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The map label for this gene is rsmB [H]

Identifier: 50365012

GI number: 50365012

Start: 222691

End: 223968

Strand: Direct

Name: rsmB [H]

Synonym: Mfl196

Alternate gene names: 50365012

Gene position: 222691-223968 (Clockwise)

Preceding gene: 50365011

Following gene: 50365013

Centisome position: 28.07

GC content: 23.47

Gene sequence:

>1278_bases
GTGATTTTAGTGGAGACTAATAATTCAAGAAAAATAGCATTTGAAATTTTGAAAAAAGTATTTAAAAATAAATCTTTTTC
AAACATTCTTTTGAATGATGTTTCAAAAATGCCAATTTCTGACAAGTTTAAGAACTTAATTTTTGCAATAGTTCATGGAA
CTATAACAAATCAAATTTTACTTGACCATGTTTCAAGAAAATTGATTGATGTTAAAAAAACTAATATTGATATTCAAATA
CTTTTATGAATGAGTATTTATCAATTACGTTTTTTAAAAACTATCCCTCAATATGCAGTTGTTAATGAATCGGTTATGAT
TGCAAAGTCTGTTAATCATAAATTTTCCGGTTTATTGAATGCTTGTTTGAAAAAAGTTATTAAAAATGAGGAAGAACTTT
TTGATTTTTCTTTTAAAAGTGAAAATCAAAAAATATGTATTGAAAATTCTTTTCCAAAAAATTTATTTAATTTAATAGAA
AAAGGATATGGAAAAGATATTGCTAAAAAAGTAGCTATTGATTCAAACCAAAAACCTGTTATAAGTTTTAGAGTAAACAC
TTTAAAGATTTCAGAAAATGATTTTTTTGAAAAATATAAAGATGAATATGAATTAGAAAAAACAGAAATAAAAAATTGCT
TGATCTCAAAAAAAGCAATTGTAAAAAGTGACGCTTATACAAGTGGTCAAATAACCATTCAAGACCCAGCATCAATTTTA
GTAGGAAATGTCTTAAATCCAACTAAAGGTTCAAAAGTTTTAGACATGTGTAGCGCACCGGGTGGTAAACTTACTCATTT
AAGTATGATAATGGAAAACACAGGACAAGTAACAGGATTTGAAATAAGTGAAAACAAGATAAAATTAATTGAAGAAAATA
TTAAAAGATTAGATTGTAAAAATATTTCATTAAAGTGTGGAGATGCAACTTTAATTAATGAAAAAGAAAATTTTGATTTT
ATCTTATTAGATGCACCTTGTTCTGGATTTGGTGTTTTAAAAAGAAAACCTGAAATTAAAATTAATAATATTGAAATAAA
TAATATAAATAACATTGTAAATATTCAAGAAAAGTTATTAGATACAGCATATTATAATTTAAAACAACAAGGTGTTATGG
TTTATTCTACTTGTACTATAAATCCTGCTGAAAATCAAAATCAAATTAAAAAGTTTATTGAAAAATATAAAGATATAAAA
ATTATTAGCGAAAACCAATTGTTTGGTTATGAAATGAATACTGATGGTTTTTACATCTGTAAAATGATTAAAGAATAG

Upstream 100 bases:

>100_bases
GCACATGGAGATTTTTCAAAAATTAATGATGTAGAGTATGTAGATTTTAAAATTCAAAAATTAATGTTCAAAATTAACTT
TTTCAGTATAAAACAAAGAA

Downstream 100 bases:

>100_bases
AAGGATTGCCCTTTTATTTTTTTGTTGTTTAAGATTAAAATAAGTATTTGTTATAAAATATATATAAAAGGAGGAAGTAA
CATGGACAAACAATTATCAA

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MILVETNNSRKIAFEILKKVFKNKSFSNILLNDVSKMPISDKFKNLIFAIVHGTITNQILLDHVSRKLIDVKKTNIDIQI
LLWMSIYQLRFLKTIPQYAVVNESVMIAKSVNHKFSGLLNACLKKVIKNEEELFDFSFKSENQKICIENSFPKNLFNLIE
KGYGKDIAKKVAIDSNQKPVISFRVNTLKISENDFFEKYKDEYELEKTEIKNCLISKKAIVKSDAYTSGQITIQDPASIL
VGNVLNPTKGSKVLDMCSAPGGKLTHLSMIMENTGQVTGFEISENKIKLIEENIKRLDCKNISLKCGDATLINEKENFDF
ILLDAPCSGFGVLKRKPEIKINNIEINNINNIVNIQEKLLDTAYYNLKQQGVMVYSTCTINPAENQNQIKKFIEKYKDIK
IISENQLFGYEMNTDGFYICKMIKE

Sequences:

>Translated_425_residues
MILVETNNSRKIAFEILKKVFKNKSFSNILLNDVSKMPISDKFKNLIFAIVHGTITNQILLDHVSRKLIDVKKTNIDIQI
LL*MSIYQLRFLKTIPQYAVVNESVMIAKSVNHKFSGLLNACLKKVIKNEEELFDFSFKSENQKICIENSFPKNLFNLIE
KGYGKDIAKKVAIDSNQKPVISFRVNTLKISENDFFEKYKDEYELEKTEIKNCLISKKAIVKSDAYTSGQITIQDPASIL
VGNVLNPTKGSKVLDMCSAPGGKLTHLSMIMENTGQVTGFEISENKIKLIEENIKRLDCKNISLKCGDATLINEKENFDF
ILLDAPCSGFGVLKRKPEIKINNIEINNINNIVNIQEKLLDTAYYNLKQQGVMVYSTCTINPAENQNQIKKFIEKYKDIK
IISENQLFGYEMNTDGFYICKMIKE
>Mature_425_residues
MILVETNNSRKIAFEILKKVFKNKSFSNILLNDVSKMPISDKFKNLIFAIVHGTITNQILLDHVSRKLIDVKKTNIDIQI
LL*MSIYQLRFLKTIPQYAVVNESVMIAKSVNHKFSGLLNACLKKVIKNEEELFDFSFKSENQKICIENSFPKNLFNLIE
KGYGKDIAKKVAIDSNQKPVISFRVNTLKISENDFFEKYKDEYELEKTEIKNCLISKKAIVKSDAYTSGQITIQDPASIL
VGNVLNPTKGSKVLDMCSAPGGKLTHLSMIMENTGQVTGFEISENKIKLIEENIKRLDCKNISLKCGDATLINEKENFDF
ILLDAPCSGFGVLKRKPEIKINNIEINNINNIVNIQEKLLDTAYYNLKQQGVMVYSTCTINPAENQNQIKKFIEKYKDIK
IISENQLFGYEMNTDGFYICKMIKE

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI32698918, Length=208, Percent_Identity=35.0961538461538, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI76150625, Length=287, Percent_Identity=27.5261324041812, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI76150623, Length=287, Percent_Identity=27.5261324041812, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI8922322, Length=241, Percent_Identity=24.0663900414938, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI270288818, Length=241, Percent_Identity=24.0663900414938, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI270288816, Length=241, Percent_Identity=24.0663900414938, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI23199998, Length=241, Percent_Identity=24.0663900414938, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI39995082, Length=185, Percent_Identity=30.8108108108108, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI301336155, Length=185, Percent_Identity=30.8108108108108, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI11545785, Length=173, Percent_Identity=29.4797687861272, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=446, Percent_Identity=28.2511210762332, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87081985, Length=247, Percent_Identity=33.1983805668016, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71998419, Length=172, Percent_Identity=33.7209302325581, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115534021, Length=172, Percent_Identity=33.7209302325581, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=296, Percent_Identity=26.6891891891892, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25143471, Length=194, Percent_Identity=29.8969072164948, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=238, Percent_Identity=31.5126050420168, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319447, Length=190, Percent_Identity=28.4210526315789, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324306, Length=127, Percent_Identity=33.8582677165354, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI22024126, Length=228, Percent_Identity=34.2105263157895, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21355201, Length=186, Percent_Identity=29.5698924731183, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356579, Length=185, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 48349; Mature: 48349

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVETNNSRKIAFEILKKVFKNKSFSNILLNDVSKMPISDKFKNLIFAIVHGTITNQIL
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LDHVSRKLIDVKKTNIDIQILLMSIYQLRFLKTIPQYAVVNESVMIAKSVNHKFSGLLNA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCHHHHHHHHH
CLKKVIKNEEELFDFSFKSENQKICIENSFPKNLFNLIEKGYGKDIAKKVAIDSNQKPVI
HHHHHHCCHHHHEEEEECCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEE
SFRVNTLKISENDFFEKYKDEYELEKTEIKNCLISKKAIVKSDAYTSGQITIQDPASILV
EEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCHHHHC
GNVLNPTKGSKVLDMCSAPGGKLTHLSMIMENTGQVTGFEISENKIKLIEENIKRLDCKN
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
ISLKCGDATLINEKENFDFILLDAPCSGFGVLKRKPEIKINNIEINNINNIVNIQEKLLD
CEEEECCEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHH
TAYYNLKQQGVMVYSTCTINPAENQNQIKKFIEKYKDIKIISENQLFGYEMNTDGFYICK
HHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEE
MIKE
EECC
>Mature Secondary Structure
MILVETNNSRKIAFEILKKVFKNKSFSNILLNDVSKMPISDKFKNLIFAIVHGTITNQIL
CEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LDHVSRKLIDVKKTNIDIQILLMSIYQLRFLKTIPQYAVVNESVMIAKSVNHKFSGLLNA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCHHHHHHHHH
CLKKVIKNEEELFDFSFKSENQKICIENSFPKNLFNLIEKGYGKDIAKKVAIDSNQKPVI
HHHHHHCCHHHHEEEEECCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEE
SFRVNTLKISENDFFEKYKDEYELEKTEIKNCLISKKAIVKSDAYTSGQITIQDPASILV
EEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCHHHHC
GNVLNPTKGSKVLDMCSAPGGKLTHLSMIMENTGQVTGFEISENKIKLIEENIKRLDCKN
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
ISLKCGDATLINEKENFDFILLDAPCSGFGVLKRKPEIKINNIEINNINNIVNIQEKLLD
CEEEECCEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHH
TAYYNLKQQGVMVYSTCTINPAENQNQIKKFIEKYKDIKIISENQLFGYEMNTDGFYICK
HHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEE
MIKE
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]