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The map label for this gene is gltK [H]

Identifier: 50085184

GI number: 50085184

Start: 2044028

End: 2044705

Strand: Reverse

Name: gltK [H]

Synonym: ACIAD2058

Alternate gene names: 50085184

Gene position: 2044705-2044028 (Counterclockwise)

Preceding gene: 50085185

Following gene: 50085183

Centisome position: 56.82

GC content: 38.35

Gene sequence:

>678_bases
ATGGATTTTAGTCTATTACAGCATCCTGATGTAACACATGCGTTAAGTGAAGGATTTATCTTCACGCTGACCGTAACCGT
ACTTGCAATGATTGGTGGTATTGTCATTGGTACGCCTCTGGCGATGATGCGTTTATCAAATAACGTTGTTGCGAGTAATT
TTGCAAAATTTTATGTGGATGTATTTCGCGGTATTCCTTTAATTCAGGTTATTTTTATTTTTTATTTCCTGTTGCCTAAA
GTGTTTGAATTCCAATCTGACACTTATTGGGGGCCTTTATTTTCAAGTGTGATTACATTTGCAATTTTTGAAGCCGCATT
CTTCTCAGAAATTGTACGTTCAGGTATCCAGTCGGTATCTCGTGGTCAAGTCAATGCAGGTTATGCGCTAGGTTTTACCT
ATGGCCAATCGATGAAATATGTTGTTTTGCCTCAGGCATTTAGAAACATGTTACCTGTATTACTCACTCAAACGATTATC
CTGTTTCAAGACGTTTCTTTGGTTTATGTCATCAGTGCACCAGATTTCCTTGGTCGGGCAGATACGTTGGCCAATACATA
CGGTCCAGAAACTAAAGCAACCTTTTATTTAATTGTAGCTGTGGTTTATTTCTGTAGTTCGTTCCTGCTTTCACGCTTGG
TTAAACGATTACAGCAAAAAATTGCCATTATTCGCTGA

Upstream 100 bases:

>100_bases
CACCTGTCTATTTGGTGATCACCTATACCATTAAATTTATCATGGCATGGATTGAAAAGCGTATGGCTGTTCCTGGCCTC
GGTTCAGGAGGGAAGTAATC

Downstream 100 bases:

>100_bases
TTGGAGATTTTTAAATGCCAATGATAGACATCCAACATATCTCTAAATGGTATGGTGATTTCCAAGTACTCACTGACTGT
ACAACAAGTATTGAAAAAGG

Product: glutamate/aspartate ABC transporter

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDFSLLQHPDVTHALSEGFIFTLTVTVLAMIGGIVIGTPLAMMRLSNNVVASNFAKFYVDVFRGIPLIQVIFIFYFLLPK
VFEFQSDTYWGPLFSSVITFAIFEAAFFSEIVRSGIQSVSRGQVNAGYALGFTYGQSMKYVVLPQAFRNMLPVLLTQTII
LFQDVSLVYVISAPDFLGRADTLANTYGPETKATFYLIVAVVYFCSSFLLSRLVKRLQQKIAIIR

Sequences:

>Translated_225_residues
MDFSLLQHPDVTHALSEGFIFTLTVTVLAMIGGIVIGTPLAMMRLSNNVVASNFAKFYVDVFRGIPLIQVIFIFYFLLPK
VFEFQSDTYWGPLFSSVITFAIFEAAFFSEIVRSGIQSVSRGQVNAGYALGFTYGQSMKYVVLPQAFRNMLPVLLTQTII
LFQDVSLVYVISAPDFLGRADTLANTYGPETKATFYLIVAVVYFCSSFLLSRLVKRLQQKIAIIR
>Mature_225_residues
MDFSLLQHPDVTHALSEGFIFTLTVTVLAMIGGIVIGTPLAMMRLSNNVVASNFAKFYVDVFRGIPLIQVIFIFYFLLPK
VFEFQSDTYWGPLFSSVITFAIFEAAFFSEIVRSGIQSVSRGQVNAGYALGFTYGQSMKYVVLPQAFRNMLPVLLTQTII
LFQDVSLVYVISAPDFLGRADTLANTYGPETKATFYLIVAVVYFCSSFLLSRLVKRLQQKIAIIR

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=214, Percent_Identity=46.7289719626168, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787030, Length=210, Percent_Identity=36.6666666666667, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788226, Length=207, Percent_Identity=32.8502415458937, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786874, Length=210, Percent_Identity=29.0476190476191, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082239, Length=206, Percent_Identity=28.1553398058252, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788645, Length=216, Percent_Identity=28.2407407407407, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787087, Length=218, Percent_Identity=24.3119266055046, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788646, Length=198, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994930, Length=131, Percent_Identity=32.0610687022901, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787086, Length=215, Percent_Identity=21.3953488372093, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25184; Mature: 25184

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSLLQHPDVTHALSEGFIFTLTVTVLAMIGGIVIGTPLAMMRLSNNVVASNFAKFYVD
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VFRGIPLIQVIFIFYFLLPKVFEFQSDTYWGPLFSSVITFAIFEAAFFSEIVRSGIQSVS
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGQVNAGYALGFTYGQSMKYVVLPQAFRNMLPVLLTQTIILFQDVSLVYVISAPDFLGRA
CCCCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHH
DTLANTYGPETKATFYLIVAVVYFCSSFLLSRLVKRLQQKIAIIR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFSLLQHPDVTHALSEGFIFTLTVTVLAMIGGIVIGTPLAMMRLSNNVVASNFAKFYVD
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VFRGIPLIQVIFIFYFLLPKVFEFQSDTYWGPLFSSVITFAIFEAAFFSEIVRSGIQSVS
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGQVNAGYALGFTYGQSMKYVVLPQAFRNMLPVLLTQTIILFQDVSLVYVISAPDFLGRA
CCCCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHH
DTLANTYGPETKATFYLIVAVVYFCSSFLLSRLVKRLQQKIAIIR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]