAGLGDLILTASSKKSRNFRLGERIVELNDAKKALESFNLTVEGVETARIAHEIGVKY
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCEE
QISMNFFEIIYNILYNNVKPISLLNNVFRDVKLV
EEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKNITIIGTGAYGTALANVLADNDNNVIMYGIVEQQVDDINIYHQNSVFFDNKKINKT
CCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCEEECCCHHHHH
IRATNSMAAALENTDILILGVPTAAIKHVVNDIIKYAKKPMDIINTAKGLDEENLGLLSD
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHH
KIKKYFEGSNVISTYSALYGPSIAIEVVDRQPTAIMIASETIEKAKELCNVFSNEYFYMY
HHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEE
PTTDIAGCEISAALKNAIAIGGGILKAYNAGDNAHATLLTLGLNEMYEFGKHFGAKLETF
CCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
LNFAGLGDLILTASSKKSRNFRLGERIVELNDAKKALESFNLTVEGVETARIAHEIGVKY
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCEE
QISMNFFEIIYNILYNNVKPISLLNNVFRDVKLV
EEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA