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The map label for this gene is gltJ [H]

Identifier: 50085185

GI number: 50085185

Start: 2044708

End: 2045409

Strand: Reverse

Name: gltJ [H]

Synonym: ACIAD2059

Alternate gene names: 50085185

Gene position: 2045409-2044708 (Counterclockwise)

Preceding gene: 50085186

Following gene: 50085184

Centisome position: 56.84

GC content: 41.74

Gene sequence:

>702_bases
TTGCAAATGCTAGGTTCTGGTGTGGTCACCATGATCTGGACGGCAGTAGCCGCATTTTTAATCGCTTTTTTCTTAGGCTC
GTTATTAGGGATTATCCGCACTTTGCCAAATAAACCTTTGGCATTGATTGGCGATACCTATGTTGAAATTTTTCGTAATA
TTCCTTTAATTGTTCAATTATTCTTTTGGGCTTTTGTATTTCCTGAGTTATTACCCGCTTCACTGAGCTCTGGTAGTGGA
GAGGTTGTCGCAGGAGGTTGGTGGAAAAATATCCTGAATAATCATCCTGCTGTGATTGGTGTGTTTGCATTGGGGCTATA
TACGGCTGCACGTGTCTCGGAACATATTCGCGCAGGTATTAATACCGTTTCAAGTGGTCAAAAATTTGCTGCTTCTGCAA
TGGGTTTCACCACAGGTCAAAGCTATCGTTATGTCATTCTCCCAGTCGCATACCGTACTGTGTGGCCGACAATTACGTCT
GAAGCGATGAATGTGTTTAAAAACTCGGCTGTGTTATATGCATTAAGTGTATTAAACTTTTTTGCATATACCAAAACCAT
GCGTGAAGAAACCTCGCAAGACATCATCATCCTTATTCTCTCTACACCTGTCTATTTGGTGATCACCTATACCATTAAAT
TTATCATGGCATGGATTGAAAAGCGTATGGCTGTTCCTGGCCTCGGTTCAGGAGGGAAGTAA

Upstream 100 bases:

>100_bases
ATCGAATGAATTTAGTGTCGATAAAGCAGCTTGAGCGGAATCGGTCTGTAAAGCGATAGGCAGCTCAAGTTATTCTCAAT
ATGCCGATGCACCAACTTGG

Downstream 100 bases:

>100_bases
TCATGGATTTTAGTCTATTACAGCATCCTGATGTAACACATGCGTTAAGTGAAGGATTTATCTTCACGCTGACCGTAACC
GTACTTGCAATGATTGGTGG

Product: glutamate/aspartate ABC transporter

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MQMLGSGVVTMIWTAVAAFLIAFFLGSLLGIIRTLPNKPLALIGDTYVEIFRNIPLIVQLFFWAFVFPELLPASLSSGSG
EVVAGGWWKNILNNHPAVIGVFALGLYTAARVSEHIRAGINTVSSGQKFAASAMGFTTGQSYRYVILPVAYRTVWPTITS
EAMNVFKNSAVLYALSVLNFFAYTKTMREETSQDIIILILSTPVYLVITYTIKFIMAWIEKRMAVPGLGSGGK

Sequences:

>Translated_233_residues
MQMLGSGVVTMIWTAVAAFLIAFFLGSLLGIIRTLPNKPLALIGDTYVEIFRNIPLIVQLFFWAFVFPELLPASLSSGSG
EVVAGGWWKNILNNHPAVIGVFALGLYTAARVSEHIRAGINTVSSGQKFAASAMGFTTGQSYRYVILPVAYRTVWPTITS
EAMNVFKNSAVLYALSVLNFFAYTKTMREETSQDIIILILSTPVYLVITYTIKFIMAWIEKRMAVPGLGSGGK
>Mature_233_residues
MQMLGSGVVTMIWTAVAAFLIAFFLGSLLGIIRTLPNKPLALIGDTYVEIFRNIPLIVQLFFWAFVFPELLPASLSSGSG
EVVAGGWWKNILNNHPAVIGVFALGLYTAARVSEHIRAGINTVSSGQKFAASAMGFTTGQSYRYVILPVAYRTVWPTITS
EAMNVFKNSAVLYALSVLNFFAYTKTMREETSQDIIILILSTPVYLVITYTIKFIMAWIEKRMAVPGLGSGGK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=230, Percent_Identity=40.4347826086956, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786873, Length=226, Percent_Identity=28.3185840707965, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994930, Length=125, Percent_Identity=35.2, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788645, Length=218, Percent_Identity=27.0642201834862, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787087, Length=215, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788226, Length=209, Percent_Identity=22.488038277512, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787086, Length=224, Percent_Identity=24.5535714285714, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787030, Length=226, Percent_Identity=23.0088495575221, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788646, Length=221, Percent_Identity=23.0769230769231, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25517; Mature: 25517

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMLGSGVVTMIWTAVAAFLIAFFLGSLLGIIRTLPNKPLALIGDTYVEIFRNIPLIVQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHH
FFWAFVFPELLPASLSSGSGEVVAGGWWKNILNNHPAVIGVFALGLYTAARVSEHIRAGI
HHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NTVSSGQKFAASAMGFTTGQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNF
HHHCCCHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAYTKTMREETSQDIIILILSTPVYLVITYTIKFIMAWIEKRMAVPGLGSGGK
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQMLGSGVVTMIWTAVAAFLIAFFLGSLLGIIRTLPNKPLALIGDTYVEIFRNIPLIVQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHH
FFWAFVFPELLPASLSSGSGEVVAGGWWKNILNNHPAVIGVFALGLYTAARVSEHIRAGI
HHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NTVSSGQKFAASAMGFTTGQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNF
HHHCCCHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAYTKTMREETSQDIIILILSTPVYLVITYTIKFIMAWIEKRMAVPGLGSGGK
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]