Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 50364978

GI number: 50364978

Start: 185273

End: 186712

Strand: Direct

Name: gatB [H]

Synonym: Mfl163

Alternate gene names: 50364978

Gene position: 185273-186712 (Clockwise)

Preceding gene: 50364977

Following gene: 50364981

Centisome position: 23.36

GC content: 25.76

Gene sequence:

>1440_bases
ATGAGAAATTTTGAAATAGTTATCGGTATTGAAAATCATGTTGAATTAAAAACTAAATCTAAGATGTTTTCATCTGCTCC
AGTAAGTTATGGAGAAACTCCAAATACAAATGTTAATGAAACTGATATGGCTTACCCTGGAAGTTTACCTACAATTAACA
AAAAGGGAATTGAATTAGCAATTAGAACTTGTAATGCTTTAAATATGGAAATAGATACTTTAGTTAAATTTGATCGTAAG
AATTATTTTTACCCTGATTTAACAAAAGGATACCAAATCACACAACAATATAATCCAATTGGTAAAAACGGAAAGTTAAA
TATTAATGTTAATGGTCTAACAAAAGAAGTTGATATTGAAAGACTTCACATGGAAGAAGACACAGCAAAACAAATTCATA
AAGATGACTTAACATATATTGACTATAATAGAGCTGGAACAGGTTTAGTTGAAATTGTTACAAGACCAGTTTTAAGAAGT
GCTGATGAAGCTTGTGCTTATGTAGAGAAATTAAGAGAAGTTTTACTATTTTTAAAAGTAAGTGATGTAAAAATGAATGA
AGGTAGTTTAAGAACTGATGTTAACATTTCTATTAGACCTTTTGGAACTCAAGAATTTTCTAATAAAGTAGAAGTTAAAA
ACCTAAATTCAATTTCAAATATTAAAAAAGCAATTGAATTCGAAGTTGAACGTCAAACAAAATTAATGTTAAATAATGAA
ATTATTATTCAAGAAACTCGTAGATTTGATGACACAACAAATTCAACAGTTTCAATGCGTAGCAAATCTGATGCTTTAGA
TTATAAATATTTTAGAGAACCAAATATTATGCCAATTCAATTAAAAAAAGAATGAGTTGAAGATTGTATTAAGAATTCAC
CTGAGTTAGCTGATATTAAAAGAATTAAATATGTTAATGATTATAAAATATCAATTAATGATGCAAACATCATTTTAACT
AGTATTGAAATGACTGAATTTTTTGAAGAAACTATTAAATTTACAAACAACTATACAAAAGTTGCAAATATTTTAATTAG
TGACATTCAAGCGCAATTAAATAATGAAAATACAACAATTGATAAATTAGCATTATTGCCATCTCATTTGGCTGAAATGA
TTAATTTAGTTGATCAATCAGTTATATCATCAAAACATACAAAAACAATTTTGCCAATCATTATGAAAGACTCATCAAAA
TCAGTTATAGAAATAGTTGAAGAGTTAAACATAAAAATGATTAGTGATGAAAATGAAATAGCTAATTTAGTTAATCCAAT
TATTGAAAGCAATTTAGAATTGCTTGAACAATATTCTGAAAGACCAGAAAGAGTTACAAAAACAATAATGGGTCAATTAA
TGAAAGTAACTGGTGGTAATGTAAATCCAGAAGCTGGTATGAATATCATTATTAAATTAGTTGAAAATAAAATAAAGTAA

Upstream 100 bases:

>100_bases
AATATAAATGCAAAACCAAAAGAAGATCTATTATGTTTACAAGCAGCTAAATTACTTGAAGATATAATTGGTATTAAAAA
CGAAATAGTGGAGGATTAAG

Downstream 100 bases:

>100_bases
AAAAAATGAAGCGAGCGCTTCATTTTTCTTTTAAAATTATTTTCTAACGATTGGCCCAGTTAAAACTAAGAAACCTAAGA
TTATAACAACATAGAAACCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRNFEIVIGIENHVELKTKSKMFSSAPVSYGETPNTNVNETDMAYPGSLPTINKKGIELAIRTCNALNMEIDTLVKFDRK
NYFYPDLTKGYQITQQYNPIGKNGKLNINVNGLTKEVDIERLHMEEDTAKQIHKDDLTYIDYNRAGTGLVEIVTRPVLRS
ADEACAYVEKLREVLLFLKVSDVKMNEGSLRTDVNISIRPFGTQEFSNKVEVKNLNSISNIKKAIEFEVERQTKLMLNNE
IIIQETRRFDDTTNSTVSMRSKSDALDYKYFREPNIMPIQLKKEWVEDCIKNSPELADIKRIKYVNDYKISINDANIILT
SIEMTEFFEETIKFTNNYTKVANILISDIQAQLNNENTTIDKLALLPSHLAEMINLVDQSVISSKHTKTILPIIMKDSSK
SVIEIVEELNIKMISDENEIANLVNPIIESNLELLEQYSERPERVTKTIMGQLMKVTGGNVNPEAGMNIIIKLVENKIK

Sequences:

>Translated_479_residues
MRNFEIVIGIENHVELKTKSKMFSSAPVSYGETPNTNVNETDMAYPGSLPTINKKGIELAIRTCNALNMEIDTLVKFDRK
NYFYPDLTKGYQITQQYNPIGKNGKLNINVNGLTKEVDIERLHMEEDTAKQIHKDDLTYIDYNRAGTGLVEIVTRPVLRS
ADEACAYVEKLREVLLFLKVSDVKMNEGSLRTDVNISIRPFGTQEFSNKVEVKNLNSISNIKKAIEFEVERQTKLMLNNE
IIIQETRRFDDTTNSTVSMRSKSDALDYKYFREPNIMPIQLKKE*VEDCIKNSPELADIKRIKYVNDYKISINDANIILT
SIEMTEFFEETIKFTNNYTKVANILISDIQAQLNNENTTIDKLALLPSHLAEMINLVDQSVISSKHTKTILPIIMKDSSK
SVIEIVEELNIKMISDENEIANLVNPIIESNLELLEQYSERPERVTKTIMGQLMKVTGGNVNPEAGMNIIIKLVENKIK
>Mature_479_residues
MRNFEIVIGIENHVELKTKSKMFSSAPVSYGETPNTNVNETDMAYPGSLPTINKKGIELAIRTCNALNMEIDTLVKFDRK
NYFYPDLTKGYQITQQYNPIGKNGKLNINVNGLTKEVDIERLHMEEDTAKQIHKDDLTYIDYNRAGTGLVEIVTRPVLRS
ADEACAYVEKLREVLLFLKVSDVKMNEGSLRTDVNISIRPFGTQEFSNKVEVKNLNSISNIKKAIEFEVERQTKLMLNNE
IIIQETRRFDDTTNSTVSMRSKSDALDYKYFREPNIMPIQLKKE*VEDCIKNSPELADIKRIKYVNDYKISINDANIILT
SIEMTEFFEETIKFTNNYTKVANILISDIQAQLNNENTTIDKLALLPSHLAEMINLVDQSVISSKHTKTILPIIMKDSSK
SVIEIVEELNIKMISDENEIANLVNPIIESNLELLEQYSERPERVTKTIMGQLMKVTGGNVNPEAGMNIIIKLVENKIK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=484, Percent_Identity=33.4710743801653, Blast_Score=256, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI115534094, Length=351, Percent_Identity=36.4672364672365, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=28.4584980237154, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=30.1810865191147, Blast_Score=238, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54501; Mature: 54501

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFEIVIGIENHVELKTKSKMFSSAPVSYGETPNTNVNETDMAYPGSLPTINKKGIELA
CCCEEEEEEECCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
IRTCNALNMEIDTLVKFDRKNYFYPDLTKGYQITQQYNPIGKNGKLNINVNGLTKEVDIE
EEECCCCCCCHHHHHHCCCCCCCCCCCCCCEEEHHCCCCCCCCCEEEEEECCCEEECCHH
RLHMEEDTAKQIHKDDLTYIDYNRAGTGLVEIVTRPVLRSADEACAYVEKLREVLLFLKV
HHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEE
SDVKMNEGSLRTDVNISIRPFGTQEFSNKVEVKNLNSISNIKKAIEFEVERQTKLMLNNE
CCEEECCCCEEEEEEEEEEECCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHEEECCE
IIIQETRRFDDTTNSTVSMRSKSDALDYKYFREPNIMPIQLKKEVEDCIKNSPELADIKR
EEEEEHHCCCCCCCCEEEECCCCCCCCHHEECCCCEEEEEHHHHHHHHHCCCCCHHHHHH
IKYVNDYKISINDANIILTSIEMTEFFEETIKFTNNYTKVANILISDIQAQLNNENTTID
HEEECCEEEEEECCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
KLALLPSHLAEMINLVDQSVISSKHTKTILPIIMKDSSKSVIEIVEELNIKMISDENEIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCEEEECCCHHHH
NLVNPIIESNLELLEQYSERPERVTKTIMGQLMKVTGGNVNPEAGMNIIIKLVENKIK
HHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRNFEIVIGIENHVELKTKSKMFSSAPVSYGETPNTNVNETDMAYPGSLPTINKKGIELA
CCCEEEEEEECCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
IRTCNALNMEIDTLVKFDRKNYFYPDLTKGYQITQQYNPIGKNGKLNINVNGLTKEVDIE
EEECCCCCCCHHHHHHCCCCCCCCCCCCCCEEEHHCCCCCCCCCEEEEEECCCEEECCHH
RLHMEEDTAKQIHKDDLTYIDYNRAGTGLVEIVTRPVLRSADEACAYVEKLREVLLFLKV
HHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEE
SDVKMNEGSLRTDVNISIRPFGTQEFSNKVEVKNLNSISNIKKAIEFEVERQTKLMLNNE
CCEEECCCCEEEEEEEEEEECCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHEEECCE
IIIQETRRFDDTTNSTVSMRSKSDALDYKYFREPNIMPIQLKKEVEDCIKNSPELADIKR
EEEEEHHCCCCCCCCEEEECCCCCCCCHHEECCCCEEEEEHHHHHHHHHCCCCCHHHHHH
IKYVNDYKISINDANIILTSIEMTEFFEETIKFTNNYTKVANILISDIQAQLNNENTTID
HEEECCEEEEEECCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
KLALLPSHLAEMINLVDQSVISSKHTKTILPIIMKDSSKSVIEIVEELNIKMISDENEIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHCCEEEECCCHHHH
NLVNPIIESNLELLEQYSERPERVTKTIMGQLMKVTGGNVNPEAGMNIIIKLVENKIK
HHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA