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The map label for this gene is gatA [H]

Identifier: 50364977

GI number: 50364977

Start: 183823

End: 185271

Strand: Direct

Name: gatA [H]

Synonym: Mfl162

Alternate gene names: 50364977

Gene position: 183823-185271 (Clockwise)

Preceding gene: 50364976

Following gene: 50364978

Centisome position: 23.17

GC content: 26.98

Gene sequence:

>1449_bases
ATGAATTACAAAAATTTATCAATTTTAGAATTACATAATAAAATTATTAATAAAGAAATAACTATTTTAGAATTAACTAA
TGCAGTAATATCTGAAGCTAATAAAGTTAAATCGTCTAATGCTATAAACAAAATGAATGAAGAAAATGCTATTGCTTTTT
CTAAAGAAATGGAAAAAGTTTTAAACACAAATTCTTTATTGTATGGAATCCCTTATTTTGCAAAAGATAACTTTGCAACA
AAAGGACTTGAAACAACTTCTTCATCTAATATTTTAAAAGGATATATTCCACCATTTAATGCAACTTCTATTTCAAAATT
AGAAGATAAAGGAGCTGTTTTGATTGGTAAAGCTGCTCTTGATGAATTAGGAATGGGTGGGGTTGGACTTTATTCATGTA
ATGGAATAATAACAAATCCTGCAGATAGCAATAGAATTGTTGGTGGAAGTAGTAGTGGTAGTGCTTATTTAGTAGCAAAA
GGTATCGTTCCATTTGCAACAGGTAGTGATACTGGGGATTCAATCAGAAAGCCGGCTTCTTTTAATAACATAGTTGGTTT
TAAACCATCATATGGAGCAATTAGTAGATATGGTTTATTACCTTATTCTCCAAGTTTAGATACTGTTGGTTTCTTTACTA
GAAATGTAGAAGATATGGCAATAGTTTGTGATGCTACTTTTGGTTATGATGTTAAAGATGCAACAAGTCATGAAAATAAA
TCTATAGATATGTTAAATAATATTTCTAATATTAATAAATCAGTTAAATTTGGATATATTAAAAAAGTTATTGATGATTT
AAATGAAAAAGTAAAAAATTCATATTACCAGTTATTTGAAACTTTAAAATCAAAAGGGTATGAAGTAGTTGAGGTTCAGT
TTAACCAAGAATTACTAGATTCATTATCAGCTATTTATAAAATGATTTCATTTAGTGAATCTGTTTCAACTAATGCAAAC
TTAGATGGTATTAAATTTGGACAAAGAGCAGCTGGTTCTGATTATGTTGAAATAATCACTAATTCAAGAACTGAAGGTTT
TGGTAATGAAGTTAAACAAAGATTCCTTGTTGGAGCATTAAATTTAAACAAAGAAAATCAAACAATTTATTTAAACAAAG
CTAAACAAGTAAGAAGATTAATTGTAGAAGAATTAAATAGAGTATTTAGTAAAACTGATATTTTAATTATTCCCCCAACT
GATAATGTAGCTCCATTAATTGAGGAAAGAAAAATTAATGTAAGTCCTGAAAAAGAATACTTAAGTGATATATTAACTTT
AGCAAACTTCAATGGTTCACCATCAATCACAATTCCTTTTGTAAAAGAAGGAAAACTTGGTATAGGAATTAATATAAATG
CAAAACCAAAAGAAGATCTATTATGTTTACAAGCAGCTAAATTACTTGAAGATATAATTGGTATTAAAAACGAAATAGTG
GAGGATTAA

Upstream 100 bases:

>100_bases
GAGAAGATGAAGAAACAACAACTATAGATCAAACAATAATATTGTCAAATGCACCAACTTCAGAGGGTGATTTTGTCACA
ATCAAAAAGGTGGTTAAATA

Downstream 100 bases:

>100_bases
GATGAGAAATTTTGAAATAGTTATCGGTATTGAAAATCATGTTGAATTAAAAACTAAATCTAAGATGTTTTCATCTGCTC
CAGTAAGTTATGGAGAAACT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNYKNLSILELHNKIINKEITILELTNAVISEANKVKSSNAINKMNEENAIAFSKEMEKVLNTNSLLYGIPYFAKDNFAT
KGLETTSSSNILKGYIPPFNATSISKLEDKGAVLIGKAALDELGMGGVGLYSCNGIITNPADSNRIVGGSSSGSAYLVAK
GIVPFATGSDTGDSIRKPASFNNIVGFKPSYGAISRYGLLPYSPSLDTVGFFTRNVEDMAIVCDATFGYDVKDATSHENK
SIDMLNNISNINKSVKFGYIKKVIDDLNEKVKNSYYQLFETLKSKGYEVVEVQFNQELLDSLSAIYKMISFSESVSTNAN
LDGIKFGQRAAGSDYVEIITNSRTEGFGNEVKQRFLVGALNLNKENQTIYLNKAKQVRRLIVEELNRVFSKTDILIIPPT
DNVAPLIEERKINVSPEKEYLSDILTLANFNGSPSITIPFVKEGKLGIGININAKPKEDLLCLQAAKLLEDIIGIKNEIV
ED

Sequences:

>Translated_482_residues
MNYKNLSILELHNKIINKEITILELTNAVISEANKVKSSNAINKMNEENAIAFSKEMEKVLNTNSLLYGIPYFAKDNFAT
KGLETTSSSNILKGYIPPFNATSISKLEDKGAVLIGKAALDELGMGGVGLYSCNGIITNPADSNRIVGGSSSGSAYLVAK
GIVPFATGSDTGDSIRKPASFNNIVGFKPSYGAISRYGLLPYSPSLDTVGFFTRNVEDMAIVCDATFGYDVKDATSHENK
SIDMLNNISNINKSVKFGYIKKVIDDLNEKVKNSYYQLFETLKSKGYEVVEVQFNQELLDSLSAIYKMISFSESVSTNAN
LDGIKFGQRAAGSDYVEIITNSRTEGFGNEVKQRFLVGALNLNKENQTIYLNKAKQVRRLIVEELNRVFSKTDILIIPPT
DNVAPLIEERKINVSPEKEYLSDILTLANFNGSPSITIPFVKEGKLGIGININAKPKEDLLCLQAAKLLEDIIGIKNEIV
ED
>Mature_482_residues
MNYKNLSILELHNKIINKEITILELTNAVISEANKVKSSNAINKMNEENAIAFSKEMEKVLNTNSLLYGIPYFAKDNFAT
KGLETTSSSNILKGYIPPFNATSISKLEDKGAVLIGKAALDELGMGGVGLYSCNGIITNPADSNRIVGGSSSGSAYLVAK
GIVPFATGSDTGDSIRKPASFNNIVGFKPSYGAISRYGLLPYSPSLDTVGFFTRNVEDMAIVCDATFGYDVKDATSHENK
SIDMLNNISNINKSVKFGYIKKVIDDLNEKVKNSYYQLFETLKSKGYEVVEVQFNQELLDSLSAIYKMISFSESVSTNAN
LDGIKFGQRAAGSDYVEIITNSRTEGFGNEVKQRFLVGALNLNKENQTIYLNKAKQVRRLIVEELNRVFSKTDILIIPPT
DNVAPLIEERKINVSPEKEYLSDILTLANFNGSPSITIPFVKEGKLGIGININAKPKEDLLCLQAAKLLEDIIGIKNEIV
ED

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=36.0160965794769, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI195972892, Length=245, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=429, Percent_Identity=31.7016317016317, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17556264, Length=240, Percent_Identity=30, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=219, Percent_Identity=28.7671232876712, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=409, Percent_Identity=32.0293398533007, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319685, Length=297, Percent_Identity=30.3030303030303, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648113, Length=492, Percent_Identity=32.520325203252, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=25.0501002004008, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=24.1308793456033, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=24.1308793456033, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=24.1308793456033, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=24.1308793456033, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI161078093, Length=519, Percent_Identity=24.0847784200385, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648435, Length=230, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648437, Length=230, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45550774, Length=230, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52851; Mature: 52851

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKNLSILELHNKIINKEITILELTNAVISEANKVKSSNAINKMNEENAIAFSKEMEKV
CCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LNTNSLLYGIPYFAKDNFATKGLETTSSSNILKGYIPPFNATSISKLEDKGAVLIGKAAL
HCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCEEEEHHHH
DELGMGGVGLYSCNGIITNPADSNRIVGGSSSGSAYLVAKGIVPFATGSDTGDSIRKPAS
HHCCCCCEEEEECCCEEECCCCCCEEECCCCCCCEEEEEECCEEECCCCCCCHHHCCCCC
FNNIVGFKPSYGAISRYGLLPYSPSLDTVGFFTRNVEDMAIVCDATFGYDVKDATSHENK
CCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
SIDMLNNISNINKSVKFGYIKKVIDDLNEKVKNSYYQLFETLKSKGYEVVEVQFNQELLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHH
SLSAIYKMISFSESVSTNANLDGIKFGQRAAGSDYVEIITNSRTEGFGNEVKQRFLVGAL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEE
NLNKENQTIYLNKAKQVRRLIVEELNRVFSKTDILIIPPTDNVAPLIEERKINVSPEKEY
ECCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCCCHHHH
LSDILTLANFNGSPSITIPFVKEGKLGIGININAKPKEDLLCLQAAKLLEDIIGIKNEIV
HHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ED
CC
>Mature Secondary Structure
MNYKNLSILELHNKIINKEITILELTNAVISEANKVKSSNAINKMNEENAIAFSKEMEKV
CCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LNTNSLLYGIPYFAKDNFATKGLETTSSSNILKGYIPPFNATSISKLEDKGAVLIGKAAL
HCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCEEEEHHHH
DELGMGGVGLYSCNGIITNPADSNRIVGGSSSGSAYLVAKGIVPFATGSDTGDSIRKPAS
HHCCCCCEEEEECCCEEECCCCCCEEECCCCCCCEEEEEECCEEECCCCCCCHHHCCCCC
FNNIVGFKPSYGAISRYGLLPYSPSLDTVGFFTRNVEDMAIVCDATFGYDVKDATSHENK
CCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
SIDMLNNISNINKSVKFGYIKKVIDDLNEKVKNSYYQLFETLKSKGYEVVEVQFNQELLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHH
SLSAIYKMISFSESVSTNANLDGIKFGQRAAGSDYVEIITNSRTEGFGNEVKQRFLVGAL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEE
NLNKENQTIYLNKAKQVRRLIVEELNRVFSKTDILIIPPTDNVAPLIEERKINVSPEKEY
ECCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCCCHHHH
LSDILTLANFNGSPSITIPFVKEGKLGIGININAKPKEDLLCLQAAKLLEDIIGIKNEIV
HHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ED
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]