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The map label for this gene is gmk [H]

Identifier: 50365011

GI number: 50365011

Start: 221817

End: 222710

Strand: Direct

Name: gmk [H]

Synonym: Mfl195

Alternate gene names: 50365011

Gene position: 221817-222710 (Clockwise)

Preceding gene: 50365010

Following gene: 50365012

Centisome position: 27.96

GC content: 26.17

Gene sequence:

>894_bases
ATGAAGAATAAAGGAAAAGTAATTATTATATCAGGACCAAGTGGTGTAGGAAAAGGGAGTGTTAATGGTGAACTACTTAC
TAACAAAGATTTAAAATTAGAATATTCAGTTTCTATGACAACAAGAGCACCTCGTGAAGGTGAAGTTAATGGTGTTAATT
ACTTTTTTGTTTCAAAAGAAGAATTTGCTAATGCAATAGTAAACAATGAGTTAATTGAATATGCAAATTTTGTAGGTAAC
TCATATGGAACACCTAGAAAATATGTAGAAGAAAAACTTAATGAAGGTAAAAACGTTATTTTAGAAATTGAAGTGGATGG
TGCCACTCAAGTTTTAAGAAATGAAGAAAATGTTTTATCTATATTTTTAATGCCACCAACATTAAATGAATTAGAATCAC
GAATTAAAGGAAGAGCAACAGAGTCTGATGACAAAATTAAAGCTCGTTTAGATAAAGCTTTATTAGAAATACCTTTAAAA
CATAATTATGATTATGTTGTTGAAAATGATTCTGTTGAAAATGCAGTTTCTAAAATTACTGATATTTTAATTAGAGAAAA
ATGTACTGAATCTAATGAAGAATCTAAATTTAAAGAATTAGTTAAAATCGTTGAAAACATTGTTGATCAAAAATATACTT
ATTTTATAAATAATTGAGAAGCAAACGTTAAATTGTTAGCACATAACAAAGAAGCTAAAAAAGAAGCAAACGATTTTGAT
GCTAGAGGAGAATTGATTAAAATTTTATCTTCTGAAGTTTATAGAAAAACTCTAGCACATGGAGATTTTTCAAAAATTAA
TGATGTAGAGTATGTAGATTTTAAAATTCAAAAATTAATGTTCAAAATTAACTTTTTCAGTATAAAACAAAGAAGTGATT
TTAGTGGAGACTAA

Upstream 100 bases:

>100_bases
GAAATGCCTTTAAATGAAGAAGTTATTTCGCAATTTAAACTATTAAAATACAAAGATTACAATAAAAAACATCTATACAT
TATAAGATTGGAGAACAAAG

Downstream 100 bases:

>100_bases
TAATTCAAGAAAAATAGCATTTGAAATTTTGAAAAAAGTATTTAAAAATAAATCTTTTTCAAACATTCTTTTGAATGATG
TTTCAAAAATGCCAATTTCT

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKNKGKVIIISGPSGVGKGSVNGELLTNKDLKLEYSVSMTTRAPREGEVNGVNYFFVSKEEFANAIVNNELIEYANFVGN
SYGTPRKYVEEKLNEGKNVILEIEVDGATQVLRNEENVLSIFLMPPTLNELESRIKGRATESDDKIKARLDKALLEIPLK
HNYDYVVENDSVENAVSKITDILIREKCTESNEESKFKELVKIVENIVDQKYTYFINNWEANVKLLAHNKEAKKEANDFD
ARGELIKILSSEVYRKTLAHGDFSKINDVEYVDFKIQKLMFKINFFSIKQRSDFSGD

Sequences:

>Translated_297_residues
MKNKGKVIIISGPSGVGKGSVNGELLTNKDLKLEYSVSMTTRAPREGEVNGVNYFFVSKEEFANAIVNNELIEYANFVGN
SYGTPRKYVEEKLNEGKNVILEIEVDGATQVLRNEENVLSIFLMPPTLNELESRIKGRATESDDKIKARLDKALLEIPLK
HNYDYVVENDSVENAVSKITDILIREKCTESNEESKFKELVKIVENIVDQKYTYFINN*EANVKLLAHNKEAKKEANDFD
ARGELIKILSSEVYRKTLAHGDFSKINDVEYVDFKIQKLMFKINFFSIKQRSDFSGD
>Mature_297_residues
MKNKGKVIIISGPSGVGKGSVNGELLTNKDLKLEYSVSMTTRAPREGEVNGVNYFFVSKEEFANAIVNNELIEYANFVGN
SYGTPRKYVEEKLNEGKNVILEIEVDGATQVLRNEENVLSIFLMPPTLNELESRIKGRATESDDKIKARLDKALLEIPLK
HNYDYVVENDSVENAVSKITDILIREKCTESNEESKFKELVKIVENIVDQKYTYFINN*EANVKLLAHNKEAKKEANDFD
ARGELIKILSSEVYRKTLAHGDFSKINDVEYVDFKIQKLMFKINFFSIKQRSDFSGD

Specific function: Essential for recycling GMP and indirectly, cGMP [H]

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain [H]

Homologues:

Organism=Homo sapiens, GI226874833, Length=184, Percent_Identity=32.0652173913043, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4504221, Length=184, Percent_Identity=32.0652173913043, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI226874831, Length=184, Percent_Identity=32.0652173913043, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI14780902, Length=187, Percent_Identity=27.807486631016, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI186700629, Length=183, Percent_Identity=29.5081967213115, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI186700627, Length=183, Percent_Identity=29.5081967213115, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI186972120, Length=183, Percent_Identity=29.5081967213115, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI262118263, Length=178, Percent_Identity=29.2134831460674, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4505237, Length=178, Percent_Identity=29.2134831460674, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI262118255, Length=178, Percent_Identity=29.2134831460674, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118259, Length=178, Percent_Identity=29.2134831460674, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI261862237, Length=181, Percent_Identity=30.3867403314917, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI119943108, Length=181, Percent_Identity=30.3867403314917, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI38570142, Length=132, Percent_Identity=31.0606060606061, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21361598, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI119943106, Length=180, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4557529, Length=182, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI192447426, Length=181, Percent_Identity=30.939226519337, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790080, Length=199, Percent_Identity=36.6834170854271, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17508835, Length=186, Percent_Identity=36.5591397849462, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI193203132, Length=186, Percent_Identity=36.5591397849462, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71988041, Length=176, Percent_Identity=26.7045454545455, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71988048, Length=178, Percent_Identity=26.4044943820225, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557440, Length=183, Percent_Identity=27.3224043715847, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71984092, Length=197, Percent_Identity=30.4568527918782, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320662, Length=185, Percent_Identity=39.4594594594595, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI21356141, Length=184, Percent_Identity=34.7826086956522, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI85816281, Length=132, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI85816106, Length=132, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45551002, Length=132, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362230, Length=188, Percent_Identity=26.063829787234, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648808, Length=188, Percent_Identity=26.063829787234, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640542, Length=174, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45554489, Length=174, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007136, Length=174, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161077626, Length=174, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161078487, Length=127, Percent_Identity=31.496062992126, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161078485, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648810, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281362232, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI161077752, Length=183, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665 [H]

Pfam domain/function: PF00625 Guanylate_kin [H]

EC number: =2.7.4.8 [H]

Molecular weight: Translated: 33623; Mature: 33623

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00856 GUANYLATE_KINASE_1 ; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKGKVIIISGPSGVGKGSVNGELLTNKDLKLEYSVSMTTRAPREGEVNGVNYFFVSKE
CCCCCCEEEEECCCCCCCCCCCCCEEECCCEEEEEEEEEEECCCCCCCCCCEEEEEEEHH
EFANAIVNNELIEYANFVGNSYGTPRKYVEEKLNEGKNVILEIEVDGATQVLRNEENVLS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCEEE
IFLMPPTLNELESRIKGRATESDDKIKARLDKALLEIPLKHNYDYVVENDSVENAVSKIT
EEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
DILIREKCTESNEESKFKELVKIVENIVDQKYTYFINNEANVKLLAHNKEAKKEANDFDA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHCCCCCH
RGELIKILSSEVYRKTLAHGDFSKINDVEYVDFKIQKLMFKINFFSIKQRSDFSGD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHEEEEEECCCCCCCC
>Mature Secondary Structure
MKNKGKVIIISGPSGVGKGSVNGELLTNKDLKLEYSVSMTTRAPREGEVNGVNYFFVSKE
CCCCCCEEEEECCCCCCCCCCCCCEEECCCEEEEEEEEEEECCCCCCCCCCEEEEEEEHH
EFANAIVNNELIEYANFVGNSYGTPRKYVEEKLNEGKNVILEIEVDGATQVLRNEENVLS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCEEE
IFLMPPTLNELESRIKGRATESDDKIKARLDKALLEIPLKHNYDYVVENDSVENAVSKIT
EEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
DILIREKCTESNEESKFKELVKIVENIVDQKYTYFINNEANVKLLAHNKEAKKEANDFDA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHCCCCCH
RGELIKILSSEVYRKTLAHGDFSKINDVEYVDFKIQKLMFKINFFSIKQRSDFSGD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA